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Roughage Energy and Degradability Estimation with Aspergillus oryzae Inclusion Using Daisy In vitro Fermentation

  • Chen, C.R.;Yu, B.;Chiou, P.W.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.1
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    • pp.53-62
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    • 2004
  • The aim of this study was to predict the energy value and dynamic degradation of roughage in Taiwan using the $Daisy^{(R)}$. in vitro fermentation method to provide information on one of the very important nutrients for ration formulation. The second objective was to study the effects of Aspergillus oryzae (AFE) inclusion on nutrient utilization. Three ruminal fistulated dry dairy cows were used for rumen fluid and fifteen conventional forages used in dairy cattle were collected around this island. The degradability of these feedstuffs with and without AFE ($Amaferm^{(R)}$.) treatment was measured using the $Daisy^{(R)}$. in vitro method. The roughage energy values, including TDN and NEL, were calculated according to Robinson (2000). Results from the 30 h in vitro neutral detergent fiber (NDF) degradability and predicted energy evaluations showed that alfalfa (among the forages) contained the highest degradability and energy values, Bermuda straw having the lowest. Peanut vines and corn silage contained higher energy values and the lowest value found in Pangola and Napier grasses among the locally produced forages. Pangola and Napier grasses had lower values than most imported forages except Bermuda straw. Among the by-products, wheat middling contained the highest NDF degradability, while rice bran contained the richest energy value due to its high oil content. From the dynamic dry matter (DM), organic matter (OM), acid detergent fiber (ADF) and neutral detergent fiber (NDF) degradation, corn silage contained the highest effective degradation among the local forages; wheat middling (among the by-products) degraded the fastest in DM, OM, ADF and NDF and showed the highest effective degradability. AFE inclusion was inconsistent among the forages. Alfalfa hay showed significantly increased 30 h NDF degradability and energy values, Pangola hay, Napier grass and brewer's grains showed decreased degradability and energy values. AFE inclusion increased the DM, OM and NDF degradation rate in most forage, but only increased the DM degradation rate in sorghum distiller's grains, the OM degradation rate in bean curd pomace and the NDF and ADF degradation rates in soy pomace (among the by-products).

Chemical Composition, Phenolic Concentration and In Vitro Gas Production Characteristics of Selected Acacia Fruits and Leaves

  • Abdulrazak, S.A.;Orden, E.A.;Ichinohe, T.;Fujihara, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.7
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    • pp.935-940
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    • 2000
  • The objective of this study was to evaluate the nutritive value of selected fruits (pods and seeds) and leaves of acacia tree species namely; Acacia nubica (nubica), Acacia tortilis (tortilis) and Acacia brevispica (brevispica), Acacia reficiens (reficiens) and Acacia senegal (senegal). A wide variability in chemical composition, polyphenolics and gas production was recorded. The crude protein (CP) ranged from 131 to 238 g/kg DM. Neutral detergent fiber (NDF), acid detergent fiber (ADF) and lignin (ADL) were higher in senegal and significantly different (p<0.05) from other species. The nitrogen bound to fiber tended to be higher in leaves than the fruits, ranging from 2.6 to 11.3 g/kg NDF and 1.6 to 3.2 g/kg ADF. The leaves of reficiens and senegal had higher concentrations of total extractable phenolics (TEPH), total extractable tannins (TET) and total condensed tannins (TCT), but lower in NDF, ADF and ADL than the fruits of nubica, tortilis and brevispica. Mineral concentrations varied among species; all were relatively poor in phosphorus, moderate in calcium and magnesium, and rich in microelements. A significant (p<0.05) variation in gas production after 12, 24, 48, 72 and 96 h was recorded between species. Nubica had the highest (p<0.05) rate of gas production (0.0925) while the highest potential gas production was recorded in tortilis. A strong negative correlation between TEPH and TET with gas production after 24, 48, 72 and 96 was established (r=-0.72 to -0.82). Crude protein and TCT correlated negatively but also weakly with gas production characteristics. Organic matter digestibility calculated from gas production after 48 h (OMD48) ranged between 465 g/kg DM in reficiens and 611 g/kg DM in tortilis. The results of this study indicate that acacia species have the potential to be used as feed supplements.

