• Title/Summary/Keyword: AH 26

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Molecular Characterization of Some Antilisterial Bacteriocin Genes from Enterococcus faecium and Pediococcus pentosaceus

  • El-Arabi, Nagwa I.;Salim, Rasha G.;Abosereh, Nivien A.;Abdelhadi, Abdelhadi A.
    • Microbiology and Biotechnology Letters
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    • v.46 no.3
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    • pp.288-299
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    • 2018
  • Food bio preservation is of major interest in the food industry. Many types of antimicrobial compounds can be produced by lactic acid bacteria (LAB), including bacteriocins. Bacteriocins increase the shelf-life of food by decreasing some food-borne diseases. In this study, a multi-coding sequence of bacteriocin genes was used for primer design to produce bacteriocin genes in Enterococcus faecium AH2 strain and Pediococcus pentosaceus AH1. Multi-coding sequences were aligned to detect conserved sequences in the bacteriocin gene. Eight genes encoding proteins involved in bacteriocin production were isolated and sequenced, including six from E. faecium AH2 (entA, entI, entF, entR, orfA2, orfA3) and two from P. pentoceseus AH1 (papA, pedB), and all gene sequences were deposited in the Gen Bank database under accession numbers LC064146-LC064151, LC101300, and LC101789, respectively. P. pentosaceus AH1 and E. faecium AH2 strains displayed bacteriocin activities of $2610AU\;mL^{-1}$ and $690AU\;mL^{-1}$ and inhibition zones of 26 mm and 19 mm, respectively. Overexpression of entA in E. faecium AH2 increased the bacteriocin and antimicrobial activities.

The Comparison of the Thermal and Mechanical Characteristic in Butt Joint for Ship Structure Thick Plate AH32 Steel by SAW & Hybrid(CO2 Laser+MIG)Welding (조선용 후판 AH32 강에 대한 SAW 및 Hybrid(CO2 Laser+MIG) 맞대기 용접부의 열 및 역학적 특성 비교)

  • Bang, Han-Sur;Oh, Chong-In;Bang, Hee-Seon;Ro, Chan-Seung;Lee, Yoon-Ki;Bong, Hyun-Soo;Lee, Jeong-Soo
    • Journal of Welding and Joining
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    • v.26 no.5
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    • pp.29-35
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    • 2008
  • In this paper concentrate on the comparison of the thermal and mechanical characteristics in Butt joint of ship structure AH32 steel by using hybrid welding and conventional SAW. For this purpose, fundamental welding phenomena of hybrid process using $CO_2$ Laser and MIG is investigated by the experiments and characteristics of thermal and welding residual stress distribution of welded joint in SAW and hybrid welding are understood from the result of FE numerical simulation and experimental values. From the result of this study, it is understood that Laser-MIG hybrid welding have high potential, make substantial saving of time and manufacturing cost and may proves its self robust in the butt joining of thick AH32 steel ship structural plate in the near future.

Biocompatibility of two newly-developed resin-based root canal sealers

  • Kim, Yong-Beom;Bae, Kwang-Shik
    • Proceedings of the KACD Conference
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    • 2001.11a
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    • pp.581.2-581
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    • 2001
  • The purpose of this study was to compare the biocompatibility of AH 26, AH Plus, Pulp Canal Sealer, Adseal-1 and Adseal-2(newly developed resin-based sealers) using subcutaneous implantation test. 64 Sprague-Dawley rats were used. Three rats were used for each sealer for every experimental period (1, 2, 4, 12 weeks). The teflon tubes (5mm length, 1.5mm diameter) were washed with ethanol and distilled water and autoclaved. After anethesia four subcutaneous pockets (depth > 10mm) were prepared in each animal.(omitted)

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Secure VPN Performance in IP Layers (IP계층에서의 VPN 전송성능에 관한 연구)

