• 대한핵의학회지
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• 제39권2호
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• pp.94-99
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• 2005

Electrocardiogram-gated single photon omission computed tomography (SPECT) provides valuable information in the assessment of both myocardial perfusion and ventricular function. Tl-201 is a suboptimal isotope for gating. Tl-201 images are more blurred compared with Tc-99m tracers due to the increased amount of scattered photons and use of a smooth filter. The average myocardial count densities are approximately one-half those of conventional technetium tracers. However, Tl-201 is still widely used because of its well-established utility for assessing myocardial perfusion, viability and risk stratification. Gated SPECT with Tl-201 enables us to assess both post-stress and rest left ventricular volume and function. Previous studies with gated Tl-201 SPECT measurements of ejection fraction (EF), end-diastolic volume (EDV), end-systolic volume (ESV) have shown high correlation with first-pass radionuclide angiography, gated blood pool scan, Tc-99m-MIBI gated SPECT, contrast ventriculography, echocardiography, and 3-dimensional magnetic resonance imaging. However, problems related to these studies include few agreement data of EDV and ESV, use of a reference method that is likely to have the same systemic errors (gated Tc-99m-MIBI SPECT), and other technical factors related to the count density of gated SPECT. With optimization of gated imaging protocols and more validation studies, gated Tl-201 SPECT would be an accurate method to provide perfusion and function information in patients with coronary artery disease.

• 대한임상검사과학회지
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• 제41권1호
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• pp.42-46
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• 2009

Unlike most bacteria, Treponema pallidum subspecies cannot be readily isolated or sustained in cell culture for numerous generations. In korea, two non treponemal tests are currently considered as standard; the VDRL slide test and RPR card test. These tests are based on an antigen composed of an alcoholic solution containing measured amount of cardiolipin, cholesterol, and sufficient purified lecithin to produce reactivity. The nontreponemal reagin tests measure immunoglobulin M (IgM) and IgG to lipoidal material released from damaged host cells as well as to lipoprotein-like material and possibly by cardiolipin released from the treponemes. The object of the evaluation was to evaluate the performance of the Mediace RPR kit on the automated biochemistry analyzer system as a method for screen method of syphilis as well as to identify BFP possibility. For evaluation of routine screening test, a total 2,380 specimens tested by Mediace RPR from 28th Oct, 2007 to 22th Feb, 2008. For evaluation of BFP possiblility, we measured samples which have potential BFP reaction in Syphilis test such as ANA (anti-nuclear antibody) positive (135 samples), CRP (C-reactive protein) positive (100 samples), RF (Rheumatoid factor) positive (26 samples), and other potential BFP cases (17 samples) including total 278 samples. These samples were tested quantitative test Mediace RPR with Hitachi 7600 P module. For comparison with current manual test, VDRL slide test were performed. Of these 2380 specimens, 2350 were negative, 30 were positive, and one were positive with TPHA. Both methods agreed for 2356 (98.9%) samples. Of the 30 samples showed positive results over 1.0 R.U, 6 samples showed positive results with VDRL test. Of these 6 samples, 1 samples showed positive with TPHA test. The combination of the Automated Biochemistry analyzer and VDRL test for retest can be increase efficiency of syphilis screening test.

• Bulletin of the Korean Chemical Society
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• 제26권2호
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• pp.285-291
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• 2005

The nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) is the viral RNA-dependent RNA polymerase (RdRp), which is the essential catalytic enzyme for the viral replication and is an appealing target for the development of new therapeutic agents against HCV infection. A small amount of serum from a single patient with hepatitis C was used to get the genome of a Korean HCV isolate. Sequence analysis of NS5B 1701 nucleotides showed the genotype of a Korean isolate to be subtype 1b. The soluble recombinant HCV NS5B polymerase lacking the C-terminal 24 amino acids was expressed and purified to homogeneity. With the highly purified NS5B protein, we established in vitro systems for RdRp activity to identify potential polymerase inhibitors. The rhodanine family compounds were found to be potent and specific inhibitors of NS5B from high throughput screening (HTS) assay utilizing the scintillation proximity assay (SPA) system. The binding mode of an inhibitor was analyzed by measuring various kinetic parameters. Lineweaver-Burk plots of the inhibitor suggested it binds not to the active site of NS5B polymerase, but to an allosteric site of the enzyme. The activity of NS5B in in vitro polymerase reactions with homopolymeric RNA requires interaction with multiple substrates that include a template/primer and ribonucleotide triphosphate. Steady-state kinetic parameter, such as Km, was determined for the ribonucleotide triphosphate. One of compounds found interacts directly with the viral polymerase and inhibits RNA synthesis in a manner noncompetitively with respect to UTP. Furthermore, we also investigated the ability of the compound to inhibit NS5B-directed viral RNA replication using the Huh7 cell-based HCV replicon system. The investigation is potentially very useful for the utility of such compounds as anti-hepatitic agents.

