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The Interleukin-18 Promoter -607C>A Polymorphism Contributes to Nasopharyngeal Carcinoma Risk: Evidence from a Meta-analysis Including 1,886 Subjects

  • Guo, Xu-Guang;Xia, Yong
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권12호
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    • pp.7577-7581
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    • 2013
  • The interleukin-18 promoter -607C>A gene polymorphism may be related to nasopharyngeal carcinoma (NPC) risk but the results of individual studies remain conflicting. A meta-analysis including 1,886 subjects from five individual studies was therefore performed to provide a more accurate estimation. Pooled odds ratios (ORs) and their corresponding 95% confidence intervals (95% CIs) were evaluated by fixed- or random-effects models. A significant relationship between interleukin-18 promoter -607C>A gene polymorphism and NPC was found in a dominant genetic model (OR: 1.351, 95% CI: 1.089-1.676, P=0.006, $P_{heterogeneity}$=0.904), a homozygote model (OR: 1.338, 95% CI: 1.023-1.751, P=0.034, $P_{heterogeneity}$=0.863), and a heterozygote model (OR: 1.357, 95% CI: 1.080-1.704, P=0.009, $P_{heterogeneity}$=0.824). No significant association was detected in either an allelic genetic model (OR: 1.077, 95% CI: 0.960-1.207, 0.207, $P_{heterogeneity}$=0.844) or a recessive genetic model (OR: 1.093, 95% CI: 0.878-1.361, P=0.425, $P_{heterogeneity}$=0.707). In conclusion, a significant association was found between interleukin-18 promoter -607C>A gene polymorphism and NPC risk. Individuals with the C allele of interleukin-18 promoter -607C>A gene polymorphism have a higher risk of NPC development.

CYP1A1 (Ile462Val), CYP1B1 (Ala119Ser and Val432Leu), GSTM1 (null), and GSTT1 (null) Polymorphisms and Bladder Cancer Risk in a Turkish Population

  • Berber, Ufuk;Yilmaz, Ismail;Yilmaz, Omer;Haholu, Aptullah;Kucukodaci, Zafer;Ates, Ferhat;Demirel, Dilaver
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권6호
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    • pp.3925-3929
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    • 2013
  • We aimed to investigate bladder cancer risk with reference to polymorphic variants of cytochrome p450 (CYP) 1A1, CYP1B1, glutathione S-transferase (GST) M1, and GSTT1 genes in a case control study. Polymorphisms were examined in 114 bladder cancer patients and 114 age and sex-matched cancer-free subjects. Genotypes were determined using allele specific PCR for CYP1A1 and CYP1B1 genes, and by multiplex PCR and melting curve analysis for GSTM1 and GSTT1 genes. Our results revealed a statistically significant increased bladder cancer risk for GSTT1 null genotype carriers with an odds ratio of 3.06 (95% confidence interval=1.39-6.74, p=0.006). Differences of CYP1A1, CYP1B1 and GSTM1 genotype frequencies were not statistically significant between patients and controls. However, the specific combination of GSTM1 null, GSTT1 null, and CYP1B1 codon 119 risk allele carriers and specific combination of GSTM1 present, GSTT1 null, and CYP1B1 432 risk allele carriers exhibited increased cancer risk in the combined analysis. We did not observe any association between different genotype groups and prognostic tumor characteristics of bladder cancer. Our results indicate that inherited absence of GSTT1 gene may be associated with bladder cancer susceptibility, and specific combinations of GSTM1, GSTT1 and CYP1B1 gene polymorphisms may modify bladder cancer risk in the Turkish population, without any association being observed for CYP1A1 gene polymorphism and bladder cancer risk.

