• Title/Summary/Keyword: AAT

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Identification of pol Gene Mutation among BLV Proviruses Found in the Southern Province of Korea

  • Kwon, Oh-Sik
    • The Journal of Korean Society of Virology
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    • v.30 no.2
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    • pp.131-139
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    • 2000
  • Bovine leukemia virus (BLV) is an etiological agent of chronic diseases in cows worldwide. The BLV is one of retroviruses that contain a multi-functional enzyme, reverse transcriptase produced from the pol gene in its genome. We have sequenced some regions in the pol gene of BLV proviruses found in the Southern province of Korea from samples that turned out to be BL V positives by a PCR analysis. On the 5' side of the BLV pol gene (polymerase region), it was found that there were four leucines located at every 7 amino acids. They can form a leucine zipper motif that was not same as the pol gene of Japanese BLV isolate. The sequencing result of the proviral pol gene in Korean-type BLV also revealed some mutations leading to amino acid changes such as $CCT(Pro){\to}CTC(Leu)$, $AAT(Asn){\to}AAA(Lys)$, and non-sensible variations i.e., $TCT(Ser){\to}TCC(Ser)$, $ATT(Ile){\to}ATC(I1e)$ and $ACG(Thr){\to}ACA(Thr)$. On the 3' side of the pol gene (integrase region), some nucleotide sequences were mutated and led to amino acid changes. Among them, a mutation, $GAA(Glu){\to}GAC(Asp)$ occurred in many Korean-type BLV proviruses was very interesting because the amino acid was regarded as one of the most conserved amino acids in the retroviral integrase. It was also notable that the mutation on any leucine residue did not occur, in spite of its frequent appearance.

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A Study about the Effect of Reading Enhancement and the Selfrespect Improvement of Children through an Animalassisted Therapy (동물매개치료를 통한 독서부진 아동의 자아존중감 및 독서증진 효과에 관한 연구)

  • Her, Sun-Yeong;Hong, Hyun-Jin
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.24 no.3
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    • pp.181-198
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    • 2013
  • This study takes aim at finding how effective the usage of 'Read to Dogs' is for self-respect and reading improvement of elementary school students. For this, this study was compared and analyzed with pretest and posttest using 'tree pictures' of HTP and 'family pictures with motion' of KFD through performing, surveying, and art-therapy treatments. The result of this study showed that reading program using a reading -assistance- dog has a good effect on self-respect of children with reading inactivity. Also, this result will be used as useful information in developing and operating programs for children with reading inactivity in libraries and schools.

ESTIMATED PRODUCTIVITY OF THE GIANT MAGELLAN TELESCOPE (거대 마젤란 망원경의 논문 생산성 예측)

  • Ahn, S.H.;Park, B.G.;Kim, Y.S.;Chun, M.Y.;Kim, H.I.;Sung, H.I.;Lee, D.W.;Kim, S.C.
    • Publications of The Korean Astronomical Society
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    • v.23 no.2
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    • pp.123-128
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    • 2008
  • Productivity of the Giant Magellan Telescope is estimated based on the annual number of papers produced by the currently operating large telescopes such as the telescope at the ESO La Silla observatory, CFHT, AAT, the Magellan telescopes, ESO VLT, Japanese Subaru, the Gemini telescopes, and the Keck telescopes. We find that the amount of papers produced by a large telescope is roughly proportional to the diameter of its primary mirror. With this fact, we estimate the SCI-paper productivity of the Giant Magellan Telescope by extrapolating the productivity of the above-mentioned large telescopes. Moreover, according to the paper written in 2001 by Benn and Sanchez, the amount of highly-cited papers produced by a large telescope is roughly proportional to the light-gathering power of the telescope or the square of the diameter. Hence, we survey the productivity of Nature-class papers of the large telescopes and extrapolate the relationship to estimate the productivity of the Nature-class papers by using the Giant Magellan telescope of a filled aperture 21.4 meters in diameter. We expect that Korean astronomers will be able to produce annually 60 SCI-class papers and 20 Nature-class papers with high scientific impact by using the telescope-time corresponding to the 10% share of the Giant Magellan Telescope.

The effects of an animal-assisted therapy (AAT) program on depression and self-esteem of adolescents as victims of school violence (동물매개치료 프로그램이 학교폭력 피해경험 청소년의 우울과 자아존중감에 미치는 영향)

  • Park, Hyoung-Joon;Kim, Chung-Hui
    • Korean Journal of Veterinary Service
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    • v.35 no.4
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    • pp.327-332
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    • 2012
  • This study aimed to see if as companion animals living with human beings, dogs could provide emotional stability or play a therapeutic role for human beings. Of 12 middle school boys as victims of school violence, 6 were assigned to the experimental group that was provided with an animal-assisted therapy program over a total of 12 sections, one section per week, 60 minutes per section, between September and November 2011, followed by post-test 1 and, one month later, post-test 2. In the experimental composition, the remaining 6 boys were assigned to the comparison group that was allowed to read freely. Analysis was performed by using the scales of depression and self-esteem, and the results showed that the experimental group provided with the animal-assisted therapy program generated more significant therapeutic effects than the comparison group. The therapeutic effects were found to persist one month after the completion of the program. Therefore, it is believed that sensitive adolescents who suffer from depression or have lost self-esteem because of school violence can significantly benefit from an animal-assisted therapy program by reducing the recovery time or by getting lots of help with emotional stability. Further research on the basis of this study is expected to help adolescents with emotional therapy in other areas.

