• Title/Summary/Keyword: A30P

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Isolation of a Bacterium with Potent Antibacterial Activity against Swine Atrophic Rhinitis Bacteria and Antibacterial Activities (돼지 위축성 비염균에 대한 항균성을 나타내는 세균의 분리 및 항균활성)

  • 이종수;강석휘;서승보;김재호
    • KSBB Journal
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    • v.17 no.3
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    • pp.317-320
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    • 2002
  • A bacterium(Y-5) which has potent antibacterial activity against swine atrophic rhinitis bacteria (Bordetella bronchiseptica, Pasteurella multocida) was isolated from soil and identified as a strain of Bacillus sp. Y-5 upon investigation of the morphological and physiological characteristics. The culture broth obtained from incubation of the Y-5 strain at $30^{\circ}C$ for 21 h in tryptose-bouillon agar medium (pH 6.0) showed active antibacterial activity against Bordetella bronchiseptica and the culture broth that of $30^{\circ}C$, pH 6.5, 18 h showed active antibacterial activity against Pasteurella multocida.

Influence of Knowledge and Health Beliefs on Health Promoting Behavior about Osteoporosis in Working Women in their 20s and 30s (20~30대 직장여성의 골다공증 지식과 건강신념이 골다공증 예방 건강증진행위에 미치는 영향)

  • Kim, Min-Ju;Jo, Myoung-Ju
    • Journal of muscle and joint health
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    • v.30 no.2
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    • pp.138-146
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    • 2023
  • Purpose: The purpose of this study was to examine knowledge, health beliefs and health promoting behavior about osteoporosis in working women in their 20s and 30s. Methods: A survey was conducted among working women in their 20s and 30s who visited the health promotion center of B Medical Center in B Metropolitan City. The data were collected from December 2021 to March 2022 using structured self-reported questionnaires. SPSS/WIN 25.0 program was used to analyze the data. Results: Factors influencing health promoting behavior about osteoporosis in women in their 20s and 30s were in the order of "good" subjective health status (β=.47, p<.001), "moderate" subjective health status (β=.36, p<.001) and knowledge (β=.18, p=.015). These factors explained 12.4% of health promoting behaviors about osteoporosis. Conclusion: The results indicate the need to develop and implement healthcare programs that can improve the health status and provide knowledge to improve health promoting behavior about osteoporosis in women in their 20s and 30s.

The study on the separation characteristics of heavy metal ion by inorganic oxides and ion exchange resin (무기산화물 및 이온교환수지에 의한중금속 이온 분리특성 연구)

  • Dan, Cheol Ho;Kim, eong Ho;Yang, Hyun Soo
    • Clean Technology
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    • v.12 no.1
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    • pp.37-44
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    • 2006
  • The effectiveness of inorganic oxides (DT-30), anionic exchange resin (DT-60) and carbon absorbent (DT-80, DT-90) on the equilibrium and continuous separation characteristics and removal of cobalt, cesium and iodide ion in the waste water was investigated. As a result, DT-30, DT-80 or DT-90, and DT-60 showed excellent separation properties on the cesium, cobalt and iodide respectively. In the equilibrium experiment, the adsorption amount of cesium for DT-30 increased with temperature, but increasd largely with pH. In case of DT-80, adsorption of cobalt was depended on pH but was not influenced by temperature. In the continuous system by passing a heavy metal ion solution through the ion exchange tower, DT-30, DT-90 and DT-60 showed good separation characteristic for cesium, cobalt and iodide respectively. In this case, separation characterization of DT-30 on the cesium and of DT-60 on the iodide were better than that of DT-90 on the cobalt. From the experiment on the effect of impurities on the ion exchange characteristics, impurities such as surfactant and oil did not influence the efficiency of DT-90. In the mean while, ion separation capacity of DT-30 were decreased largely by impurities such as surfactant and oil. Also, surfactant had a strong influence on the effectiveness of DT-60. Accordingly, it turned out to be very important thing that impurities should be removed in the preprocessing stage.

