• 제목/요약/키워드: AC7A

검색결과 876건 처리시간 0.028초

Expression of a Fusion Protein with Cry1Ac Protein and a Scorpion Insect Toxin in Acrystalliferous Bacillus thuringiensis Strain

  • Roh, Jong-Yul;Li, Ming-Shun;Chang, Jin-Hee;Park, Jae-Young;Shim, Hee-Jin;Shin, Sang-Chul;Boo, Kyung-Saeng;Je, Yeon-Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • 제8권1호
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    • pp.89-93
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    • 2004
  • Expression of a fusion protein between B. thuringiensis crystal protein, Cry1Ac1 and a scorpion insect toxin (AaIT, Androctonus australis Hector insect toxin) in acrystalliferous B. thuringiensis strain (Cry-B strain) was examined. The cry 1Ac1 gene was cloned in B. thuringiensis-E coli shuttle vector, pHT3101, under the control of the native cry 1Ac1 gene promoter (pProAc) and a gene encoding AaIT was inserted in XhoI site in the middle of the cry 1Ac1 gene (pProAc-ScoR). B. thuringiensis Cry-B strain carrying pProAc-ScoR (PyoAc-ScoR/CB) produced an inclusion body of irregular shape and the expressed fusion protein is approximately 65 kDa in size. Sporulated cells and spore-crystal mixtures of ProAc-ScoR/CB had insecticidal activity against Plutella xylostella larvae, showing $LT_50$ of ProAc-ScoR/CB (22.59 hrs) lower than that of ProAc/CB (30.06 hrs) at $1{\times}{10^7} {CEU/cm^2}$. These results suggest that the fusion protein including a B. thuringiensis crystal protein and an AaIT may be functionally expressed in B. thupingiensis. Moreover, we verified the additive toxicity of AaIT, which is a new feasible candidate for insect control.

망간산화물이 첨착된 활성탄에 의한 페놀흡착 및 비소(III) 산화 (As(III) Oxidation and Phenol Adsorption by the Activated Carbon Impregnated with Mn Oxide)

  • 유목련;양재규;장윤영
    • 대한환경공학회지
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    • 제30권4호
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    • pp.423-429
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    • 2008
  • 유기물과 무기물이 함께 오염되어 있는 합성 폐수처리에 대한 망간첨착활성탄(Mn-AC)의 적용성을 연구하였다. 유기물과 무기물의 대표물질로 페놀과 3가 비소를 각각 선정하였다. 산성조건에서 Mn-AC의 안정성을 평가하기 위해, 용액의 pH를 2, 3 및 4로 달리하여 시간경과에 따른 용존망간의 용출능을 평가하였을 때, pH 3보다 낮은 조건에서는 Mn-AC의 안정성이 떨어졌지만, pH 4에서는 반응시간동안 용출되는 망간의 농도는 무시할 수 있는 정도였다. 이러한 안정성 실험결과 Mn-AC는 pH 4 이상의 일반폐수 처리에 사용할 수 있음을 알 수 있었다. AC와 비교하였을 때, 망간첨착에 의한 비표면적 감소로 인해 망간첨착활성탄의 페놀제거속도 및 페놀 흡착능은 일부 감소하였다. 페놀 농도 변화에 따른 등온흡착결과 Mn-AC의 최대흡착량은 AC의 75%에 해당하였다. 산성영역의 pH에서는 Mn-AC에 의한 3가 비소 산화효율이 AC보다 높았지만 pH 7 이상에서는 반대 경향을 나타내었다. 본 연구를 통하여 Mn-AC를 페놀과 3가 비소의 동시처리에 적용할 수 있음을 알 수 있었다.