The Effect of Green Oak Acorn (Quercus ilex) Based Diet on Growth Performance and Meat Fatty Acid Composition of Broilers

  • Bouderoua, Kaddour;Mourot, Jacques;Selselet-Attou, Ghalem
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.6
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    • pp.843-848
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    • 2009
  • This experiment was conducted to compare the effects of oak acorns and corn on broilers growth performance, carcass characteristics and meat fatty acid (FA) composition according to different ages of broilers. Two separate groups of 400 male broilers, reared for six weeks, were fed diets containing 33.5% green oak acorns (GO) and 67% of corn (C), respectively. At day 35, the body weight of the chickens fed the GO diet was 12% lower (p<0.05) compared with controls (C). However, at day 56 birds reached similar final weights. Abdominal adipose tissue (AAT) was 78% higher in the controls compared to those fed the oak acorn-based diet. The total lipids (TL) of thigh muscle were higher in the controls compared to dietary treatment GO (3.1 against 2.5 g/100 g of muscle). There were significant differences in fatty acid (FA) composition of the muscle between two groups. The broilers fed oak acorn exhibited more C18:2, as well as a higher proportion of polyunsaturated fatty acid (PUFA) and PUFA:SFA (saturated fatty acid) ratio than those on the control diet, but no significant differences were observed among groups for the age of birds. There was a higher proportion of linolenic acid in broilers fed the oak acorn compared to the controls (0.8 vs. 0.6%). Furthermore, linolenic acid content decreased with age (p<0.001) in both groups. The results indicated no significant differences in FA with the age of birds. In conclusion, the meat fatty acids provided by the GO diet appeared to improve the nutritional quality preferred by the consumer.

Effect of Beta-Asarone on Impairment of Spatial Working Memory and Apoptosis in the Hippocampus of Rats Exposed to Chronic Corticosterone Administration

  • Lee, Bombi;Sur, Bongjun;Cho, Seong-Guk;Yeom, Mijung;Shim, Insop;Lee, Hyejung;Hahm, Dae-Hyun
    • Biomolecules & Therapeutics
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    • v.23 no.6
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    • pp.571-581
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    • 2015
  • ${\beta}$-asarone (BAS) is an active component of Acori graminei rhizoma, a traditional medicine used clinically in treating dementia and chronic stress in Korea. However, the cognitive effects of BAS and its mechanism of action have remained elusive. The purpose of this study was to examine whether BAS improved spatial cognitive impairment induced in rats following chronic corticosterone (CORT) administration. CORT administration (40 mg/kg, i.p., 21 days) resulted in cognitive impairment in the avoidance conditioning test (AAT) and the Morris water maze (MWM) test that was reversed by BAS (200 mg/kg, i.p). Additionally, as assessed by immunohistochemistry and RT-PCR analysis, the administration of BAS significantly alleviated memory-associated decreases in the expression levels of brain-derived neurotrophic factor (BDNF) and cAMP-response element-binding protein (CREB) proteins and mRNAs in the hippocampus. Also, BAS administration significantly restored the expression of Bax and Bcl-2 mRNAs in the hippocampus. Thus, BAS may be an effective therapeutic for learning and memory disturbances, and its neuroprotective effect was mediated, in part, by normalizing the CORT response, resulting in regulation of BDNF and CREB functions and anti-apoptosis in rats.

Effect of Aminosiloxane Modifier on Chemorheological Properties of Ortho-cresol Novolac Epoxy (Ortho-cresol Novolac형 에폭시의 화학레올로지 특성에 미치는 아민 개질제의 영향)

  • 김윤진;안병길;김우년;서광석;김환건;윤초규
    • Polymer(Korea)
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    • v.26 no.1
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    • pp.88-97
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    • 2002
  • The effect of aminosiloxane modifier on the chemorheological properties of ortho-cresol novolac epoxy/phenol novelac/triphnylphosphine resin system was investigated aat different isothermal curing temperatures. By adding the aminosiloxane to the resin system, not only conversion rate and conversion were increased but also glass transition temperature was promoted. Critical conversion and gelation time obtained at the crossover point between storage and loss moduli were reduced and thus the viscosity was increased by the aminosiloxane. $C_1$ and $C_2$ in the WLF equation calculated from the glass transition temperature as a function of conversion and measured viscosity were found to vary with the curing temperature. By applying the change of glass transition temperature with conversion, $C_1$ and $C_2$ to WLF equation, it was possible to predict accurately the viscosity change with isothermal curing reaction.

