• Journal of Life Science
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• v.30 no.4
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• pp.321-330
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• 2020

The aim of this study was to investigate the effect of copper (II) chloride (CuCl₂) on zebrafish. Zebrafish were exposed to various CuCl₂ concentrations and subjected to different exposure times to determine the median lethal concentration (LC₅₀) values. To evaluate stress responses, we measured whole-body cortisol levels and behavioral parameters using the open field test (OFT) or the novel tank test (NTT). The zebrafish were exposed to CuCl₂ solution at concentrations of 1.5-150 ㎍/l or a vehicle for 1 hr before behavioral tests or sample collection for whole-body cortisol. The LC₅₀ values were 30.3, 25.3, and 14.8 ㎍/l at 24, 48, and 96 hr, respectively. The NTT showed that mobility, velocity, and distance covered were significantly lower in zebrafish exposed to CuCl₂ than in the control group (p<0.05), while the turn angle was significantly higher in zebrafish exposed to a CuCl₂ concentration of 150 ㎍/l than in the control group (p<0.05). The OFT also showed that mobility, velocity, and distance covered were significantly lower and the turn angle and meandering were significantly higher in zebrafish exposed to all concentrations of CuCl₂ than in the control group (p<0.05). The whole-body cortisol levels were significantly higher in zebrafish exposed to CuCl₂ than in the control group (p<0.05). These results suggest that exposure to lethal CuCl₂ concentrations induces an intense toxic and stress response in zebrafish, causing behavioral changes and increasing whole-body cortisol levels.

• Bulletin of the Korean Chemical Society
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• v.20 no.10
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• pp.1200-1204
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• 1999

The reactions of Ru(tpy)Cl3 (tpy = 2,2';6',2"-terpyridine) with new N,N,C-terdentate ligands, 3,2'-annulated-6-(2"-pyridyl)-2-phenylpyridines (1, HL) and the properties of their Ru(II) complexes are described. The distribution ratio of the two possible Ru(II) complexes, a pentaaza-coordinated complex [Ru(tpy)(1-N,N')Cl]+ and a cycloruthenated complex [Ru(tpy)(La-N,N',C)]+ are highly dependent on the length of the polymethylene unit. The trimethylene bridge of the N,N,C-terdentate in pentaaza-coordinated complex is rigid enough to induce an asymmetry in the complex.

• Journal of the Korean Institute of Landscape Architecture
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• v.45 no.3
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• pp.1-8
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• 2017

The present study aimed to investigate the effect of calcium chloride($CaCl_2$) on the growth and physiological responses of Pinus strobus and the variables that are sensitive to $CaCl_2$. Thus, changes in the visible damage, growth of root collar diameter, plant water content, chlorophyll content and composition, maximum PS II photochemical efficiency, and electron transport rate of P. strobus was analyzed in relation to treatment witih $CaCl_2$. A $CaCl_2$ solution(0.5, 1.0 and 3.0%) was applied in the root zone before leaf unfolding. Leaf browning, defoliation, and drying were observed with $CaCl_2$ application and this pattern was aggravated as the $CaCl_2$ concentration increased and the treatment period became longer. The decrease of growth in root collar diameter and height and leaf water content were observed at $CaCl_2$ 1.0% and 3.0%. The total chlorophyll content indicated that photopigment, PS II photochemical efficiency and electron transport rate significantly decreased at $CaCl_2$ 3.0%. In conclusion, $CaCl_2$ affected leaf water content and led to a decrease of capability in light harvesting and photochemical responses. Also, as a result of the correlation between calcium chloride concentration and growth and physiological response parameters, it was found that the leaf moisture content and the ratio of chlorophyll a and b reflect the damage level of calcium chloride sensitively because their coefficient of determinations were relatively high.

