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  • Title/Summary/Keyword: ANTIBACTERIAL ACTIVITY

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Natural Extracts as Inhibitors of Microorganisms and Lipid Oxidation in Emulsion Sausage during Storage

  • Lee, Jeeyeon;Sung, Jung-Min;Cho, Hyun Jin;Woo, Seung-Hye;Kang, Min-Cheol;Yong, Hae In;Kim, Tae-Kyung;Lee, Heeyoung;Choi, Yun-Sang
    • Food Science of Animal Resources
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    • v.41 no.6
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    • pp.1060-1077
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    • 2021
  • Food additives are required to maintain the freshness and quality of foods, particularly meats. However, chemical additives may not be preferred by consumers, and natural materials with antimicrobial and antioxidant effects may be used as replacements for common chemical additives. Accordingly, in this study, we compared the antimicrobial and antioxidant activities of natural compounds extracted with ethanol and hot water, and emulsion sausage prepared with natural ethanol extracts was analyzed for pH, color, thiobarbituric acid reactive substances (TBARS), and Clostridium perfringens growth during storage. The antimicrobial activities of 49 natural extract candidates against Listeria monocytogenes, C. perfringens, Salmonella spp., and Escherichia coli were analyzed, and six natural materials with excellent antibacterial activities, i.e., Elaeagnus umbellata Thunb. f. nakaiana (Araki) H. Ohba, Punica granatum L., Ecklonia cava, Nelumbo nucifera Gaertner, and Schisandra chinensis (Turcz.) Baill., and Rubus coreanus Miq. were evaluated to determine their total polyphenol contents and DPPH radical scavenging activities. The total polyphenol contents of ethanol extracts were higher than those of hot water extracts, whereas DPPH radical scavenging activity was found to be higher in hot water extracts. The TBARS values of emulsion sausages were significantly increased as storage time increased, and the TBARS values of emulsion sausages prepared with natural extracts were lower than those of control sausages. Natural extract-treated emulsion sausages showed a 99% reduction in bacterial contents compared with untreated sausages on day 2, with greater than 99.9% reduction after day 3. Thus, these results demonstrated that natural extracts could have applications as natural preservatives in meat products.

Efficacy of Lactobacillus fermentum Isolated from the Vagina of a Healthy Woman against Carbapenem-Resistant Klebsiella Infections In Vivo

  • Tajdozian, Hanieh;Seo, Hoonhee;Kim, Sukyung;Rahim, Md Abdur;Lee, Saebim;Song, Ho-Yeon
    • Journal of Microbiology and Biotechnology
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    • v.31 no.10
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    • pp.1383-1392
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    • 2021
  • Carbapenem-resistant Enterobacteriaceae (CRE) that produce Klebsiella pneumoniae carbapenemase are increasingly reported worldwide and have become more and more resistant to nearly all antibiotics during the past decade. The emergence of K. pneumoniae strains with decreased susceptibility to carbapenems, which are used as a last resort treatment option, is a significant threat to hospitalized patients worldwide as K. pneumoniae infection is responsible for a high mortality rate in the elderly and immunodeficient individuals. This study used Lactobacillus fermentum as a candidate probiotic for treating CRE-related infections and investigated its effectiveness. We treated mice with L. fermentum originating from the vaginal fluid of a healthy Korean woman and evaluated the Lactobacilli's efficacy in preventive, treatment, nonestablishment, and colonization mouse model experiments. Compared to the control, pre-treatment with L. fermentum significantly reduced body weight loss in the mouse models, and all mice survived until the end of the study. The oral administration of L. fermentum after carbapenem-resistant Klebsiella (CRK) infection decreased mortality and illness severity during a 2-week observation period and showed that it affects other strains of CRK bacteria. Also, the number of Klebsiella bacteria was decreased to below 5.5 log10 CFU/ml following oral administration of L. fermentum in the colonization model. These findings demonstrate L. fermentum's antibacterial activity and its potential to treat CRE infection in the future.

Quality and Sensory Characteristics of Jinmal Dasik Using Pine Needle Powder (잣잎분말을 이용한 진말다식의 품질 및 감각 특성)

  • Kim, Yoonji;Park, Eunbin;Ryu, Soo In;Lee, Minho;Lee, Hyo-Jeong;Kang, Ahyoung;Paik, Jean Kyung
    • The Korean Journal of Food And Nutrition
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    • v.34 no.5
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    • pp.498-505
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    • 2021
  • This study tried to develop Jinmal dasik using pine nuts leaf powder with antibacterial and antioxidant properties. After preparing the Jinmal dasik in proportions of 0%, 2%, 4%, 6%, and 8% by varying the ratio of pine needle powder, the moisture, color, physical properties, antioxidant properties and sensory quality were measured. Except for the 8% Jinmal dasik, the L value of chromaticity significantly decreased as the amount of pine needle powder increased (p<0.001). In addition, the value significantly decreased with increased density (p<0.001). Based on our physical properties evaluation results, the hardness, cohesiveness, and elasticity decreased significantly as the amount of pine needle powder increased (p<0.001), but adhesion and chewiness increased significantly with an increase in the amount of pine needle powder (p<0.001). Total polyphenol content was significantly increased except for the 8% Jinmal dasik (p<0.001). DPPH and ABTS also increased significantly as the amount of pine needle powder increased (p<0.001). Our sensory evaluation, showed the 2% Jinmal dasik to have the highest degree of preference, however, there was no significant difference among the proportion groups (p=0.053).

Evaluation of the wound healing ability of an Abeliophyllum distichum Nakai extract in ICR mouse and of antibacterial activity against human cutaneous flora

  • Go, Young Bin;Lee, Ji Hwan;Oh, Han Jin;Kim, Yong Ju;An, Jae Woo;Chang, Se Yeon;Song, Dong Cheol;Cho, Hyun Ah;Cho, Jin Ho
    • Korean Journal of Agricultural Science
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    • v.48 no.3
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    • pp.567-574
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    • 2021
  • In this study, we evaluated the wound healing rate and, inflammatory cells effects of by Abeliophyllum distichum Nakai (ADN) extract in mice. We also assessed the stability of the ADN extract upon exposure to sunlight. Treatments were as follows: 1) CON (only saline solution), T1 (CON + 0.0125% ADN extract), T2 (CON + 0.05% ADN extract), and T3 (CON + 0.5% ADN extract). A 4 mm punch was used in the central part of the dorsal area to separate it from the subcutaneous tissue, causing a full-thickness skin wound. An amount of 1 mL of each sample was sprayed onto the treatment section of the wound with a pipette every day from the day of wound creation, with proper application ensured using brush. In the stability test, the pH was measured at 1, 4, and 8 weeks after exposing the samples of each treatment section to sunlight considering, the higher concentrations of the ADN extract. The results of this study indicate that the effectiveness of the wound contraction rate in the mice to which the ADN extract was applied was low. Moreover, the stability of the sample containing a high concentration of the ADN extract could not be verified. In addition, no significant results were obtained in the inflammatory reaction assessment. Therefore, additional research focusing on wound contraction, stability, and inflammatory cell outcomes of the ADN extract is needed.

Inhibitory effect of n-hexane extract from Korean red ginseng marc against Streptococcus mutans causing dental caries (홍삼박 n-hexane 추출물의 충치를 유발하는 Streptococcus mutans 저해 효과)

  • Kim, Dong Chung;In, Man-Jin
    • Journal of Applied Biological Chemistry
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    • v.64 no.4
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    • pp.357-362
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    • 2021
  • In this study, to investigate the anticariogenic effect of red ginseng, the antibacterial effect, cell adherence and biofilm formation inhibitory effect of n-hexane extract of red ginseng marc (HERGM) against Streptococcus mutans, the causative bacteria of caries, were measured. The growth of S. mutans was inhibited in proportion to the concentration of HERGM, and was hardly observed at a concentration above 125 ㎍/mL (MIC =125 ㎍/mL). It was found that HERGM acts on the cell membrane and the nucleic acid component of the cell was leaked. In addition, HERGM inhibited the adherence and biofilm formation of S. mutans by more than 90% at a concentration of 125 ㎍/mL. GTase activity was completely inhibited at a concentration of 50 ㎍/mL of HERGM. In conclusion, it was found that HERGM commonly inhibited the growth and biofilm formation of S. mutans.

Influence of soymilk and skim milk on growth and antibacterial activity of lactic acid bacteria (유산균의 증식과 항균 활성에 관한 탈지유 및 두유의 영향)

  • Lim, Eun-Seo
    • Korean Journal of Microbiology
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    • v.55 no.3
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    • pp.258-267
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    • 2019
  • The purpose of this study was to investigate the effect of lactic acid bacteria (LAB) on the growth and biogenic amines (BA) formation of Enterobacter aerogenes CIH05 in skim milk and soymilk. Lactobacillus acidophilus GK20, Lactobacillus paracasei GK74, and Lactobacillus plantarum GK81 isolated from mustard kimchi did not produce BA in the decarboxylation broth. L. paracasei GK74 exhibited the highest cell viability and antimicrobial compounds producing ability in fermented skim milk and soymilk samples, while the lowest producer was L. plantarum GK81. The production yield of lactic acid, hydrogen peroxide, and bacteriocin was dependent on the species of Lactobacillus and the type of culture medium. As LAB the number of viable cells of E. aerogenes CIH05 were higher in skim milk than in soymilk. When mixed culture with L. acidophilus GK20 and L. paracasei GK74 and treated with bacteriocin solution (300 AU/ml) obtained from these strains in milk media, the cell growth and cadaverine and histamine contents of E. aerogenes CIH05 were significantly (P < 0.05) lower than the respective values in control sample.