Genetic Polymorphism of Interleukin-1A (IL-1A), IL-1B, and IL-1 Receptor Antagonist (IL-1RN) and Prostate Cancer Risk

  • Xu, Hua;Ding, Qiang;Jiang, Hao-Wen
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권20호
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    • pp.8741-8747
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    • 2014
  • Purpose: We aimed to investigate the associations between polymorphisms of interleukin-1A (IL-1A), IL-1B, and IL-1 receptor antagonist (IL-1RN) and prostate cancer (PCa) risk. Materials and Methods: A comprehensive search for articles of MEDLINE and EMBASE databases and bibliographies of retrieved articles published up to August 3, 2014 was performed. Methodological quality assessment of the trials was based on a standard quality scoring system. The meta-analysis was performed using STATA 12.0. Results: We included 9 studies (1 study for IL-1A, 5 studies for IL-1B, and 3 studies for IL-1RN), and significant association was found between polymorphisms of IL-1B-511 (rs16944) as well as IL-1B-31 (rs1143627) and PCa risk. IL-1B-511 (rs16944) polymorphism was significantly associated with PCa risk in homozygote and recessive models, as well as allele contrast (TT vs CC: OR, 0.74; 95%CI, 0.58-0.94; P=0.012; TT vs TC+CC; OR, 0.79; 95%CI, 0.63-0.98; P=0.033; T vs C: OR, 0.86; 95%CI, 0.77-0.96; P=0.008). The association between IL-1B-31 (rs1143627) polymorphism and PCa risk was weakly significant under a heterozygote model (OR, 1.35; 95%CI, 1.00-1.80; P=0.047). Conclusions: Sequence variants in IL-1B-511 (rs16944) and IL-1B-31 (rs1143627) are significantly associated with PCa risk, which provides additional novel evidence that proinflammatory cytokines and inflammation play an important role in the etiology of PCa.

OBTUSE MATRIX OF ARITHMETIC TABLE

  • Eunmi Choi
    • East Asian mathematical journal
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    • 제40권3호
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    • pp.329-339
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    • 2024
  • In the work we generate arithmetic matrix P(c,b,a) of (cx2 + bx+a)n from a Pascal matrix P(1,1). We extend an identity P(1,1))O(1,1) = P(1,1,1) with an obtuse matrix O(1,1) to k degree polynomials. For the purpose we study P(1,1)kO(1,1) and find generating polynomials of O(1,1)k.

감초경작지의 잡초방제를 위한 제초제 선발 (Response of Liquorice (Glycyrrhiza uralensis) to Several Soil- and Foliar-Applied Herbicides)

  • 김성문;오혜영;김용호;조준모;허장현;한대성
    • 농약과학회지
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    • 제4권4호
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    • pp.81-86
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    • 2000
  • 감초 경작지의 잡초를 방제할 수 있는 제초제를 선발하기 위하여 국내에서 시판중인 12종의 토양처리 및 경엽처리 제초제의 약효 약해를 실내실험을 통하여 검정하였고, 선발된 제초제의 약효 약해를 포장실험을 통하여 검정하였다. 토양처리 제초제인 pendimethalin(1,585 g a.i. $ha^{-1}$), simazine(1,000 g a.i. $ha^{-1}$), alachlor(5 g a.i. $ha^{-1}$), metolachlor (1,600 g a.i. $ha^{-1}$), ethalfluralin(1,050 g a.i. $ha^{-1}$)를 각각 처리한 결과, 제초제들은 바랭이와 피에 대해서는 60% 이상의 살초력을 나타낸 반면, 어저귀와 메밀에 대해서는 60% 이하의 살초력을 나타내었다. 그리고 alachlor, metolachlor, ethalfluralin은 감초에 대해 중정도의 약해를($25{\sim}40%$), pendimethalin, simazine은 낮은 약해를 나타내었다($10{\sim}17%$). 실내실험 결과를 바탕으로 pendimethalin과 simazine를 포장시험을 위한 제초제로 선발하였고, 약효 약해를 춘천 소재 강원도 농업기술원 포장에서 검정하였다. 제초제 처리 4주 후 pendimethalin과 simazine 처리구의 감초 생체중은 무처리구의 감초 생체중(0.79 g $plant^{-1}$)과 비교하여 차이가 없었다. 무처리구에 발생한 잡초의 총생체중은 $187g m^{-2}$이었던 반면 제초제 처리구에서는 잡초가 전혀 발견되지 않았다. 경엽처리 제초제인 dicamba(964 g a.i. $ha^{-1}$), 2,4-D(280 g a.i. $ha^{-1}$), mecoprop(2,500 g a.i. $ha^{-1}$), flazasulfuron(75 g a.i. $ha^{-1}$), imazaquin(800 g a.i. $ha^{-1}$'), bentazon(1,600 g a.i. $ha^{-1}$), pyribenzoxim(30 g a.i. $ha^{-1}$)을 각각 $3{\sim}4$엽기의 감초와 어저귀, 메밀, 바랭이, 피의 경엽에 처리하고 처리 7일 후 약효 약해를 실내 검정한 결과, dicamba, 2,4-D, mecoprop, bentazon은 감초 유식물에 대해 완전해를 나타낸 반면, flazasulfuron, imazaquin, pyribenzoxim은 30% 이하의 약해를 나타내었다. 감초에 대하여 낮은 약해를 나타낸 flazasulfuron, imazaquin, pyribenzoxim은 어저귀, 메밀, 바랭이, 피에 대하여 50% 미만의 약효를 나타내었기에 감초 경작지용 제초제로는 적합하지 않다고 판단되었다. 본 실험의 결과를 바탕으로 감초경작지용 제초제로는 토양처리용 pendimethalin과 simazine이 적합할 것이라 판단된다.

