• Journal of Convergence for Information Technology
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• v.12 no.4
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• pp.95-102
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• 2022

The purpose of this study was to compare the psychological state of nursing students according to classes in the face-to-face and non-face-to-face situations during COVID-19. Subjects were randomly expressed. As for the analysis method, the difference in the scores of the dependent variables between the two groups was analyzed by an independent t-test. As a result of the study, The degree of depression and anxiety between the two groups was higher in face-to-face students(8.16±9.48, 8.20±9.93) and non-face-to-face students(7.08±8.38, 6.53±8.23) but not statistically significant (t=1.143(.254), (t=1.728(.085)). These results are used for the basis for preparing a program for psychological relief for students participating in face-to-face classes, and a repeated study on psychological depression and anxiety among students is suggested in the future when social distancing is prolonged.

• Korean Journal of Microbiology
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• v.34 no.3
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• pp.115-119
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• 1998

We cloned the 4.8 kb BglII fragment containing genes downstream pHENX7 from Pseudomonas sp. strain DJ77. The restriction map of the resultant clone, recombinant plasmid pYCS500, was determined. Sequencing analysis of the 465 bp HindIII-ClaI fragment revealed an open reading frame of 282 bp that was then designated phnM. The deduced polypeptide is 93 amino acid residues long with a $M_r$ of 10,008. The PhnM has 37.3-53.9% identity with plant-type ferredoxin proteins such as NahT, XylT, DmpQ, AtdS, PhlG, PhhQ and TbuW and contains the motif similar to well-conserved functional domains of those proteins.

• Transactions of the Korean Society of Mechanical Engineers
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• v.12 no.1
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• pp.53-63
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• 1988

Using COD-R curves, instability of crack growth in ductile material is studied here. Tearing modulus $T_{\delta}$, based on the COA concept is defined and the instability criterion $T_{{\delta}{\app}}$> $T_{{\delta}{\mat}}$ is compared with experiments. Variations of the COD-R curve and the location of rotation center are experimentally investigated as the initial crack ratio varies. Three-point bend specimens of alloy steel SCM4 are used here and two clip-on gauges are installed in order to obtain the COD-R curve. The results show that the tearing modulus $T_{\delta}$ is a good measure to characterize the instability of crack growth. Also it is found that the location of rotation center is almost constant regardless of the initial crack ratio and the amount of the crack growth.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.8
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• pp.4059-4062
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• 2016

Frequencies of polymorphisms of genes BRCA1 and ТР53 in breast cancer (BC) patients with a BC family history and radiation history were assessed and compared in the Semey region of Kazakhstan. The study included 60 women directly irradiated by the activities of the Semipalatinsk test site with a calculated effective equivalent dose of 500 mSv and their first generation descendants (group BC+Her+Exp); 65 women with family BC and absence of radiological history - the effective equivalent dose due to anthropogenic sources not exceeding 50 mSv (group BC+Her-Exp). The comparison group consisted of 65 women patients with breast cancer without family and radiological history (BC-Her-Exp). The control group comprised 60 women without breast cancer and without family and radiological history (nonBC). We carried out the genotyping of the polymorphisms c.2311T>C, c.4308T>C and 5382insC of the BRCA1 gene and rs1042522 of the ТР53 gene. The frequency of the polymorphism c.2311T>C was significantly higher in patients of the group BC+Her+Exp than in healthy women, and of the polymorphism 5382insC in BC+Her+Exp compared to all other groups. The frequency of the rs1042522 polymorphism of ТР53 was significantly higher in all groups of patients with breast cancer compared with the control group. Differences between groups of women with breast cancer were significant only in BC+Her+Exp vs. BC+Her-Exp. Combinations of polymorphisms of the genes BRCA1 and TP53 predominated in women with a family and radiological history.