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Effects of a Sodium Alginate Coating on Egg Quality during Storage (Sodium Alginate 코팅이 계란의 저장시 품질에 미치는 영향)

  • Hong, Wan-Pyo;Jeong, Yoon-Hwa;Ahn, Yong-Hyun
    • Journal of the East Asian Society of Dietary Life
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    • v.17 no.6
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    • pp.822-826
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    • 2007
  • This study was conducted to determine the effects of a 0.5% sodium alginate eggshell coating, that was applied at two temperatures, 5 and $25^{\circ}C$, by examining the degree of microbial inhibition on the eggshell surface as well as the coating's effects on egg quality during storage. A significant portion of human Salmonella enteritidis infection is traced to food contamination, and eggs are often highly exposed to this type of contamination. The 0.5% sodium alginate coating's effect for inhibiting microbial growth on the eggs, at $25^{\circ}C$ for 7 days, was one hundred thousand times more effective than that of the uncoated eggs. The pH level in eggs increases as the eggs lose $CO_2$ and as the storage temperature increases. We found that the pH of the coated eggs was lower than that of the uncoated eggs. The pH for the uncoated eggs changed from 7.72 to 7.94 over 30 days of storage at $5^{\circ}C$. However, when the eggs were coated with 0.5% sodium alginate, the pH changed from 7.72 to 7.85 over 30 days of storage at $5^{\circ}C$. The Haugh unit was 66.02 for the uncoated eggs and 70.37 for the 0.5% sodium alginate coated eggs after 30 days of storage. The yolk index of the eggs coated with sodium alginate was higher than that of the uncoated eggs after 30 days. These results indicate that a sodium alginate coating on eggs can serve as protection from microbes and is effective in preserving the interior quality of eggs.

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The Effects of Social support, Self-efficacy and Perceived health status on Aging anxiety of the Middle-aged women (중년여성의 사회적지지, 자기효능감, 지각된 건강상태가 노화불안에 미치는 영향)

  • Jung, Young-Ok;Oh, Hyo-Sook
    • Journal of Korean Public Health Nursing
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    • v.30 no.1
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    • pp.30-41
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    • 2016
  • Purpose: This study was conducted to determine the effects of middle-aged women's social support, self-efficacy, and perceived health status on aging anxiety. Methods: Research subjects were 210 middle-aged women between 40 - 60 years living in G-city. Data were collected from July $21^{st}$ 2014 to August $8^{th}$ using a structured questionnaire and analyzed using t-test, One way ANOVA, and multiple regression analysis using the SPSS/WIN 21.0 program. Results: Aging anxiety showed statistically significant differences in religion (t=2.44, p=.015), satisfaction with married life (F=4.39, p=.014), type of leisure activity (F=8.09, p<.001), and number of friends (F=3.23, p=.024). Aging anxiety showed correlation with social support (r=-.21, p=.003), self-efficacy (r=-.46, p<.001), and perceived health status (r=-.33, p<.001). Factors affecting aging anxiety were self-efficacy (${\beta}=-.33$, p<.001), perceived health status (${\beta}=-.23$, p<.001), leisure activity type (${\beta}=-.16$, p=.010), and religious affiliation (${\beta}=-.17$, p=.038). The predictive power of these variables was 25% (F=18.63, p<.001). Conclusion: Nursing intervention strategies for improving self-efficacy, perceived health status, and leisure activity are required to relieve aging anxiety of middle-aged women.

Comparative Antitumor Activity of Water Extracts from Fruiting Body of Phellinus linteus, Phellinus baumii and Phellinus gilvus (Phellinus linteus, Phellinus baumii 및 Phellinus gilvus 자실체 추출물의 항암효과 비교)