Screening of Endophytic Antagonistic Bacterium from Phellodendron amurense and Their Biocontrol Effects against Canker Rot

  • Li, Shujiang;Fang, Xinmei;Zhang, Hanlian;Zeng, Yanling;Zhu, Tianhui
    • The Plant Pathology Journal
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    • v.35 no.3
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    • pp.234-242
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    • 2019
  • Thirty-four strains of bacteria were isolated from Phellodendron amurense. Using Nectria haematococca as an indicator strain, the best strain, B18, was obtained by the growth rate method. The morphological, physiological and biochemical characteristics of strain B18 and its 16S DNA gene sequence were identified, and the biocontrol effect of strain B18 was assessed in pot and field tests, as well as in a field-control test. Drilling methods were used to determine the antibacterial activity of metabolites from strain B18 and their effects on the growth of pathogen mycelia and spores. The best bacteriostatic rate was 85.4%. B18 can hydrolyse starch and oxidize glucose but does not produce gas; a positive result was obtained in a gelatine liquefaction test. According to 16S DNA gene sequencing, strain B18 is Bacillus methylotrophicus (GenBank accession number: MG457759). The results of pot and field-control trials showed 98% disease control when inoculating 108cfu/ml of the strain. The disease control effect of the B18 culture liquid (concentrations of 108, 2×106, 106, 5×105 and 2.5×105cfu/ml) in the field-control test was higher than 80%, and the cure rate of the original delivery solution was 96%. Therefore, in the practical forestry production, a 2.5×105cfu/ml culture liquidshould be applied in advance to achieve good control effects.

Bioactive Compounds Isolated from Pinus densiflora and koraiensis and their Applications (Pinus densiflora와 Pinus koraiensis로부터 분리된 생리활성 물질들 및 응용)

  • Kim, Nam Hee;Yoon, Sunkwon;Um, Byung-Hun;Kim, Jung Won
    • Applied Chemistry for Engineering
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    • v.32 no.2
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    • pp.132-138
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    • 2021
  • This review investigated the types of physiologically active substances isolated and extracted from Pinus densiflora and koraiensis native to Republic of Korea, and the current status of research on their effectiveness and industrial use. They contain various bioactive substances including essential oils, polyphenols, resins, and stilbene derivatives. In recent, physiological activities such as antioxidant, anti-inflammatory, antibacterial, cataract prevention, and neuroprotection from extracts of Pinus densiflora and koraiensis have been identified by domestic researchers. The extracts have been used in different industrial fields like food, health functional foods, cosmetics, and household goods, but the high proportion of them is industrially made from exotic species. Therefore, various studies on industrial applicability are needed due to the lack of cases in which the activity is applied to actual products, with respect to the effects that have been scientifically recognized.

Effects of Medicinal Herb Extracts of Artemisia iwayomogi Kitamura and Angelica gigas Nakai on Disease Resistance in Olive Flounder Paralichthys olivaceus (한인진(Artemisia iwayomogi Kitamura)과 참당귀(Angelica gigas Nakai) 추출물이 넙치(Paralichthys olivaceus) 항병력에 미치는 영향)

  • Kim, Na Young;Lee, Nam-Sil;Jeon, Eun Ji;Seo, Jung Soo;Woo, Soo Ji;Kim, Myung Sug;Kang, So Young;Jung, Sung Hee
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.5
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    • pp.634-643
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    • 2021
  • Han In-jin (Artemisia iwayomogi Kitamura) and Cham Dang-gwi (Angelica gigas Nakai) exhibit antibacterial, antiparasitic, antifungal, and antiviral properties in vitro. In this study, mixture of the extracts of these two medicinal plants was absorbed on pellets. Thereafter, these pellets were fed to olive flounder Paralichthys olivaceus for 12 weeks at laboratory (1st experiment) and 24 weeks at field test (2nd experiment), and the immune activity and disease resistance properties of the extracts were examined. It was observed that lysozyme activities of plasma, spleen, and kidney improved after 12 weeks. Furthermore, when the olive flounders were artificially infected with bacterial pathogens, their cumulative mortality decreased in the group that was fed the extracts for 12 weeks compared to that in control group, and the relative percent survival also improved. This study concluded that mixture of Han In-jin and Cham Dang-gwi extracts provides disease resistance in vivo.

Effects of Cosmetics containing Pycnogenol on the skin of Korean Women in their 40s and 50s - Skin Clinical Approach (피크노제놀을 함유한 화장품이 40~50대 한국 여성의 피부에 미치는 영향 - 피부임상학적 접근)

  • Kim, Kyung-Yun;Ku, Jung-Eun
    • Journal of the Korea Convergence Society
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    • v.12 no.8
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    • pp.309-315
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    • 2021
  • Pycnogenol extracted from pine bark is a component with great antibacterial activity and antioxidant effect. It is applied as a natural anti-inflammatory agent with various medical effects including anti-inflammatory effects, regulation of blood pressure, regulation of the immune system, and inhibition of cancer cell growth. However, research related to cosmetics is limited. Therefore, in this study, the effect of Pycnogenol on the skin was studied through a clinical approach. Changes in skin condition were observed after using cosmetics with Pycnogenol and without Pycnogenol for 6 weeks for 10 clinicians in each group. We observed the effect of pore reduction, wrinkle reduction around eyes, a decrease of the number and angle of loose pores, and reduction of pigmentation. Therefore, cosmetics containing Pycnogenol have the effect of improving skin problems of aging skin.