Genetic Structure of Pinus rigida Mill. in an Expanding Population Originating from a Few Founder Trees (수본(數本)의 양친수(兩親樹)에 의해 전파증식(傳播増殖)중에 있는 리기다소나무 집단(集團)의 유전적(遺傳的) 구조(構造))

  • Chung, Min Sup
    • Journal of Korean Society of Forest Science
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    • v.72 no.1
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    • pp.16-26
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    • 1986
  • Allozyme study on a small pitch pine stand originating and expanded rather rapidly from a few founder trees indicated that the colonization of the pitch pine population was made progressively from the place where the founder trees located to another by moving in cohorts of seeds from a limited number of family or genetically closely related family groups in line with the succeeding generations. This pattern of migration and colonization resulted marked differences in allelic and genotypic frequencies at many of the allozyme loci between the initially colonized subpopulation on the south-facing slope and the lately colonized subpopulation on north-facing slope of a hill. It appeared that gene fixation due to inbreeding and genetic drift occurred at some loci in the pitch pine population or subpopulations. However, even in t 1e inbreeding small pitch pine population or subpopulations, a comparatively large amount of genetic diversity or heterozygosity was maintained due to the high levels of gene recombination at many of the gene loci and natural selections favoring for heterozygotes.

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THE PHOTOGRAPIC PHOTOMETRY OF THE GLOBULAR CLUSTER NGC 6752

  • Lee, Kang-Hwan;Lee, See-Woo;Jeon, Young-Beom
    • Journal of The Korean Astronomical Society
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    • v.28 no.2
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    • pp.153-167
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    • 1995
  • More than 22,300 stars in NGC 6752 were measured over the region of 5' < r < 23' in B and V AAT plates. The most of these are main sequence(MS) stars and about 130 blue horizontal branch(BHB) stars were detected. The C-M diagram of all measured stars shows gaps appearing at $V{\approx}15.^{m}2$ and $16.^{m}2$ along the red giant branch(RGB) and their appearance shown by Lee & Cannon(1980) is found to be independent of measured region. The bimodal distribution of BHB stars is confirmed again and a wide gap shown by Lee & Cannon(1980) at $V{\approx}16^m$ is clearly seen for stars in the outer part (8' < r < 13') in the cluster. It is noted, however, that this gap is occupied by about a dozen of BHB staIs located in the inner region (5' < r < 8'). The number ratio of bright BHB star (V < $15^m$) to faint BHB stars (V > $15^m$) decreases with increasing radial distance from the cluster center. Three faintest BHB stars were found, and two stars ($V{\approx}18.^{m}5$) of there are located in the inner region of $r{\approx}6'$ and the other faintest one ($V{\approx}19.^{m}3$) located in the outer part of $r{\approx}13'$. Also a bluest star of (B - V) $\approx$ -0.5 at $V{\approx}17.^{m}2$ is found but it is located at the outer part of $r{\approx}13'$ in NE region. Therefore, the membership of the faintest BHB star and bluest star is suspected. The luminosity function(LF) and mass function(MF) for NGC 6752 were derived for MS stars. The LF for stars of $M_v\;<\;6^m$ in the outer part of r > 8' shows a consistency with that derived by Penny & Dickens(1986).

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Evaluation of Mutation Rate by Differences of Isozyme Band Patterns on $M_2$ Seedling Treated with Chemical Mutagen in Barley (보리 화학돌연변이제 처리 $M_2$ 유묘의 Isozyme band pattern 차이에 의한 돌연변이율 검정)

  • Bon Cheol, Koo;M., Kucharska
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.2
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    • pp.214-219
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    • 1997
  • Mutation rate of M$_2$ plants that were treated with three types of double treatments of chemical mutagens(1.5mol Na$N_2$ + 0.75mol MNH, 0.75mol MNH + 0.75mol MNH and 0.5mol MNH + 0.5mol MNH) were estimated on the rate of chlorophyll mutant, changes of isozyme loci ; esterase (Est), glutamate oxaloacetate transaminase(GOT ; AAT) and leucyl aminopeptydase(LAP ; AMP). Rate of chlorophyll mutants (3.3% =no. of seedling carrying mutant / all number of M$_2$ seedlings $\times$ 100) and rate of esterase isozyme loci mutants(3.5% =no. of plant carrying mutant / all number of M$_2$ plant) in Dema were higher than one of Sacheon 6, but no significant differences in GOT, LAP. Among isozymes, most of mutants in M$_2$ plant of two varieties were found in esterase (73% of total mutants were occurred in esterase loci). Although many of null bands were found in GOT 3, these were not repeatable and no real mutants. It might be due to qualities of starch, amount of extract buffer and degradation of isozyme during electrophoresis and staining.

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