• Restorative Dentistry and Endodontics
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• 제39권4호
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• pp.282-287
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• 2014

Objectives: The aim of the present study was to investigate the bond strength of RelyX Unicem (3M) to root canal dentin when used as an endodontic sealer. Materials and Methods: Samples of 24 single-rooted teeth were prepared with Gates Glidden drills and K3 files. After that, the roots were randomly assigned to three experimental groups (n = 8) according to the filling material, (1) AH Plus (Dentsply De Trey GmbH)/Gutta-Percha cone; (2) Epiphany SE (Pentron)/Resilon cone; (3) RelyX Unicem/Gutta-Percha cone. All roots were filled using a single cone technique associated to vertical condensation. After the filling procedures, each tooth was prepared for a push-out bond strenght test by cutting 1 mm-thick root slices. Loading was performed on a universal testing machine at a speed of 0.5 mm/min. One-way analysis of variance and Tukey test for multiple comparisons were used to compare the results among the experimental groups. Results: Epiphany SE/Resilon showed significantly lower push-out bond strength than both AH Plus/Gutta-Percha and RelyX Unicem/Gutta-Percha (p < 0.05). There was no significant difference in bond strength between AH Plus/Gutta-Percha and RelyX Unicem/Gutta-Percha (p > 0.05). Conclusions: Under the present in vitro conditions, bond strength to root dentin promoted by RelyX Unicem was similar to AH Plus. Epiphany SE/Resilon resulted in lower bond strength values when compared to both materials.

• Restorative Dentistry and Endodontics
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• 제28권2호
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• pp.162-168
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• 2003

이 연구는 기존의 레진 근관봉함재를 보완하여 개발한 근관봉함재(Adseal; 새로운 레진 계통의 근관봉함재)를 이미 상품화된 레진 계통의 근관봉함재(AH 26, AH Plus), 산화 아연 유지놀 계통의 근관봉함재(TubliSeal EWT, Pulpcanal sealer EWT), 수산화 칼슘 계통의 근관봉함재(Sealapex)와 비교하여 세포독성과 항균작용을 평가하고자 한다. 세포독성 실험은 L929 쥐의 섬유아세포를 사용하여 세포의 viable ratio를 계산한 후, Giemsa stain으로 염색하여 세포의 양상을 관찰하였고, 항균작용 실험은 Enterococcus faecalis Porphyromonas endodontalis, Porphyomonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum 와 Fusobacterium necrophorum를 사용하여 agar diffusion test로 평가한다. Adseal은 다른 근관봉함재에 비해 훨씬 낮은 세포독성을 보였고, AH Plus, AH 26, TubliSeal EWT, Sealapex, Pulpcanal sealer EWT의 순으로 세포독성의 정도가 높아짐을 알 수 있었다. 또한 Adseal은 Enterococcus faecalis 에서는 낮은 항균작용을 보이지만, Black-pigmented bacteria 에서는 높은 항균작용을 보이는데, 모든 근관봉함재는 서로 다른 종에 따라 어느 정도의 항균효과를 가지고 있음을 알 수 있었다.

• Restorative Dentistry and Endodontics
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• 제45권2호
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• pp.18.1-18.9
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• 2020

Objectives: The purpose of this study was to evaluate the void of root canal filling over time when a calcium silicate sealer was used in the single gutta-percha cone technique. Materials and Methods: Twenty-four J-shaped simulated root canals and twenty-four palatal root canals from extracted human maxillary molars were instrumented with ProFile Ni-Ti rotary instruments up to size 35/0.06 or size 40/0.06, respectively. Half of the canals were filled with Endoseal MTA and the other half were with AH Plus Jet using the single gutta-percha cone technique. Immediately after and 4 weeks after the root canal filling, the samples were scanned using micro-computed tomography at a resolution of 12.8 ㎛. The scanned images were reconstructed using the NRecon software and the void percentages were calculated using the CTan software, and statistically analyzed by 1-way analysis of variance, paired t-test and Tukey post hoc test. Results: After 4 weeks, there were no significant changes in the void percentages at all levels in both material groups (p > 0.05), except at the apical level of the AH Plus Jet group (p < 0.05) in the simulated root canal showing more void percentage compared to other groups. Immediately after filling the extracted human root canals, the Endoseal MTA group showed significantly less void percentage compared to the AH Plus Jet group (p < 0.05). Conclusions: Under the limitations of this study, the Endoseal MTA does not seem to reduce the voids over time.