AMOUNT AND DISTRIBUTION OF DIETARY MINERALS IN SELECTED PHILIPPINE FORAGES

  • Serra, S.D.;Serra, A.B.;Ichinohe, T.;Harumoto, T.;Fujihara, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.9 no.2
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    • pp.139-147
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    • 1996
  • Eight Philoppine forages were studied to obtain the following: 1) nutrient concentrations and digestibility, 2) distribution of the various minerals in fiber fractions through mineral analyses of neutral detergent fiber(NDF) and acid detergent fiber(ADF) residues, and 3) correlation coefficients among the factors affecting forage quality and mineral concentrations. These Philippine forages were paragrass [Brachiaria mutica (Forsk.) Stapf], stargrass (Cynodon plectostachyum Pilger), napiergrass (Pennisetum purpureum Schumach.) calopo (Calopogonium muconoides Desv.), centrocema (Centrocema pubescens Benth.), gliricidia [Gliricidia sepium (Jacq.) Walp.] leucaena [Leucaena leucocephala (Lam.) de Wit.] and sesbania [Sesbania grandiflora (L.) Poir]. Species differences(p<0.01) were observed on various nutrient fractions including mineral composition and digestibility. The cell wall(NDF) fraction, prepared by boiling in neutral detergent solution, contained the following proportions of the total mineral originally present (%): calcium (Ca), 0.7; phosphorus(P), 14.3; magnesium(Mg), 1.9; potassium(K), 3.7; copper(Cu), 16.4; zinc(Zn), 2.9; molybdenum(Mo), 9.3; cobalt(Co), 16.2; manganese(Mn), 5.6, and iron(Fe), 81.3. The ligno-cellulose(ADF) fraction, prepared by boiling in acid detergent solution, contained the following proportions of the total mineral originally present(%): Ca, 0.2; P, 4.4; Mg, 0.7; K, 2.8; Cu, 32.3; Zn, 1.1; Mo, 8.9; Co, 4.7; Mn, 5.4; and Fe, 36.8. Correlation coefficients among the factors affection forage quality and mineral concentrations were also observed. Evidently, 75 and 45% of the minerals in grasses and legumes was positively correlated to CP and IVDMD, respectively. Moreover, 55, 80 and 75% of the forage minerals was negatively correlated to NDF, ADF and ADL fraction, respetively, implying that most of the minerals reside in the non-structural cell components.

Neutral detergent fiber rather than other dietary fiber types as an independent variable increases the accuracy of prediction equation for digestible energy in feeds for growing pigs

  • Choi, Hyunjun;Sung, Jung Yeol;Kim, Beob Gyun
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.4
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    • pp.615-622
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    • 2020
  • Objective: The objectives were to investigate correlations between energy digestibility (digestible energy [DE]:gross energy [GE]) and various fiber types including crude fiber (CF), total dietary fiber (TDF), soluble dietary fiber (SDF), insoluble dietary fiber (IDF), neutral detergent fiber (NDF), and acid detergent fiber (ADF), and to develop prediction equations for estimating DE in feed ingredients and diets for growing pigs. Methods: A total of 289 data with DE values and chemical composition of feeds from 39 studies were used to develop prediction equations for DE. The equations were validated using values provided by the National Research Council. Results: The DE values in feed ingredients ranged from 2,011 to 4,590 kcal/kg dry matter (DM) and those in diets ranged from 2,801 to 4,203 kcal/kg DM. In feed ingredients, DE:GE was negatively correlated (p<0.001) with NDF (r = -0.84), IDF (r = -0.83), TDF (r = -0.82), ADF (r = -0.78), and CF (r = -0.72). A best-fitting model for DE (kcal/kg) in feed ingredients was: 1,356 + (0.704 × GE, kcal/kg) - (60.3 × ash, %) - (27.7 × NDF, %) with R2 = 0.80 and p<0.001. In diets, DE:GE was negatively correlated (p<0.01) with NDF (r = -0.72), IDF (r = -0.61), TDF (r = -0.52), CF (r = -0.45), and ADF (r = -0.34). A best-fitting model for DE (kcal/kg) in diets was: 1,551 + (0.606 × GE, kcal/kg) - (22.1 × ash, %) - (25.6 × NDF, %) with R2 = 0.62 and p<0.001. All variables are expressed as DM basis. The equation developed for DE in feed ingredients had greater accuracy than a published equation for DE. Conclusion: All fiber types are reasonably good independent variables for predicting DE of swine feeds. The best-fitting model for predicting DE of feeds employed neutral detergent fiber as an independent variable.

