• Journal of Pharmacopuncture
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• v.19 no.3
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• pp.213-224
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• 2016

Objectives: Ginseng Rh2+ is enzyme-treated ginseng extract containing high amounts of converted ginsenosides, such as compound k, Rh2, Rg3, which have potent anticancer activity. We conducted general and genetic toxicity tests to evaluate the safety of ginseng Rh2+. Methods: An acute oral toxicity test was performed at a high-level dose of 4,000 mg/kg/day in Sprague-Dawley (SD) rats. A 14-day range-finding study was also conducted to set dose levels for the 90-day study. A subchronic 90-day toxicity study was performed at dose levels of 1,000 and 2,000 mg/kg/day to investigate the no-observed-adverse-effect level (NOAEL) of ginseng Rh2+ and target organs. To identify the mutagenic potential of ginseng Rh2+, we conducted a bacterial reverse mutation test (Ames test) using amino-acid-requiring strains of Salmonella typhimurium and Escherichia coli (E. coli), a chromosome aberration test with Chinese hamster lung (CHL) cells, and an in vivo micronucleus test using ICR mice bone marrow as recommended by the Korean Ministry of Food and Drug Safety. Results: According to the results of the acute oral toxicity study, the approximate lethal dose (ALD) of ginseng Rh2+ was estimated to be higher than 4,000 mg/kg. For the 90-day study, no toxicological effect of ginseng Rh2+ was observed in body-weight changes, food consumption, clinical signs, organ weights, histopathology, ophthalmology, and clinical pathology. The NOAEL of ginseng Rh2+ was established to be 2,000 mg/kg/day, and no target organ was found in this test. In addition, no evidence of mutagenicity was found either on the in vitro genotoxicity tests, including the Ames test and the chromosome aberration test, or on the in vivo in mice bone marrow micronucleus test. Conclusion: On the basis of our findings, ginseng Rh2+ is a non-toxic material with no genotoxicity. We expect that ginseng Rh2+ may be used as a novel adjuvant anticancer agent that is safe for long-term administration.

• Environmental Analysis Health and Toxicology
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• v.28
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• pp.2.1-2.7
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• 2013

Objectives Tricalcium phosphate and calcium hydrogenorthophosphate are high production volume chemicals, mainly used as foodstuff additives, pharmaceuticals, lubricants, synthetic resin, and disinfectants. Phosphate has the potential to cause increased algal growth leading to eutrophication in the aquatic environment. However, there is no adequate information available on risk assessment or acute and chronic toxicity. The aim of this research is to evaluate the toxic potential of phosphate compounds in the aquatic environment. Methods An aquatic toxicity test of phosphate was conducted, and its physico-chemical properties were obtained from a database recommended in the Organization for Economic Cooperation and Development (OECD) guidance manual. An ecotoxicity test using fish, Daphnia, and algae was conducted by the good laboratory practice facility according to the OECD TG guidelines for testing of chemicals, to secure reliable data. Results The results of the ecotoxicity tests of tricalcium phosphate and calcium hydrogenorthophosphate are as follows: In an acute toxicity test with Oryzias latipes, 96 hr 50% lethal concentration ($LC_{50}$) was >100 (measured:>2.14) mg/L and >100 (measured: >13.5) mg/L, respectively. In the Daphnia test, 48 hr 50% effective concentration ($EC_{50}$) was >100 (measured: >5.35) mg/L and >100 (measured: >2.9) mg/L, respectively. In a growth inhibition test with Pseudokirchneriella subcapitata, 72 hr $EC_{50}$ was >100 (measured: >1.56) mg/L and >100 (measured: >4.4) mg/L, respectively. Conclusions Based on the results of the ecotoxicity test of phosphate using fish, Daphnia, and algae, $L(E)C_{50}$ was above 100 mg/L (nominal), indicating no toxicity. In general, the total phosphorus concentration including phosphate in rivers and lakes reaches levels of several ppm, suggesting that phosphate has no toxic effects. However, excessive inflow of phosphate into aquatic ecosystems has the potential to cause eutrophication due to algal growth.

• Korean Journal of Oriental Medicine
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• v.16 no.3
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• pp.149-154
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• 2010

Objective : Ssanghwa-tang has been used as herbal medine, favorite beverage or health beverage. This study was performed to evaluate the acute toxity and safety of fermented Ssanghwa-tang extract in ICR mice. Methods : 0(control group), 1250, 2500 and 5000 mg/kg of Ssanghwa-tang and fermented Ssanghwa-tang extracts were orally administered to 35 male and 35 female ICR mice. After single administration, we observed number of death, clinical signs, changes of body weight for 14 days. After 14 day of administration, all mice were sacrificed and major organ were observed. Results : Compared with the control group, we could not find any toxic alteration in all treated groups (1250, 2500 and 5000 mg/kg). Conclusions : These results suggest that Sssanghwa-tang fermented with nuruk extracts might be safe to ICR mice.

