• Title/Summary/Keyword: AM1

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The variation of serological titers on the chickens infected pullorum disease from Kyongbuk provinces (경북지방유래 추백리 양성계에서의 균분리 및 혈청역가 추이)

  • 김영환;김경희;우용구;장영술;조민희;김수웅
    • Korean Journal of Veterinary Service
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    • v.20 no.1
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    • pp.19-26
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    • 1997
  • The present study was conducted to investigate the general epidemiological situations with 18-pullorum infected chickens from Kyongbuk provinces during the period from June 1995 to January 1996. On the Salmonella pullorum isolation tests by rectal swab culture method from infected chickens (386-samples), any Salmonella spp was not isolated from infected live-birds. But 2-S pullorum were isolated of 2-dead chickens(33.3% ) from 6-dead chickens which were positively reacted by serological tests. On the other hand, we could not isolated any Salmonella spp. in any parts of egg-contents ; egg-shell, egg-white and egg-yolks with 25-infected bird eggs. On the tests of antibiogram, 2-S pullorum strains were highly sensitive to GM, AM, SXT, CZ, K, FIM, ENR, C, AN, N, NN, LIN+SP, CF, TE and PB, respectively and intermediate sensitive to the CB, CFP, CL, S, P and XNL. But 2-strains were resistant to CC, DP, E, L, OX, TLA and TyLO. In the serological tests, pullorum antibody titers of 18-infected birds was from 2.76 to 9.18 with average by the microplate test. During the 6-months, pullorum antibody average titers were not changed generally. To validate the effects of the antimicrobial agent treatments to the serological antibody titers, infected 6-chickens was medicated with 0.5%-futazolidone. The titer of premeditated birds was average 4.26 but after medication with furazolidone, the titers of treated 6-birds was average 4.08.

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Screening of Novel Inducible Resistance Gene to Macrolide-Lincosamide-Streptogramin B (MLS) Antibiotics from Clinical Isolates of Staphylococcus spp (임상분리 Staphylococcus속 균주로부터 마크로라이드-린코사마이드-스트렙토그라민 B(MLS)계 항생물질에 대한 새로운 유도내성 유전자의 검색)

  • 오정자;권애란;이미정;김숙경;최성숙;최응칠;김병각
    • Biomolecules & Therapeutics
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    • v.1 no.2
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    • pp.177-182
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    • 1993
  • From 84 clinical isolates of Staphylococcus species, ten strains showing inducible resistance to MLS antibiotics were selected by disk agar diffusion method. Colony hybridization was executed using two MLS inducible resistance genes, ermA and ermC, previously identified from S. aureus as probes. S. hemolyticus 401 and S. epidermidis 542 whose genes were not homologous to those probes were finally selected. It was determined that the resistance genes of S. hemolyticus 401 and S. epidermidis 542 were not homologous to ermA, ermC and ermAM by Southern hybridization. S. epidermidis 542 had a plasmid DNA. To know if the plasmid may have genes related to inducible resistance, it was attempted to transform B. subtilis BR151 and S. aureus RN4220 with the plasmid prepared from S. epidermidis 542. It was shown that the gene related to inducible resistance to MLS antibiotics did not exist in this plasmid. These results indicate that two clinical isolates of S. hemolyticus 401 and S. epidermidis 542 had novel genes which were not homologous to MLS resistance genes identified previously. It was assumed that these genes may exist in chromosomal DNA.

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Comprehensive Identification of Tumor-associated Antigens via Isolation of Human Monoclonal Antibodies that may be Therapeutic

  • Kurosawa, Yoshikazu
    • IMMUNE NETWORK
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    • v.9 no.1
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    • pp.4-7
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    • 2009
  • Although the success of trastuzumab and rituximab for treatment of breast cancer and non-Hodgkins lymphoma, respectively, suggests that monoclonal antibodies(mAbs) will become important therapeutic agents against a wider range of cancers, useful therapeutic Abs are not yet available for the majority of the human cancers because of our lack of knowledge of which antigens (Ags) are likely to become useful targets. We established a procedure for comprehensive identification of such Ags through the extensive isolation of human mAbs that may be therapeutic. Using the phage-display Ab library we isolated a large number of human mAbs that bind to the surface of tumor cells. They were individually screened by immunostaining, and clones that preferentially and strongly stained the malignant cells were chosen. The Ags recognized by those clones were isolated by immunoprecipitation and identified by mass spectrometry(MS). We isolated 2,114 mAbs with unique sequences and identified 25 distinct Ags highly expressed on several carcinomas. Of those 2,114 mAbs 434 bound to specifically to one of the 25 Ags. I am going to discuss how we could select proper target Ags for therapeutic Abs and candidate clones are therapeutic agents.