• Membrane Journal
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• v.29 no.3
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• pp.140-146
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• 2019

In this study, a selective layer of poly styrene sulfonic acid (PSSA) and polyethyleneimine (PEI) was formed by layer-by-layer method onto a porous polyacrylonitrile (PAN) hollow fiber membrane as the suppoter membrane. The salting out method was used by adding Mg salt to the coating solution. Several experimental conditions of the ionic strength, polymer concentration, and coating time were investigated, and the flux and rejection were measured at the operating pressure of 2 atm for 100 mg/L of NaCl, $MgCl_2$, and $CaSO_4$ as the feed solution. The membranes coated with PSSA 20,000 ppm, coating time 3 minutes, ionic strength 1.0, PEI 30,000 ppm, coating time 1 minute, and ionic strength 0.1 were observed the best. In the 100 ppm NaCl, $MgCl_2$, and $CaSO_4$ feed solutions, the flux of 20.4, 19.4, and 18.7 LMH, and the rejection of 67, 90, and 66.6%, respectively.

• Molecules and Cells
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• v.26 no.5
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• pp.468-473
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• 2008

Low-density lipoprotein (LDL) induces cell proliferation in human aortic smooth muscle cells (hAoSMCs), which may be involved in atherogenesis and intimal hyperplasia. Recent studies have demonstrated that $Cl^-$ channels are related to vessel cell proliferation induced by a variety of stimuli. In this study, we investigated a potential role of $Cl^-$ channels in the signaling pathway of LDL effects on hAoSMC proliferation with a focus on the activation of Erk1/2-PI3K/Akt and the subsequent upregulation of Egr-1. $Cl^-$ channel blockers, DIDS, but neither NPPB nor Furosemide, completely abolished the LDL-induced DNA synthesis and cell proliferation. Moreover, DIDS, but not NPPB, significantly decreased LDL-stimulated $Cl^-$ concentration, as judged by flow cytometry analysis using MQAE as a $Cl^-$-detection dye. DIDS pretreatment completely abolished the activation of Erk1/2 and PI3K/Akt in a dose-dependent manner that is the hallmark of LDL activation, as judged by Western blot and proliferation assays. Moreover, pretreatment with DIDS ($Cl^-$ channel blockers) but not LY294002 (PI3K inhibitors) completely abolished the LDL-induced upregulation of Egr-1 to the same extent as PD98059 (MEK inhibitors to inhibit Erk), as judged by Western blot and luciferase reporter assays. This is the first report, to our knowledge, that DIDS-sensitive $Cl^-$-channels play a key role in the LDL-induced cell proliferation of hAoSMCs via the activation of Erk1/2 and PI3K/Akt and the upregulation of Egr-1.

• Polymer(Korea)
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• v.36 no.1
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• pp.111-116
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• 2012

A functionalization of mesoporous materials with organosilane was carried out via a post-synthesis grafting method and $(n-BuCp)_2ZrCl_2$/methylaluminoxane (MAO) as subsequently immobilized on the functionalized mesoporous materials for ethylene polymerization. Organosilanes having amine, cyano or imidazoline group such as $N$-[(3-trimethoxysilyl)propyl]ethylenediamine (2NS), 4-(triethoxysilyl)butyronitrile (1NCy), 1-(3-triethoxysilylpropyl)-2-imidazoline (2NIm) were used for the surface functionalization of mesoporous materials. In the SBA-15/2NS/$(n-BuCp)_2ZrCl_2$ catalyst preparation, the amount of MAO in feed increased with an decrease in the Zr content of the supported catalyst, and Al content in the supported catalyst increased. The ethylene homopolymerization activity of SBA-15/2NS/$(n-BuCp)_2ZrCl_2$ dramatically increased as the amount of MAO in feed increased. Furthermore, when the immobilization time was 6 hrs, SBA-15/2NS/$(n-BuCp)_2ZrCl_2$ showed the highest activity. The activities of supported 2NS-, 1NCy-, 2NIm-functionalized catalysts decreased in the following order, SBA-15/2NS/ > SBA-15/2NIm/ > SBA-15/1NCy/$(n-BuCp)_2ZrCl_2$. 2NS and 2NIm which have two amine groups per silane molecule were shown to interact with $(n-BuCp)_2ZrCl_2$ strongly compared to 1NCy which has one amine group. Thus, the activities increased with an increase in the nitrogen and the Zr content of the supported catalysts.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.6
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• pp.442-446
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• 2007

