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Genetics and Breeding for Modified Fatty Acid Profile in Soybean Seed Oil

  • Lee, Jeong-Dong;Bilyeu, Kristin D.;Shannon, James Grover
    • Journal of Crop Science and Biotechnology
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    • 제10권4호
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    • pp.201-210
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    • 2007
  • Soybean [Glycine max(L.) Merr.] oil is versatile and used in many products. Modifying the fatty acid profile would make soy oil more functional in food and other products. The ideal oil with the most end uses would have saturates(palmitic + stearic acids) reduced from 15 to < 7%, oleic acid increased from 23 to > 55%, and linolenic acid reduced from 8 to < 3%. Reduced palmitic acid(16:0) is conditioned by three or more recessive alleles at the Fap locus. QTLs for reduced palmitic acid have mapped to linkage groups(LGs) A1, A2, B2, H, J, and L. Genes at the Fad locus control oleic acid content(18:1). Six QTLs($R^2$=4-25%) for increased 18:1 in N00-3350(50 to 60% 18:1) explained four to 25% of the phenotypic variation. M23, a Japanese mutant line with 40 to 50% 18:1 is controlled by a single recessive gene, ol. A candidate gene for FAD2-1A can be used in marker-assisted breeding for high 18:1 from M23. Low linolenic acid(18:3) is desirable in soy oil to reduce hydrogenation and trans-fat accumulation. Three independent recessive genes affecting omega-3 fatty acid desaturase enzyme activity are responsible for the lower 18:3 content in soybeans. Linolenic acid can be reduced from 8 to about 4, 2, and 1% from copies of one, two, or three genes, respectively. Using a candidate gene approach perfect markers for three microsomal omega-3 desaturase genes have been characterized and can readily be used in for marker assisted selection in breeding for low 18:3.

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수리실험을 통한 요철형 인공어초 안정성 검토 (Hydraulic model test for corrugated artificial reef stability)

  • 백승화;신범식;김규한
    • 한국산학기술학회논문지
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    • 제15권8호
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    • pp.5327-5332
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    • 2014
  • 우리나라 연안생태계는 백화현상(갯녹음)에 의한 해조류의 감소와 어패류 등 어업자원의 감소로 인해 연안역의 황폐화에 직면해 있다. 새로운 유형의 인공어초에 대한 안정성을 평가하기 위해 본 연구에서는 2차원 단면수조를 이용하여 새롭게 개발된 인공어초의 설치수심에 따른 재현파고, 주기, 흐름 등 외력을 변화시켜 요철형 어초의 안정 조건이 되는 한계치를 구하고, 설계외력(파랑, 흐름 등)의 안정성에 대한 검토를 수행하였다. 본 연구 결과는 새로운 인공어초의 설계 및 실해역에 시공하기 위해 구조물의 안정성을 사전에 검토할 수 있는 있는 기초자료를 제공할 수 있을 것으로 판단된다.

기상요인이 콩 단백질 함량에 미치는 영향 (Climatic Influence on Seed Protein Content in Soybean(Glycine max))

  • 양무희
    • 한국작물학회지
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    • 제42권5호
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    • pp.539-547
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    • 1997
  • This study was carried out to identify how soybean seed protein concentration is influenced by climatic factors. Twelve lines selected for seed protein concentration were studied in 13 environments of North Carolina. Sensitivity of seed protein concentration, total seed protein, and seed yield to climatic variables was investigated using a linear regression model. Best response models were determined using two stepwise selection methods, Maximum R-square and Stepwise Selection. There were wide climatic effects in seed protein concentration, total protein and seed yield. The highest protein concentration environment was characterized by the most high temperature days(HTD) and the smallest variance of average daily temperature range (VADTRg), while the lowest protein concentration environment was distinguished by the fewest HTD and the largest VADTRg. For protein concentration, all lines responded positively to average maximum daily temperature(MxDT), HTD, and average daily temperature range(ADTRg) and negatively to ADRa, while they responded positively or negatively to average daily temperature(ADT), variance of average minimum daily temperature (VMnDT), and VADTRg, indicating that genotypes may greatly differ in degrees of sensitivity to each climatic variable. Eleven lines seemed to have best response models with 2 or 3 variables. Exceptionally, NC106 did not show a significant sensitivity to any climatic variable and thus did not have a best response model. This indicates that it may be considered phenotypically more stable. For total seed protein and seed yield, all the lines responded negatively to both ADTRg and VADRa, suggesting that synthesis of seed components may increase with less daily temperature range and less variation in daily rainfall.

