• Journal of Microbiology
• /
• v.39 no.1
• /
• pp.79-82
• /
• 2001

The influence of levulinic acid (LA) on the production of copolyester consisting of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) by Ralstonia eutropha was investigated. Addition of LA into the culture medium greatly increased the molar fraction of 3HV in the copolyester, indicating that LA can be utilized as a precursor of 3HV. In shake flask culture, the 3HV content in the copolyester increased from 7 to 75 mol% by adding 0.5 to 4.0 g/L LA to the medium containing fructose syrup as a main carbon source. A maximal copolyester concentration of 3.6 g/L (69% of dry cell weight) was achieved with a 3HV content of 40 mo1% in a jar fermentor culture containing 4.0 g/L of LA. When LA (total concentration, 4 g/L) was added repeatedly into a fermentor culture to maintain its concentration at a low level, the copolyester content and the 3HV yield from LA reached up to 85% of dry cell weight and 5.0 g/g, respectively, which were significantly higher than those when the same concentration of the LA was supplied al1 at once. The present results indicated that LA is more effective than propionate or valerate as a cosubstrate fur the production of copolyesters with varying molar fractions of 3HV by R. eutropha.

• Bulletin of the Korean Chemical Society
• /
• v.35 no.5
• /
• pp.1317-1322
• /
• 2014

Membrane disruption by an antimicrobial peptide (AMP) was investigated by measuring the $^2H$ solid-state nuclear magnetic resonance spectra of 1-palmitoyl-$d_{31}$-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC_$d_{31}$) in mixtures of POPC_$d_{31}$/cholesterol and either magainin 2 or aurein 3.3 deposited on thin cover-glass plates. The line shapes of the experimental $^2H$ solid-state nuclear magnetic resonance (SSNMR) spectra were best simulated by assuming the coexistence of a mosaic spread of bilayers containing pore structures and a fasttumbling isotropic phase or a hexagonal phase. Within a few days of incubation in a hydration chamber, an isotropic phase and a pore structure were induced by magainin 2, while in case of aurein 3.3 only an isotopic phase was induced in the presence of a bilayer phase. After an incubation period of over 100 days, alignment of the bilayers increased and the amount of the pore structure decreased in case of magainin 2. In contrast with magainin 2, aurein 3.3 induced a hexagonal phase at the peptide-to-lipid ratio of 1/20 and, interestingly, cholesterol was not found in the hexagonal phase induced by aurein 3.3. The experimental results indicate that magainin 2 is more effective in disrupting lipid bilayers containing cholesterol than aurein 3.3.

• Journal of Institute of Control, Robotics and Systems
• /
• v.22 no.3
• /
• pp.186-191
• /
• 2016

This paper addresses the development of 3D data acquisition system (3D scanner) based laser structured-light image. The 3D scanner consists of a stripe laser generator, a conventional camera, and a rotation table. The stripe laser onto an object has distortion according to 3D shape of an object. By analyzing the distortion of the laser stripe in a camera image, the scanner obtains a group of 3D point data of the object. A simple semiconductor stripe laser diode is adopted instead of an expensive LCD projector for complex structured-light pattern. The camera has an optical filter to remove illumination noise and improve the performance of the distance measurement. Experimental results show the 3D data acquisition performance of the scanner with less than 0.2mm measurement error in 2 minutes. It is possible to reconstruct a 3D shape of an object and to reproduce the object by a commercially available 3D printer.

• Journal of Institute of Control, Robotics and Systems
• /
• v.22 no.10
• /
• pp.811-816
• /
• 2016

In light of recently developed 3D printers for rapid prototyping, there is increasing attention on the 3D scanner as a 3D data acquisition system for an existing object. This paper presents a prototypical 3D scanner based on a striped laser light image. In order to solve the problem of shadowy areas, the proposed 3D scanner has two cameras with one laser light source. By using a horizontal rotation table and a rotational arm rotating about the latitudinal axis, the scanner is able to scan in all directions. To remove an additional optical filter for laser light pixel extraction of an image, we have adopted a differential image method with laser light modulation. Experimental results show that the scanner's 3D data acquisition performance exhibited less than 0.2 mm of measurement error. Therefore, this scanner has proven that it is possible to reconstruct an object's 3D surface from point cloud data using a 3D scanner, enabling reproduction of the object using a commercially available 3D printer.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 2001.11a
• /
• pp.103-103
• /
• 2001

