Processing math: 100%
  • Title/Summary/Keyword: ANTIBACTERIAL ACTIVITY

Search Result 2,224, Processing Time 0.035 seconds

Entomological approach to the impact of ionophore-feed additives on greenhouse gas emissions from pasture land in cattle

  • Takahashi, Junichi;Iwasa, Mitsuhiro
    • Journal of Animal Science and Technology
    • /
    • v.63 no.1
    • /
    • pp.16-24
    • /
    • 2021
  • The suppressive effect of monensin as an ionophore-feed additive on enteric methane (CH4) emission and renewable methanogenesis were evaluated. To clarify the suppressive effect of monensin a respiratory trial with head cage was performed using Holstein-Friesian steers. Steers were offered high concentrate diets (80% concentrate and 20% hay) ad libitum with or without monensin, galacto-oligosaccharides (GOS) or L-cysteine. Steers that received monensin containing diet had significantly (p < 0.01) lower enteric CH4 emissions as well as those that received GOS containing diet (p < 0.05) compared to steers fed control diets. Thermophilic digesters at 55℃ that received manure from steers fed on monensin diets had a delay in the initial CH4 production. Monensin is a strong inhibitor of enteric methanogenesis, but has a negative impact on biogas energy production at short retention times. Effects of the activity of coprophagous insects on CH4 and nitrous oxide (N2O) emissions from cattle dung pats were assessed in anaerobic in vitro continuous gas quantification system modified to aerobic quantification device. The CH4 emission from dungs with adults of Caccobius jessoensis Harold (dung beetle) and the larvae of the fly Neomyia cornicina (Fabricius) were compared with that from control dung without insect. The cumulative CH4 emission rate from dung with dung insects decreased at 42.2% in dung beetles and 77.8% in fly larvae compared to that from control dung without insects. However, the cumulative N2O emission rate increased 23.4% in dung beetles even though it reduced 88.6% in fly larvae compared to dung without coprophagous insects. It was suggested that the antibacterial efficacy of ionophores supplemented as a growth promoter still continued even in the digested slurry, consequently, possible environmental contamination with the antibiotics might be active to put the negative impact to land ecosystem involved in greenhouse gas mitigation when the digested slurry was applied to the fields as liquid manure.

The Clinical Effects of Platycodon grandiflorum: A Systematic Review (도라지(Platycodon grandiflorum)의 임상적 효과에 대한 체계적 문헌 고찰)

  • Jung, Cheol Woon;Kim, Sun Jo;Anh, Nguyen Hoang;Lee, Eun Goo;Kim, Tae Ha;Kwon, Sung Won;Yang, Hyun Ok;Lee, Seul Ji
    • Korean Journal of Pharmacognosy
    • /
    • v.52 no.1
    • /
    • pp.1-12
    • /
    • 2021
  • Platycodon (Platycodon grandiflorum) is used as a food and medicinal ingredient worldwide, particularly in Northeast Asia. Although a large number of studies have been conducted to determine its medicinal efficacy, there is still a paucity of literature summarizing the clinical activity of Platycodon. In this systematic review, we aimed to summarize the clinical efficacy of Platycodon. All literature describing the clinical efficacy of Platycodon was collected from international databases, and relevant papers were carefully evaluated for eligibility. Data were extracted from the selected papers and quality evaluation was conducted in accordance with the standards provided by the National Institutes of Health (NIH), with all procedures being conducted in accordance with PRISMA 2009 checklist guidelines. After the removal of 342 papers, 644 studies were fully screened for their titles and abstracts, among which, 19 studies were finally selected for full summarization. Extraction of data indicated evidence of the effectiveness of Platycodon in the treatment of respiratory system-related diseases and cancer, and in the provision of immunity. However, we found that the quality and objectivity of evaluation, management of variable factors, sample size, and reproducibility were generally poor. Although Platycodon has shown potential antiviral, antibacterial, anticancer, and antidepressant effects, and promotion of immunity in clinical settings, it was not possible to integrate objective indicators to conduct a meta-analysis due to the lack of literature and insufficient studies. More investigations in the clinical setting should be conducted to confirm the functional effects of Platycodon.