• Radiation Oncology Journal
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• v.17 no.1
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• pp.1-8
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• 1999

Purpose : Experimental studies have implicated the wild type p53 In cellular response to radiation. Whether altered p53 function can lead to changes in clinical radiocurability remains an area of ongoing study. This study was performed to investigate whether any correlation between change of p53 and outcome of curative radiation therapy in patients with head and neck cancels. Methods : Immunohistochemical analysis with a mouse monoclonal antibody (DO-7) specific for human p53 was used to detect to overexpression of protein in formalin fixed, paraffin-embedded tumor sample from 55 head and neck cancer patients treated with curative radiation therapy (median dose of 7020 cGy) from February 1988 to March 1996 at 51. Mary's Hospital. Overexpression of p53 was correlated with locoregional control and survival using Kaplan-Meier method. A Cox regression multi-variate analysis was peformed that included all clinical variables and status of p53 expression. Results : Thirty-seven (67.2$\%$) patients showed overexpression of p53 by immunohistochemical staining in their tumor. One hundred percent of oral cavity, 70$\%$ of laryngeal, 66.7$\%$ of oropharyngeal, 66.7$\%$ of hypopharyngeal cancer showed p53 overexpression (P=0.05). The status of p53 had significant relationship with stage of disease (P=0.03) and history of smoking (P=0.001). The overexpression of p53 was not predictive of response rate to radiation therapy. The locoregional control was not significantly affected by p53 status. Overexpression of p53 didn't have any prognostic implication for disease free survival and overall survival. Primary site and stage of disease were significant prognostic factors for survival. Conclusions : The p53 overexpression as detected by immunohistochemical staining had significant correlation with stage, primary site of disease and smoking habit of patients. The p53 overexpression didn't have any predictive value for outcome of curative radiation therapy in a group of head and neck cancers.

• The Journal of Korean Society of Virology
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• v.29 no.4
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• pp.235-245
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• 1999

HIV-1 Tat, a strong transactivator, is essential for the HIV-1 replication and AIDS progression. The Tat function is markedly inhibited by human p53 anti-oncogene. However, the detail mechanism has not yet been clearly revealed. In our previous report, we have addressed that p53 is unlikely to interact directly with HIV-1 Tat. In the consecutive experiments, Tat-phosphorylation was found to increase in proportional to the amounts of transfected p53. This work was initiated to identify the signaling factor that is involved in the p53-mediated Tat suppression. Several protein kinases were tested for the phosphorylation of Tat, and we found that PKR is likely to be involved in the p53-mediated Tat suppression. PKR was co-immunoprecipitated by anti-Tat antibody in the Tat-expressing Jurkat cell lysates only when the cells were transfected by p53, indicating that PKR-Tat interaction depends on the p53 activity. The interaction seems to result in PKR-mediated Tat-phosphorylation. Tat function was not blocked by p53 when co-transfected trasiently with antisense-PKR. We have generated PKR-knock out Jurkat cell clone. The PKR defective Jurkat cells didn't show the p53-mediated Tat suppression. These data indicate that p53-mediated Tat suppression is strongly associated with PKR. PKR-mediated Tat phosphorylation experiments are now under investigation by kinase assay and co-immunoprecipitation in the presence or absence of p53.

• Parasites, Hosts and Diseases
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• v.53 no.4
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• pp.493-496
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• 2015

Toxoplasmosis, caused by Toxoplasma gondii, is a parasitic zoonosis with worldwide distribution. The present study investigated the prevalence of T. gondii in dogs in Zhanjiang city, southern China, using both serological and molecular detection. A total of 364 serum samples and 432 liver tissue samples were collected from the slaughter house between December 2012 and January 2013 and were examined for T. gondii IgG antibody by ELISA and T. gondii DNA by semi-nested PCR based on B1 gene, respectively. The overall seroprevalence of T. gondii IgG antibody was 51.9%, and T. gondii DNA was detected in 37 of 432 (8.6%) liver tissue samples. These positive DNA samples were analyzed by PCR-RFLP at 3'- and 5'-SAG2. Only 8 samples gave the PCR-RFLP data, and they were all classified as type I, which may suggest that the T. gondii isolates from dogs in Zhanjiang city may represent type I or type I variant. This study revealed the high prevalence of T. gondii infection in dogs in Zhanjiang city, southern China. Integrated measures should be taken to prevent and control toxoplasmosis in dogs in this area for public health concern.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2001.04b
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• pp.69-70
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• 2001