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에코나졸 나이트레이트의 구조 (The Structure of Econazole Nitrate)

  • 서일환;조성일;박권일
    • 한국결정학회지
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    • 제1권1호
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    • pp.14-18
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    • 1990
  • Econazole nitrate, 1-{2-[(4-chlorophenyl)methoxy]-2-(2,4-dichlorophenyl) ethy1}-1H-imidazole mono-nitrate, C18 H16 CI13 N3 O4의 단위세포 상수는 a=19.337(4) A, b=15.191(5) A c=7.601(3)A, β=91.72(2)μ V=2000.0A3 Do=1.49g/ml Dm=1.47g/ml, M=4.31cm-1 F(000)=912.0, 2T=298K,공간군은 P2₁/C이고 단사 정계이며 Z=4이다. λ=(Mo-Ka)=0.7107 A을 사용한 1330개의 Intensity data에 대해 최종 R값은 0.06이다. Econazole nitrate의 각 세 ring은 각각 평면이며 B,A,C ring순서로 층계를 이루고 있다. 반면에 Econazole의 A와 C ring은 거의 같은 평면에 누워 있으나 B ring 은 거의 같은 평면이며 B,A,C ring순서로 층계를 이루고 있다.반면에 Econazole의 A와 C ring은 거의 같은 평면에 누워있으나 B ring과는 약 60˚의 평면각을 이루고 있다. Nitrate의 O(*3)와 Imidaze의 N(2) 사이의 수소결합은 Econazole slt의 안정화에 기여한다.

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Kinesin superfamily KIF1A와 결합하는 미세소관 불안정화 단백질 SCG10의 규명 (SCG10, a Microtubule-Destabilizing Factor, Interacts Directly with Kinesin Superfamily KIF1A Protein in Brain)

  • 문일수;석대현
    • 생명과학회지
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    • 제19권7호
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    • pp.859-865
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    • 2009
  • 미세소관은 세포골격단백질의 중요한 구성 단백질로 축삭돌기 내에서는 세포막 방향으로 정렬되어 있다. Kinesin superfamily (KIFs)는 세포 내에서 미세소관을 따라 세포 내 소포들을 운반하는 분자 자동차 (molecular motor) 단백질이다. 본 연구에서 우리는 효모 two-hybrid system을 사용하여 KIF1A의 coiled-coil 영역과 결합하는 단백질로 미세소관 불안정화 요소인 SCG10 단백질을 분리하였다. SCG10은 KIFs에서 KIF1A와만 특이적으로 결한 하며, KIF1A의 400에서 820아미노산 부위가 SCG10과의 결합에 필수적임을 효모 two-hybrid assay로 확인하였다. 또한 SCG10의 coiled-coil영역은 KIF1A와의 결합에 필수영역임을 확인하였으며 단백질간의 결합은 Glutathione S-transferase pull-down assay를 통하여 확인하였다. 생쥐의 뇌 파쇄액에 SCG10항체로 면역침강을 행하여 KIF1A를 확인한 결과KIF1A는 SCG10과 특이적으로 같이 침강하였다. 이러한 결과들은 KIF1A는 SCG10와 결합하여 SCG10이 포함된 소포를 미세소관을 따라 이동시킴을 시사한다.