Analysis of Aroma Components by Part of Allium Hookeri and Research on Antioxidant and Anti-inflammatory (삼채잎 향기 성분 분석과 항산화 및 항염 효과 연구)

  • Shin, Min Chul;Jeong, Sook Heui
    • Journal of the Korean Applied Science and Technology
    • /
    • v.38 no.1
    • /
    • pp.263-270
    • /
    • 2021
  • Allium Hookeri is a plant of the genus Allium, and prior research has been conducted on antioxidant, anti-inflammatory and antibacterial, but studies on essential oil extraction are insufficient. Therefore, in this study, GC-MSD was performed by extracting essential oils for Allium Hookeri leaves, roots, and whole parts. The cytotoxicity, antioxidant, and anti-inflammatory properties of Allium Hookeri leaves were confirmed to confirm their potential as a cosmetic material. The yield of essential oil extraction from Allium Hookeri leaves, roots and whole parts was found to be 0.01, 0.02, 0.01%. As a result of analyzing the aroma components of GC-MSD, the main components of essential oil of Allium Hookeri leaves were Diallyl trisulfide (34.02%) and Methyl allyl trisulfide (25.14%). At a concentration of 10%, where cytotoxicity was not confirmed, 39.69% inhibition of NO production and 88.26% of DPPH radical scavenging activity were shown. Through this, the Allium Hookeri essential oil presents the possibility of being useful as a raw material with antioxidant and anti-inflammatory effects in cosmetics.

Determination of Semen Quality and Antibacterial Susceptibility Pattern of Bacteria Isolated from Semen of Iraqi Subjects

  • Faisal, Anwer Jaber;Salman, Hamzah Abdulrahman
    • Microbiology and Biotechnology Letters
    • /
    • v.49 no.4
    • /
    • pp.587-593
    • /
    • 2021
  • Infertility is a key issue affecting mood and behavior in men. Microorganisms are one of the primary etiological agents that may be associated with infertility. The objective of the present study was to identify bacterial causative agents from the semen of infertile subjects and determine the effect of bacterial infection on sperm quality, as well as determine the susceptibility of these bacteria to drugs. Forty semen samples from 30 infertile patients and 10 fertile individuals were collected. The pH, volume, motility, and concentration of semen were analyzed. The samples were processed and identified by biochemical testing using API identification kits. The antibiotic susceptibility pattern was determined using the disc diffusion method. Abnormal sperm quality was observed. The mean age of the individual and their sperm morphology, concentration, progressive motility, pH level, and pus cell content were 31.9 years, 2.7%, 10.4 million/ml, 27.3%, 8.3, and 5.7, respectively. Among the tested samples, oligoasthenozoospermia was found to show the highest occurrence, at 27/30 samples, followed by teratozoospermia, at 25/30 samples, and asthenozoospermia, at 22/30 samples. Of the tested infertile patients' sperm, 19, 6, and 5 isolates were identified as Escherichia coli, Klebsiella pneumonia, and Staphylococcus epidermidis, respectively. The results also revealed multi-drug resistance in the bacteria. Compared to that shown by the other tested antibiotics, amikacin showed higher activity against all isolated bacteria. However, the bacteria exhibited maximum resistance against gentamicin, cefotaxime, levofloxacin, and ampicillin. In conclusion, leukocytospermia and bacterial infections are possibly responsible for sperm abnormalities. Multi-drug resistant bacteria were detected. Gentamicin, cefotaxime, levofloxacin and ampicillin were shown the highest resistance, while amikacin was the most effective antimicrobial agent against the isolated bacteria.