포도주 부산물이 퇴비와 혼합처리 시용에 따른 고추생육에 미치는 영향을 구명하기 위해 평창군 비가림재배 시설하우스 농가에서 수행하였다. 초기 생육기인 정식 후 15에는 고추의 초장은 유의차가 인정되지 않았으나 정식 후 기간이 경과함에 따라 높은 증가추세를 나타내었으며, 경경의 경우 정식 후 45일, 75일에는 유의차(p>0.05)가 인정되었다. 대조구(돈분발효퇴비 2,000kg/10a)가 0.53cm에서 1.67cm로 1.14cm 증가하였는데, 처리구 T4(포도주부산물 400kg＋돈분발효퇴비 1,000kg/10a)는 0.5cm에서 1.74cm로 1.24cm 증가하여 대조구에 비하여 8.7% 높은 증가율을 보였다. 각 처리구의 엽록소 함량은 45일에 증가하다가 75일에 다시 감소하는 현상을 나타내었으며, 처리 15일에서 T1(포도주부산물 200kg＋돈분발효퇴비 2,000kg/10a)과 대조구 사이에 유의차가 인정되었으며, T1(포도주부산물 200kg＋돈분발효퇴비 2,000kg/10a)이 대조구(돈분발효퇴비 2,000kg/10a)보다 2.6이 높은 56.1로서 가장 높았으며, T4(포도주부산물 400kg＋돈분발효퇴비 1,000kg/10a)에서는 53.4로 대조구와 같은 수준을 나타내었다. 엽면적은 정식 후 45일에서 T4(포도주부산물 400kg＋돈분발효퇴비 1,000kg/10a)가 60.0$\textrm{cm}^2$로 가장 높게 나타났다. 홍고추의 주당 수량은 돈분발효퇴비와 포도주부산물을 같이 시비한 처리구가 대조구(돈분발효퇴비 2,000kg/10a)에 비하여 감소현상을 보였는데 이러한 현상은 생육후반기에 암모니아 가스에 의해 작물의 피해가 발생하여 수량이 감소된 것으로 사료된다.

• Parasites, Hosts and Diseases
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• v.53 no.6
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• pp.749-753
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• 2015

Toxoplasma gondii atypical type II genotype was diagnosed in a pet peach-faced lovebird (Agapornis roseicollis) based on histopathology, immunohistochemistry, and multilocus DNA typing. The bird presented with severe neurological signs, and hematology was suggestive of chronic granulomatous disease. Gross post-mortem examination revealed cerebral hemorrhage, splenomegaly, hepatitis, and thickening of the right ventricular free wall. Histologic sections of the most significant lesions in the brain revealed intralesional protozoan organisms associated with malacia, spongiform changes, and a mild histiocytic response, indicative of diffuse, non-suppurative encephalitis. Immunohistochemistry confirmed the causative organisms to be T. gondii. DNA isolated from the brain was used to confirm the presence of T. gondii DNA. Multilocus genotyping based on SAG1, altSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico markers demonstrated the presence of ToxoDB PCR-RFLP genotype #3 and B1 gene as atypical T. gondii type II. The atypical type II strain has been previously documented in Australian wildlife, indicating an environmental transmission route.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.5
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• pp.2313-2318
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• 2014

Peroxisome proliferator-activated receptor gamma (PPAR-${\gamma}$), a ligand-dependent nuclear transcription factor, has been found to widely exist in tumor tissues and plays an important role in affecting tumor cell growth. In this study, we investigated the effect of PPAR-${\gamma}$ on aspects of the cervical cancer malignant phenotype, such as cell proliferation and apoptosis. Cell growth assay, Western blotting, Annexin V and flow cytometry analysis consistently showed that treatment with troglitazone (TGZ, a PPAR-${\gamma}$ agonist) led to dose-dependent inhibition of cervical cancer cell growth through apoptosis, whereas T0070907 (another PPAR-${\gamma}$ antagonist) had no effect on Hela cell proliferation and apoptosis. Furthermore, we also detected the protein expression of p53, p21 and Mdm2 to explain the underlying mechanism of PPAR-${\gamma}$ on cellular apoptosis. Our work, finally, demonstrated the existence of the TGZ-PPAR-${\gamma}$-p53 signaling pathway to be a critical regulator of cell apoptosis. These results suggested that PPAR-${\gamma}$ may be a potential therapeutic target for cervical cancer.