Effect of Cultivar , Harvest Date and Drying Method on the Quality of Spring Harvest Oat Hay (품종 , 수확시기 및 건조제처리가 춘계수확 연맥건초의 사료가치에 미치는 영향)

  • 한건준;김동암
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.16 no.2
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    • pp.161-168
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    • 1996
  • This experiment was canied out to determine the effect of cultivar, harvest date and drying method on the changes of moisture content and quality of spring harvest oat(Avena sativa L.) hay. Disign of the experiment was a spilt-split-plot arrangement with early and late cultivars as main plots, early and late harvest dates as sub-plots, and drying agent and tedding as sub-subplots in three replications. Moisture content of early harvest oat was higher than that of late harvest oat by 127.1% dry weight(DW) in 'Swan' and 39.8%DW in 'Foothill'. Moisture content difference between 'Foothill' and 'Swan' was 117.6%DW at early and 204.9%DW at late harvest dates, respectively. Cultivar effect on moisture content of cut oat was higher at late harvest date, but drying method effect was higher at early harvest. Total nitrogen content of early harvest was higher than that of late harvest by 0.70% and that of 'Foothill' was higher than that of 'Swon' by 0.86%. But no difference in total nitrogen content was found between the drying method. ADF and NDF contents were increased at late harvest by 29% and 1 I%, respectively(P<0.05). ADF and NDF contents of 'Swan' were higher than those of 'Foothill' by 2.8% and 4.5%, respectively. No difference in ADF and NDF contents was obtained by drying methods. RFV of early harvest oat hay was 95.1 and 103.8 for 'Swan' and 'Foothill' but late harvest 'Swan' and 'Foothill' showed 74.8 and 85.0, respectively.

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Effect of Phosphate Fertilization and Cutting Management on Nitrogen Fixation and Feeding Value of Alfalfa ( Medicago sativa L. ) (인산시용 및 예취관리가 알팔파의 질소고정과 사료가치에 미치는 영향)

  • 이효원;김창호
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.17 no.4
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    • pp.371-378
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    • 1997
  • The purpose of this research was to investigate the effect of P fertilization and cutting fkquency without N fertilizer on nutritive value and nitrogen fixation activity of alfalfa. A field experiment was conducted on the experimental farm of Kongju National University at Yesan from 1994 to 1996. The experimental design was a split plot design with two P levels as main plot and two cutting frequencies as the sub-plot. The results obtained are summarized as follows. Available phosphate, exchangeable Ca and Na in the soil were slightly increased with P fertilizer and basal treatment of lime. Average DM yield was 1.5 ton and 1.4 ton in second and third year. There was no significant difference within P level and cutting kquency. The first cut showed higest CP value as cutting times increased the value tend to be lowered. Crude protein content in 3 and 4 cutting regimes were from 16.3 to 16.6% 17.4 to 17.9% respectively, but no significant effect were found among the main and sub-plot. ADF and NDF in 1995 were ranged from 38.2 to 39.9, 45.1 to 58.1% respectively. ADF was 2% more higher in 1996 compared to previous year and NDF was slightly higher in 1996. There was significant difference of ADF between main plot in 1995. .P, Ca, K and Mg of 200kg P level with 3 times cutting treatment in 1995 and 1996 were 0.32 and 0.23, 0.95 and 0.84, 3.24 and 2.87, and 0.36 and 0.26. Significant difference of K and Mg between cutting frequency was observed in 1995. Nitrogen fixing activity of 400kg P fertilizer plots were higher than 200kg P level and 3 times cutting plot also showed higher acetylene reduction activity than that of 4 cutting per year. Phosphate level and cutting frequency treatment of did not significantly affect nitrogen fixation activity.

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OPTICAL SURVEY WITH KMTNET FOR DUSTY STAR-FORMING GALAXIES IN THE AKARI DEEP FIELD SOUTH

  • JEONG, WOONG-SEOB;KO, KYEONGYEON;KIM, MINJIN;KO, JONGWAN;KIM, SAM;PYO, JEONGHYUN;KIM, SEONG JIN;KIM, TAEHYUN;SEO, HYUN JONG;PARK, WON-KEE;PARK, SUNG-JOON;KIM, MIN GYU;KIM, DONG JIN;CHA, SANG-MOK;LEE, YONGSEOK;LEE, CHUNG-UK;KIM, SEUNG-LEE;MATSUURA, SHUJI;PEARSON, CHRIS;MATSUHARA, HIDEO
    • Journal of The Korean Astronomical Society
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    • v.49 no.5
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    • pp.225-232
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    • 2016
  • We present an optical imaging survey of AKARI Deep Field South (ADF-S) using the Korea Microlensing Telescope Network (KMTNet), to find optical counterparts of dusty star-forming galaxies. The ADF-S is a deep far-infrared imaging survey region with AKARI covering around 12 deg2, where the deep optical imaging data are not yet available. By utilizing the wide-field capability of the KMTNet telescopes (~4 deg2), we obtain optical images in B, R and I bands for three regions. The target depth of images in B, R and I bands is ~24 mag (AB) at 5σ, which enables us to detect most dusty star-forming galaxies discovered by AKARI in the ADF-S. Those optical datasets will be helpful to constrain optical spectral energy distributions as well as to identify rare types of dusty star-forming galaxies such as dust-obscured galaxy, sub-millimeter galaxy at high redshift.