From the rhizoplane and rhizosphere of pepper, tomato, lettuce, pasture, and grass, unsoluble inorganic phosphate solubilizing bacterial strains were isolated using plate base assay on Pikovskaya's medium. Two strains, CL-1 and CL-2, which produced largest halo on plates (indicative of phosphate solubilization)were selected for further studies. Based on these biochemical and 16S rRNA analysis strains CL-1, CL-2 were found to be as species of Pseudomonas sp. and Kluyvera sp., respectively. In broth assay Pseudomonas sp. CL-1 and Kluyvera sp. CL-2 solubilized insoluble phosphate by 193.4 mg and $493.6P\;mg\;L^{-1}$, respectively after $3^{rd}$ day inoculation. These effecient phosphate solubilizing bacteria have a potential to be developed as microbial based fertilizer in future.

• Journal of the Korean Chemical Society
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• v.25 no.1
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• pp.7-12
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• 1981

Solvolysis of $p-CH_3, p-H, p-Cl and p-NO_2$-benzenesulfonyl chlorides have heen studied in MeOH-MeCN mixtures. A nonlinear Hammett plot with a ratio order of p-NO_2 > p-CH_3 > p-H > p-Cl was obtained; the reaction was thought to proceed by an S_N1-S_N2 borderline mechanism. In all cases the reactivity was a maximum at 90∼95%(v) methanol, whereas methanol monomer selectivity defined as fi = \frac{k1}{ki}i (k1; observed pseudo-first order rate constant: ki; hypothetical rate constant for MeOH solution having the same polymer structure as in the pure MeOH) was a maximum at 80% methanol with a decreasing order of fi as p-NO_2 > p-Cl > p-H > p-CH_3.$ This was interpreted as the decrease in tightness of transition state; the larger the fi, the tighter is the MeOH attached to the substrate, and hence the more susceptible the substrate becomes to the approaching monomer methanol.

• Journal of environmental and Sanitary engineering
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• v.16 no.4
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• pp.31-37
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• 2001

Deicers have used for melting snow on the road during winter season, but they have largely influenced on environment and public assets and human health. The pollution level of snow and soil contained deicer was analyzed and evaluated the characteristics of deicers. The results were as follows 1. In the result of measurement of pollutants in snow contained deicer, the pH was a little higher than a comparative group and the concentration of $Cl^{-}$ ranged from 0.5% to 0.87%, and the electric conductivity ranged 12.4 to 24.6 mmho/cm. The concentration of Cd, As, and Hg was not detected, but those of Cu, Pb, Cr was higher than a comparative group. 2. In soil of the road spreaded with deicer, the pH is getting alkalized and the concentration of $Cl^{-}$ was high in January and returned the level of a comparative grout) in November by physical and chemical reaction with deicer, but the concentration of heavy metals were not connected with deicers. 3. In comparison of deicing efficiency, the concentration of $Cl^{-}$ was 3.3~5times high in spreading with deicer before snowing than after snowing. The concentration of $Cl^{-}$ in NaCl was higher than $CaCl_2$, but the deicing efficiency of Nacl was better than that of $CaCl_2$. Moreover, the new deicer have no salt, but deicing efficiency of new deicer was less than that of NaCl and $CaCl_2$.

• Korean Journal of Crystallography
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• v.8 no.2
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• pp.124-126
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• 1997

The comples of [Ni(L)](ClO4)2 (L:3,5,10,12-tetramethyl-1,4,8,12-tetraazacyclotetradecane), C14H32Cl2N4NiO8, has a NiN4 square planar geometry and adopts a thermodynamically most stable trans-III conformation.