• Journal of Microbiology and Biotechnology
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• v.22 no.6
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• pp.780-787
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• 2012

The maize pathogen Gibberella moniliformis produces fumonisins, a group of mycotoxins associated with several disorders in animals and humans, including cancer. The current focus of our research is to understand the regulatory mechanisms involved in fumonisin biosynthesis. In this study, we employed a proteomics approach to identify novel genes involved in the fumonisin biosynthesis under nitrogen stress. The combination of genome sequence, mutant strains, EST database, microarrays, and proteomics offers an opportunity to advance our understanding of this process. We investigated the response of the G. moniliformis proteome in limited nitrogen (N0, fumonisin-inducing) and excess nitrogen (N+, fumonisin-repressing) conditions by one- and two-dimensional electrophoresis. We selected 11 differentially expressed proteins, six from limited nitrogen conditions and five from excess nitrogen conditions, and determined the sequences by peptide mass fingerprinting and MS/MS spectrophotometry. Subsequently, we identified the EST sequences corresponding to the proteins and studied their expression profiles in different culture conditions. Through the comparative analysis of gene and protein expression data, we identified three candidate genes for functional analysis and our results provided valuable clues regarding the regulatory mechanisms of fumonisin biosynthesis.

• Journal of Fisheries and Marine Sciences Education
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• v.28 no.4
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• pp.1042-1056
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• 2016

To evaluate the wake regions of six artificial reefs (ARs) frequently used in the marine forest creation project in Korea, we consider the effect of water flow directions on the wake regions and accordingly propose a wake region diagram, which is characterized by parameters such as wake volume fluctuations, averaged wake volume, fundamental symmetric angle, secure angle, and principal direction. To demonstrate the parameters, seven water flow directions (0 to $90^{\circ}$) were considered and consequently the variations in wake volumes were investigated by using the concept of wake volume, adopting element-based finite volume method, and utilizing numerical flow domain and boundary conditions. From the analysis results, it was shown that the wake region diagrams have a period of either 45 or $90^{\circ}$ according to the geometrical symmetry of each artificial reef. Also, it was found that the secure angle ranges fluctuate depending on the shapes and sizes of the artificial reefs considered. Thus, it is demanded to consider those parameters during installation of artificial reefs for establishing a larger wake region and accordingly attracting more marine fauna and flora in the region.

• Journal of the Society of Naval Architects of Korea
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• v.42 no.6 s.144
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• pp.566-574
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• 2005

Reduction of a ship's rolling is the most important performance requirement for improving the safety of the crew on board and preventing damage to cargos as well as improving the comfort of the ride. A mass driving anti-rolling system (MO-ARS) might be one candidate of several systems against the ship's rolling. As the movable range of the mass on the ship is finite, the control system must include restriction on the mass position to protect the device and the ship. This restriction usually causes windup phenomenon and control performance is deteriorated seriously. Two control algorithms, anti-windup control and saturated sliding mode control, are studied in this paper. Control performance and robustness problem are checked out by numerical simulations.

• Anxiety and mood
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• v.14 no.1
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• pp.1-6
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• 2018

Objective : This study aimed at investigating the relationship between exposure frequency of job related trauma types and the PTSD symptoms severity of firefighters, in relation to the moderating effect that anger rumination plays in the association between those of them. Methods : We examined data from 116 firefighters from Gyeonggi-do. The following instruments were applied: the Life Events Checklist (LEC), the Korean version of the Anger Rumination Scale (K-ARS) and the Primary Care PTSD screen (PC-PTSD). Results : A higher exposure frequency of job related trauma types was associated with severe PTSD symptoms; we discovered, however, that anger rumination exacerbated their relationship. Conclusion : This study showed that anger rumination is one of the variables to exacerbate PTSD symptoms in firefighters. Therefore, the study emphasizes the need to consider the level of anger rumination when running a program to predict or mitigate PTSD symptoms in firefighters.

• Journal of Auto-vehicle Safety Association
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• v.5 no.1
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• pp.37-43
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• 2013

This paper describes an investigation into coordinated control of electronic stability control (ESC) and active roll control system (ARS). The coordinated control is suggested to improve the vehicle stability and agility features by yaw rate control. The proposed integrated chassis control algorithm consists of a supervisor, control algorithms, and a coordinator. The supervisor monitors the vehicle status and determines desired vehicle motions such as a desired yaw rate and desired roll motion based on control modes to improve vehicle stability. According to the corresponding the desired vehicle dynamics, the control algorithm calculated a desired yaw moment and desired roll moment, respectively. Based on the desired yaw moment and the desired roll moment, the coordinator determines the brake pressures and the ARC motor torques based on control strategies. Closed loop simulations with a driver-vehicle-controller system were conducted to investigate the performance of the proposed control strategy using CarSim vehicle dynamics software and the integrated controller coded using Matlab/Simulink.

• Hwankyungkyoyuk
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• v.23 no.2
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• pp.113-128
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• 2010

This study investigated ADHD prevalence among the first to sixth graders in ordinary classes of H elementary school located in an area of special rural conditions and selected 4 sixth graders who received high scores in a self-diagnostic test on attention deficit and over 17 points from teachers in an ARS-IV test with a view to verifying the effects of the education in environment on the improvement of maladjusted behaviors among ADHD children when the education program for improvement of maladjusted behaviors is applied to the environment. The program was applied from March to mid-July for about 4.5 months in 13 periods. The program applied consisted of activities of experiencing the nature. Behavior changes of targets during the course of the program were recorded in the frequency of ADHD target behaviors card and the observation log to describe the results, which underwent a comparative analysis through pre-and post-tests of ADDES-SV and to graph the changes in maladjusted behaviors of target children. To sum up, the education in environment resulted in positive effects on the changes of maladjusted behaviors among ADHD children by reducing attention deficit impulsive behaviors and hyperactivity.

• Molecules and Cells
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• v.21 no.2
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• pp.213-217
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• 2006

Although AIMP1 (previously known as p43) is one of three auxiliary proteins bound to a macromolecular aminoacyl tRNA complex, it is also secreted as a cytokine controlling both angiogenesis and immune responses. Here we show that systemically administered purified recombinant human AIMP1 had anti-tumor activity in mouse xenograft models. In Meth A-bearing Balb/c mice, tumor volume increased about 28 fold in the vehicle treatment group, while an increase of about 16.7 fold was observed in the AIMP1-treated group. We also evaluated the anti-tumor activity of AIMP1 in combination with a sub-clinical dose of the cytotoxic anti-tumor drug, paclitaxel. The growth of NUGC-3 human stomach cancer cells was suppressed by 84% and 94% by the combinations of 5 mg/kg paclitaxel + 25 mg/kg AIMP1 (p = 0.03), and 5 mg/kg paclitaxel + 50 mg/kg AIMP1 (p = 0.02), respectively, while 5 mg/kg paclitaxel alone suppressed growth by only 54% (p = 0.02). A similar cooperative effect of AIMP1 and paclitaxel was observed in a lung cancer xenograft model. These results suggest that AIMP1 may be useful as a novel anti-tumor agent.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.5
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• pp.392-396
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• 2003

Maysin, a C-glycosylflavone, was isolated from the silks of maize, Zea mays L. The ESI mass spectrum indicates that molecular weight of maysin is $577\textrm{M}^+$m/z, and the ether-linked sugar is rhamnose, $431\textrm{M}^+$m/z (MW$^{+}$-146). The DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging activity of maysin was higher than that of rutin. However, as compared with its aglycon luteolin, maysin showed the relatively moderate DPPH scavenging activity mainly due to the glycosylation of two sugars moieties, keto-fucose and rhamnose. In the in vitro cytotoxicity test against the five human tumor cell lines such as lung (A549), ovarian (SK-OV-3), melanoma (SK-MEL-2), central nerve system (XF-489), and colon (HCT-15), maysin exhibited the relatively weaker activities than cisplatin. The $\textrm{ED}_{50}$ values of maysin were 62.24, 43.18, 16.83, 37.22, and 32.09/$m\ell$, respectively. Result suggests that maysin is a potential cytotoxicity compound, particularly for human colon, central nerve system, and melanoma tumors.s.

• Journal of Crop Science and Biotechnology
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• v.11 no.3
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• pp.177-180
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• 2008

Worldwide, cyst nematode(Heterodera glycines Ichinohe) is the most destructive pathogen of cultivated soybean. In the USA, current annual yield losses are estimated to be nearly a billion dollars. Crop losses are primarily reduced by the use of resistant cultivars. Nematode populations are variable and have adapted to reproduce on resistant cultivars over time because resistance primarily traces to two soybean accessions. It is important to use diverse resistance sources to develop new nematode resistant cultivars. Soybean PI 494182 is a recent introduction from Japan and found to be resistant to multiple nematode populations. It is yellow seeded and maturity group 0. We have determined inheritance of resistance in PI 494182 using $F_{2:3}$ families derived from cross PI 494182 X cv. Skylla. Skylla is a susceptible parent. Three nematode populations, races 1, 3, and 5, corresponding to HG types 2.5.7, 0, and 2.5.7 were used to bioassay 162 $F_{2:3}$ families in greenhouse experiments. Based on Chi-square tests, a two-gene model is proposed for resistance to race 1 and a three-gene model is proposed for conditioning resistance to both races 3 and 5. Correlation coefficient analysis indicated that some genes conditioning resistance to races 1, 3, and 5 are shared or closely linked with each other. These results will be useful to soybean breeders for developing soybean cultivars for broad resistance to nematodes.

• Proceedings of the Optical Society of Korea Conference
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• 2000.08a
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• pp.62-63
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• 2000

다양한 분야의 요구에 따라 반사율이 99.995% 이상인 고반사 저손실 반사경의 제작이 가능해 지면서 반사경의 산란, 투과, 흡수 및 손실 등을 측정하는 여러 측정 방법들도 더불어 연구되어 왔다. 적용 분야에 따라 사용될 반사경의 특성이 결정되는데 그 중 중력파 측정 장치$^{(1)}$ , 광자 감쇠 분광기$^{(2)}$ 등의 분야에서는 투과, 산란 및 흡수가 모두 작은 저손실 반사경을 요구한다. 한편 링 레이저 자이로스코프의 경우 반사경의 산란이 곧 lock-in을 결정하여 성능을 제한하는 중요한 변수가 된다. 따라서 이 응용의 경우 반사경의 산란을 정확히 측정하는 일은 중요하며 위치에 따른 산란분포 정보를 알면 링 레이저 자이로스코프의 성능 예측과 개선이 더욱 쉬워진다. 산란을 측정하는 직접적인 방법에는 ARS(angle resolved scattering)과 TIS(total integrated scattering)이 있는데, 본 연구에서는 TIS 측정 장비를 반사경의 위치에 따른 산란분포까지 측정할 수 있도록 mapping 기능을 첨가하고 이온빔 스퍼터링에 의해 제작된 저산란 반사경을 측정하기 위해 높은 분해능을 갖도록 구성하였다. 반사경의 응용분야의 필요에 의해 45$^{\circ}$ 산란을 측정하였다. (중략)