Studies on Feed Resources of Non-Conventional Agriculture Roughage (농업부산물 사료화 이용 연구)

  • Kim, Weon-Ho;Seo, Sung;Park, Geun-Je;Shin, Dong-Eun;Choi, Gee-Jun;Kim, Kee-Yong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.21 no.3
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    • pp.169-174
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    • 2001
  • This study was conducted to investigate the effects of feed resources and collection system of nonconventional agricultural roughages, and reduction of environment pollution by turning non-conventional agricultural roughages to feed resources and diversification of feed supply. And a series of experiment were conducted to determine the effect of moisture control material on quality of sweet potato stalk, peanut stalk, barley straw, the experiments were conducted at Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwon in 1999 and 2000. The results obtained from these experiments are summarized as follows; Acid detergent fiber(ADF), neutral detergent fiber(NDF) and total digestible nutrient(TDN) of sweet potato were 38.8, 50.7 and 58.2% in the control silage, but that of rice straw added silage for moisture control were decreased to 41.5, 50.8 and 56.1%. ADF, NDF, CP(crude protein) and TDN of peanut stalk were 42.2, 49.6, 10.5 and 55.6% on the rice straw added silage for moisture control, and there were not affected by barely straw added silage for moisture control. And ADF, NDF and TDN content of barely straw increased 42.8, 65.8 and 55.1% on the water+inoculants treated silage. The results of this study indicate that non-conventional agricultural roughages to feed resources should be make for silage at silo after dry matter percentage(ab0ut 35%) control. Also, rice straw added silage for moisture control will improve the silage fermentation and conservation. (Key words : Feed resources, Non-conventional agriculture, Fermentation, Inoculant, Rice straw)

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Prediction of Covid-19 confirmed number of cases using SARIMA model (SARIMA모형을 이용한 코로나19 확진자수 예측)

  • Kim, Jae-Ho;Kim, Jang-Young
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.26 no.1
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    • pp.58-63
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    • 2022
  • The daily number of confirmed cases of Coronavirus disease 2019(COVID-19) ranges between 1,000 and 2,000. Despite higher vaccination rates, the number of confirmed cases continues to increase. The Mu variant of COVID-19 reported in some countries by WHO has been identified in Korea. In this study, we predicted the number of confirmed COVID-19 cases in Korea using the SARIMA for the Covid-19 prevention strategy. Trends and seasonality were observed in the data, and the ADF Test and KPSS Test was used accordingly. Order determination of the SARIMA(p,d,q)(P, D, Q, S) model helped in extracting the values of p, d, q, P, D, and Q parameters. After deducing the p and q parameters using ACF and PACF, the data were transformed and schematized into stationary forms through difference, log transformation, and seasonality removal. If seasonality appears, first determine S, then SARIMA P, D, Q, and finally determine ARIMA p, d, q using ACF and PACF for the order excluding seasonality.

Expression and Functional Analysis of cofilin1-like in Craniofacial Development in Zebrafish

  • Jin, Sil;Jeon, Haewon;Choe, Chong Pyo
    • Development and Reproduction
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    • v.26 no.1
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    • pp.23-36
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    • 2022
  • Pharyngeal pouches, a series of outgrowths of the pharyngeal endoderm, are a key epithelial structure governing facial skeleton development in vertebrates. Pouch formation is achieved through collective cell migration and rearrangement of pouch-forming cells controlled by actin cytoskeleton dynamics. While essential transcription factors and signaling molecules have been identified in pouch formation, regulators of actin cytoskeleton dynamics have not been reported yet in any vertebrates. Cofilin1-like (Cfl1l) is a fish-specific member of the Actin-depolymerizing factor (ADF)/Cofilin family, a critical regulator of actin cytoskeleton dynamics in eukaryotic cells. Here, we report the expression and function of cfl1l in pouch development in zebrafish. We first showed that fish cfl1l might be an ortholog of vertebrate adf, based on phylogenetic analysis of vertebrate adf and cfl genes. During pouch formation, cfl1l was expressed sequentially in the developing pouches but not in the posterior cell mass in which future pouch-forming cells are present. However, pouches, as well as facial cartilages whose development is dependent upon pouch formation, were unaffected by loss-of-function mutations in cfl1l. Although it could not be completely ruled out a possibility of a genetic redundancy of Cfl1l with other Cfls, our results suggest that the cfl1l expression in the developing pouches might be dispensable for regulating actin cytoskeleton dynamics in pouch-forming cells.