• The Plant Pathology Journal
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• v.15 no.1
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• pp.68-71
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• 1999

To illustrate the action mechanism of pencycuron on Rhizoctonia solani, two experiments were conducted including the comparison of amino acids of $\beta$-tubulin between R-C (sensitive isolate) and Rh-131 (non-sensitive isolate), and the inhibitory effect of pencycuron on tubulin assembly in vitro. Both $\beta$-tubulin genes of R-C and Rh-131 proved to have 1,582 nucleotides encoding a protein of 445 amino acids, showing 98% homology in amino acid sequences between them. It was found that codons at 103, 236, and 267 for lysine (AGG), valine (GTC) and isoleucine (ATT) in R-C were replaced by codons for methionine (ATG), isoleucine (ATT) and methionine (ATG) in Rh-131, respectively. No inhibitory effect of pencycuron on the tubulin assembly was observed. It suggests that pencycuron may have no direct inhibitory effects on the assembly of tubulin at least in vitro.

• Journal of Life Science
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• v.6 no.1
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• pp.34-39
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• 1996

The biopolymer membrane could be formed using marine polysaccharides. Chiotsan and alginate were used for the formation of capsule membrane to mimic the fish eggs such as flying fish eggs and salmon eggs. The size of capsules ranging 1 to 5mm was prepared and the mechanical tests were performed to determine the mechanical similarities to natural fish eggs. The similar mechanical pattern between the synthetic capsules and natural eggs could be found. The controlling parameters for the strength of capsules were pH of the chitosan solution. This encapsulation technique can be broadly applied to medical, engineering as well as food areas.

• YAKHAK HOEJI
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• v.37 no.5
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• pp.504-510
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• 1993

Human insulin gene is consisted of the polymorphic region with the repeating units, the regulatory sequence, the structural gene including the intervening sequence, and 3'-flanking region. The polymerase chain reaction, which amplifies the target DNA between two specific primers, has been performed for the amplification of human insulin gene and simple one-step cloning of it into Escherichia coli. Out of 1727 nuceotides compared, only 4 sites were variable: 5'-regulatory region(G2101$\rightarrow$AGG); IVS I(T2401$\rightarrow$A); Exon II(C2411 deletion); IVS II(A2740 dejection). The variations at the G2101 and T2401 were the same as those found in one American allele. The other two variations were observed only in the specific Korean allele. And, the enzyme digestion patterns among normal, insulin dependent diabetes mellitus, and non-insulin dependent diabetes mellitus were the same. On the other hand, PCR method showed the possibility of the quickaccess for the polymorphic region in terms of the restriction fragment length of polymorphism.

• Korean Journal of Veterinary Service
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• v.13 no.1
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• pp.64-68
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• 1990

This experiment was to investigate the Leptospiral antibody in the pigs with the serological test in two areas of Kangwondo from March to April. 1989. Twelve different living antigen (L. icterohaemorrhagiae, L. pomona, L. hardjo, L. australis, L. canicola, L. autumnalis, L. grippotyphosa, L. tarassovi, L. pyrogenes, L. bataviae, L. lai and L. ballum) of Leptospira interrogans was used for the serological test in the pigs. The blood sample of 280 pigs collected from the slaughter houses were detected by microscopic-agg-lutination test. The results were as follows, 1. Among the serum sample of 280 heads of the pigs, 6 heads of the swine (2.14%) were positive. 2. Among the positive sample of 6 heads, 3 heads of the swine (1.1%) showed the antibody of L. ict-erohaemorrhagiae, 2 heads of the swine (0.7%) showed the antibody of L. canicola, and 1 head of the swine (0.4%) showed the antibody of L. pomona. 3. The positive rate of Leptospira interrogans in the swine of Kangwondo appeared to be very low.

• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.942-945
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• 2008

There have been concerns about possible pathogenicity and antimicrobial resistance in Enterococcus, which constitute more than 50% of probiotics in the worldwide market. In this study, Enterococcus in sixteen products manufactured by ten different companies was tested for the presence of six virulence genes and two vancomycin resistance genes. Results in this study showed the safety of Enterococcus on the Korean market and the importance of screening vanA, vanE, agg, cylA, esp, and gelE. Pulse-field gel electrophoresis showed that the sixteen isolates tested in this study are originated from three strains.

• The Journal of the Korea Contents Association
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• v.20 no.3
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• pp.336-344
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• 2020

The purpose of this study was to investigate the PAI profile of the beauty major students. They were used through the PAI inspection result of 298 university students located in the chungcheong province in 2019. The results are summerized as follows; First, when comparing the average scale of college students majoring in beauty with those of college students majoring in general, college students majoring in beauty displayed a higher profile type in clinical scale than those of university students in general, with significant differences in SOM and ALC. Second, SOM, PAR, AGG, SUI that were significant when comparing beauty majors and general majors to navigate the PAI scale that significantly predicts beauty majors. As a result of conducting stepwise regression analysis on the four factors as a search factor, the scale of SOM is extracted, and it becomes a diversion that can predict college students in the beauty major. Therefore, beauty majors are more obsessed with physical problems than the ordinary students, and have an impulse-oriented behavior. These results will be used as materials for selecting, managing, educating or consulting a student majoring in beauty and will provide many implications for career choices after graduation.

