• Journal of the East Asian Society of Dietary Life
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• v.12 no.1
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• pp.15-22
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• 2002

This study was carried out to investigate antimutagenic and cytotoxic effects of Korean traditional Mackjang added with sea tangle powder. The content percentage of most of minerals among general composition increased by adding sea tangle powder. By the Ames test, the antimutagenic effect of ethanol extract of Korean traditional Mackjang added with 5% sea tangle powder was strongest exceeding the control, 10%, and 15% sea tangle additions. The ethanol extract (400$\mu$g/plate) of Mackjang added with 5% sea tang1e powder in the S. typhimurium TA 100 strain showed inhibition rate of 95.0% against the mutagenesis induced by MNNG. The inhibition rate of ethanol extract (400$\mu$g/plate) of Mackjang added with 5% sea tang1e powder in the S. typhimurium TA98 and TA100 strains was 81.4% and 88.8%, respectively, against the mutagenesis induced by 4NQO. Under the same conditions, the suppression against B($\alpha$)P and Trp-P-1 in the TA98 and TA100 strains were 85.3% and 91.0%, and 96.5% and 96.5%, respectively. For the anticancer effects, an investigation was done on the cytotoxicity of Mackjang added with 5% sea tangle powder on the cell lines with human lung carcinoma (A549), human hepatocellular carcinoma (HepG2), and human gastric carcinoma (KATOIII). The cytotoxicities were inhibited with increasing the extract concentration. The treatment of 1.0 mg/mL Mackjang added with 5% sea tang1e powder showed relatively strong cytotoxicity of 61.2%, 61.8% and 66.8% against A549, KATOIII, and HepG2, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.2
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• pp.322-328
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• 2002

This study was carried out to investigate antimutagenic and anticancer effects of ethanolic extract of Korean traditional doenjang added sea tangle. Most of the mineral content of doeniang was increased by addition of sea tangle. In the Ames test, the antimutagenic effect of ethanol extract of Korean traditional doenjang added 5% sea tangle was higher than that of control (no additive), 10%, and 15% sea tangle additions. The inhibition rate of ethanol extract (200$\mu\textrm{g}$/plate) of doenjang added 5% sea tangle in the S. typhimurium TA100 strain showed 97.0% inhibition against the mutagenesis induced by MNNG. In addition, the suppression of ethanol extract (200$\mu\textrm{g}$/plate) of doenjang added 5% sea tangle in the S. typhimurium TA98 and TA100 strains showed 60.2% and 69.1% inhibition respectively, against the mutagenesis induced by 4NQO. The suppressions under the same condition against B($\alpha$ )P and Trp-P-1 in the TA98 and TA100 strains were 71.7% and 87.3%, and 66.6% and 80.8%, respectively. In the anticancer effects, the cytotoxicity of doenjang added 5% sea tangle on the cell lines with human lung carcinoma (A549), human hepatocellular carcinoma (HepG2), and human gastric carcinoma (KATOIII) were inhibited with increasing the extract concentration. The treatment of 1.0 mg/mL Doenjang added 5% sea tangle showed strong cytotoxicity of 56.4%, 87.67%, and 89.5% against A549, HepG2, and KATOIII, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.2
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• pp.211-216
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• 2003

This study was carried out to determine the antioxidative, antimutagenic, and anticancer effects of Rhodiola sachalinensis root using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical donating method, Ames test and cytotoxicity, respectively. Rhodiola sachalinenis root were extracted with ethanol and then further fractionated to n-hexane, chloroform, ethyl acetate (EtOAc), butanol and water, stepwise. Among five fractions, the Etohc fractions showed the highest electron donating activities (14.3 $\mu$g/mL). The inhibition rate of ethanol extract (200$\mu$g/plate) of Rhodiola sachalinensis root in the S. typhimurium TA100 strain showed 89.1% inhibition against the mutagenesis induced by MNNG. In addition, the suppression of EtOAc fractions with same concentration of Rhodiola sachalinensis root in the S. typhimurium TA98 and TAI00 strains showed 89.7% and 91.5% inhibition against 4NQO, respectively. The suppressions under the same condition against B($\alpha$)P and Trp-P-1 in the TA98 and TA100 strains were 94.2% and 95.7%, and 92.3% and 93.8%, respectively. The cytotoxic effects of Rhodiola sachalinensis root against the cell lines with human lung carcinoma (A549), human hepatocellular carcinoma (HepG2), human gastric carcinoma (AGS) and human breast adenocarcinoma (MCF-7) were inhibited with the increase of the extract concentration. The treatment of 1.0 mg/mL Rhodiola sachalinensis root of EtOAc fraction showed strong cytotoxicities of 90.5%, 81.5%, 92.2% and 82.6% against A549, HepG2, AGS and MCF-7, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.4
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• pp.410-415
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• 2008