인간유방상피세포에서 H-ras가 침윤성과 세포 이동성을 유도한다는 것을 이 전연구에서 밝혔다. Phosphatidylinositol 3-kinase(PI3K)는 세포 이동성에서 중요한 역할을 하는 것으로 보고되고 있다. 본 연구에서 인간유방상피세포인 MCF10A에서 H-ras에 의해 유도된 침윤성에 PI3K가 어떠한 영향을 미치는지 살펴보고자 하였다. PI3K의 활성은 PI3K의 downstream molecule인 Akt의 인산화를 Western blot으로 확인하였다. Akt는 MCF10A, H-ras, N-ras MCF10A 세포에서 같은 정도로 발현되는 반면, 인산화된 Akt는 MCF10A 세포에 비해 H-ras MCF10A 세포와 N-ras MCF10A 세포에서 현저히 높게 나타났다. 이상의 결과로서 H-ras, N-ras 둘 다 PI3K를 활성화시키며, 침윤성과 세포이동성이 없는 N-ras MCF10A 세포에서도 PI3K가 활성화되었으므로, PI3K의 활성은 세포침윤성과 이동성을 유도하는데에 있어서 충분하지는 않음을 말해준다. PI3K의 저해제인 LY294002와 wortmannin을 세포에 처리하였을 때 세포침윤성과 이동성이 유의성 있게 감소되었다. 이상의 결과는 MCF10A 세포의 침윤성과 이동설에 있어서 PI3K의 활성이 충분하지는 않지만 반드시 필요하다는 것을 알 수 있었다.

• Korean Journal of Crystallography
• /
• v.1 no.2
• /
• pp.66-75
• /
• 1990

The Pd-Te system has been investigated by differential thermal analysis, X-ray diffration, electron probe microanalysis and reflected light microscopy. New phase relations in 0-50at% Te portion of the binary system are proposed. Eight binary phases exist in the system:Pd17Te4, Pd20Te7.PdsTe3, P477e3, P497e4, P637e2, PeTe and PaTe2. Of these, P677e3 is a newly reported phase. P4177e4 is cubic, space group Fd3c, with a=12.678(5)A. The X-ray powder data of PdsTe3, indexed on an orthorhombic cell, give a= 12.843(3), b=15.126(3), c: 11.304(2)A and those of PdTTe.1, indexed on a monoclinic cell, give a=7.444(1), b= 13.918(2) , c=8.873(2)A. p =92.46(2). Some physical and optical properties of synthetic phases in the system are also reported.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.37 no.6
• /
• pp.1109-1117
• /
• 2017

Recently, 3D printer technology has been attracting attention in various industrial fields, and research papers are being conducted to utilize 3D printers in the construction industry. Actual structures such as bridges and buildings are being printed to 3D printers, and various applications such as mock-up structures using 3D printers are being proposed. In order to utilize 3D printer technology in the construction field, a 3D model is required, and the 3D BIM data produced at the design stage can be printed by a 3D printers, saving the cost and time of 3D model generation. However, 3D BIM data often does not satisfy the conditions for 3D printer output, causing many errors on output. In this paper, authors propose a problem analysis for 3D BIM model output to 3D printer and a method for reducing errors in 3D printing process of 3D BIM model. In addition, this paper presents a practical application of 3D model output from 3D printer.

