• 한국발생생물학회지:발생과생식
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• 제26권2호
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• pp.71-77
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• 2022

In response to luteinizing hormone (LH), a higher concentration of progesterone (P4) is produced in luteal cells of corpus luteum (CL). Mitochondria are an essential cellular organelle in steroidogenesis. The specific engagement of the concept regarding mitochondrial shaping with early stages of steroidogenesis was suggested in reproductive endocrine cells. Although the specific involvement of GTPase dynamin-related protein 1 (Drp1) with steroidogenesis has been demonstrated in luteal cells of bovine CL in vitro, its actual relationship with ovarian steroidogenesis during the estrous cycle remains unknown. In this study, while Fis1 and Opa1 protein levels did not show significant changes during the estrous cycle, Drp1, Mfn1, and Mfn2 proteins exhibited relatively lower levels at proestrus than at estrus or diestrus. 3β-HSD showed higher levels at proestrus than at estrus or diestrus. In addition, Drp1 phosphorylation (s637) was higher in proestrus than in estrus or diestrus. Immune-positive cells for Drp1, pDrp1 (s637), and 3β-HSD were all localized in the cytoplasm of luteal cells in the CL. The immune-positive cells for 3β-HSD were more frequently seen in the CL at proestrus than at estrus or diestrus. Immunoreactivity for Drp1 in luteal cells at proestrus was weaker than that at estrus or diestrus. However, pDrp1 (s637) immune-positive cells were mostly detected in luteal cells at proestrus. These results imply that steroidogenesis (P4 production) in the CL is closely related to phosphorylation of Drp1 at serine 637. Taken together, this study presents evidence that Drp1 phosphorylation at serine 637 is an important step in steroidogenesis in the CL.

• Reproductive and Developmental Biology
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• 제34권2호
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• pp.95-101
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• 2010

Decorin (DCN) is a member of small leucine-rich proteoglycans which are ubiquitous components of the extracellular matrix. It regulates many physiological processes, such as matrix formation, collagen fibrillogenesis, angiogenesis, cancer growth, and cardiovascular diseases. It has been shown that DCN is expressed in the uterus during pregnancy and modulates implantation and decidualization for the establishment and maintenance of pregnancy in mice and humans. Expression of DCN in the uterine endometrium during pregnancy has not been investigated in pigs. Thus, this study investigated expression of DCN in the uterine endometrium during the estrous cycle and pregnancy in pigs. Uterine endometrial tissues were from day (D) 12 and 15 of the estrous cycle and D12, D15, D30, D60, D90, and D114 of pregnancy. Northern blot and real-time RT-PCR analyses showed that expression of DCN mRNA was detected throughout the estrous cycle and pregnancy with the highest levels during mid pregnancy. In situ hybridization analysis showed that DCN mRNA was localized to both luminal and glandular epithelia during the estrous cycle and pregnancy and also to chorionic membrane during mid pregnancy in pigs. To determine whether endometrial expression of DCN was affected by the somatic cell nuclear transfer (SCNT) procedure, DCN mRNA levels in the uterine endometrium from gilts with SCNT embryos on D30 of pregnancy were compared with those from gilts with normal embryos using real-time RT-PCR analysis. The result showed that DCN mRNA levels in the uterine endometrium were not significantly different between gilts with normal embryos and SCNT embryos. These results suggest that DCN may play an important role for endometrial tissue remodeling during mid pregnancy, and DCN expression is not affected by the SCNT procedure at the early stage of pregnancy in pigs.

• Reproductive and Developmental Biology
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• 제37권3호
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• pp.103-108
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• 2013

An uterus is female reproductive tract organ that affected estrus cycle. During a various changes occur at uterus in estrus cycle, one of them is body fluids secretion be called uterine fluid. Therefore, the objective of this study was to investigate the changes of protein patterns using two-dimensional gel electrophoresis in uterus fluids during the follicular and luteal phases in estrus cycle of pigs. In changes of protein spots were confirmed during the follicular and luteal phases. The 136 spots were expressed in follicular phase, the 57 spots of them showed reproducibility. On the other hand, the 140 spots were expressed in luteal phase, the 73 spots of them showed reproducibility. Also, spots expressed in follicular phase were number 69 and 94 spots and spots expressed in luteal phase only were number 156, 157, 184~187, 190 and 191 spots. The spots which of higher expression levels in the luteal phase than in follicular phase were number 76 and 79 spots. In conclusion, the spots expressed in follicular and luteal phases were confirmed with difference levels and these differences are function of RNA resolving, protein synthesis and cytoskeletal architecture.