Production of the Eggs with Abnormal Shape from the Domestic Silkworm, Bombyx mori, Infected with Autographa californica Nuclear Polyhedrosis Virus

  • Lee, Sang-Mong;Park, Nam-Sook;Park, Hye-Jin;Yun, Eun-Young;Kang, Seok-Woo;Kim, Keun-Young;Sohn, Hung-Dae;Jin, Byung-Rae
    • International Journal of Industrial Entomology and Biomaterials
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    • 제1권2호
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    • pp.111-114
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    • 2000
  • The female pupae of the silkworms Bombyx mori, were injected with recombinant Autographa californica nuclear polyhedrosis virus (AcNPV) expressing green fluorescent protein (GFP) by percutaneous inoculation. When the 4 day-old female pupae were injected with 1x10$^{7}$ or 2${\times}$10$^{7}$ plaque forming units (pfu) of the recombinant AcNPV, oviposited number and egg weight were significantly decreased. Furthermore, the shape of the eggs was obviously divides into normal and abnormal shapes. The percentage of the eggs with an abnormal shape was 7.8% and 57.1% at 1${\times}$10$^{7}$ and 2${\times}$10$^{7}$ pfu inoculation, respectively. PCR analysis of the genomic DNA extracted from the eggs revealed that gfp and AcNPV ecdysteroid UDP-glucosyltransferase genes were amplified from both types of eggs with normal and abnormal shapes. The results demonstrate that AcNPV DNA, and gfp gene cloned into the AcNPV genome, injected in pupal stage were transmitted to eggs and remained stable through at least next generation.

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수중로봇용 AC구동시스템의 속도센서 제거를 위한 속도추정법 연구 (An Approach to a Speed Estimation Method to Remove Speed Sensor of Underwater Robot's AC Drive Systems)

  • 전봉환;임용곤;이판묵
    • 한국정보통신학회:학술대회논문집
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    • 한국해양정보통신학회 1998년도 춘계종합학술대회
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    • pp.371-376
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    • 1998
  • This paper describes an approach to a speed estimation method to remove speed sensor of underwater robot's AC drive systems. AC motors have been widely used in the field of underwater robot's manipulator or propulsion system. Most of these AC motors for underwater use have usually filled oil to compensate the high pressure in deep-sea operation, where a resolver is adopted to feed back the speed of rotor But this kind of speed feedback devices gives rise to some defects arising from their mechanical complexity and numerous signal lines; a resolver needs 6 or 7 signal lines for proper operation. This paper presents a speed estimation method to improve these problems of induction motor, which is adopted as a prototype of AC motor. The proposed speed estimation method is based on the RFO(rotor flux orientation) vector control method of voltage-fed AC drives. Using the controller of voltage-fed AC drives, it is unnecessary to measure the voltage for the estimation of rotor speed, which reduces the effects of measurement error Numerical simulation is carried out to investigate the validity of the method and the effects of rotors resistance variation.

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대형연소기에 적용되는 저주파 전자식 점화 트랜스 개발에 관한 연구 (A Study on the Development of Low Frequency Electronic Ignition Trans for Large Combustors)

  • 이호균;박정철
    • 한국정보전자통신기술학회논문지
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    • 제15권4호
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    • pp.223-229
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    • 2022
  • 본 논문은 보일러에 사용되는 점화트랜스에 대해 연구를 하였다. 점화봉 길이와 점화봉 간격 변화에 관계없이 출력주파수는 59.5~61.3Hz 사이에서 측정되었고 저주파 회로는 정상적으로 동작된 것을 알 수 있었다. 점화봉 간격이 2~10mm 변화를 주었을 때, 점화봉 길이가 30cm에서는 2.8A에서 3.45A까지 측정되었다. 점화봉 길이가 500cm에서는 9.37A에서 14.5A까지 측정되었고 1000cm에서는13.2A에서 32.6A까지 측정되었다. 점화봉 길이와 점화봉 간격이 증가 될수록 전류는 증가되었다. 2차코일 출력전압을 측정한 결과. 점화봉 길이가 30cm에서는 AC 0.84kV~AC 1.75kV로 측정되었고 500cm 인 경우는 AC 1.17kV~AC 1.944로 측정되었고 1000cm에서는 AC 1.4kV~AC 7.18kV까지 측정되었다. 점화봉 길이와 점화봉 간격이 증가되면 2차코일 출력전압도 증가되었다. 점화트랜스의 출력전압 측정한 결과, 점화봉 길이가 30cm에서는 DC 1.11kV~DC 1.57kV 까지 측정되었고 500cm 인 경우는 DC 2.49kV~DC 3.72kV, 1000cm에서는 DC 3.78kV~DC 9.42kV까지 측정되어서 점화봉 길이와 점화봉 간격이 증가되면 출력전압이 증가된 것을 알 수 가 있었다.