Quality Monitoring of Specification Standard of Gardeniae Fructus in the Korean Pharmacopoeia and Studies HPLC Standard Chromatogram (치자(梔子)의 규격 기준 모니터링 및 HPLC 표준크로마토그램 연구)

  • Kim, Kyoung Hee;Kim, Sun Mi;Shin, Seung Hoon;Lee, Young Jong;Baek, Wan Sook
    • The Korea Journal of Herbology
    • /
    • v.32 no.2
    • /
    • pp.97-105
    • /
    • 2017
  • Objectives : Gardeniae Fructus is a ripe fruit of Gardenia jasminoides Ellis, which has been used as traditional medicines for anti-inflammatory, diuretic, antipyretic, and antibacterial activity. The aim of this study was to compare of Gardeniae Fructus in South Korea collected during three years according to the standards in monographs of the Korean Pharmacopoeia Eleventh edition (KP11). Methods : 30 items of Gardeniae Fructus from two cultivation regions were classified into dried(n=15) & steamed (n=15) and tested according to the standards in monographs of the KP11. Gardeniae Fructus was carried out identification(comparison of colors, thin layer chromatography), heavy metals, residual pesticides, total ash, and assay registered at KP11. Add to we tested loss on dry, contents of ethanol-soluble extracts, and HPLC profiling. Results : In TLC chromatogram of identification test, the spot of gardenoside and geniposide were observed at Rf value of about 0.3 and 0.5. Heavy metals and residual pesticides met the requirements of the standards for all samples. The results of total ash of each samples are measured maximum 4.87 %. According to HPLC for assay, the samples contain 4.80~6.10 % of geniposide and 0.45~1.83 % of gardenoside. Conclusion : We have verified the current specification standard of Gardeniae Fructus and standard that is not set. By the results, it is proposed a new draft of loss on drying and confirmed the content of gardenoside revised. HPLC standard chromatogram of Gardeniae Fructus is proposed. We hope that it will help the standardization of Gardeniae Fructus.

Optimal Processing for Peptic Hydrolysate from Flounder Skin and Its Skincare Function (광어껍질을 활용한 펩신가수분해물 제조공정 최적화와 피부건강 기능성)

  • Kang, You-an;Jin, Sang-Keun;Ko, Jonghyun;Choi, Yeung Joon
    • Journal of Marine Bioscience and Biotechnology
    • /
    • v.14 no.1
    • /
    • pp.9-24
    • /
    • 2022
  • Low-molecular weight peptides derived from fish collagen exhibit several bioactivities, including antioxidant, antiwrinkle, antimicrobial, antidiabetic, and antihypertension effects. These peptides are also involved in triglyceride suppression and memory improvement. This study aimed to investigate the optimal processing condition for preparing low-molecular weight peptides from flounder skin, and the properties of the hydrolysate. The optimal processing conditions for peptic hydrolysis were as follows: a ratio of pepsin to dried skin powder of 2% (w/w), pH of 2.0, and a temperature of 50℃. Peptic hydrolysate contains several low-molecular weight peptides below 300 Da. Gly-Pro-Hyp(GPHyp) peptide, a process control index, was detected only in peptic hydrolysate on matrix-assisted laser desorption/ionization-time-of-flight(MALDI-TOF) spectrum. 2,2'-azinobis-(3-3-ethylbenzothiazolline-6- sulfonic acid(ABTS) radical scavenging activity of the peptic hydrolysate was comparable to that of 1 mM ascorbic acid, which was used as a positive control at pH 5.5, whereas collagenase inhibition was five times higher with the peptic hydrolysate than with 1 mM ascorbic acid at pH 7.5. However, the tyrosinase inhibition ability of the peptic hydrolysate was lower than that of arbutin, which was used as a positive control. The antibacterial effect of the peptic hydrolysate against Propionibacterium acne was not observed. These results suggest that the peptic hydrolysate derived from a flounder skin is a promising antiwrinkle agent that can be used in various food and cosmetic products to prevent wrinkles caused by ultraviolet radiations.