• 한국조경학회지
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• 제27권1호
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• pp.79-89
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• 1999

Recent expansion of urban parks has been partly successful in terms of providing urban outdoor recreation space. Unfortunately we paid very little attention to the importance of providing regional identity to urban parks until recently. Thus one of the most important tasks of the contemporary landscape architects of Korea is to investigate ways to improve regional identity of urban parks. This study is aimed at evaluating how well the current urban parks have realized their own regional identity. To test the effectiveness of the concept of 'identity', the identity items were created, while a research model was developed by using such variables as in urban park identity, park satisfaction, belongingness to the region, and residential satisfaction. In order to test the model, the causations were analyzed. Meanwhile, the scales to evaluating identity were reviewed by means of the unstructures group interview, and were finally determined as 10 items; culture, uniqueness, symbolic, historic nature, homogeneity, harmony, difference, public benefit, nativeness and traditionality. Data from three new towns in the capital region-Bundang, Ilsan and Sanbon-were collected, and their central parks were referred to a questionnaire survey. The data were processed using descriptive statistics, correlation analysis, multiple regression analysis and path analysis. It was found through this survey that subjects 'sentiments regarding the sample towns' regional identity differed slightly. Residents of Bundang felt the strongest amount of regional identity, followed by those of Ilsan and Sanbon. In addition, the most important scale on regional identity is 'park satisfaction', followed by 'belongingness to the region' and 'residental satisfaction'. The path analysis was conducted to interpret the causations in a more detailed and comprehensive way than correlation analysis. As a result, it was proven that the regional identity affects 'belingingness to region' through the intermediated variable 'park satisfaction', while affecting ' residential satisfaction' through the intermediate variables 'park satisfaction' and 'beligningness to region'. In other words, although the regional identity of a park does not directly affect 'belongingness to region' or 'residental satisfaction', it has indirect effects on them through intermediate variables.

• International Journal of Naval Architecture and Ocean Engineering
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• 제6권2호
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• pp.471-483
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• 2014

Since 2009 of global financial crisis, shipbuilding industry has undergone hard times seriously. After such a long depression, the latest global shipping market index shows that the economic recovery of global shipbuilding market is underway. Especially, nations with enormous resources are going to increase their productivity or expanding their shipyards to accommodate a large amount of orders expected in the near future. However, few commercial projects have been carried out for the practical shipyard layout designs even though those can be good commercial opportunities for shipbuilding engineers. Shipbuilding starts with a shipyard construction with a large scale investment initially. Shipyard design and the equipment layout problem, which is directly linked to the productivity of ship production, is an important issue in the production planning of mass production of ships. In many cases, shipbuilding yard design has relied on the experience of the internal engineer, resulting in sporadic and poorly organized processes. Consequently, economic losses and the trial and error involved in such a design process are inevitable problems. The starting point of shipyard construction is to design a shipyard layout. Four kinds of engineering parts required for the shipyard layout design and construction. Those are civil engineering, building engineering, utility engineering and production layout engineering. Among these parts, production layout engineering is most important because its result is used as a foundation of the other engineering parts, and also, determines the shipyard capacity in the shipyard lifecycle. In this paper, the background of shipbuilding industry is explained in terms of engineering works for the recognition of the macro trend. Nextly, preliminary design methods and related case study is introduced briefly by referencing the previous research. Lastly, the designed work of layout design is validated using the computer simulation technology.

• 한국결정성장학회지
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• 제26권6호
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• pp.232-237
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• 2016

약물의 공결정 형성은 결정구조를 디자인하여 물리적인 성질을 제어하는 방법으로 각광받고 있다. 특히, 밀링 결정화법은 약물 공결정의 형성 여부를 판단하기 위한 스크리닝 기법으로 널리 사용되고 있는데, 대부분의 경우에 소량의 액체를 첨가하는 것이 필요하다. 본 연구에서는 액체의 첨가가 없는 밀링법을 가능하게 하기 위해서, ibuprofen/nicotinamide의 공결정화에 온도가 미치는 영향을 살폈다. 액체질소 냉각 온도와 상온에서의 밀링을 비교하였을 때, 액체질소 냉각 상태에서 공결정화가 상대적으로 효과적으로 진행된 것을 XRD, DSC 등의 분석법을 이용하여 확인하였다. 이러한 결과는 원료 물질의 유리전이 온도 이하에서 밀링하였을 때 분자의 운동성이 제한되어, 원료 결정구조의 파쇄와 공결정 유도가 효과적으로 일어나는 것으로 해석할 수 있다. 본 결과가 약물 밀링 공결정화에 응용되기 위해서는 여러 약물 공결정에 대한 추가 연구가 필요하다.