Effects of PNF Technique on Delayed Onset Muscle Soreness After Eccentric Exercise

  • Lee, Su-Young;Yi, Chung-Hwi;Choi, Mun-Suk
    • 한국전문물리치료학회지
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    • 제14권4호
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    • pp.1-6
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    • 2007
  • This study examined the effects of hold-relax with agonist contraction (HR-AC) on the symptoms of delayed onset muscle soreness (DOMS) induced by intensive eccentric exercise of the non-dominant biceps brachii. Ten men (mean age=26.7 yrs, mean height=172.1 cm, mean weight=66.2 kg) and ten women (mean age=27.4 yrs, mean height=165.9 cm, mean weight=60.7 kg) who had not participated in a regular exercise program for the upper extremities in the previous six months were randomly assigned to one of two experimental groups: the HR-AC group, or the control group. We measured joint range of motion (ROM), maximal voluntary isometric contraction (MVIC), and muscle soreness before eccentric exercise, and 24, 48, and 72 hours after eccentric exercise. The subjects in the HR-AC group received the HR-AC technique in the non-dominant biceps brachii. The HR-AC technique was applied 24 and 48 hours after eccentric exercise. There was no significant difference between the HR-AC and the control group. However, the HR-AC group, compared to the control group, had a significant difference between the time points of the various parameters. Increased ROM (p<.05), decreased muscle soreness (p<.05), and reduced MVIC (p<.05) were found in the HR-AC group after 72 hours. Decreased ROM (p<.05) and MVIC (p<.05), and increased muscle soreness (p<.05) were observed in the control group. These findings suggest that the HR-AC technique effectively reduces muscle soreness and increases ROM 72 hours after eccentric exercise.

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척추 뼈 SPECT/CT검사에서 경피적 척추성형술 시행 환자의 골 시멘트로 인한 인공물과 CT보정방법의 유용성 평가 (Usefulness Evaluation of Artifacts by Bone Cement of Percutaneous Vertebroplasty Performed Patients and CT Correction Method in Spine SPECT/CT Examinations)