• Journal of Life Science
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• v.21 no.1
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• pp.155-158
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• 2011

Uncoupling protein (UCP) 3 has a number of proposed roles in the regulation of fatty acid metabolism. A number of polymorphisms in the human UCP3 gene have been identified, and the correlation with obesity related phenotypes evaluated. The objective of this study was to identify SNP in porcine UCP3 gene and to investigate the effect of the SNP on economic traits. The sequencing analysis method was used to identify nucleotide polymorphisms at position 1405 bp (Genebank accession No : AY739704) in porcine UCP3 gene. The SNP (G150R), located in the exon 3, changed the amino acid to glycine (GGG) from arginine (AGG). This G150R showed three genotypes - GG, GR and RR - by digestion with the restriction enzyme Sma Ⅰ using the PCR-RFLP method. The G150R showed significant effects only on back fat (P<0.05). Animals with the genotype GG had significantly higher back fat thickness (1.358 cm) than animals with the genotype GR (1.288 cm, P<0.05) and RR (1.286 cm, P<0.05). However, the genotypes had no significant association with ADG and days to 90kg. According to results of this study, a G allele of the G150R was found to have a significant effect on back fat thickness. It will be possible to use SNP markers on selected pigs to improve backfat thickness, an important economic trait.

• Korean Journal of Psychosomatic Medicine
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• v.23 no.1
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• pp.12-19
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• 2015

Objectives : This study examined the characteristics and differences of PAI(Personality Assessment Inventory) profile between compensation-seeking(CS) and treatment-seeking(TS) patients with traumatic brain injury(TBI) and assessed the clinical meaning of the characteristics and differences of profiles between the two groups. Methods : 36 TBI patients who visited the Wonkwang University Hospital were selected. The patients were categorized as compensation-seeking TBI patients(n=22) and treatment-seeking TBI patients(n=14). The PAI scales and subscales were used to compare differences between two groups. t-verification for each variable and comparison analysis were performed. Results:In validity scales, CS group showed significantly higher NIM scores and lower PIM scores than TS groups. In full scales, CS group showed significantly higher SOM, ANX, ARD, DEP, and SCZ scores than TS group. In subscales, CS group showed significantly higher SOM-S, ANX-A, ARD-P, DEP(-C, A, P), (MAN-I), PAR-H, SCZ(-T, P), BOR(-A, N), and ANT-S scores than TS groups. In supplementary scales, CS group showed significantly higher SUI, NON and AGG-P, and lower RXR scores than TS group. Conclusions:There were significant differences in PAI scales with validity scales, some full and subscales according to compensation seeking status in TBI patients. The CS patients tended to exaggerate their symptoms on PAI, and showed higher scores representing somatic preoccupation and emotional distress. These results show the usefulness of PAI in reflecting the significant psychological differences between two groups.

• Korean Journal of School Psychology
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• v.16 no.3
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• pp.315-337
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• 2019

This study was intended to verify that the Revised PAI-A scale reflected the internalization and externalization classification of adolescence problem behavior. For this purpose, exploratory factor analysis and confirmatory factor analysis were conducted using PAI-A restandardization data. In addition, 31 Revised PAI-A sub-scales and SUI scale were used to identify the detailed factor structures. As a result of the analysis, the classification of internalization and externalization factors was similar to that of previous studies. In detail, the sub-scales of ANX·DEP and SUI were classified into internalization, the sub-scales of ANT and AGG were classified into externalization. It is noteworthy that each sub-scale of PAI-A was separated into internalization or externalization. For example, BOR-A, BOR-I, and BOR-N were loaded into internalization, but BOR-S into externalization. Next, in order to confirm whether the structure of the derived internalization and externalization factors can be applied to new samples, 350 samples were randomly extracted and confirmatory factor analysis was conducted, but exclusive of the samples used for exploratory factor analysis. As a result of confirmatory factor analysis, the appropriate indices of internalization and externalization classification was close to the good level. Therefore, the Revised PAI-A scales have theoretical relevance to internalization and externalization classification of problem behaviors. Based on the results of this study, it is expected that it can be used helpfully in the school settings in the future. Finally, the significance and limitations of this study were discussed.

• Journal of the Korean Ceramic Society
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• v.41 no.2
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• pp.131-135
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• 2004

The effect of Co additive on the microstructural evolusion of WC was investigated. A small amount of Co powder was placed on the top-center of the pure WC powder compact and then sintered at 1950$^{\circ}C$. During sintering some abnormally large WC grains of different size and shape observed depending on the distance from the liquid source. However, in the region far away from Co liquid source, it showed low densification and the grains of WC were very small and uniform in size. A small amount of Co liquid phase has a remarkable influence on the AGG of WC and it has been explained in terms of 2-D nucleation and growth mechanism.