• Korean Journal of Microbiology
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• v.28 no.3
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• pp.188-191
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• 1990

The streptococcal plasmid pAM$\beta_{1}$ (erythromycin resistance)was transferred via conjugation from Streptococcus faecalis DS 5 to Lactobacillus casei YIT 9018 by a filter mating method. The transfer frequency depended greatly on the type, pore size and side(front or back) of membrane filter. Water passing through a membrane under reduced pressure induced very tight contact between the cells, increased the transconjugation frequency about 1.3 to 37-fold when Millipore membrane filter (0.45.$\mu$m, front side up) was used.

• Korean Journal of Construction Engineering and Management
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• v.23 no.1
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• pp.113-117
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• 2022

Additive manufacturing (AM, also known as 3D printing) technology is digitalized technology, making it easy to predict and manage quality and also, have design freedom ability. With these advantages, AM technology is applied to various industries. In particular, a method of manufacturing buildings and infrastructure with AM technology is being proposed to the construction industry. However, the application of AM technology is restricted due to problems such as insufficient history and quality of technology, lack of construction management methods, and certification of manufacturing products. Therefore, the manufacture of architectural products is implemented with indirect AM technology. In particular, it manufactures formwork using AM and injecting building materials to implement the architectural product. In this study, hybrid type material extrusion AM is used to manufacture large-sized formwork and implement building products. Moreover, we identify factors that can predict productivity and economic feasibility in the additive manufacturing process. As a result, design optimization results are proposed to reduce the production cost and time of architecture buildings.

• Bulletin of the Korean Chemical Society
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• v.13 no.2
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• pp.155-157
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• 1992

Extensive search over the energy surface of bicyclo[4.2.2]decapentaene with MMP2 molecular mechanics method and AM1 semiempirical MO method revealed only one, deep energy minimum structure, which corresponds to 1. The alternative structure 2 could not be identified as a stationary point. Although the deviation of benzenoid ring from planarity is large in the energy minimum structure (${\phi} = 26^{\circ}$(MMP2), $37^{circ}$ (AM1)), the bond lengths show no severe alternation.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 1999.10a
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• pp.131-136
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• 1999

This paper proposes the robot inverse calibration method using a neural networks. A highorder networks called Pi-Sigma networks has been used. The Pi-Sigma networks uses linear summing units in the hidden layer and product unit in output layer. The inverse calibration model which compensates the difference of joint variables only between measuring value and analytic value about the desired pose(position, orientation) of a robot is proposed. The compensated values are determined by using the weights obtained from the learning process of the neural networks previously. To prove the reasonableness, the SCARA type direct drive robot(4-DOF) and anthropomorphic robot(6-DOF) are simulated. It shows that the proposed calibration method can reduce the errors of the joint variables from $\pm$2$^{\circ}$to $\pm$ 0.1$^{\circ}$.

• YAKHAK HOEJI
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• v.37 no.4
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• pp.356-361
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• 1993

A sensitive method for the determination of methamphetamine(MA) and amphetamine(AM) in hair was developed by gas chromatography/mass spectrometry using stable isotope-labeled internal standards, amphetamine-d$_{5}$ and methamphetamine-d$_{5}$. Hair sample was washed with MeOH, incubated with MeOH(I% HCI) overnight at $37^{\circ}C$ while stirring and extracted using solid phase extraction column on a vacuum manifold. The extract obtained was pentafluoropropionated, and applied to GC/MS. The calibration curves of MA and AM were linear from 2.5 to 250 ng (r>0.99 for both). The limit of detection was 0.1 ng/mg in hair and cut-off level was set at 0.25 ng/mg for both. Hair samples of 27 MA abusers showed positive results in the range 0.7 to 106.8 ng/mg. AM, its metabolite, was detected in 20 out of 27 samples. The ratio of MA versus AM was 4.6~38.3 in specimens. Hair analysis for methamphetamine by GC/MS is an effective method for identifying long-term drug abusers.

• YAKHAK HOEJI
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• v.52 no.6
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• pp.419-425
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• 2008