• The Research Journal of the Costume Culture
• /
• v.24 no.6
• /
• pp.725-742
• /
• 2016

In the Korean fashion industry, 3D printing systems are considered as new technology and a new opportunity. With 3D printers, consumers can be manufacturers and individuals can develop businesses with little upfront capital. In this study, a dress form for the typical Korean women's body shape was developed using 3D technology (3D scanning, 3D modeling, and 3D printing). Ten women with apparel sizes 85-91-160 were selected from 3D body-scan data collected by SizeKorea of 201 women aged 25 to 34 (2010). First, 15 horizontal cross-sections were collected from the 3D scan data of the 10 subjects. Then, inside lines of those cross-sections were drawn at 15-degree intervals, and the lengths were measured. The average of the inside lines was connected to the internal spline curve, and the curves were used as the average cross-sections. The average torso body and the dress form of Korean women were developed into a 3D solid model using a 3D CAD program (Solidworks 2012). An output mockup was printed by the FDM type's 3D printing system (Bonbot 1200, Bonbot 3-H4) using PLA material. The dimensions comparing the 3D solid modeling to the 3D printed mockup of the dress form were measured, and minor differences were between 0.00cm and 0.40cm. In the future, 3D printing systems are expected to be in use for various personalized dress forms.

• Journal of the Korean Chemical Society
• /
• v.36 no.5
• /
• pp.644-651
• /
• 1992

The kinetics of the gas phase reaction of ozone(∼0.5 torr) with sulfur trioxide was investigated in the range of 6∼12 torr pressure at 69∼150${\circ}C$. The reaction rate of ozone with sulfur trioxide was faster than the reaction rate of $O_3 in the presence of CO_2 alone. No evidence for a molecular reaction of O_3 with SO_3 was found and the faster rate is probably due to impurity (HX) from the SO_3 reactant which gives rise to a chain reaction initiated by O_3 ＋ HX → OH ＋ O_2 ＋ X and also SO_3 has a larger collision diameter, which may be attributed to the O3 thermal decomposition more feasibly. The proposed experimental law [-d(O_3)/dt] = k_a(SO_3)(O_3) ＋ k_b(O_3)^{3/2} gives a rate constant ka(M-1 s-1) = (1.55 {\pm} 0.67) {\times} 105 e-{(9.27 0{\pm}0.43)kcal/RT}.$

• Archives of Pharmacal Research
• /
• v.18 no.6
• /
• pp.449-453
• /
• 1995

The search for patinum (II)-based compounds with improved therapeutic properties was prompted to design and synthesize a new family of water-soluble, third generation cis-diamminedichlorplatinum (II) complexes linked to uracil and uridine. Six heretofore undescribed uracil and uridine-platinum (II) complexes are ; [N-(2-aminoethyl)uracil-5-carboxamide]dichloroplatinum (II)(3a), [N-2(2-aminoethyl)uracil-6-carboxmide]dichloroplatinum (II) (3b),[5-(2-aminorthyl)carbamoyl-2',3',5',-tri-O-acetyluridine] dichloroplatinum (II) (6b), [5-(2-aminoethyl)-carbamoyl]-2',3',5',-tri-O-acetyluridine] dichloroplatinum (II) (6b), [5-(2-aminoethyl)carbamoylu-ridine]dihloroplatinum (II) (7a), [6-(2-aminoethyl)carbamoyluridine]dichloroplatinum (II) (7b). These analogues were prepared from the key starting materials, 5-carboxyuracil (1a) and 6-carboxyuracil (1b) which were reacted with ethylenediamine to afford the respective N-(2-aminoethyl)uracil-5-carboxmide (2a) land N-(2-aminoethyl)uracil-6-carboxamide (2b). The cisplatin complexes 3a and 3b were obtained through the reaction of the respective 2a and 2b ficiently introduced on the .betha.-D-ribose ring via a Vorbruggen-type nucleoside coupling procedure with hexamethyldisilazane, trimethylchlorosilane and stannicchloride under anhydrous acetonitfile to yield the sterospecific .betha.-anomeric 5-carboxy-2',3',5'-tri-O-acetyluridine (4a) and 6-carboxy-2',3',5'-tri-O-acetyluridine (4b), respective 5-(2-aminoethyl)carbamoyl-2',3',5'-tri-O-acetyluridine (5a) and 6-(2-aminoethyl)carbamoyl-2',3',5'-tri-O-acetyluridine (5b). The diamino-uridines 5a and 5b were reacted with potassium tetrachloroplatinate (II) to give the novel nucleoside complexes, 6a and 6b respectively which were deacetylated into the free nucleosides, 7a and 7b by the treatment with CH/sub 3/ONa. The antitumor activities were evaluated against three cell lines (K-562, FM-3A and P-388).