• 대한소아치과학회지
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• 제44권1호
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• pp.20-27
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• 2017

본 연구는 포졸란 시멘트를 기반으로 하는 근관전색제인 Endoseal MTA (Maruchi, Wonju, Korea)를 근관전색제와 근관충전제로 사용하였을 때 나타나는 치근단부 미세누출을 기존의 근관전색제인 AH $Plus^{(R)}$ (Dentsply DeTrey, Konstanz, Germany)와 비교하여 평가하고자 하였다. 만곡되지 않은 영구 전치를 3개의 실험군(A군, E1군, E2군)으로 나누고 A군은 AH $Plus^{(R)}$와 gutta-percha (GP), E1군 은 Endoseal MTA와 GP, E2군은 Endoseal MTA 단독으로 충전하였다. 충전이 완료된 치아는 완전한 경화를 위해 24시간동안 멸균 증류수에 보관하였고 염색액 누출을 위해 치근단 부위를 0.2% 로다민 B 염색 용액에 24시간 동안 노출시켰다. 입체 현미경을 이용하여 미세누출 정도를 평가하였으며 3개의 실험군 사이에 유의한 차이가 관찰되지 않았다. 결론적으로 Endoseal MTA는 GP의 사용에 관계 없이 AH $Plus^{(R)}$와 유사한 밀폐력을 보였으며 이러한 결과는 Endoseal MTA의 근관전색제 및 근관 충전제로써 유용한 사용에 대한 가능성을 제시한다.

• 대한치과재료학회지
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• 제46권1호
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• pp.1-10
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• 2019

근관치료 후 발생하는 치아변색은 여러 가지 원인에 의해 나타나는 것으로 알려져 있다. 본 실험은 근관치료 후 치아변색이 일어나는 여러 원인 중 실러 종류에 따른 영향을 평가하고 변색을 치료하기 위한 방법으로 실활치 미백을 시행할 경우 미백 효과 정도를 비교해 보는 것이다. 24개의 치아를 대조군, AH plus, Endosequece BC, MTA fillapex군 4개의 군으로 나누어 실험하였다. 실러를 사용하여 통상적인 방법으로 근관충전을 시행한 후 과붕산나트륨을 이용하여 실활치 미백을 시행 하였다. 그 후 Vita easyshade를 사용하여 치아의 색을 CIE L^*a^*b 시스템으로 측정하였다. 근관충전 후 변색실험에서 대조군과 모든 실험군에서 시간에 따라 치아의 색이 변하였다. 미백제의 효과에 대해서는 Endosequence BC와 MTA fillapex에서 AH plus군에 비해 미백효과가 유의하게 높은 결과가 나타냈다. 본 연구를 바탕으로 근관치료 시 실러의 주의 깊은 사용이 필요하며, epoxy resin 계열에 비해 Calcium silicate 계열의 실러로 인한 치아 변색에서 과붕산나트륨을 이용한 미백은 효율적일 수 있다.

• 대한치과보존학회:학술대회논문집
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• 대한치과보존학회 2001년도 추계학술대회(제116회) 및 13회 Workshop 제3회 한ㆍ일 치과보존학회 공동학술대회 초록집
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• pp.581.2-581
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• 2001

The purpose of this study was to compare the biocompatibility of AH 26, AH Plus, Pulp Canal Sealer, Adseal-1 and Adseal-2(newly developed resin-based sealers) using subcutaneous implantation test. 64 Sprague-Dawley rats were used. Three rats were used for each sealer for every experimental period (1, 2, 4, 12 weeks). The teflon tubes (5mm length, 1.5mm diameter) were washed with ethanol and distilled water and autoclaved. After anethesia four subcutaneous pockets (depth > 10mm) were prepared in each animal.(omitted)

• Restorative Dentistry and Endodontics
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• 제29권6호
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• pp.498-503
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• 2004

The properties of ideal root canal sealers include the ability of sealing the total root canal system and no toxic effects to periradicular tissues. Cytotoxicity test using cell culture is a common screening method for evaluation of the biocompatibility of root canal sealers. The purpose of this study was to investigate the cytotoxic effect of newly developed resin-based sealer (Adseal 1, 2, and 3) comparing with those commercial resin-based sealers (AH26 and AH Plus), ZOE-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT) and calcium hydroxide based sealer (Sealapex), An indirect contact test of cytotoxicity by agar diffusion was performed according to the international standard ISO 10993-5. L929 fibroblast cells were incubated at $37^{\circ}C$ in humidified 5% $CO_2-containing$ air atmosphere. The freshly mixed test materials were inserted into glass rings of internal diameter 5 mm and height 5 mm placed on the agar. After the 24 hrs incubation period, the decolorization zones around the test materials were assessed using an inverted microscope with a calibrated screen. A Decolorization Index was determined for each specimen. Adseal 1. 2, and 3 did not exert any cytotoxic effects, whereas AH26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, and Sealapex produced mild cytotoxicity.

