• Journal of Life Science
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• v.12 no.4
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• pp.464-468
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• 2002

Enzymatic activities and fluorescence spectroscopic properties of the double mutant proteins P96L/F139W, P96L/F258W and a triple mutant protein P96L/F139W/F258W of tryptophan synthase $\alpha$ subunit from Escherichia coli was examined to study tertiary and local structure changes around the tryptophan residues. The enzymatic activities of P96l./F139W and P96L/F258W were similar, but P96L/F139W/F258W had lower activity, as compared to wild type. The fluorescence intensities of double mutant, P96L/F139W and P96L/F258W, were decreased but that of a triple mutant, P96L/F139W/F258W, was increased when compared to wild type. The sum of the maximum fluorescence intensity (fluorescence intensity at the λ$_{max}$) for the double mutant proteins was not equal to the intensity seen in the triple mutant protein. The enzymatic activity and fluorescence data indicate that the replacement of Pro$^{96}$ longrightarrowLeu might affect on the stability of helix 8 and the loop located between strand 4 and helix4. The result suggests that the tertiary structure of triple mutant (P96L/F139W/F258W), being different from wild type, might have more compact residual structure at the vicinity of 139 and 258.8.

• Journal of the Korean Society of Tobacco Science
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• v.21 no.1
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• pp.95-101
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• 1999

This study was carried out to analyze chemical components in burley tobacco using near infrared spectroscopy(NIRS). Samples were collected in '96 and '97 crop year. Calibration equations were developed by modified partial least square. The standard error performance(SEP) of '96 crop year samples between NIRS and standard laboratory analysis were 0.25% for nicotine, 0.18% for total nitrogen, 0.59% for crude ash, 0.32% for ether extracts, and 0.14% for chlorine, respectively. The analytical results of '97 crop year samples were similar to those of '96 crop year samples. The analytical result of '97 crop year samples analyzed by '96 calibration equation was more inaccurate than that of '96 crop year samples. The SEP of '96 or '97 crop year samples applying calibration equation derived from '96 plus '97 crop year samples was similar to that of '96 or '97 crop year samples analyzed by '96 or '97 calibration equation, respectively. The SEP of '97 crop year samples analyzed by calibration equation derived from '96 plus '97 crop year samples was more accurate than that of '97 crop year samples analyzed by '96 calibration equation. To improve the analytical inaccuracy caused by the difference of crop year between calibration and prediction samples, we need to include the prediction sample spectra which were different from calibration sample spectra in recalibration sample spectra, and then develop recalibration equation. The NIRS can apply to analyze burley leaf tobacco, leaf process or tobacco manufacturing process which were required the rapid analytical result.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.9
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• pp.1833-1839
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• 2011

In this study, Global Gravitational Model EGM2008, EGM96 and 16,786 gravity points measured on land were compared and analyzed. On the assumption that land gravity data is most accurate, the correlation coefficient, differences, MSE and difference variance along the height were computed between land gravity data and EGM2008, EG96. The correlation coefficient, land gravity data and EGM2008, was computed most largely with 0.824 and the correlation coefficient with EGM96 was computed with 0.538. The standard deviation of differences between land gravity and EGM2008, EGM96 were 13.196 magl, 18.685 mgal respectively. Also the difference variance scope of EGM2008 was smaller than EGM96. There was no large variance of free-air anomaly differences between land gravity data and EGM2008 along the height however free-air anomaly differences with EGM96 varied along the height changes. Consequently EGM2008 nicely expresses Korea gravity field more than EGM96.

• The Transactions of the Korea Information Processing Society
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• v.7 no.5
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• pp.1559-1569
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• 2000

CHILL96 is recommended as development language for telecommunication systems by ITU-T. In this paper, we describe the design and implementation of CHILL96 compiler using C++ intermediate code. Translation rules from CHILL96 to C++ are designed for code generation. The CHILL96 compiler is composed of four parts such s syntax analyzer, visibility checker, semantic analyzer and code generator, and each part has very close relationship with symbol table and abstract syntax tree. Performance evaluation has been performed for feasibility study. After performance evaluation, we conclude the CHILL96 compiler using C++ intermediate sho good performance compared with other CHILL compilers. In addition to high performance and portability, the CHILL96 compiler using C++ intermediate code helps application developers to maintain and enhance telecommunications software by translating CHILL96 program to C++ program.

• BMB Reports
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• v.31 no.6
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• pp.595-599
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• 1998