  • 배재성;황미현;장광호;이만휘;이근우;조우식;최성국;윤효인;임종환
    • Toxicological Research
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    • v.20 no.1
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    • pp.37-42
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    • 2004
  • This study was undertaken to investigate comparative anti-tumor activity of water extracts of Phellinus gilvus (PGE), Phellinus linteus (PLE), and Phellinus baumii (PBE) in vitro. The anti-tumor activity in the present study was evaluated by sulforhodamine B (SRB) and microtetrazolium (MTT) assay in terms of cell survival level. The tumor cells (sarcoma 180 and P388) were treated with PGE, PLE, and PBE (7.5, 15, and 30 $\mu\textrm{g}$/ml) and Doxorubicin (DOX) (0.001~10 $\mu\textrm{M}$). The results showed that DOX, PGE, and PLE inhibited proliferation showing a dose-dependent manner against both tumor cells. However, PBE was inhibited by the only 30 $\mu\textrm{g}$/ml in both cells proliferation. In conclusion, all of PGE, PLE, and PBE used in this study have shown anti-tumor activity against both sarcoma 180 and P388. Among them, PLE was the most effective in anti-tumor activity against sarcoma 180 (p<0.05) and PGE was against P388 in SRB assay. PLE, however, was against P388 (p<0.05) in MTT assay.

Effects of Feeding High- and Low- Forage Diets Containing Different Forage Sources on Rumen Fermentation Characteristics and Blood Parameters in Non-Pregnant Dry Holstein Cows

  • Peng, Jing Lun;Kim, Byong Wan;Lee, Bae Hun;Nejad, Jalil Ghassemi;Sung, Kyung Il
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.1
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    • pp.1-9
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    • 2017
  • This research was conducted to investigate the effects of feeding high and low forage diets with different forage sources on rumen fermentation characteristics and blood parameters of Holstein cows during the dry period. Eight Holstein cows were completely randomized assigned to two groups and repeated measurement was utilized in the analysis. Cows in two treatments were fed with diets with high (F:C = 70:30, 70F; forage source: mixed-sowing whole crop barley and Italian ryegrass silage, BIRG) and low (F:C = 55:45, 55F; forage source: tall fescue hay, TF) forage level. Rumen fluid pH was higher in 70F group. Levels of acetic acid, propionic acid, and butyric acid showed a similar pattern: from the lowest value at 07:30 h to the highest at 10:30 h and then decreased in both groups. The ratio of acetic acid to propionic acid was significantly higher (p < 0.05) in 55F group at 09:30 and 10:30 h. Rumen fluid $NH_3-N$ concentrations were significantly higher (p < 0.05) in 70F group at 09:30 and 10:30 h. Blood urea nitrogen was significantly higher (p < 0.05) in 70F group. It was concluded that BIRG based diet with a high forage level had no adverse effects on rumen fermentation, some blood chemical parameters, and immune system in dry Holstein cows and could be used as a forage source instead of imported TF.

Comparing the Effects of Manual and Self-exercise Therapy for Improving Forward Head Posture

  • Gyeongseop Sim;Donghoon Kim;Hyeseon Jeon
    • Physical Therapy Korea
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    • v.30 no.3
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    • pp.184-193
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    • 2023
  • Background: Studies investigating the immediate effects of a single intervention to correct forward head posture are rare. Objects: This study aimed to compare the changes in treatment effects in patients with forward head posture and neck pain after manual and self-exercise therapy over a 1-hour period. Methods: Twenty-eight participants were randomly divided into manual and self-exercise therapy groups. Following the initial evaluation, manual or self-exercise therapy was applied to each group for 30 minutes each in the prone, supine, and sitting positions. The variables measured were the craniovertebral angle (CVA), stress level, pain level, and sternocleidomastoid (SCM) stiffness. After the intervention, re-evaluation was conducted immediately, 30 minutes later, and 1 hour later. Two-way analysis of variance (ANOVA) was used to compare the maintenance of treatment effects between the two groups. Results: Based on the two-way mixed ANOVA variance, there was no interaction between the groups and time for all variables, and no main effects were found between the groups. However, a significant effect of time was observed (p < 0.05). Post hoc tests using Bonferroni's correction revealed that in both groups, the CVA, pain, and stress showed significant improvements immediately after the intervention compared with before the intervention, and these treatment effects were maintained for up to 1 hour after the treatment (p < 0.0083) in the manual therapy group. However, the stress level was maintained until 30 minutes later (p < 0.0083) in the self-exercise group. There was no significant decrease in right SCM stiffness before and after the intervention; however, left SCM stiffness significantly decreased after the self-exercise intervention (p < 0.0083). Conclusion: Both manual and self-exercise therapy for 30 minutes were effective in reducing forward head posture related to the CVA, pain, and stress levels. These effects persisted for at least 30 minutes.

