• 제목/요약/키워드: ADAMTS13 protein

검색결과 11건 처리시간 0.016초

Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo

  • Park, Jin Sung;Lee, Hyun Jae;Lee, Dong Yeong;Jo, Ho Seung;Jeong, Jin Hoon;Kim, Dong Hee;Nam, Dae Cheol;Lee, Choong Jae;Hwang, Sun-Chul
    • Biomolecules & Therapeutics
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    • 제23권5호
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    • pp.442-448
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    • 2015
  • We evaluated the chondroprotective effects of wogonin by investigating its effects on the gene expression and production of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as on production of MMP-3 in the rat knee. Rabbit articular chondrocytes were cultured in a monolayer, and RT-PCR was used to measure interleukin-$1{\beta}$ (IL-$1{\beta}$)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and type II collagen. In rabbit articular chondrocytes, the effects of wogonin on IL-$1{\beta}$-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The effect of wogonin on MMP-3 protein production was also examined in vivo. In rabbit articular chondrocytes, wogonin inhibited the expression of MMP-3, MMP-1, MMP-13, and ADAMTS-4, but increased expression of type II collagen. Furthermore, wogonin inhibited the production and proteolytic activity of MMP-3 in vitro, and inhibited production of MMP-3 protein in vivo. These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes.