• Journal of Integrative Natural Science
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• v.7 no.4
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• pp.260-265
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• 2014

The aim of this study was to assess the in vitro potential of methanolic seed extract of Abelmoschus esculentus as a natural antioxidant. The DPPH activity of the Ethyl acetate soluble fraction (10, 20, 40, 80, and $160{\mu}g/mL$) was increased in a dose dependent manner, which was found in the range of 18.97-90.47% as compared to ascorbic acid 26.44-93.71%. The $IC_{50}$ values of Ethyl acetate soluble fraction (EAES) and ascorbicacid in DPPH radical scavenging assay were obtained to be 28.12 and $18.43{\mu}g/mL$, respectively. Measurement of polyphenol content of the EAES of A. esculentus seed was achieved using Folin-Ciocalteau reagent containing 53.80 mg/g of total phenolic content, which was found signicantly higher when compared to reference standard gallic acid. Similarly total flavonoids and proabthocyanidis of EAES and chloroform soluble fraction (CAES) were found significantly 147 mg/g and 14.24 mg/g respectively when both compared to reference standard quercetin. EAES exhibited high significant lipid peroxidation inhibition effects in a dose-dependent manner, with $IC_{50}$ values of $38.08{\mu}g/mL$, whereas, standard quercetin, with $IC_{50}$ value of $36.67{\mu}g/mL$. All extract/fractions showed dose dependent reducing power ability and these differences were statistically significant (p<0.001). The results obtained in this study clearly indicate that A. esculentus seed has a signicant potential to use as a natural antioxidant agent.

• Journal of Bio-Environment Control
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• v.21 no.3
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• pp.267-270
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• 2012

This study was conducted to investigate of germination, okra yield and plant growth according to sowing time in the field cultivation. The percentages of germination in okra seed according to sowing time were 4.4.% (15 April), 66.7% (30 April), 88.9% (15 May), and 100% (30 May). The highest germination rate (100%) was observed on 30 May sowing whereas the lowest germination rate (4.4%) was observed on 15 April sowing. Treatment on 30 April showed the best result compared with other treatments in the growth and development. The plant growth was 476cm in 30 April, 317cm in 15 May, and 271cm in 30 May treatment. The fruit yield significantly was affected by sowing time. In case of sowing time, 15 May sowing produced the highest yield (836 kg/10a) and 30 May sowing produced the lowest yield (507 kg/10a). Therefore, 15 May sowing is best for better growth and yield of okra.