Screening and Characterization of the High-Alcohol Producing Saccharornyces cerevisiae Dl (고농도 알콜발효효모 Saccharomyces cerevisiae D1의 분리 및 특성)

  • 양지영;박경호;백운화;유주현
    • Microbiology and Biotechnology Letters
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    • v.18 no.5
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    • pp.511-516
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    • 1990
  • A high-alcohol producing yeast strain had sugar and alcohol tolerance was isolated from soil and identified as Saccharomyces cerevisiae Dl according to the Lodder's yeast taxonomic studies. On investigation of the characteristics of the strain, it could grow in 60% glucose, within 15% ethanol and in the YPD medium containing 2.0 mM copper. It had 39.1% the inhibition rate of fermentation efficiency and 8% viability after 2 days in the YPD medium containing 15% ethanol. Its optimum initial pH, growth temperature, initial glucose concentration for the production of alcohol showed pH 4.5, $30^{\circ}C$, and 30%, respectively. Saa:hwomyce8 mvisiae Dl produced 14.0% (wlv) alcohol when incubated at $30^{\circ}C$, with orbital shaking 150 rpm for 72 h in a medium (pH 4.5) containing 30% (wfv) glucose.

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IMMUNOHISTOCHEMICAL STUDY OF P21 AND P53 EXPRESSION IN AMELOBLASTOMA (법랑아세포종에서 p21 및 p53 발현에 관한 면역조직화학적 연구)

  • Shin, Dong-Joon;Myoung, Hoon;Hwang, Kyeng-Kyun;Kim, Myung-Jin
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.29 no.4
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    • pp.199-205
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    • 2003
  • The p53 protein was discovered in 1979 as cellular 53-kD nuclear phosphoprotein bound to the large transforming antigen of SV40 virus. $P21^{WAF1/CIP1}$, which has been described as the critical downstream mediator of p53, is known to suppress DNA replication and arrest the G1 cell cycle by quaternary complex with cyclin D, cyclin-dependent kinase(CDK) and proliferating cell nuclear antigen(PCNA). In these days, some studies shows that the p21 can be induced by independent pathways. There are various reports about the expression of p21 (67%.82.4%) in oral squamous cell carcinoma. But these studies are mostly done in malignant tumor not in benign tumor. So we decided to study the expression of p21 in ameloblastoma and the relationship between p53 and p21 as a downstream mediator of p53 in ameloblastoma. We investigated the expression of p21 and p53 with the method of immunohistochemistry. We selected 30 cases of ameloblastoma tissue blocks (acanthomatous type: 5 cases, follicular type: 8 cases, plexiform type: 17 cases) imbedded in paraffin. We used 30 cases of normal gingival tissues and 30 cases of squamous cell carcinoma tissues (SCC) respectively and compared their results with those of ameloblastoma. We made slides with the streptavidin-biotin methods and used monoclonal antibody DO-7 (Novocastra, Newcastle, United Kingdom) as p53 antibody and monoclonal antibody M7202 (DAKO, California, U.S.A.) as p21 antibody. We used Pearson's correlation coefficient to analyse the relationship. The results were as follows: 1. p21 was expressed in ameloblastoma about 30% and this is lower than that of normal gingiva and SCC. 2. In normal gingiva and ameloblastoma, p21 expression was correlated with p53 expression. 3. In SCC, p21 were expressed about 83.3% and this is more than that of p53. But there was no correlation between p21 and p53 expression. We confirmed p21 expression and relation with p53 in ameloblastoma. But, to confirm the function of p21, more studies about p21 expression in malignant ameloblastoma and ameloblastic carcinoma are needed.