• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.412-416
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• 2011

최근 지구 온난화로 인한 자연환경 및 기상이변 등으로 초대형 태풍, 국지성 폭우, 집중호우 등 홍수로 인한 재해가 매년 증가하고, 피해규모 또한 증대되고 있는 실정이다. 기상청 60개 관측소를 기준으로 한 자료에 따르면 기후변화 등의 영향으로 최근 10년간('99 ~ '08) 1일 100mm 이상 집중호우 발생빈도가 연평균 97회로 '70 ~ '80년의 연평균 68회에 비해 1.4배 증가하였다. 이처럼 홍수발생의 빈도 및 강도가 증가하는 추세이며 이에 따라 홍수예보시스템의 중요도 또한 높아지게 되었다. 따라서 본 연구에서는 기존 국내 운영중인 홍수예경보시스템 뿐만 아니라 국외 홍수예보시스템 사례조사를 통해 현재 운영중인 시스템 및 홍수예경보 관련모형들의 웹 기반 모듈화 현황 및 적용가능성을 검토하고, 한국형 선진수문예측시스템으로 구축하기 위한 기본 방향을 제안하고자 한다.

• The Journal of Korean Physical Therapy
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• v.15 no.3
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• pp.1-15
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• 2003

The purpose of this study was to evaluate the change of lower extremity alignment according to plantar surface compliance in standing status. The thirty normal adults (15 men and 15 women) aged between 20 and 29 were assigned to 3 groups: control group, experimental groups (AHPS group, ASPH group). The lower extremity alignment was examined before and after adaptation with corresponding foam types. The results of this study were as follows : 1. As the result of comparing lower extremity alignment before and after test of the control group which any change did not exist in plantar surface compliance, there were not significant differences in the angle A, B, C, D, E of right and left(p>0.05). 2. As the result of comparing lower extremity alignment before and after test of the AHPS group which forefoot part of plantar surface is hard and rear foot part is soft, there were not significant differences in the angle A of right and left(p>0.05). But, there were significantly decreased in the angle B, C, D, E of right and left(p<0.05). 3. As the result of comparing lower extremity alignment before and after test of the ASPH group which forefoot part of plantar surface is soft and rear foot part is hard, there were not significant differences in the angle A of right and left(p>0.05). But, there were significantly increased in the angle B, C, D, E of right and left(p<0.05). 4. As the result of comparing lower extremity alignment of the control group, AHPS group and ASPH group, there were not significant differences in the angle A, B, C, D, E of right and left before test(p>0.05). But, there were significant differences in the angle C, D of right and left after test(p<0.05).

• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1620-1627
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• 2009

The design and expression of an antihypertensive peptide multimer (AHPM), a common precursor of 11 kinds of antihypertensive peptides (AHPs) tandemly linked up according to the restriction sites of gastrointestinal proteases, were explored. The DNA fragment encoding the AHPM was chemically synthesized and cloned into expression vector pGEX-3X. After an optimum induction with IPTG, the recombinant AHPM fused with glutathione S-transferase (GST-AHPM) was expressed mostly as inclusion body in Escherichia coli BL21 and reached the maximal production, 35% of total intracellular protein. The inclusion body was washed, dissolved, and purified by cation-exchange chromatography under denaturing conditions, followed by refolding together with size-exclusion chromatography and gradual dialysis. The resulting yield of the soluble GSTAHPM (34 kDa) with a purity of 95% reached 399 mg/l culture. The release of high active fragments from the AHPM was confirmed by the simulated gastrointestinal digestion. The results suggest that the design strategy and production method of the AHPM will be useful to obtain a large quantity of recombinant AHPs at a low cost.

• Molecules and Cells
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• v.25 no.2
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• pp.294-300
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• 2008

Cytokinins are essential hormones in plant development. $\underline{A}$rabidopsis $\underline{h}$istidine-containing $\underline{p}$hosphotransfer proteins (AHPs) are mediators in a multistep phosphorelay pathway for cytokinin signaling. The exact role of AHP4 has not been elucidated. In this study, we demonstrated young flower-specific expression of AHP4, and compared AHP4-overexpressing (Ox) trangenic Arabidopsis lines and an ahp4 knock-out line. AHP4-Ox plants had reduced fertility due to a lack of secondary cell wall thickening in the anther endothecium and inhibition of IRREGURAR XYLEMs (IRXs) expression in young flowers. Conversely, ahp4 anthers had more lignified anther walls than the wild type, and increased IRXs expression. Our study indicates that AHP4 negatively regulates thickening of the secondary cell wall of the anther endothecium, and provides new insight into the role of cytokinins in formation of secondary cell walls via the action of AHP4.

• Toxicological Research
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• v.13 no.4
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• pp.353-357
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• 1997

The possibility of the allergic encephalomyelitis caused by the combined vaccine (KGCC95VI) for the prophylaxis against Japanese encephalitis and Hantaan virus infection, recently developed by Korea Green Cross Corporation, was investigated in the Hartley guinea pigs. The KGCC-95VI was administered to the guinea pigs subcutaneously to sensitize the animals three times at one month intervals. There were no clinical signs or gross pathological findings. There were no abnormal histopathological findings at cerebrums, cerebellums, brain stems and the spinal cords. The concentration of myelin basic protein was 1.10 ng/dose quantified by ELISA, which met the guide4ine of below 2 ng/ml/dose recommended by American Society of Health -System Pharmacists(AHPS) Drug Information. Accordingly, the KGCC-95VI is considered not to induce any allergic immune responses which may lead to the experimental allergic encephalomyelitis.