  • 임형진;권윤주;정태명
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.26 no.11C
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    • pp.102-112
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    • 2001
  • This paper analyzes Security Performance and Processing Performance to measure performance between nodes by using AH and ESP protocol. IPsec VPN provides application with security service implemented in IP Layer while traffic cost and packet processing time it increased by encryption, decryption and authentication in AH and ESP. We measured overall packet processing time and IPsec module processing time. The result of the efficiency test showed that the factors of influencing electrical transmission efficiency were the size of electrical transmission packets, codes used for tunnelling, authentication functions, CPU velocity of host7, and the embodiment of IPsec; for a high capacity traffic, IPsec transmission was not appropriate, because transmission velocity was delayed by more than ten times in comparison with Non-IPsec.

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Manufacturing of Burst mode Transceiver module and Performance Test for Upstream Channel of Gigabit Ethernet PON System (GE-PON 시스템을 위한 버스트 모드 광수신기 제작과 상향채널 특성 평가)

  • Chang, Jin-Hyeon;Jung, Jin-Ho
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.12 no.2
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    • pp.167-174
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    • 2012
  • The circuits including with Optical transceiver and clock data recovery, in this paper, SERDES (SERializer-DESerializer) are implemented to construct a GE-PON burst-mode transceiver supporting IEEE 802.3ah and a jig for measuring the burst-mode characteristics, that is to say, PON upstream optical transmission environment are manufactured to evaluate the performance of transceiver. we verified that the limiting amplifier compensated the gap of max. 26dB optical power by experiments. The startup acquisition lock time is 670ns in case of using VSC7123 and 2300ns in case of S2060 and the data acquisition lock time were measured to be 400ns and 600ns, respectively, in the upstream channel transmission in this work. While on the other, VSC7123 is satisfied with IEEE802.3ah recommendations.

Anti-lost Device Design using Bluetooth4.1 (블루투스4.1 기반 소형 분실방지용 송수신회로 설계)

  • Chae, Gyoo-Soo
    • Journal of Convergence Society for SMB
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    • v.6 no.4
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    • pp.25-30
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    • 2016
  • This paper presents on the development of a compact anti-lost device requested recently. The proposed device consists of the master and slave modules based on Bluetooth4.1 technology. To implement a low-power characteristic, an algorithm has been also developed. The transmitting and receiving circuits are designed by using BoT CLE110 module supporting Bluetooth 4.1. The ATmega 328P-AU was used for the control and LP3874EMP was used as a linear regulator. Power consumption of the fabricated product in operating mode is only 10mAh and 35mAh for MCU operating state. Alarm operation distance is $10m{\pm}30%$, the effective radiated power is less than 10mW, the frequency band is designed to operate in the Bluetooth band with 26MHz bandwidth. And algorithms have been developed to extend the battery life. The size of the product was obtained as $45{\times}45{\times}15mm$ for master and $35{\times}35{\times}10mm$ fr slave. After the optimization process, it is expected to be commercialized as a wristwatch for anti-lost device.

CYTOTOXICITY OF RESIN-BASED ROOT CANAL SEALER, ADSEAL (레진 계열 근관봉함재 Adseal의 세포독성에 관한 연구)

  • Kim, Hee-Jung;Baek, Seung-Ho;Lee, Woo-Cheol;Park, Han-Soo;Bae, Kwang-Shik
    • Restorative Dentistry and Endodontics
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    • v.29 no.6
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    • pp.498-503
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    • 2004
  • The properties of ideal root canal sealers include the ability of sealing the total root canal system and no toxic effects to periradicular tissues. Cytotoxicity test using cell culture is a common screening method for evaluation of the biocompatibility of root canal sealers. The purpose of this study was to investigate the cytotoxic effect of newly developed resin-based sealer (Adseal 1, 2, and 3) comparing with those commercial resin-based sealers (AH26 and AH Plus), ZOE-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT) and calcium hydroxide based sealer (Sealapex), An indirect contact test of cytotoxicity by agar diffusion was performed according to the international standard ISO 10993-5. L929 fibroblast cells were incubated at $37^{\circ}C$ in humidified 5% $CO_2-containing$ air atmosphere. The freshly mixed test materials were inserted into glass rings of internal diameter 5 mm and height 5 mm placed on the agar. After the 24 hrs incubation period, the decolorization zones around the test materials were assessed using an inverted microscope with a calibrated screen. A Decolorization Index was determined for each specimen. Adseal 1. 2, and 3 did not exert any cytotoxic effects, whereas AH26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, and Sealapex produced mild cytotoxicity.