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The Role of Magnetic Topology in the Heating of Active Region Coronal Loops

  • Lee, Jin-Yi;Barnes, Graham;Leka, K.D.;Reeves, Katharine K.;Korreck, K.E.;Golub, L.;Deluca, E.E.
    • 천문학회보
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    • 제36권2호
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    • pp.86.2-86.2
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    • 2011
  • We investigate the evolution of coronal loop emission in the context of the coronal magnetic field topology. New modeling techniques allow us to investigate the magnetic field structure and energy release in active regions (ARs). Using these models and high-resolution multi-wavelength coronal observations from the Transition Region and Coronal Explorer and the X-ray Telescope on Hinode, we are able to establish a relationship between the light curves of coronal loops and their associated magnetic topologies for NOAA AR 10963. We examine loops that show both transient and steady emission, and we find that loops that show many transient brightenings are located in domains associated with a high number of separators. This topology provides an environment for continual impulsive heating events through magnetic reconnection at the separators. A loop with relatively constant X-ray and EUV emission, on the other hand, is located in domains that are not associated with separators. This result implies that larger-scale magnetic field reconnections are not involved in heating plasma in these regions, and the heating in these loops must come from another mechanism, such as smallscale reconnections (i.e., nanoflares) or wave heating. Additionally, we find that loops that undergo repeated transient brightenings are associated with separators that have enhanced free energy. In contrast, we find one case of an isolated transient brightening that seems to be associated with separators with a smaller free energy.

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닭 백혈구 특이 단트론항체를 이용한 한국 재래닭의 유전 면역학적 특성에 관한 연구 (Immunogenetic characterization of Korean native chickens by monoclonal anibodies to chicken leukocyte differentiation antigens)

  • 박용호;한재용;오봉국;문진산;구복경;주이석;서근석
    • 대한수의학회지
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    • 제38권1호
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    • pp.91-99
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    • 1998
  • The immunogenetic analysis was performed to characterize the Korean native chickens (KNC) determined by monoclonal antibodies specific to chicken leukocyte differentiation antigens and flow cytometry. A total of 174 chickens including 58 KNC (black, brown and darkbrown colored), 77 foreign breed (Nagoya, White Reghorn, Rhode Island and Cornish) and 39 mixed breed (19 KNC with Nagoya and 20 KNC with Rhode Island) separately growing at Animal Science and Technology Institute were examined. The proportion of cells expressing MHC class II molecule (B-L in chicken) was significantly high in KNC. Proportion of CD4+ T helper cells was also higher in KNC and two mixed breed than that in foreign breed. However, proportion of CD8+ cells and TCR1 + (${\gamma}^{\delta}$ T cell receptor) cells was the lowest among the breed examined. Otherwise, those proportions were significantly high in White leghorn and two mixed breeds with two exclusive subpopulations. The two subpopulations were also typically shown in MHC class $II^+$ cells in KNC and one mixed breed, black-colored KNC with Nagoya. Although genotypic analysis was not pursued to characterize the immunogenetic properties of KNC, difference of phenotypic expression based on leukocyte differentiation molecules could be elucidated in KNC in this study.

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The Endophyte Curtobacterium flaccumfaciens Reduces Symptoms Caused by Xylella fastidiosa in Catharanthus roseus

