• Title/Summary/Keyword: A. rufa

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Useful Insects : Study on Behavior of Fruits Feeding and Ovipositional Characterization of Its Adult in Pylocoelia rufa (유용곤충 산업화를 위한 늦반딧불이 유충의 과일류에 대한 섭식행동과 성충의 산란특성 연구)

  • Lee, Hee-Kwon;Lee, Hoi-Seon
    • Korean Journal of Organic Agriculture
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    • v.24 no.4
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    • pp.859-867
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    • 2016
  • This study was conducted at rearing room to investigate the feeding behavior of fruits in the firefly (Pylocoelia rufa) larvae and the ovipositional characteristics of the adults. As a result, the pear, apple, melon, persimmon, mandarin, grape and peach were shown the feeding behavior of larvae the average 6.40, 3.25, 2.50, 2.20, 2.00, 1.40 and 1.25, respectively. However, the larvae were not shown at all feeding behavior to banana. The hours feeding behavior of firefly larvae was primarily from 8:00 pm until 2:00 am. In addition, the time at which the voracious feeding behavior was until 8:00 pm to 12:00 pm. The 6:00 pm began whisking all the larvae are hiding in the shelter. In the case of the firefly larvae fed only the edible snail, the adults were oviposition an average $49{\pm}6$ eggs. At that time larva stage, adults of the firefly eating the pears, apples, melon and eat edible snail at the same time there were oviposition the $53{\pm}7$ on average. In the case of fruits, the pears, apples, and melons has been considered worthy to take the firefly larva fed with the current land snails.

Bacteriological Study about the Death of Cultured Doctor Fish, Garra rufa in the Aquarium

  • Lee, Ji-Yoon;Gang, Nam-I;You, Jin-Sol;Ko, Chang-Yong;Lee, Ki-Won;Han, Won-Min;Kim, Eunheui
    • Journal of Marine Life Science
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    • v.1 no.1
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    • pp.18-24
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    • 2016
  • Since April 2012, doctor fish in the breeding tank and in the quarantine tank in Hanwha Aquaplanet Yeosu Aquarium have been dying, accompanied by diffuse bleeding around the mouth, in the chin, and at the bottom of the abdomen. In this study, the cause of death would be examined through the bacteriological study of doctor fish and the rearing water quality in the aquarium. The water quality and the bacterial counts of the rearing water in the exhibit tank and in the quarantine tank were analyzed once a week, starting from August to November 2014. Water quality was measured based on the following data: temperature was in the range of 24.5~26.8℃, pH at 6.77~7.94, DO at 6.15~8.61 ppm, ammonia at 0~0.93 ppm, nitrite at 0.009~0.075 ppm, and nitrate at 1.1~40.9 ppm. Studies revealed that the differences in these water quality factors were not related to the death of doctor fish. Bacterial counts in the rearing waters of Garra rufa slightly increased to 103~104 CFU/ml, just before the death of the doctor fish. Twelve strains of bacteria were isolated from the dead fish and rearing waters. The isolates were identified as Aeromonas veronii, Citrobacter freundii, Pseudorhodoferax aquiterrae, Shewanella putrefaciens, and Vibrio anguillarum on the basis of 16S rRNA gene sequences. The most dominant species was C. freundii, which showed medium sensitivity to florfenicol and norfloxacin, and was resistant to amoxacillin, doxycycline, oxytetracycline, tetracycline, and trimethoprim. Ten isolates were confirmed to be pathogenic to the doctor fish. Doctor fish infected with C. freundii and S. putrefaciens showed high mortality in the experimental groups. These results indicate that the variation in bacterial numbers in the rearing water was related to the death of doctor fish. C. freundii and S. putrefaciens were directly implicated in causing the death of doctor fish in the aquarium.