Synthesis of Neplanocin A Analog with 2′-“up”-C-Methyl Substituent as Potential Anti-HCV Agent

  • Lee, Hyung-Rock;Kang, Jin-Ah;Park, Ah-Young;Kim, Won-Hee;Chun, Pu-Soon;Kim, Jung-Su;Kim, Jin-Ah;Lee, Bo-Eun;Jeong, Lak-Shin;Moon, Hyung-Ryong
    • Bulletin of the Korean Chemical Society
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    • v.30 no.9
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    • pp.2043-2050
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    • 2009
  • 2′-$\beta$-C-Methylneplanocin A (3) was synthesized via 2-$\beta$-C-methylribonolactone, prepared by a modified Whistler and BeMiller’s method developed by our laboratory, as potential anti-HCV agent. Reduction of 14 with Dibal-H afforded 26 in a good yield with a trace of 25, whereas a Luche reduction gave 26/25 = 4/1 mixture. Several attempts were made to chemoselectively remove TBS group in the presence of TBDPS group and treatment with both PPTS and TsOH showed the best result. Condensation of 26 with 6-chloropurine under Mitsunobu conditions produced an $S_N$2 product 27 along with an $S_N$2′ product 28.

HISTOLOGIC STUDY ON THE PERIODONTAL TISSUE REACTIONS OF THE ROOT PERFORATIONS (치근관(齒根管) 천공(穿孔)에 의(依)한 치주조직(齒周組織) 변화(變化)에 관(關)한 조직학적(組織學的) 연구(硏究))

  • Park, Sang-Jin
    • Restorative Dentistry and Endodontics
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    • v.6 no.1
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    • pp.7-16
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    • 1980
  • The author observed the periodontal tissue reactions to the root canal sealers after root perforations were made intentionally in dogs. The perforations were made on 74 teeth from 7 dogs. The experiments were performed in two different modes of procedure: In Group I, the perforations were made through the root canal to the alveolar bone. In Group II, the perforations were made from site of alveolar bone to the root canals. The perforated canals in Group I were filled with gutta-percha and root canal cements; Calxyl (Calcium Hydroxide in Ringer's solution), Zinc Oxide -Eugenol cement (Z.O.E.), Kerr sealer (Rickert's paste) and AH 26 (Epoxy Resin preparations). The perforated canals in Group II were sealed with Calxyl, Z.O.E, Kerr sealer and AH26. Histologic examinations of periodontal tissue reactions were observed at various time intervals. The results were as follows; l. Cementum deposition on the perforated root surface in Group II cases showed slightly earlier than that of Group I. Healing tendency of injured alveolar bone in Group II was greater than that of Group I. 2. According to the time increase after experiment, the cementum deposition on the site of perforated dentin in Group II with intact pulp was notably thickened. Secondary dentin deposition on the root canal surface where the dentinal tubles were cut was also found in similar pattern. 3. In the cases of perforated canals sealed with Calxyl both in Group I and Group II, It revealed the earliest cementum-deposition among 4 different root canal cements. In the cases of perforated canals sealed with Kerr sealer and AH26, the cementum-deposition on the root surface was not found. 4. Proliferation of epithelium around the perforated area was first seen at 5-week cases in Group I, and at 3-week cases in Group II. 5. In all cases, dentin resorption on the site of perforated root surface was always occured.

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