  • Lacava, Paulo Teixeira;Li, Wenbin;Araujo, Welington Luiz;Azevedo, Joao Lucio;Hartung, John Stephen
    • Journal of Microbiology
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    • 제45권5호
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    • pp.388-393
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    • 2007
  • Citrus variegated chlorosis (CVC) is a disease of the sweet orange [Citrus sinensis (L.)], which is caused by Xylella fastidiosa subsp. pauca, a phytopathogenic bacterium that has been shown to infect all sweet orange cultivars. Sweet orange trees have been occasionally observed to be infected by Xylella fastidiosa without evidencing severe disease symptoms, whereas other trees in the same grove may exhibit severe disease symptoms. The principal endophytic bacterial species isolated from such CVC-asymptomatic citrus plants is Curtobacterium flaccumfaciens. The Madagascar periwinkle [Citrus sinensis (L.)] is a model plant which has been used to study X. fastidiosa in greenhouse environments. In order to characterize the interactions of X. fastidiosa and C. flaccumfaciens, periwinkle plants were inoculated separately with C. flaccumfaciens, X. fastidiosa, and both bacteria together. The number of flowers produced by the plants, the heights of the plants, and the exhibited disease symptoms were evaluated. PCR-primers for C. flaccumfaciens were designed in order to verify the presence of this endophytic bacterium in plant tissue, and to complement an existing assay for X. fastidiosa. These primers were capable of detecting C. flaccumfaciens in the periwinkle in the presence of X. fastidiosa. X. fastidiosa induced stunting and reduced the number of flowers produced by the periwinkle. When C. flaccumfaciens was inoculated together with X. fastidiosa, no stunting was observed. The number of flowers produced by our doubly- inoculated plants was an intermediate between the number produced by the plants inoculated with either of the bacteria separately. Our data indicate that C. flaccumfaciens interacted with X. fastidiosa in C. roseus, and reduced the severity of the disease symptoms induced by X. fastidiosa. Periwinkle is considered to be an excellent experimental system by which the interaction of C. flaccumfaciens and other endophytic bacteria with X. fastidiosa can be studied.

Effects of Noradrenaline on the Membrane Potential of Prostatic Neuroendocrine Cells of Rat

  • Kim, Jun-Hee;Shin, Sun-Young;Uhm, Dae-Yong;Kim, Sung-Joon
    • The Korean Journal of Physiology and Pharmacology
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    • 제7권1호
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    • pp.47-52
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    • 2003
  • The prostate gland contains numerous neuroendocrine cells that are believed to influence the function of the prostate gland. Our recent study demonstrated the expression of both ${\alpha}1$- and ${\alpha}2$-ARs, signaling the release of stored $Ca^{2+}$ and the inhibition of N-type $Ca^{2+}$ channels, respectively, in rat prostate neuroendocrine cells (RPNECs). In this study, the effects of NA on the resting membrane potential (RMP) of RPNECs were investigated using a whole-cell patch clamp method. Fresh RPNECs were dissociated from the ventral lobe of rat prostate and identified from its characteristic shape; round or oval shape with dark cytoplasm. Under zero-current clamp conditions with KCl pipette solution, the resting membrane potential (RMP) of RPNECs was between -35 mV and -85 mV. In those RPNECs with relatively hyperpolarized RMP (<-60 mV), the application of noradrenaline (NA, $1{\mu}M$) depolarized the membrane to around -40 mV. In contrast, the RPNECs with relatively depolarized RMP (>-45 mV) showed a transient hyperpolarization and subsequent fluctuation at around -40 mV on application of NA. Under voltage clamp conditions (holding voltage, -40 mV) with CsCl pipette solution, NA evoked a slight inward current (<-20 pA). NA induced a sharp increase of cytosolic $Ca^{2+}$ concentration ($[Ca^{2+}]_c$), measured by the fura-2 fluorescence, and the voltage clamp study showed the presence of charybdotoxin-sensitive $Ca^{2+}$-activated $K^+$ currents. In summary, adrenergic stimulation induced either depolarization or hyperpolarization of RPNECs, depending on the initial level of RMP. The inward current evoked by NA and the $Ca^{2+}$-activated $K^+$ current might partly explain the depolarization and hyperpolarization, respectively.