• 한국발생생물학회지:발생과생식
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• 제17권4호
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• pp.441-449
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• 2013

Recent study showed that T cells in the immune organs and peripheral blood are influenced by estradiol, leading to a dysfunction of the immune system. However, little is known about the thymic-gonadal relationship during the estrous cycle in mouse. Therefore, the purpose of this study was to elucidate the mechanism by which a change in estradiol levels during the estrous cycle regulates the development of T cells in the mouse thymus. Six-week-old ICR mice were used and divided into four groups, including diestrous, proestrous, estrous, and metestrous. We first confirmed that ER-${\alpha}$ and - ${\beta}$ estrogen receptors were expressed in thymic epithelial cells, showing that their expression was not different during the estrous cycle. There was also no significant difference in thymic weight and total number of thymocytes during the estrous cycle. To determine the degree of thymocyte differentiation during the estrous cycle, we analyzed thymocytes by flow cytometry. As a result, the percentage of CD4+CD8+ double-positive (DP) T cells was significantly decreased in the proestrous phase compared to the diestrous phase. However, CD4+CD8- or CD4-CD8+ (SP) T cells were significantly increased in the proestrous phase compared to the diestrous phase. In addition, the percentage of CD44+CD25- (DN1) T cells was significantly decreased in the estrous phase compared to other phases, whereas the percentages of CD44+CD25+ (DN2), CD44-CD25+ (DN3), and CD44-CD25- (DN4) were not changed during the estrous cycle. These results indicate that the development of thymocytes may arrest in the DP to SP transition stage in the proestrous phase displaying the highest serum level of estradiol. This study suggests that a change in estradiol levels during the estrous cycle may be involved in the regulation of thymocyte differentiation in the mouse thymus.

• 한국가축번식학회지
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• 제18권2호
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• pp.121-126
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• 1994

This study was conducted to examine the condition of activation of the nuclear transplant bovine embryos. In vitro fertilized(IVF) and nuclear transplant embryos(NTs) were co-cultured with bovine oviduct epithelial tissue(BOET). NTs were treated with cycloheximide(CHXM) for 0 to 6 h after electrofusion to investigate the activation conditin of recipient ooplast. Then, the infljence of the CHXM treatment timing on the cleavage and development of NTs were investigated in relation to the nuclear transplant time. The cleavage rates of NTs were increased with the increasing time of the CHXM treatment from 0 to 6 h (54.7 to 91.3%, P<0.01). Similar trend was shown in the development into the morula or blastocyst stage, but very limitted. Activation of enucleated oocytes prior to fusion enhanced development of NTs compared with that post fustion. This result suggests that the frequency of activation of NTs can be greatly enhanced by treating with CHXM for 6 h. The result also suggests that if blastomeres of unknown cell cycle stage are used, activation of enucleated oocytes prior to fusion enhances development of NTs.

• ALGAE
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• 제23권2호
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• pp.141-150
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• 2008

Populations of Codium amplivesiculatum were found in association with rhodolith beds. We present population trends of C. amplivesiculatum from surveys conducted in the southwestern Gulf of California based on monthly sampling from June 1999 to August 2000. Surveys were conducted at 2 sites in the San Lorenzo Channel at depths of 8 - 12 m. The parameters measured were percent cover, biomass and structure in relation to size of the thalli. From each thallus the following measurements were taken: length and diameter, proportion of each thallus with reproductive structures and the degree of fragmentation (measured as number of small thalli). Mean biomass and cover varied seasonally with the maximum (1036.6 gm$^{-2}$ and 100% cover) present in June 2000 and the minimum in winter (0 gm$^{-2}$ and 0% cover). The longest fronds (3 m) occurred during July 1999 and August 2000, while the maximum diameters of 1.0 cm were present during October and November 1999. There were no significant linear relationships between length and diameter of the fronds, suggesting independent growth. Reproductive structures were common in June and July 1999 with monoic and dioic thalli present. Most fronds were monoic, and this is the first record of this feature for this species. Fragmentation occurred in September, and may be associated with stress from early gametangial reproduction and the combination of high temperatures and low nutrients. A hypothetical life cycle for the species is presented.

• 한국가축번식학회지
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• 제16권3호
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• pp.247-260
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• 1992

This study was carried out to investigate the histomorphological changes of ovaries obtained from 100 of 1-year-old female Korean loach(misgurnus anguillicaudatus). The light microscopic and ultrastructural changes ofooplasm and follicular membranes of oocytes, were observed by lightand transmission electron microscope during the reproductive cycle. All data were collected from November in 1991 to May in 1992. The results obtained in this study were as follows: The size of the nucleoli and number of the yolk granules increased as the oocytes grown. Yolk granules were loosely deposited in the oocytes as crystalline granules. Due to the presence of large early and late maturing oocytes, their ovaries were enlarged, transparent, granular and yellowish in color. The lattice was broken down at hydration, leaving the egg transparent. As the percentages of fish in LMO and RO stage increased from March to April, mean gonadosomatic index(GSI) values(18.49%) increased. Zona radiata change a squamous into cuboid shape in EMO stage. Processes from the granulosa cells and from the oocyte, microvilli grow and make contact with other in the pore canals of the zona radiata during vitellogenesis, but are withdrawn as the zona radiata becomes more compact and devoid of pore canals during oocyte maturation. Seasonal changes in the microscopic appearance of the ovaries were well correlated with those in both GSI and macroscopic appearance.

