• 디지털콘텐츠학회 논문지
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• 제19권7호
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• pp.1263-1270
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• 2018

본 연구는 개발도상국 고등교육을 위한 이러닝 플랫폼에 대하여 연구하였다. 먼저 ICT기술환경 등 이러닝 환경요인과 개발도상국을 위한 아세안사이버대학(ACU) 프로젝트를 소개한 후, 개발도상국 이러닝 플랫폼 사례로서 캄보디아 사례를 제시하였다. 캄보디아의 국가교육정책 및 ICT환경, 현지의 이러닝 수요 및 환경요소를 분석하였으며, 분석 결과에 따른 이러닝 전략과 세부방안을 도출하고 이러닝 플랫폼 C-MOOC Net을 제안하였다. 제안된 방법을 검증하기 위하여 C-MOOC Net 시스템의 프로토타입을 공개소프트웨어 기반으로 개발하여 실제로 운영한 결과 C-MOOC 허브의 연계, 현지 언어의 지원, 선호강좌의 개인화 등록 등 요구사항을 충족함을 확인하였다.

• 대한의생명과학회지
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• 제13권3호
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• pp.239-244
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• 2007

Hoxc8 is one of the homeotic developmental control genes regulating the expression of many downstream target genes, through which animal body pattern is established during embryonic development. In previous proteomics analysis, proliferating cell nuclear antigen (PCNA) which is also known as cyclin, has been implied to be regulated by Hoxc8 in F9 murine embryonic teratocarcinoma cell. When the 5' upstream region of PCNA was analyzed, it turned out to contain 20 Hox core binding sites (ATTA) in about 1.17 kbp (kilo base pairs) region ($-520{\sim}-1690$). In order to test whether this region is responsible for Hoxc8 regulation, the upstream 2.3 kbp fragment of PCNA was amplified through PCR and then cloned into the pGL3 basic vector containing a luciferase gene as a reporter. When the luciferase activity was measured in the presence of effector plasmid (pcDNA : c8) expressing murine Hoxc8, the PCNA promoter driven reporter activity was reduced. To confirm whether this reduction is due to the Hoxc8 protein, the siRNA against Hoxc8 (5'-GUA UCA GAC CUU GGA ACU A-3' and 5'-UAG UUC CAA GGU CUG AUA C-3') was prepared. Interestingly enough, siRNA treatment up regulated the luciferase activity which was down regulated by Hoxc8, indicating that Hoxc8 indeed regulates the expression of PCNA, in particular, down regulation in NIN3T3 cells. These results altogether indicate that Hoxc8 might orchestrate the pattern formation by regulating PCNA which is one of the important proteins involved in several processes such as DNA replication and methylation, chromatin remodeling, cell cycle regulation, differentiation, as well as programmed cell death.