• Journal of Food Hygiene and Safety
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• v.11 no.4
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• pp.299-306
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• 1996

6-[(N-3,4-Dibromophenyl)amino]-7-chloro-5,8-quinolinedione(FCK13) was tested for antifungal activities. The MIC values were determined by the two-fold dilution method. The therapeutic potential of RCK13 had been assessed in comparison with ketoconazole and fluconazole against systemic infections with candida albicans in normal mice. RCK13 had ED50,0.80$\pm$0.21 mg/kg but ketoconazole had ED50, 8.00$\pm$0.73 mg/kg respectively. And administered RCK13 at the ED50 for 14 days improved survival rates as well as ketoconazole. Acute oral toxicity studies of RCK13 were carried out in ICR mice of both sexes. These acute oral toxicities of RCK13 were low and LD50 values were over 2,850 mg/kg in ICR mice. The genotoxicities of RCK13 had been evaluated. RCK13 was negative in Ames test with Salmonella typhimurium and chromosomal aberration test in CHL cells. The clastogenicity was tested on the RCK13 with in vivo mouse micronucleus assay. RCK13 did not show any clastogenic effect in mouse peripheral blood and was negative in mouse micronucleus assay. These results indicate that RCK13 has no genotoxic potential under these experimental conditions.

• Korean Journal of Pharmacognosy
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• v.17 no.3
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• pp.242-247
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• 1986

This study is an attempt to investigate the effects of the water extracts of the whole plants of Prunella vulgaris (Labiatae) and Thesii chinense (Santalaceae) on the acute toxicity, the activities on central nervous system and the diuretic action. The acute toxicities shown by $LD_{50}$ were estimated to be more than 3000 mg/kg p.o. and 1,000 mg/kg s.c. in the extracts of Prunellae Herba and Thesii Herba, respectively, in mice. The extracts at a dose of 2,000 mg/kg p.o. did not show any activities on central nervous system, i.e, sedative, analgesic, hypothermic and anticonvulsant actions. The urination in rats was increased by 45.5% and 57.6% when 100mg/kg of each of the extracts were given orally. The results obtained revealed that the water extracts possessed weak diuretic actions without any of central nervous system activities. Furthermore, it is considered that the potassium in the extract may play a role in the diuretic action.

• Journal of Food Hygiene and Safety
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• v.14 no.1
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• pp.55-59
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• 1999

6-(4-Iodophenyl)amino-7-chloro-5,8-quinolinedione (RCK9) was evaluated for antifungal activities. The MIC values of RCK9 were determined against A. flavus, c. albicans, C. neoformans and F. oxysporium. The RCK9 showed generally potent antifungal activities against the tested fungi. Acute oral toxicity studies of RCK9 were carried out in ICR mice of both sexes. These acute oral toxicities of RCK9 had been evaluated. RCK9 were low and LD50 values were over 2,850 mg/kg in ICR mice. The genotoxicities of RCK9 had been evaluated. RCK9 was negative in Ames test with Salmonella typhimurium and chromosomal aberration test in CHL cells. The clastogenicity was tested on the RCK9 with in vivo mouse micronucleus assay. RCK9 did not show any clastogenic effect in mouse peripheral blood and was negative in mouse micronucleus assay. The results indicate that RCK9 has no genotoxic potential under these experimental conditions.

• Toxicological Research
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• v.12 no.2
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• pp.295-303
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• 1996

Acute toxic effects of the Woo Whang Chung Shim Won suspension and pills were studied with the doses of 60, 74, 90, and 110 ml/kg in mice. In this study, we daily examined number of deaths, clinical signs, body weights, and pathological findings for 7 days after administration of Woo Whang Chung Shim Won. All mice given the highest dose (110ml/kg) died at 24 hour after administration of Sam-Sung Woo Whang Chung Shim Won suspension (Byunbang, SS-BS and Wonbang, SS-WS), one male and two female mice given 90 ml/kg dose of SS-BS died, and a few mice given middle dose (74 ml/kg) of SS-WS died. In control group (110 ml/kg) treated with vehicle (SS-BS and SS-WS), three out of 5 males and two out of 5 females mice died during the study. However, in animals treated with Kwang-Dong Woo Whang Chung Shim Won suspension (KD-S) and pill (KD-P), deaths were not observed. In the clinical signs, increase of drooling and decrease of spontaneous motor activities were observed in the highest dose group (110 ml/kg). No significant dose-related change in body weight was observed. The results suggest that the toxic effect of SS-BS and SS-WS may be atttributed to the solution for the Woo Whang Chung Shim Won suspension.

