• The Korea Journal of Herbology
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• v.30 no.4
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• pp.71-79
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• 2015

Objectives : These present study was designed to investigate the relaxation effects of Alpiniae Oxyphyllae Fructus(AOF) on isolated corpus cavernosum smooth muscle.Methods : Rabbit corpus cavernous tissues were prepared in strip. Then relaxation responses of AOF at 0.01-3 ㎎/㎖ in contracted strips induced by phenylephrine(PE) were measured. To evaluate mechanisms, indomethacin(IM) tetraethylammonium chloride(TEA), Nω-nitro-L-arginine(_L-NNA), methylene blue(MB) were treated before AOF extract(0.1-3 ㎎/㎖) infused into precontracted strips induced by PE. And 1 mM Ca²⁺was infused into precontracted strips after pretreatment of AOF extract(3 ㎎/㎖) in Ca²⁺-free krebs-ringer solution. NO concentration was measured by Griess reagent system. Ratio of smooth muscles to collagen fibers and eNOS positive reaction were measured by histocheminal and immunohistochemical process.Results : The cavernous strips were significantly relaxed by AOF extract 0.1, 0.3, 1, 3 ㎎/㎖ and the pretreatment with IM 10 μM,_L-NNA 100 μM, MB 10 μM inhibited relaxation of AOF compared to non-pretreatment, but the pretreatment with TEA 100 μM didn't affect relaxation of AOF. In a Ca²⁺-free solution, pretreatment with AOF reduced increase on contraction of strips by Ca²⁺supply than non-pretreatment. On HUVEC, NO concentration was increased. On corpus cavernosum of penis in Spontaneous Hypertensive Rat, ratio of smooth muscles to collagen fibers and eNOS positive reaction in AOF group were increased compared to PE groupConclusions : Taken this results, we can suggest that AOF extract exerts a relaxation effects on rabbit corpus cavernosum smooth muscle in part by suppressing influx of extracellular Ca²⁺throughout prostacyclin, the NO-cGMP system.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.28 no.8
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• pp.311-315
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• 2016

The efficiency of manure treatment was investigated in terms of the pH, BOD, COD, SS, T-N and TP with a variation in the injection amount of PAC, polymer and ozone. The wastewater flow rate to the AOF is of $7.4m^3/hr$ with a reaction time of 30 minutes. The amount of PAC and polymer changed by 30, 35, 40 ml/min, and 30, 40, 50 ml/min, respectively. The amount of ozone injected varied from 110, 125, and $150kg-O_3/hr$. The optimum manure treatment performance was found for a PAC of 35 ml/min for the COD and SS, with polymer of 30 ml/min, and ozone injection of $150kg-O^3/hr$. A substantially optimum dose for each PAC, polymer, and ozone was also found to exist.