Misexpression of AtTX12 encoding a Toll/interleukin-1 receptor domain induces growth defects and expression of defense-related genes partially independently of EDS1 in Arabidopsis

  • Song, Sang-Kee
    • BMB Reports
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    • 제49권12호
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    • pp.693-698
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    • 2016
  • In this study, a tissue-specific GAL4/UAS activation tagging system was used for the characterization of genes which could induce lethality when ubiquitously expressed. A dominant mutant exhibiting stunted growth was isolated and named defective root development 1-D (drd1-D). The T-DNA tag was located within the promoter region of AtTX12, which is predicted to encode a truncated nucleotide-binding leucine-rich repeat (NLR) protein, containing a Toll/interleukin-1 receptor (TIR) domain. The transcript levels of AtTX12 and defense-related genes were elevated in drd1-D, and the misexpression of AtTX12 recapitulated the drd1-D phenotypes. In the presence of ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), a key transducer of signals triggered by TIR-type NLRs, a low-level of AtTX12 misexpression induced strong defective phenotypes including seedling lethality whereas, in the absence of EDS1, a high-level of AtTX12 misexpression induced weak growth defects like dwarfism, suggesting that AtTX12 might function mainly in an EDS1-dependent and partially in an EDS1-independent manner.

In situ PCR에 의한 alcelaphine herpesvirus-l (AHV-l)의 진단법 개발 및 다른 분자생물학적 진단법들과의 비교 (In situ PCR for the Detection of Alcelaphine Herpesvirus-l and Comparison with other Molecular Biological Diagnostic Methods)

  • 김옥진
    • 한국수의병리학회지
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    • 제6권1호
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    • pp.1-5
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    • 2002
  • A1celaphine herpesvirus 1 (AHV-1) is a causative agent of malignant catarrhal fever which is a fatal and a lymphoproliferative syndrome. AHV-1 is a gamma herpesvirus, which induces frequent latent infection and often difficult to detect its antigens or specific nucleic acids because of its low viral copies in the infected tissues. A new method, in situ PCR, is developed for the detection of AHV-1 nucleic acid in this study. Target sequences of AHV-1 open reading frame 50 gene were detected within AHV-1 infected MDBK cells. As compare with other molecular biological methods for the detection of AHV-1, in situ PCR was found to be more sensitive than in situ hybridization and to be less sensitive than nested PCR. However, nested PCR cannot afford to observe and differentiate AHV-1 infected cells. In situ PCR amplifies a target sequence within cells that can be visualized microscopically with increased sensitivity compared to detection by in situ hybridization. In situ PCR has wide applications for sensitive localization of low copy AHV-1 viral sequences within cells to investigate the role of viruses in a variety of clinical conditions and also provide the rapid, sensitive, and specific detection of AHV-1 infection.

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1q21.1 microdeletion identified by chromosomal microarray in a newborn with upper airway obstruction

  • Kim, Yoon Hwa;Yang, Ju Seok;Lee, Young Joo;Bae, Mi Hye;Park, Kyung Hee;Lee, Dong Hyung;Shin, Kyung-Hwa;Kim, Seung Chul
    • Journal of Genetic Medicine
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    • 제15권1호
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    • pp.34-37
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    • 2018
  • A 1q21.1 microdeletion is an extremely rare chromosomal abnormality that results in phenotypic diversity and incomplete penetrance. Patients with a 1q21.1 microdeletion exhibit neurological-psychiatric problems, microcephaly, epilepsy, facial dysmorphism, cataract, and thrombocytopenia absent radius syndrome. We reported a neonate with confirmed intrauterine growth restriction (IUGR), micrognathia, glossoptosis, upper airway obstruction, facial dysmorphism, and eye abnormality at birth as well as developmental delay at the age of 1 year. These clinical manifestations, except for the IUGR and upper airway obstruction, in the neonate indicated a 1q21.1 microdeletion. Here, we report a rare case of a 1q21.1 microdeletion obtained via paternal inheritance in a newborn with upper airway obstruction caused by glossoptosis and tracheal stenosis.