• Journal of Digital Contents Society
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• v.19 no.7
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• pp.1263-1270
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• 2018

We investigated the e-learning platforms for higher education in developing countries. First, we introduced e-learning environmental factors such as ICT technology as well as ASEAN Cyber University (ACU) project for developing countries. And then, we presented Cambodian case as an example of e-learning platform for developing countries. We analyzed the national education policy and ICT environment as well as the demands and obstacles of e-learning in Cambodia. We derived e-learning strategies and plans based on the analysis results, and finally proposed e-learning platform C-MOOC Net. In order to verify the proposed scheme, we developed prototype of C-MOOC Net system which is implemented based on open software. As a result, we have met the requirement of networking the C-MOOC hub, supporting localization of local language and personalization of favorite courses respectively.

• Biomedical Science Letters
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• v.13 no.3
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• pp.239-244
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• 2007

Hoxc8 is one of the homeotic developmental control genes regulating the expression of many downstream target genes, through which animal body pattern is established during embryonic development. In previous proteomics analysis, proliferating cell nuclear antigen (PCNA) which is also known as cyclin, has been implied to be regulated by Hoxc8 in F9 murine embryonic teratocarcinoma cell. When the 5' upstream region of PCNA was analyzed, it turned out to contain 20 Hox core binding sites (ATTA) in about 1.17 kbp (kilo base pairs) region ($-520{\sim}-1690$). In order to test whether this region is responsible for Hoxc8 regulation, the upstream 2.3 kbp fragment of PCNA was amplified through PCR and then cloned into the pGL3 basic vector containing a luciferase gene as a reporter. When the luciferase activity was measured in the presence of effector plasmid (pcDNA : c8) expressing murine Hoxc8, the PCNA promoter driven reporter activity was reduced. To confirm whether this reduction is due to the Hoxc8 protein, the siRNA against Hoxc8 (5'-GUA UCA GAC CUU GGA ACU A-3' and 5'-UAG UUC CAA GGU CUG AUA C-3') was prepared. Interestingly enough, siRNA treatment up regulated the luciferase activity which was down regulated by Hoxc8, indicating that Hoxc8 indeed regulates the expression of PCNA, in particular, down regulation in NIN3T3 cells. These results altogether indicate that Hoxc8 might orchestrate the pattern formation by regulating PCNA which is one of the important proteins involved in several processes such as DNA replication and methylation, chromatin remodeling, cell cycle regulation, differentiation, as well as programmed cell death.