How to develop strategies to use insects as animal feed: digestibility, functionality, safety, and regulation

  • Jae-Hoon, Lee;Tae-Kyung, Kim;Ji Yoon, Cha;Hae Won, Jang;Hae In, Yong;Yun-Sang, Choi
    • Journal of Animal Science and Technology
    • /
    • v.64 no.3
    • /
    • pp.409-431
    • /
    • 2022
  • Various insects have emerged as novel feed resources due to their economical, eco-friendly, and nutritive characteristics. Fish, poultry, and pigs are livestock that can feed on insects. The digestibility of insect-containing meals were presented by the species, life stage, nutritional component, and processing methods. Several studies have shown a reduced apparent digestibility coefficient (ADC) when insects were supplied as a replacement for commercial meals related to chitin. Although the expression of chitinase mRNA was present in several livestock, indigestible components in insects, such as chitin or fiber, could be a reason for the reduced ADC. However, various components can positively affect livestock health. Although the bio-functional properties of these components have been verified in vitro, they show positive health-promoting effects owing to their functional expression when directly applied to animal diets. Changes in the intestinal microbiota of animals, enhancement of immunity, and enhancement of antibacterial activity were confirmed as positive effects that can be obtained through insect diets. However, there are some issues with the safety of insects as feed. To increase the utility of insects as feed, microbial hazards, chemical hazards, and allergens should be regulated. The European Union, North America, East Asia, Australia, and Nigeria have established regulations regarding insect feed, which could enhance the utility of insects as novel feed resources for the future.

Antibacterial Activity of Citrus junos Pulp according to Breeding Method (유자의 번식방법에 따른 유자 과육의 항균활성)

  • Ji-Won Seo;Shi-Qi Wang;Ma-ru Im;Da-Kyung Choi;Won-Seob Song
    • Proceedings of the Plant Resources Society of Korea Conference
    • /
    • 2023.04a
    • /
    • pp.70-70
    • /
    • 2023
  • 유자의 번식방법은 세 가지로 알려져 있다. 유자 대목의 유자를 접목하는 방법과 탱자 대목의 유자를 접목하는 방법, 유자의 종자를 파종하여 유자나무를 번식하는 방법 등이 있다. 이러한 번식 방법 가운데 가장 많이 활용되고 있는 유자의 번식 방법은 탱자 나무 대목에 유자를 접목하여 생산하는 방법이다. 실제로 유자의 생산물을 비교해보면 과일의 크기는 유자나무에 유자를 접목시킨 유자나무에서 대체적으로 과일이 크고 색깔도 진노랑색이다. 과일이 제일 작은 번식 방법은 유자 종자를 파종하여 유자를 생산하는 방법이다. 또한, 유자 접목 방법에 따른 유자의 생산 연령은 실생번식보다 접목방법이 훨씬 빠르다. 즉, 과일의 형태와 색깔, 향 등이 번식 방법에 따라서 다소 차이를 나타내고 있다. 따라서, 이러한 번식 방법에 따른 유자의 과육으로부터 항균 활성에 차이를 분석하고자 본 실험을 실시하였다. 3종의 항균실험을 실시한 결과 대부분의 균 종류의 결과를 보면 실생 번식에서 다소 양호한 항균활성을 나타내었다. 유자 대목의 유자를 접목한 유자의 과육도 비교적 항균활성이 좋게 나타났다. 탱자 대목에 유자를 접목하여 얻어진 유자 과육의 항균 활성은 다른 번식 방법에 비하여 다소 미흡한 항균 활성 결과를 나타내었다. 따라서, 이러한 결과들로 미루어볼 때 번식 방법에 따른 유자 과육의 항균 활성은 균 종류별 차이가 나는 것으로 판단되었다. 따라서, 이러한 결과들로 미루어 볼 때 향후 과피의 항균 활성을 조사한다면 더 좋은 결과를 확보할 수 있을 것으로 생각된다

  • PDF

Combinational effects of sesquiterpene lactones isolated from bay laurel (Laurus nobilis) leaves with antibiotics against fish pathogenic bacteria (어류 병원성 세균에 대한 월계수(Laurus nobilis) 잎 유래 sesquiterpene lactone과 수산용 항생제의 병용효과)