  • 김지현;박훈희;이주영;남궁식;손현수;박상륜
    • 핵의학기술
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    • 제18권1호
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    • pp.49-61
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    • 2014
  • 인구의 고령화와 함께 골다공증성 척추압박골절 환자의 발병률은 매년 증가하는 추세이며 경피적 척추성형(percutaneous vertebroplasty, PVP)은 가장 많이 시행되는 표준화된 치료 방법이다. 시술 전 후의 정확한 진단평가에 있어 SPECT/CT 검사의 유용성이 우수하다는 연구 보고가 있으나 시술에 사용되는 골 시멘트(bone cement) 물질은 CT 영상에서 인공물(artifact)을 생성하여 영상품질에 영향을 미친다. 이에 본 연구에서는 골 시멘트가 SPECT/CT 영상에 미치는 영향을 평가하는 데 목적을 두었다. NEMA-1994 팬텀(phantom)에 배후방사능(3.6 kBq/ml), 열소(29.6 kBq/ml), 냉소(물)를 설정한 후 각 원통(cylinder)에 모형 시멘트를 삽입하여 영상 획득하였다. Astonish (Iterative: 4, Subset: 16)으로 재구성하였고, CT 보정 방법은 비감쇠보정(NAC), 감쇠보정(AC+SC-), 감쇠 및 산란보정(AC+SC+) 3가지 방법을 사용하였다. 각각의 보정방법에 따른 평균계수와 시멘트물질의 유 무에 따른 계수 변화율을 비교하고 대조도 회복 계수(contrast recovery coefficient, CRC)를 구했다. 또한, 압박골절 진단을 받은 환자 107명 중 PVP를 시행한 20명을 선별하여 추체를 골절, 정상, 시멘트로 나누고 연조직(척추기립근)을 포함 4곳의 평균 계수를 측정한 후, 뼈/연조직 간 계수 비(bone/soft tissue ratio, B/S ratio)을 구했다. NAC를 기준으로 AC+SC-, AC+SC+의 평균계수는 증가하였고, AC+SC-의 평균계수가 가장 높았으며, AC+SC+는 AC+SC-에 비해 상대적으로 낮았다. 시멘트 물질의 유 무에 따른 평균계수는 시멘트가 존재할 때 NAC, AC+SC-, AC+SC+에 따라 팬텀의 열소에서 12.4%, 6.5%, 1.5%, 냉소 75.2%, 85.4%, 102.9%, 배후방사능 13.6%, 18.2%, 9.1%, 임상영상의 골절부에서 33.1%, 41.4%, 63.5%, 정상부 53.1%, 61.6%, 67.7%, 연조직 10.0%, 4.7%, 3.6%의 증가율을 보였다. 반면 원통 내부의 시멘트 인접부에서 상대적 계수감소를 확인할 수 있었고, 병소에 대한 팬텀영상과 임상영상간의 계수 증가율은 상반된 양상을 보였다. 대조도 비를 나타내는 CRC와 B/S ratio는 NAC, AC+SC-, AC+SC+순으로 향상되었고, 팬텀의 냉소에서는 큰 변화 없이 일정하였다. 정량적 계수는 AC+SC-가, 대조도 비는 AC+SC+가 가장 높게 분석되었다. PVP 시행 환자의 척추 뼈 SPECT/CT 검사에서 AC+SC- 의 사용은 영상계수를 대폭 향상시켜 특히 병소의 농도가 높은 영상을 얻는 반면에 골 시멘트의 영향과 함께, 연부조직 및 산란영역의 영상잡음 계수까지 증가시키므로 대조도비를 향상시키는 AC+SC+의 적용을 병행한다면 임상진단에 유용할 것으로 사료된다.

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거대고리 아자크라운화합물과 전이금속 및 란탄족금속이온의 착물의 안정도 (The Stability Constant of Transition and Lanthanide Metal Ions Complexes with 15 Membered Macrocyclic Azacrown Ligands)

  • 홍춘표;최용규
    • 대한화학회지
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    • 제48권6호
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    • pp.577-582
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    • 2004
  • 새로운 아자크라운 화합물인, 1,4-dioxa-7,10,13-triazacyclopentadecane-N,N',N''-triacetic acid, $N-ac_3[15]aneN_3O_2(II_a)$과 1,4-dioxa-7,10,13-triazacyclopentadecane-N,N',N''-tripropioc acid, $N-pr_3$[15]ane$N_3O_2(II_b)$는 이와 유사한 화합물의 합성방법을 수정하여 합성하였다. 합성된 이 아자크라운 화합물, $N-ac_3[15]aneN_3O_2와\;N-pr_3[15]aneN_3O_2$의 양성자화 상수값은 전위차법을 이용하여 PKAS 프로그램으로부터 측정되어졌다. 그리고 0.1 M $NaClO_4$수용액으로 이온강도를 조절하고, $25{\pm}0.1^{\circ}C$에서 란탄족금속인, $Ce^{3+},\;Eu^{3+},\;Gd^{3+}$$Yb^{3+}$과 리간드인 $N-ac_3[15]$ane$N_3O_2$$N-pr_3[15]aneN_3O_2$의 착물의 안정도상수를 전위차법을 이용하여 BEST 프로그램으로부터 구하였고, 또한 위와 같은 조건에서 전이금속인 $Co^{2+}$, $Ni^{2+}$, $Cu^{2+}$$Zn^{2+}$와 리간드인, $N-ac_3[15]aneN_3O_2$$N-pr_3[15]aneN_3O_2$의 착물의 안정도상수 값도 전위차법을 이용하여 BEST 프로그램으로부터 측정하였다. 합성된 아자크라운 화합물과 골격구조가 유사하고 아세트산과 프로피온산기를 포함하는, 1,7-dioxa-4,10,13-triazacyclopentadecane-N,N',N''-triacetic acid과 1,7-dioxa-4,10,13-triazacyclopentadecane-N,N',N''-tripropioc acid의 착물의 안정도를 비교분석하였다.

