• 한국자기공명학회논문지
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• 제21권3호
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• pp.90-95
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• 2017

The Z-DNA domain of human ADAR1 ($Z{\alpha}_{ADAR1}$) produces B-Z junction DNA through preferential binding to the CG-repeat segment and destabilizing the neighboring AT-rich region. However, this study could not answer the question of how many base-pairs in AT-rich region are destabilized by binding of $Z{\alpha}_{ADAR1}$. Thus, we have performed NMR experiments of $Z{\alpha}_{ADAR1}$ to the longer DNA duplex containing an 8-base-paired (8-bp) CG-repeat segment and a 12-bp AT-rich region. This study revealed that $Z{\alpha}_{ADAR1}$ preferentially binds to the CG-repeat segment rather than AT-rich region in a long DNA and then destabilizes at least 6 base-pairs in the neighboring AT-rich region for efficient B-Z transition of the CG-repeat segment.

• Molecules and Cells
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• 제46권6호
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• pp.351-359
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• 2023

Deamination of adenine or cytosine in RNA, called RNA editing, is a constitutively active and common modification. The primary role of RNA editing is tagging RNA right after its synthesis so that the endogenous RNA is recognized as self and distinguished from exogenous RNA, such as viral RNA. In addition to this primary function, the direct or indirect effects on gene expression can be utilized in cancer where a high level of RNA editing activity persists. This report identified actin-related protein 2/3 complex inhibitor (ARPIN) as a target of ADAR1 in breast cancer cells. Our comparative RNA sequencing analysis in MCF7 cells revealed that the expression of ARPIN was decreased upon ADAR1 depletion with altered editing on its 3'UTR. However, the expression changes of ARPIN were not dependent on 3'UTR editing but relied on three microRNAs acting on ARPIN. As a result, we found that the migration and invasion of cancer cells were profoundly increased by ADAR1 depletion, and this cellular phenotype was reversed by the exogenous ARPIN expression. Altogether, our data suggest that ADAR1 suppresses breast cancer cell mobility via the upregulation of ARPIN.

• Bulletin of the Korean Chemical Society
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• 제32권2호
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• pp.719-721
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• 2011

• BMB Reports
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• 제53권9호
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• pp.453-457
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• 2020

The right-handed double-helical structure of DNA (B-DNA), which follows the Watson-Crick model, is the canonical form of DNA existing in normal physiological settings. Even though an alternative left-handed structure of DNA (Z-DNA) was discovered in the late 1970s, Z-form nucleic acid has not received much attention from biologists, because it is extremely unstable under physiological conditions, has an ill-defined mechanism of its formation, and has obscure biological functions. The debate about the physiological relevance of Z-DNA was settled only after a class of proteins was found to potentially recognize the Z-form architecture of DNA. Interestingly, these Z-DNA binding proteins can bind not only the left-handed form of DNA but also the equivalent structure of RNA (Z-RNA). The Z-DNA/RNA binding proteins present from viruses to humans function as important regulators of biological processes. In particular, the proteins ADAR1 and ZBP1 are currently being extensively re-evaluated in the field to understand potential roles of the noncanonical Z-conformation of nucleic acids in host immune responses and human disease. Despite a growing body of evidence supporting the biological importance of Z-DNA/RNA, there remain many unanswered principal questions, such as when Z-form nucleic acids arise and how they signal to downstream pathways. Understanding Z-DNA/RNA and the sensors in different pathophysiological conditions will widen our view on the regulation of immune responses and open a new door of opportunity to develop novel types of immunomodulatory therapeutic possibilities.

• Molecules and Cells
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• 제46권11호
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• pp.725-725
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• 2023

• Bulletin of the Korean Chemical Society
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• 제35권12호
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• pp.3655-3657
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• 2014

• 보건의료생명과학 논문지
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• 제8권2호
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• pp.121-125
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• 2020

인원계수는 특정 시설의 혼잡도나 이용량을 파악하여 시설을 효율적으로 관리하거나 화장실 등 특정 장소의 출입인원을 파악하여 자동으로 절전기능을 구현함으로써 에너지 절약을 최대화하기 위한 용도로 늘 관심있는 방법이었다. 영상처리에 의한 인원계수 방법은 매우 비용이 비싸며 조명의 주위환경에 심하게 영향을 받는 단점이 있고 area sensor의 경우 인원이 팔짱을 끼고 근접하여 통과할 경우 1명의 인원으로 계수하는 단점이 있다. 비용이 비싸고 조명에 영향을 받거나 특정한 경우 인원계수가 부정확한 기존의 방법을 해결하기 위하여 본 논문에서는 라이다(LIADAR)의 원리를 이용한 새로운 인원계수 방법을 제안한다. 병원 출입인원을 정확하기 계수하는 것은 병원시설을 관리하는 데도 도움이 되겠지만 코로나19가 만연한 현시점에서 효율적인 방역대책을 세우는 데도 도움이 될 것이다.