Development of the Korean Form of the Premonitory Urge for Tics Scale: A Reliability and Validity Study

  • Kim, Mira;Chung, Sang-Keun;Yang, Jong-Chul;Park, Jong-Il;Nam, Seok Hyun;Park, Tae Won
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • 제31권3호
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    • pp.146-153
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    • 2020
  • Objectives: This study aimed to evaluate the reliability and validity of the Korean Form of the Premonitory Urge for Tics Scale (K-PUTS). Methods: Thirty-eight patients with Tourette's disorder who visited Jeonbuk National University Hospital were assessed with the K-PUTS. Together with the PUTS, the Yale Global Tic Severity Scale (YGTSS), the Children's Yale-Brown Obsessive Compulsive Scale (CY-BOCS), the attention-deficit/hyperactivity disorder (ADHD) rating scale (ARS), and the Adult ADHD Self-Report Scale (ASRS) were implemented to evaluate concurrent and discriminant validity. Results: The internal consistency of items on the PUTS was high, with a Cronbach's α of 0.79. The test-retest reliability of the PUTS, which was administered at 2 weeks to 2 months intervals, showed high reliability with a Pearson correlation coefficient of 0.60. There was a significant positive correlation between the overall PUTS score and the YGTSS score, showing concurrent validity. There was no correlation between the PUTS, CY-BOCS, and ASRS scores, demonstrating the discriminant validity of the PUTS. Factor analysis for construct validity revealed three factors: "presumed functional relationship between the tic and the urge to tic," "the quality of the premonitory urge," and "just right phenomena." Conclusion: The results of this study indicate that the K-PUTS is a reliable and valid scale for rating premonitory urge of tics.

IEEE 802.11i 무선 랜 보안을 위한 AES 기반 CCMP 코어 설계 (A Design of AES-based CCMP core for IEEE 802.11i Wireless LAN Security)

  • 황석기;김종환;신경욱
    • 한국통신학회논문지
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    • 제31권6A호
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    • pp.640-647
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    • 2006
  • 본 논문에서는 IEEE 802.11i 무선 랜 보안을 위한 AES(Advanced Encryption Standard) 기반 CCMP (Counter mode with CBC-MAC Protocol) 코어의 설계에 대해서 기술한다. 설계된 CCMP 코어는 데이터의 기밀성을 위한 CTR(counter) 모드와 인증 및 데이터 무결성 검증을 위한 CBC 모드의 동작이 두개의 AES 암호 코어로 병렬처리 되도록 설계되어 전체 성능의 최적화를 이루었다. AES 암호 코어에서 하드웨어 복잡도에 가장 큰 영향을 미치는 S-box를 composite field 연산 방식을 적용하여 설계함으로써 기존의 LUT(Lookup Table) 기반의 구현방식에 비해 게이트 수가 약 27% 감소되도록 하였다. 설계된 CCMP 코어는 Excalibur SoC 장비를 이용하여 H/W-S/W 통합 검증을 수행하였으며, 0.35-um CMOS 표준 셀 공정으로 MPW 칩으로 제작하고, 제작된 칩의 테스트 결과 모든 기능이 정상 동작함을 확인하였다. 설계된 CCMP 프로세서는 약 17,000개의 게이트로 구현되었으며, 116-MHz@3.3-V의 클록으로 안전하게 동작하여 353-Mbps의 성능이 예상되어 IEEE 802.11a와 802.11g 표준의 MAC 성능인 54-Mbps를 만족한다.

채취 시기가 다른 표고버섯의 급여가 KK 당뇨 마우스의 혈당과 혈청 지질 수준에 미치는 영향 (Supplementary Effect by Harvest period of Lentinus edodes on the levels of Blood glucose and Serum lipid in Diabetic KK mice)

  • 이성현;박흥주;조소영;정현진;조수묵
    • 한국지역사회생활과학회지
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    • 제16권1호
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    • pp.105-111
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    • 2005
  • This study was carried out to investigate the effect of Lentinus edodes which were harvested at different times of maturity on blood glucose and lipid levels in diabetic mice. The diabetic KK mice were fed diets supplemented with Lentinus edodes harvested early (LE) or late (LL) for eight weeks, and control (CO) mice were fed regular diet without Lentinus edodes. Diet intake, body weight, organ weight, blood glucose and serum lipid levels were measured. The mice which were fed diets supplemented with LE and LL showed significantly lower body weights compared to the CO group. There were no significant differences in the diet intake and the weights of different organs including liver, heart, kidneys, and epididymal fat pad among three different groups. Furthermore, the concentrations of serum triglycerides and total cholesterol were significantly lower in LE and LL groups compared to the CO group. These results clearly demonstrate that Lentinus edodes, regardless of their harvested period reduced body weight and serum lipid without affecting blood glucose level in diabetic mice.

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