Geometries and Relative Stabilities of AlN Four-Membered-Ring Compound Isomers: Ab initio Study

  • Park, Sung-Soo;Lee, Kee-Hag;Suh, Young-Sun;Lee, Chang-Hoon;Luthi, Hans P.
    • Bulletin of the Korean Chemical Society
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    • v.23 no.2
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    • pp.241-244
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    • 2002
  • Using ab initio method, we have studied the structural stabilities, the electronic structures and properties between the two isomers with $C_{2h}$ and $C_{2v}$ symmetry of AlN four-membered-ring single precursors $[Me_2AlNHR]_2$ (R = Me, $^iPr$, and $^iBu$). In the viewpoint of bond lengths in optimized structures, the N-C bonds are considerably affected by the change of the R groups bonded to nitrogen, but the bonding characters of the Al-N and Al-C bonds are little affected. Also the structural stabilities between the two isomers with $C_{2h}$ and $C_{2v}$ symmetry by using Hartree-Fock (HF) and the second order Moeller-Pleset (MP2) calculations agree well with the experimental results for the relative stability of bis(dimethyl- m-isopropylamido-aluminum) (BDPA) and bis(dimethyl- m-t-butylamido-aluminum) (BDBA), while the semiempirical AM1 and PM3 calculations for BDPA were reverse. Thus, our results may aid in designing an optimum precursor for a given process by explaining the experimental results through the elimination of the R groups bonded to nitrogen.

A Study of Hair Damage by Magic Straight Perm

  • Lim, Sun-Nye
    • Applied Microscopy
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    • v.42 no.3
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    • pp.129-135
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    • 2012
  • In this study, the changes in hair quality before and after Magic straight perm have been evaluated through a hair damage measurement method. For this, a healthy high school student's (age18 years) wavy hair was selected and permed on the left and right sides. Then, the changes caused by physical methods which were applied during the fl at iron-based Magic straight perm were evaluated based on the hair damage measurement method before and after the Magic straight perm. According to the protein release test after the Magic straight perm, 1.26% in average and 0.14% was observed in Cool Magic straight perm sample. In a field emission scanning electron microscopy (FE-SEM) test, saw teeth-shaped partial desquamation of cuticle cells and impurities were observed in the warm-treated hair sample. In atomic force microscope (AFM), line-profile is a method to represent roughness data on hair. According to analysis on 3-dimensional (3D) images, the hair with Cool Magic straight perm was lower than the hair with Warm Magic perm in terms of the color change of 3D images. In addition, vertical changes were observed in the hair with Cool Magic perm. As a result, irregular surface roughness was observed. This study proposed a method to minimize hair damage by cooling down the heat with the cool hair straightener as soon as the Warm Magic was finished.

Epicuticular Waxes and Stomata of Adult Scale Leaves of the Chinese Juniper Juniperus chinensis

  • Kim, Ki-Woo
    • Applied Microscopy
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    • v.42 no.3
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    • pp.124-128
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    • 2012
  • Leaf surface structures were investigated in the Chinese juniper Juniperus chinensis by scanning electron microscopy. Adult scale leaves were collected from the tree, air-dried at room temperature, and sputter-coated with gold without further specimen preparation. Approximately fi ve stomata were locally distributed and arranged in clusters on the leaf surface. Stomata were ovoid and ca. 40 ${\mu}m$ long. The epicuticular wax structures of J. chinensis leaves were tubules and platelets. Numerous tubules were evident on the leaf regions where stomata were found. The tubules were cylindrical, straight, and ca. 1 ${\mu}m$ in length. They almost clothed the stomatal guard cells, and occluded the slit-shaped stomatal apertures. Moreover, the wax ridges were flat crystalloids that were connected to the surface by their narrow side. They did not have distinct edges, and their width/height ratio varied. In particular, the wax ridges could be discerned on the leaf regions where stomata were not present nearby. Since the wax ridges did not have distinct edges on their margin, they were identified as platelets. Instances were noted where platelets were oriented either parallel to each other or perpendicular to the cuticle surface. These results can be used in biomimetics to design the hierarchical structures for mimicking the plant innate properties such as hydrophobicity and self-cleaning effects of the leaf surface.