• 한국발생생물학회지:발생과생식
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• 제17권3호
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• pp.247-255
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• 2013

The gonadsomatic index (GSI) of mottled skate was the highest in April, GSI and HSI showed a reverse phase for its reproductive cycle. The fish had one pair of egg capsules, having 1 to 7 fertilized eggs, and spawned all the year round. When surveying the reproductive characteristics of females over 63 cm in disc width, we found the spawning peak was between April to June, and the appearance ratio of egg capsules was the highest in May (32.1%). The eggs were hatched at $8^{\circ}C$, $13^{\circ}C$, $18^{\circ}C$, water temperature (12.8 to $24.2^{\circ}C$), and the best hatching temperature was $18^{\circ}C$. The number of fish hatched was 4 to 5 fish/egg capsules, and the hatching rate was 100%. The sex ratios of hatching larvae were 45.5% female and 54.5% male. Therefore this study will provide fundamental data and information for artificial reproduction of the mottled skate.

• 한국과학교육학회지
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• 제23권6호
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• pp.617-626
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• 2003

과학교육에 있어 지금까지 개발된 수업모형은 마치 과학의 모든 목적을 달성시켜줄 것 같지만 실제 교육현장에 적용해 보면 기대하던 효과를 얻지 못하는 경우가 많다. 수업의 효과를 극대화하기 위해서는, 어느 한가지 방법만을 고수하는 것보다 개발된 수업모형을 유연성 있게 활용 할 수 있는 교사의 수업전략이 필요하다. 본 연구는 개념도와 순환학습을 통합한 수업이 개념도를 활용한 수업이나 순환학습과 비교하여 고등학생들의 학업성취도 및 과학적 탐구능력, 과학에 대한 태도에 어떠한 영향을 주는지 알아보는데 그 목적이 있다. 연구대상은 고등학교 2학년 여학생 324명으로, 개념도-순환학습 집단, 개념도 활용 집단, 순환학습 집단, 전통적 수업 집단의 4집단으로 나누어 수업하였다. 연구 단원은 고등학교 과학의 '생식과 유전' 단원으로 약 10주간 10차시에 걸쳐 수업처치 하였다. 수업처치 전, 후에 학업성취도 검사와 과학적 탐구능력, 과학에 대한 태도 검사를 실시하였고, 연구결과는 공변량 분석 (ANCOVA과)과 사후 검증(Duncan test)으로 분석하였다. 개념도-순환학습은 학생들의 과학 학업성취도를 향상시키는데 전통적인 수업보다 효과적이었고(p< .05) 그 효과면에서 개념도를 활용한 수업보다 떨어지지 않았다. 성취 수준이 상위와 중위 학생들은 수업방법에 따라 학업성취도에 차이를 보이지 않았으나, 하위학생들은 개념도를 활용한 수업이 가장 효과적이었다(p< .05). 개념도-순환학습은 학생들의 과학적 탐구능력을 신장시키는데 개념도를 활용한 수업이나 전통적 수업보다 효과적이었으며(p< .05) 그 효과 면에서 순환학에 떨어지지 않았다. 성취수준이 상위인 학생은 수업방식에 따라 과학적 탐구능력에 차이를 보이지 않았으나, 개념도-순환 학습은 중위와 하위 학생들의 과학탐구능력 향상에 순환학습 만큼 효과적이었다. 개념도-순환학습은 학생들의 과학에 대한 태도를 향상시키는데 전통적 수업보다 효과적이었으며(p< .05) 그 효과 면에서 개념도 활용 학습이나 순환학습에 뒤지지 않았다. 따라서 개념도 순환 학습은 모든 학생들의 수준을 고려 할 때 학생들의 학업성취도를 향상시키고, 과학적 탐구능력을 신장시키는데 개념도 활용학습이나 순환학습을 단독으로 적용하는 것보다 긍정적이라고 할 수 있다.

• 한국발생생물학회지:발생과생식
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• 제27권3호
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• pp.101-115
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• 2023

Environmental factors impact oyster growth, condition, and gonadal development, which is linked to gamete characteristics observed through histology. The reproductive cycle of bivalves is related to energy storage and utilization. Therefore, in this study, the year-round growth change and gonadal development of oysters were observed using histological analysis, and the biochemical composition changes were confirmed. The oysters used in this study are being nurtured in Gadeok-do, and 40 oysters were randomly sampled monthly from March 2021 to February 2022. Result of histological analysis of gonads, oysters were showed early development from December to February, late development from March and April, mature and ripe from May to July, spawned from August to October, and spent from November to December. Condition index values of oysters decreased in summer and autumn and increased again when entered the spent after spawning. The protein content of oysters was high in May, the maturity period, and the lipid content decreased during the spawning period. In addition, EPA and DHA, the major fatty acids of oysters, were low during the spawning period and high during the maturation period. As a result, this study suggested a close relationship between changes in oyster growth, biochemical composition, and the reproductive cycle.