• The Korean Journal of Pesticide Science
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• v.12 no.4
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• pp.382-390
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• 2008

This study was performed to evaluate the acute toxicity and residual toxicity of the 69 kinds of agrochemicals (41 insecticides, 18 fungicides, and 10 acaricides) against honeybee, Apis mellifera and bumblebee, Bombus terrestris. According to the IOBC standard, the toxicity showed below 30% was classified as non-toxic. Among 41 insecticides, five insecticides (acetamiprid, chlorfenapyr, thiacloprid, milbemectin, and buprofezin+amitraz) against the honeybee; eight insecticides (methomyl, thiodicarb, acetamiprid, chlorfenapyr, thiacloprid, abamectin, spino sad, buprofezin+amitraz) against the bumblebee did not show any toxic effect. Therefore, it thought to being safe. Other 18 fungicides and 10 acaricides were safe against the honeybee and bumblebee. In residual toxicity against the honeybee, eight insecticides (dichlorvos, methomyl, imidachlorprid, emamectin benzoate, spinosad, cartap hydrochloride, chlorfenapyr, and endosulfan) among 41 insecticides tested were safe at three days after treatment; however, sixteen insecticides (dimethoate, fenitrothion, fenthion, methidathion, phenthoate, pyraclofos, fenpropathrin, clothianidin, dinotefuran, thiamethoxam, abamectin, acetamiprid+ethofenprox, acetamiprid+indoxacarb, bifenthrin+imidacloprid, ethofenprox+phenthoate, imidacloprid+methiocarb) still remain high toxicity at eleven days after treatment. Against the bumblebee, residual toxicity showed as safe in seven insecticides (dimethoate, methidation, a-cypermethion, ethofenprox, indoxcarb, chlorpyrifos+a-cypennethrin, esfenvalerate+fenitrochion) at three days after treatment; however, eight insecticides (fenitrothion, pyraclofos, clothianidin, fipronil, acetamiprid+ethofenprox, chlorpyrifos+bifenthrin, ethofenprox+phenthoate, imidacloprid+methiocarb) still showed high toxicity at seven days after treatment. From above results, it will be useful information to select insecticides being safe and effective against the honeybee and bumblebee.

• The Korean Journal of Pesticide Science
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• v.21 no.1
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• pp.33-41
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• 2017

Susceptibility and persistence of Japanese pine sawyer, Monochamus alternatus adults, and acute contact toxicity, foliage contact toxicity, and residual toxicity to honeybee, Apis mellifera were evaluated by using 5 kinds of neonicotinoid pesticides. All neonicotinoids pesticides showed over 80% mortality 48 hour after body spray treatment. However, only 2 kinds of acetamiprid ME and acetamiprid+buprofezin showed over 80% mortality in the twig dipping treatment. All of the five neonicotinoides pesticides used in M. alternatus adults showed residual effect of 60-80% mortality, but the efficacy decreased rapidly after 1 day of treatment. Acetamiprid ME showed the lowest toxicity in the acute and foliage contact toxicity test of A. mellifera. The residual toxicity of leaves on A. mellifera was very low in acetamiprid ME and acetamiprid+buprofezin. However, the residual toxicity of all pesticides treatments decreased to 10-30% after 1 day of treatment and further decreased with time. These results indicate that acetamiprid ME among five neonicotinoid agents showed a high insecticidal activity in the M. alternatus adults, while it exhibited relatively low contact and residual toxicity in the A. mellifera. It is thought that acetamiprid ME can be effectively used for the control of the M. alternatus adults as vector of pine wilt disease.

• Journal of Korean Society on Water Environment
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• v.28 no.3
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• pp.467-472
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• 2012

In this study, water quality and acute toxicity using Daphnia magna were analyzed to investigate the eco-toxicological substances identified as statistical analysis and propose a management plan for the effluent of Banwol industrial complex, Ansan, Gyeonggi-do. Cu, Zn, F, Mn concentrations in the effluent far exceeded the US standards to protect the aquatic ecosystem and eco-toxicity values were 5 ~ 22 TU. However, concentrations of heavy metals significantly decreased after Ansan public wastewater treatment plant operating a biological treatment and toxicity values were 0 TU. Zn, Cr, F and Cu in the effluent showed very strong and strong positive correlations with eco-toxicity values, respectively. Regression analysis resulted in an equation between toxicity and Zn, TU = $4.884{\times}Zn$ (mg/L) -0.391 showing Zn concentration should be managed less than 0.285 mg/L to keep the eco-toxicity (TU) less than 1.