Tips for English Presentations by Busy Doctors (바쁜 의사를 위한 영어발표 요령)

  • Song, Ho-Young
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.11 no.sup2
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    • pp.1-10
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    • 2008
  • When you present a medical scientific paper or give a lecture in English, It is important to visit your presentation room in advance to gain familiarity with the controls and the computer. You can check how to use the mouse or laser pointer and determine where the microphones are. You should meet the Chairman and introduce yourself. You may ask the Chairman any questions you have. I am confident this strategy will work for beginners because they are usually nervous before their presentations. Most Chairmen prepare one or two questions for the presenters to stimulate discussion. Speaking with the Chairman also allows you to determine the type of English that he or she speaks (i.e., French-English, Spanish-English, and British-English). You need to be comfortable with the Chairman's English before the presentation because the Chairman will repeat the questions for you when you do not understand a question from the audience. Although I have given a number of lectures and moderated many scientific sessions and symposiums in English over the past 20 years, it is still difficult and stressful for me to do these in English because English is not my first language. To overcome the problems, I have written down the questions and useful English expressions that I have received during or after my presentation because I found them helpful for my next presentation. I hope the examples will be of great help to you.

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A Study on the Epidemiological Characteristics of Industrial Ocular Injuries (직업성 안상해(眼傷害)에 관한 기술역학적 연구)

  • Lee, Won-Chul;Lee, Kang-Sook;Meng, Kwang-Ho;Lee, Seung-Han;Chung, Sang-Moon
    • Journal of Preventive Medicine and Public Health
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    • v.21 no.1 s.23
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    • pp.113-120
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    • 1988
  • A total of 181 cases of industrial ocular injuries hospitalized and treated at the St. Mary's Hospital, Seoul during the 5-year period was studied on their epidemiological characteristics. Of these patients 96.7% were males, and 43.6% were in twenties, 49.7% of the cases were injured on left eyes. There were no statistically significant differences in frequencies of study subjects by day of the week and month of the year. Most common injury hour were between 10- <12 AM(13.6%) and between 2- <4 PM(20%). Of the total 101 cases, 37.6% were working less than 6 months, and 73.7%, less than 2 years. Of the total, 42.3% were involved in machinary work and 13.9%, in construction work. According to the American National Standard Method of recording basic facts relating to the nature and occurrence of work injuries, 62%, among all source of injuries was metal item and the most common accident type was struck by flying objects(76.2%). Among 181 cases, only 2 cases were wearing protective equipment when the accidents occurred.

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Surgical Treatment of an Aneurysm on a Branch of the Subclavian Artery, and the Mass Presented as a Neck Mass - A case report - (목 부위 종괴로 발현된 우측 쇄골하 동맥 분지에 발생한 동맥류의 수술적 치험 - 1예 보고 -)

  • Lee, Woo-Surng;Kim, Yo-Han;Chee, Hyun-Keun;Hwang, Jae-Joon;Kim, Jun-Seok;Lee, Song-Am
    • Journal of Chest Surgery
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    • v.42 no.3
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    • pp.380-383
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    • 2009
  • Subclavian artery aneurysms are a very rare disease and the common causes are trauma, infection and atherosclerosis. A 69-year-old female, who had no history of trauma and infection, underwent surgical treatment for an aneurysm on a branch of the subclavian artery, and this aneurysm presented as a neck mass.

Study on Visual Rhetoric (영상의 수사적 표현 연구)

  • 안상혁
    • Archives of design research
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    • v.16 no.4
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    • pp.207-214
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    • 2003
  • The reason why rhetoric is important to a moving image is that a moving image communicates through non-verbal communication language. At first, rhetoric in a moving image functions to be persuasive in the story of a moving image. Second, rhetoric works to enhance an aesthetic value for a moving image. Thus we feel good when we are watching a moving image with well embeded rhetoric. On the other hand, we see extened rhetoric to enhance an imaginary because of a hollywood movie with visual effect and a digital movie. Visual images now formed using more signigier compared to signified for signification. Also we see that every signifier structured metaphorically to function as a sign. In this paper, 1 am going to explain the principles of rhetoric applied to a moving image so that we can better understand a signification on visual image.

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