A qualitative and quantitative analytical method was developed for detection of methamphetamine (MA) and its main metabolite amphetamine (AM) in oral fluid. Oral fluids of eleven drug abusers were provided by Police, specimens were collected by stimulation with a cotton swab treated with 20 mg of citric acid ($Salivette^{(R)}$; Sarstedt, USA). As the preliminary test, oral fluid samples were screened for amphetamines by Fluorescence Polarization Immunoassay (TDxFLx, Abbott Co.). Extraction for MA was performed using solid-phase extraction (SPE) by $RapidTrace^{TM}$ (Zymark, USA) with mixed mode cation exchange cartridge, CLEAN $SCREEN^{(R)}$ (130 mg/3 ml, UCT) after dilution with phosphate buffer. Samples were evaporated and derivatized by pentafluoropropionic acid anhydride (PFPA). Quantitation of MA and AM was performed by gas chromatography-mass spectrometry (GC-MS) using selective ion monitoring (SIM), the quantitation ions were m/z 204 (MA), 208 (MA-$D_5$), 190 (AM) and 194 (AM-$D_5$). The selectivity, linearity of calibration, limit of detection (LOD) and quantification (LOQ) within- and between day precision, accuracy and recoveries were examined as parts of the method validation. All oral fluid samples gave positive results to immunoassay for MA (cut-off level, 50 ng/ml as d-amphetamine). Concentrations of MA and AM by GC-MS in eleven samples were ranged 104.2${\sim}$4603.3 ng/ml and 32.4${\sim}$268.6 ng/ml, respectively. Extracted calibration curves of MA and AM were linear over the two concentration range of 1${\sim}$100 and 50${\sim}$1000 ng/ml with correlation coefficient of above 0.999. LOQ of MA and AM was 1 and 3 ng/ml, respectively. The intraand inter-day run precisions (CV) for MA and AM were less than 10%, and the accuracies (bias) for MA and AM were also less than 10% at the two different concentrations 5 and 100 ng/ml at low calibration range, 50 and 1000 ng/ml at high calibration range. The absolute recoveries of MA and AM at low and high calibration ranges were more than 82% and 75%, respectively. In this study the qualitative and quantitative analytical method of MA in oral fluid was established. Oral fluid testing may detect drug use in past hours because of its shorter detection window than urine, and be useful in post-accident situations. So oral fluids will be most useful for testing drug abuse in the driving under the influence of drug (DUID) as the alternative specimens of urine.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.10A
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• pp.1072-1077
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• 2007

In this paper, a power amplifier intermodulation distortion has been analyzed to improve linearity and the analysis results are used to minimize the distortion for linear power amplifier design. The proposed design technique is which the intermodulation distortions of the final amplifier are removed by driver intermodulation distortions. This proposed technique is based on AM to AM distortion analysis using power series, and AM to PM distortion analysis results using Bessel function. To verify this technique implement a cellular HPA(High Power Amplifier) 30W. From the results of the implementation and measurement for the linear power amplifier, the spurious characteristic is shown as 50 dBc at 1.98 MHz with 30 W with 20FA. These results show that distortion characteristics are improved as much as 10 dB in spurious characteristic compared with conventional design method.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.3
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• pp.514-522
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• 2015

In Microwave Radio Link, it is necessary to consider the radio fading to consistently assure the radio link of the high quality as the distance. If the fixed modulation is used for the microwave radio link, it is difficult to keep the high availability of link for the case of the long distance. Adaptive Modulation(AM) provide good quality on microwave link, but the application of the only-AM is not enough to provide the availability assurance due to the fluctuating characteristics of link capacity. Our previous study has proposed the improvement method of transmission in association with the distance, however the previous method needed to make up for the common application. There was no suggestion of a mathematical formula with a variable. In this paper, we propose an optimized Adaptive Transmission Techniques with calculating formula of the priority traffic transmission capacity based on AM in consideration of the fade margin for the availability assurance as the distance and the comparison of the channel bandwidth for Long-distance Radio Link.

• Journal of The Institute of Information and Telecommunication Facilities Engineering
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• v.2 no.4
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• pp.42-51
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• 2003

In this paper, we are going to analyze on relation of an output spectrum along phase distortion of power amplifier in wireless LAN system, and then considered an ACPR characteristic of power amplifier and consideration of an OFDM method for this. Also, we did implementation for OFDM modulation and transmission section of an IEEE 802.11a standard to have transmission speed of the maximum 54Mbps in order to know an OFDM modulation method and relation of non-linear characteristic of power amplifier. The non-linear characteristic of power amplifier did modeling with AM-to-AM and AM-to-PM, and we analyzed an output spectrum characteristic along phase distortion composed input signal supply for power amplifier. When output spectrum analysis results phase distortion increased, and an AM-to-PM characteristic of power amplifier in 5 degrees, the output spectrum was satisfied with a demand spectrum in P1 dB, but 10-20 degrees were able to confirm what cannot be satisfied with a demand spectrum in phase distortion. Also, an output spectrum of power amplifier by frequency re-growth generated by a non-linear characteristic of power amplifier did not satisfied in P1dE. therefore, a back-off value was requested according to an AM-to-PM distortion degree, and smaller back-off value were able to know what demand became in case of modulation section that used OFDM.

• Journal of Technologic Dentistry
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• v.37 no.4
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• pp.229-234
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• 2015

Purpose: The purpose of this study was to analysis the internal fitness of single crown fabricated by additive method technique. Methods: Study models were fabricated. Ten crowns were manufactured by additive method technique (AM group) and another ten crowns using conventional method were manufactured (CM group). The internal fitness of specimens were measured using silicone replica technique. Silicone replicas were sectioned one times (direction: from mesial to distal). The internal fitness were evaluated using by digital microscope. Statistical analyses were performed with independent samples t-test. (${\alpha}=0.05$). Results: Mean(SD) of all internal fitness were $92.1(20.0){\mu}m$ for AM group and $69.7(12.3){\mu}m$ for CM group. Two groups were statistically significant differences (p<0.001). Conclusion: All internal gaps of AM group were ranged within the clinical recommendation ($120{\ss}{\neq}$).