Two-Switch Auxiliary Resonant DC Link Snubber-Assisted Three-Phase Soft Switching PWM Sinewave Power Conversion System with Minimized Commutation Power Losses

  • Nagai, Shinichiro;Sato, Shinji;Ahmed, Tarek;Nakaoka, Mutsuo
    • Journal of Power Electronics
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    • 제3권4호
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    • pp.249-258
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    • 2003
  • This paper presents a high-efficient and cost effective three-phase AC/DC-DC/AC power conversion system with a single two-switch type active Auxiliary Resonant DC Link (ARDCL) snubber circuit, which can minimize the total power dissipation. The active ARDCL snubber circuit is proposed in this paper and its unique features are described. Its operation principle in steady-state is discussed for the three phase AC/DC-DC/AC converter, which is composed of PWM rectifier as power factor correction (PFC) converter, sinewave PWM inverter. In the presented power converter system not only three-phase AC/DC PWM rectifier but also three-phase DC/AC inverter can achieve the stable ZVS commutation for all the power semiconductor devices. It is proved that the proposed three-phase AC/DC-DC/AC converter system is more effective and acceptable than the previous from the cost viewpoint and high efficient consideration. In addition, the proposed two-switch type active auxiliary ARDCL snubber circuit can reduce the peak value of the resonant inductor injection current in order to maximize total system actual efficiency by using the improved DSP based control scheme. Moreover the proposed active auxiliary two-switch ARDCL snubber circuit has the merit so that there is no need to use any sensing devices to detect the voltage and current in the ARDCL sunbber circuit for realizing soft-switching operation. This three-phase AC/DC-DC/AC converter system developed for UPS can achieve the 1.8% higher efficiency and 20dB lower conduction noise than those of the conventional three-phase hard-switching PWM AC/DC-DC/AC converter system. It is proved that actual efficiency of the proposed three-phase AC/DC-DC/AC converter system operating under a condition of soft switching is 88.7% under 10kw output power.

절연형 고효율 PFC AC-DC 컨버터에 관한 연구 (A Study on PFC AC-DC Converter of High Efficiency added in Electric Isolation)

  • 곽동걸;김상훈
    • 전기학회논문지
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    • 제58권7호
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    • pp.1349-1355
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    • 2009
  • This paper is studied on a novel power factor correction (PFC) AC-DC converter of high efficiency by soft switching technique. The input current waveform in the proposed converter is got to be a sinusoidal form composed of many a discontinuous pulse in proportion to the magnitude of a ac input voltage under the constant switching frequency. Therefore, the input power factor is nearly unity and the control method is simple. The proposed converter adding an electric isolation operates with a discontinuous current mode (DCM) of the reactor in order to obtain some merits of simpler control, such as fixed switching frequency, without synchronization control circuit used in continuous current mode (CCM). To achieve the soft switching (ZCS or ZVS) of control devices, the converter is constructed with a new loss-less snubber for a partial resonant circuit. It is that the switching losses are very low and the efficiency of the converter is high, Particularly, the stored energy in a loss-less snubber capacitor recovers into input side and increases input current from a resonant operation. The result is that the input power factor of the proposed converter is higher than that of a conventional PFC converter. This paper deals mainly with the circuit operations, theoretical, simulated and experimental results of the proposed PFC AC-DC converter in comparison with a conventional PFC AC-DC converter.