• 설비공학논문집
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• 제27권3호
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• pp.128-136
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• 2015

Reducing the building energy consumption has become one of the most important issues. However, the current engineering and technological involvement in energy analysis has been relatively low in the existing buildings. In the existing buildings, end-use analysis must be accompanied to calculate the exact amount in energy savings and such analysis should be conducted based on the energy billing data or measurement data by calibration process. Mostly, detailed energy simulation programs have been proposed for the analysis but, it is difficult to utilize them due to realistic problems. In this paper, we developed an end-use analysis tool that have input function for energy audit data and two case studies were conducted in the real-life office buildings located in Seoul, Korea. Mean Bias Error (MBE) and Coefficient of Variation of Root-Mean- Squreaed-Error (CV(RMSE)) are used for the criteria of comparison. Each index was calculated by using monthly utility bills of electricity and gas consumption. Results showed that MBE and CV (RMSE) represented with acceptable values of -0.1% and 5.7% respectively.

• 한국방송∙미디어공학회:학술대회논문집
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• 한국방송공학회 2009년도 IWAIT
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• pp.709-713
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• 2009

Recently, a lot of research that applies data acquired from devices such as cameras and RFIDs to context aware services is being performed in the field on Life-Log and the sensor network. A variety of analytical techniques has been proposed to recognize various information from the raw data because video and audio data include a larger volume of information than other sensor data. However, manually watching a huge amount of media data again has been necessary to create supervised data for the update of a class or the addition of a new class because these techniques generally use supervised learning. Therefore, the problem was that applications were able to use only recognition function based on fixed supervised data in most cases. Then, we proposed a method of acquiring supervised data from a video sharing site where users give comments on any video scene because those sites are remarkably popular and, therefore, many comments are generated. In the first step of this method, words with a high utility value are extracted by filtering the comment about the video. Second, the set of feature data in the time series is calculated by applying functions, which extract various feature data, to media data. Finally, our learning system calculates the correlation coefficient by using the above-mentioned two kinds of data, and the correlation coefficient is stored in the DB of the system. Various other applications contain a recognition function that is used to generate collective intelligence based on Web comments, by applying this correlation coefficient to new media data. In addition, flexible recognition that adjusts to a new object becomes possible by regularly acquiring and learning both media data and comments from a video sharing site while reducing work by manual operation. As a result, recognition of not only the name of the seen object but also indirect information, e.g. the impression or the action toward the object, was enabled.

• 한국식품과학회지
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• 제10권2호
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• pp.194-202
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• 1978

In order to evaluate the utility of the anthocyanin pigments in Ganges Amaranth as an edible pigment, this study was designed to isolate and identify the anthocyanins. The anthocyanins present in leaves of Ganges Amaranth were extracted with 0.1% HCl in methanol. The extracted pigments were purified by organic solvent treatment and Amberlite CG-400 Type cation exchanger, and then separated into individual pigments by paper chromatography with n-butanol-formic acid-water(100:25:60, v/v) as a solvent system. The separated pigments were identified by their Rf values, sugar moieties, complete hydrolysis and spectral characteristics in the visible and ultraviolet regions. The amounts of individual anthocyanins were also determined. The results obtained from these experiments were as follows. 1. Chromatograms of the Ganges Amaranth extract developed with BFW yielded three anthocyanin bands. The two of the anothocyanin bands were tentatively identified as malvidin-3-glucoside(acylated with caffeic acid) in band 1 and peonidin-3-glucoside (acylated with caffeic acid) in band 2. But the anthocyanin in band 3 was not identified due to extremly low concentration. 2. The amount of total anthocyanins was 101.57 mg/100g fresh weight of leaves in which 82.15 mg of malvidin-3-glucoside (acylated with caffeic acid) and 27.20 mg of peonidin-3-glucoside(acylated with caffeic acid) were contained per 100g fresh weight. Maividin-3-glucoside acylated with the acid was, therefore, the most abundant pigment in the Ganges Amaranth.

• 대한물리의학회지
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• 제12권2호
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• pp.129-136
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• 2017

PURPOSE: The purpose of this study was to compare the gait of elderly patients with and without dementia to investigate the possibility of an ambulation assessment test as a diagnostic tool for dementia. METHODS: A total of 96 subjects were included with 60 participants without dementia (control group) and 36 patients with dementia (dementia group). To compare the walking ability of the two groups, a 4-m walking test (4MWT) and Groningen Meander Walking Test (GMWT) were conducted. The GMWT is graded by amount of time in seconds and by number of oversteps outside the track. Mann-Whitney U test was used to compare the gait between the groups and the area under the curve (AUC) with Received Operating Characteristic (ROC) curve was analyzed. Statistical significance was considered at a p<.05, with a 95% confidence interval. RESULTS: There were statistically significant differences (p<.05) between the dementia group and the control group for the 4MWT, GMWTSEC, and GMWTSTEP scores. The AUC was .95 for 4MWT, .92 for GMWTSEC, and .96 for GMWTSTEP with the 95% confidence interval. The cut-off values of the ROC curve were 1.03m/s for 4MWT, 10.8 second for GMWTSEC, and 3.75 steps for GMSTEP. CONCLUSION: In our study, we investigated the utility of ambulatory assessment tools to predict dementia. The results of this study suggest that the 4MWT and the GMWT used in this study are appropriate assessment tools for dementia prediction.