This study was performed to observe the antimutagenic and cytotoxic activities of methanol extract of kochujang added with sea tangle and deep sea water salts (SDK) and kochujang added with sea tangle (SK) using the Ames test and SRB assay, respectively. The direct antimutagenic effect of SDK and SK methanol extracts were examined by Ames test using Salmonella Typhimurium TA98 and TA100. In the Ames test, methanol extract of SDK and SK alone did not exhibit mutagenicity and most of the samples showed high antimutagenic effects against mutation induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and 4-nitroquinoline-1-oxide (4NQO). Methanol extract of SDK ($200{\mu}g$/plate) showed approximately 71.4% inhibitory effect on the mutagenesis induced by 4NQO against TA98 strain; whereas 56.1% and 83.6% inhibitions were observed on the mutagenensis induced by 4NQO and MNNG against TA100 strain. The cytotoxic effects of SDK and SK increased with increasing sample concentration against human cervical adenocarcinoma (HeLa), human hepatocellular carcinoma (Hep3B), human breast adenocarcinoma (MCF-7), human stomach adenocarcinoma (AGS), and human lung carcinoma (A549). The SDK at the concentration of 1 mg/ml showed cytotoxicities of 61.5%, 61.3%, 51.4%, 57.9% and 77.7% against HeLa, Hep3B, MCF-7, AGS and A549, respectively. In contrast 1 mg/ml treatment of SDK and SK methanol extract had only $2{\sim}38%$ cytotoxicity on human transformed primary embryonal kidney cell (293).

• Archives of Pharmacal Research
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• v.20 no.5
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• pp.491-495
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• 1997

All fifteen flavonoids (1-15) have been isolated from the roots of Sophora flavescens (Leguminosae) as active principles of the cytotoxic property toward human tumor cell lines such as A549, SK-OV-3, SK-Mel-2, XF498 and HCT15, in vitro. By means of spectral analyses, particularlyby the aid of various two dimensional NMR experiments, all $^1H-NMR$ ad $^{13}C$ -NMR signals of 1-15 were completely assigned, and thus the structures of 1-15 were established unambiguously.

• YAKHAK HOEJI
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• v.42 no.3
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• pp.233-237
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• 1998

Activity-guided fractionation and repeated column chromatography afforded two cytotoxic compounds R-3 and R-4 from the root of Rumex japonicus HOUTT. Compou nds were identified as musizin and emodin, respectively, by the physicochemical and spectral data. Besides R-3 and R-4, two compounds R-1 and R-2, chrysophanol and physcion, respectively, were also isolated. The compound R-3 and R-4 exhibited cytotoxicity against cultured human tumor cell lines, A-549, SK-OV-3, SK-MEL-2, XF498 and HCT15 with $ED_{50}$ values ranging from 2.68 to $10.06{\mu}g/ml$.

• Natural Product Sciences
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• v.1 no.1
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• pp.43-49
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• 1995

Two cucurbitane-compounds were isolated from the roots of Bryonia alba L. and the chemical structures were established as 19-norlanost-5-ene-3,1l,22-trione-$2{\beta}$, $16{\alpha}$,$20{\beta}$,25-tetrahydroxy-9-methyl (23,24-dihydrocucurbitacin D) and 2-O-${\alpha}$-D-glucopyranosyl 19-norlanost-5-ene-3,11,22-trione-$2{\beta}$,$16{\alpha}$,$20{\beta}$,25-tetrahydroxy-9-methyl (arvenin IV), respectively, on the basis of chemical and spectral methods. Both of the compounds showed cytotoxic activity against cancer cell lines, A549, SK-MEL-2, COLO 205 and L1210.

• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2002.11a
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• pp.133-133
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• 2002

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.212-212
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• 1994

본 연구소에서 분리하여 항암활성을 측정한바 있는 Sesquiterpene lactone계열의 KR-53170이 in vitro에 비해 in vivo에서 상대적으로 낮은 효력을 보이는데 착안 그 유도체들을 합성함으로써 in vivo stability 및 efficacy를 개선하고자 하였다. 합성된 물질의 in vivo activity는 human tumor cell중에서 A549(폐암), SK-OV-3(난소암1, SK-MEL-2(피부암), XF-498(중추신경계암), HCT-15(직장암)의 5개 암세포를 이용하여 ED$_{50}$($\mu\textrm{g}$/ml )를 측정하였으며 대조물질로는 KR-53170과 Cisplatin이 사용되었다. 그 결과 HR-53170의 terminal methyl ketone이 carboxylic acid로 변환된 CW-251001과 이것의 methyl ester인 CW-251002, ethyl ester인 CW-251003, 그리고 morpheline과 수용성으로 N-methylpiperazine, N-methyl-N'-aminopiperazine, 4-Piperidinopiperidine을 결합시켜서 합성한 CW-251011, CW-251012, CW-251013, CW-251014가 in vitro에서 어느 정도 활성을 유지하였다. 특히 이들중 CW-251001, CW-251012, CW-251013, CW-2s1014는 물에 대한 용해도가 상당히 개선되어 in vivo 활성검색을 위한 후보물질로 고려되었다. 하지만 이들중 CW-251001은 in vivo에서 낮은 농도에서는 활성이 거의 없었으며 놓은 농도에서는 독성을 나타내었다.

• Natural Product Sciences
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• v.8 no.3
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• pp.103-107
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• 2002

Four known sesquiterpenes, laurinterol (1), isolaurinterol (2), aplysinal (3) and aplysin (4) were isolated from the Korean red alga Laurencia okamurae off Cheju Island, Korea. Their structures were identified by comparison with the literature data. Compounds 1-4 showed potent cytotoxicity against A549, SK-OV-3, SKMEL-2, XF498, and HTl5 cell lines with $EC_{50}$ values ranging from 1.2 to 17.6 ${\mu}g/ml$.