  • Jae-Woong Lim;Ji-Seok Choi;Ayman Turk;Mi Kyeong Lee;Do-Hyung Kim;So Young Kang
    • Journal of fish pathology
    • /
    • v.37 no.1
    • /
    • pp.133-138
    • /
    • 2024
  • This study is a report on combinational effects between four sesquiterpene lactones (SLs) from bay laurel (Laurus nobilis) leaves, and oxytetracycline (OTC) or amoxicillin (AMX) against four fish pathogenic bacteria such as Vibrio anguillarum, V. harveyi, Edwardsiella tarda, and Streptococcus iniae. Individually, four SLs exerted little antibacterial activity against fish pathogenic bacteria. However, when combined with OTC or AMX, they showed synergistic interaction against pathogenic bacteria. Especailly, zaluzanin C (1) reduced the MIC of OTC (or AMX) eight-fold. Our results showed that combinations of SLs with antibiotics (ABTs) are more effective than ABTs alone to control pathogenic bacteria. The highest synergistic effect was observed when zaluzanin C (1) was combined with OTC or AMX against V. harvey or S. iniae, displaying significant reductions of MICs up to 8-fold (0.125 to 0.015 ㎍/mL and 0.0078 to 0.0009 ㎍/mL). In addition, zaluzanin C (1) improved the antibiotic potency of OTC against OTC resistant V. harveyi (250 ㎍/mL to 62.5 ㎍/mL). Synergism between ABTs and phytochemical such as SLs could be a therapeutically helpful concept to improve the efficacy of ABTs and prevent antibiotic resistance. These results suggest that SLs can be used as an alternative to reduce antibiotic resistance in aquaculture.

Cathelicidin-related Antimicrobial Peptide Contributes to Host Immune Responses Against Pulmonary Infection with Acinetobacter baumannii in Mice

  • Min-Jung Kang;Ah-Ra Jang;Ji-Yeon Park;Jae-Hun Ahn;Tae-Sung Lee;Dong-Yeon Kim;Do-Hyeon Jung;Eun-Jung Song;Jung Joo Hong;Jong-Hwan Park
    • IMMUNE NETWORK
    • /
    • v.20 no.3
    • /
    • pp.25.1-25.13
    • /
    • 2020
  • Acinetobacter baumannii is known for its multidrug antibiotic resistance. New approaches to treating drug-resistant bacterial infections are urgently required. Cathelicidin-related antimicrobial peptide (CRAMP) is a murine antimicrobial peptide that exerts diverse immune functions, including both direct bacterial cell killing and immunomodulatory effects. In this study, we sought to identify the role of CRAMP in the host immune response to multidrug-resistant Acinetobacter baumannii. Wild-type (WT) and CRAMP knockout mice were infected intranasally with the bacteria. CRAMP-/- mice exhibited increased bacterial colony-forming units (CFUs) in bronchoalveolar lavage (BAL) fluid after A. baumannii infection compared to WT mice. The loss of CRAMP expression resulted in a significant decrease in the recruitment of immune cells, primarily neutrophils. The levels of IL-6 and CXCL1 were lower, whereas the levels of IL-10 were significantly higher in the BAL fluid of CRAMP-/- mice compared to WT mice 1 day after infection. In an in vitro assay using thioglycollate-induced peritoneal neutrophils, the ability of bacterial phagocytosis and killing was impaired in CRAMP-/- neutrophils compared to the WT cells. CRAMP was also essential for the production of cytokines and chemokines in response to A. baumannii in neutrophils. In addition, the A. baumannii-induced inhibitor of κB-α degradation and phosphorylation of p38 MAPK were impaired in CRAMP-/- neutrophils, whereas ERK and JNK phosphorylation was upregulated. Our results indicate that CRAMP plays an important role in the host defense against pulmonary infection with A. baumannii by promoting the antibacterial activity of neutrophils and regulating the innate immune responses.