• Restorative Dentistry and Endodontics
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• 제35권5호
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• pp.335-343
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• 2010

연구목적: 본 연구의 목적은 수종의 sealer를 이용하여 각기 다른 양의 잔여수분이 존재하는 근관을 충전 시, 근관충전의 sealing ability를 glucose leakage model로 평가하는 것이다. 연구 재료 및 방법: 발치된 90개의 단근치를 치관부를 잘라내고 #45 / 0.06 taper까지 확대한 후, 12개의 실험군 (n = 7)과 2개의 대조군 (n = 3)으로 나누었다. 실험군을 3가지 습윤 상태에서 (a, DRY; b, PAPER POINT DRY; c, WET) 4종류의 sealer로 (Group 1-3: Sealapex; Group 4-6: AH plus; Group 7-9: Tubuli-seal; Group 10-12: EndoRez), warm vertical compaction method를 이용해 충전하였다. $37^{\circ}C$, 습도 100%에서 7일 동안 보관하고 glucose leakage model을 이용하여 치관부로부터 치근부 방향의 미세누출을 정량화하였다. 1, 3, 7, 14, 21, 30일 째 누출된 glucose의 농도를 spectrophotometer로 측정하였다. 결과: 관찰기간 동안 AH plus/DRY군 (Group 4)이 12개 그룹 중 가장 적은 양의 leakage를 보였다 (20.78 mmol/L). 반면에 Tubuli-seal/WET군 (Group 9)이 12개 그룹 중 가장 높은 양의 leakage를 보였다 (178.75 mmol/L). 결론: 본 연구의 결과는 충전 시 근관 내의 습윤 상태와 sealer 종류가 microleakage에 유의한 영향을 미친다는 것을 입증하였다. 그러므로 sealer 종류에 따라 각기 다른 근관 건조 방법을 사용하는 것이 근관치료에서 더 좋은 결과를 얻을 수 있는 방법일 것이다.

• Preventive Nutrition and Food Science
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• 제20권4호
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• pp.230-237
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• 2015

This study investigated the impact of microalgal oil (MO) on body weight management in C57BL/6J mice. Obesity was induced for 8 weeks and animals were orally supplemented with the following for 8 additional weeks: beef tallow (BT), corn oil, fish oil (FO), microalgal oil (MO), or none, as a high fat diet control group (HD). A normal control group was fed with a normal diet. After completing the experiment, the FO and MO groups showed significant decreases in body weight gain, epididymal fat pad weights, serum triglycerides, and total cholesterol levels compared to the HD and BT groups. A lower mRNA expression level of lipid anabolic gene and higher levels of lipid catabolic genes were observed in both FO and MO groups. Serum insulin and leptin concentrations were lower in the MO group. These results indicated that microalgal oil has an anti-obesity effect that can combat high fat diet-induced obesity in mice.

• Restorative Dentistry and Endodontics
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• 제38권4호
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• pp.204-209
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• 2013

Objectives: The objective of this in vitro study was to evaluate and compare the cytotoxicity of four different root canal sealers i.e. Apexit Plus (Ivoclar Vivadent), Endomethasone N (Septodont), AH-26 (Dentsply) and Pulpdent Root Canal Sealer (Pulpdent), on a mouse fibroblast cell line (L929). Materials and Methods: Thirty two discs for each sealer (5 mm in diameter and 2 mm in height) were fabricated in Teflon mould. The sealer extraction was made in cell culture medium (Dulbecco's Modified Eagle's Medium, DMEM) using the ratio 1.25 $cm^2/mL$ between the surface of the sealer samples and the volume of medium in a shaker incubator. Extraction of each sealer was obtained at 24 hr, 7th day, 14th day, and one month of interval. These extracts were incubated with L929 cell line and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was done. Two-way ANOVA for interaction effects between sealer and time and Post-hoc multiple comparison using Tukey's test across all the 16 different groups were used for statistical analysis. Results: Apexit Plus root canal sealer was significantly less toxic than other sealers (p < 0.05) and showed higher cellular growth than control. Endomethasone N showed mild cytotoxicity. AH-26 showed severe toxicity which became mild after one month while Pulpdent Root Canal Sealer showed severe to moderate toxicity. Conclusions: Apexit Plus was relatively biocompatible sealer as compared to other three sealers which were cytotoxic at their initial stages, however, they became biocompatible with time.