To investigate conformational information of a low oxygen affinity recombinant hemoglobin (rHb) containing $96Val{\rightarrow}Trp$ mutation at the ${\alpha}96$ position, we ave produced rHb (${\alpha}96Val{\rightarrow}Phe$) and rHb (${\alpha}96Val{\rightarrow}Tyr$), using the Escherichia coli expression system and site-directed mutagenesis. The oxygen affinity of rHb (${\alpha}96Val{\rightarrow}Phe$) is similar to that of human normal adult hemoglobin (Hb A). However, the oxygen affinity of rHb (${\alpha}96Val{\rightarrow}Tyr$) showed much lower oxygen affinity than Hb A which is similar to that of rHb (${\alpha}96Val{\rightarrow}Tyr$), providing an opportunity as a potential candidate for a hemoglobin-based blood substitute. Both rHb (${\alpha}96Val{\rightarrow}Phe$) and rHb (${\alpha}96Val{\rightarrow}Tyr)$ showed high cooperativity in oxygen binding. IH-NMR spectroscopy shows that both rHb (${\alpha}96Val{\rightarrow}Phe$) and rHb (${\alpha}96Val{\rightarrow}Tyr$) have very similar tertiary structure around the heme pockets and uaternary structure in the ${\alpha}_1/{\beta}_2$ subunit interface ompared to Hb A. The low oxygen affinity of rHb (${\alpha}96Val{\rightarrow}Tyr$) has been suggested to be due to a hydrogen bond caused by an extra hydroxyl group not present in rHb (${\alpha}96Val{\rightarrow}Phe$). However, investigation of the carbonmonoxy form of rHb (${\alpha}96Val{\rightarrow}Phe$) and (${\alpha}96Val{\rightarrow}Try$) in the presence of inositol hexaphosphate at low temperature suggests that low oxygen affinity of (${\alpha}96Val{\rightarrow}Try$) may arise from a mechanism different to that of rHb (${\alpha}96Val{\rightarrow}Trp$).

• Molecules and Cells
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• v.20 no.2
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• pp.173-182
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• 2005

Heat shock protein gp96 is an endoplasmic reticulum chaperone, belonging to the HSP90 family. The function of gp96 as a molecular chaperone was discovered more than 10 years ago, but its importance has been overshadowed by the brilliance of its role in immune responses. It is now clear that gp96 is instrumental in the initiation of both the innate and adaptive immunity. Recently, the roles of gp96 in protein homeostasis, as well as in cell differentiation and development, are beginning to draw more attention due to rapid development in the structural study of HSP90 and some surprising new discoveries from genetic studies of gp96. In this review, we focus on the aspect of gp96 as an ER molecular chaperone in protein maturation, peptide binding and the regulation of its activity.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.29 no.1
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• pp.53-60
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• 2016

This study discusses the thermal insulation capacity of variant of NO96 LNG (liquefied natural gas) cargo containment insulation system. Changing the insulation materials and the insulation layers of conventional GTT NO96 containment system, The thermal resistance and BOR(boil off rate) caused by the heat transfer between cryogenic and environmental temperature is discussed. Therefore, thermal analysis of LNG CCS(cargo containment system) is carried out to determine the insulation capabilities. Also, BOR is evaluated in terms of the total amount of heat invaded into CCS(cargo containment system). Variant of NO96 CCS such as NO96, NO96GW and NO96L3 membrane type during laden voyage is selected for the comparative study. Finite element model for heat transfer analysis is conducted by employing the equivalent thermal resistance model to simplify the complex insulation layers. Finally the results for each variant model are relatively compared and discussed to minimize the BOR.

• Restorative Dentistry and Endodontics
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• v.40 no.1
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• pp.96-96
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• 2015

• Journal of KIISE:Computing Practices and Letters
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• v.6 no.3
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• pp.329-338
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• 2000

CHILL language has been used for the software development of electronic switching and telecommunications system. ETRI CHILL-96 language is an extended CHILL language with the notions of object-orientation, concurrency, and generic type. In this paper, we discuss some design and implementation issues of ETRI CHILL-96 compiler. Renaming translation rules and function restructuring techniques are adapted for the purpose of preventing name conflict and producing debugging information. Those new extended features are preprocessed in the compilation and translated to extended CHILL intermediate codes. Such compilation technique enables ETRI CHILL-96 language to hold compatibility with software developed by CHILL language.

• Pediatric Infection and Vaccine
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• v.22 no.2
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• pp.55-62
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• 2015

Purpose: Varicella Zoster Immune Globulin (VZIG) is available in Korea for post-exposure prophylaxis of the Varicella-zoster virus (VZV) in high-risk patients. In July 2013, the United States Centers for Disease Control and Prevention (US CDC) recommended extending the time for administration of VariZIG$^{(R)}$ from within 96 hours up to 10 days after VZV exposure. This study was performed to analyze the effectiveness of VZIG prophylaxis between the two groups of patients who received VZIG within 96 hours and more than 96 hours of exposure to varicella. Methods: A retrospective chart review was performed in pediatric patients who received VZIG at Samsung Medical Center, Seoul, Korea from January 2001 to December 2012. Results: A total of 91 patients were identified. Fifty-seven patients were male (62.6%) and the median age was 5.91 years. Thirty-nine patients (42.9%) were exposed to VZV in the hospital. Underlying diseases were solid tumors (41.8%), hematologic malignancies (40.7%), and others (17.5%). Forty-five patients (49.5%) were hematopoietic cell transplant recipients. Seventy-four patients (81.3%) received VZIG within 96 hours after VZV exposure. There was no significant difference in the development of chickenpox between the two groups (2.7% vs. 5.9%, P=0.4664). In 22 seronegative patients, we also observed no significant difference between the groups in terms of the development of chickenpox (6.6% vs. 0%, P=0.667). Conclusions: This study showed that the effectiveness of VZIG for the prevention of chickenpox was comparable between patients who received VZIG within 96 hours and those who received VZIG more than 96 hours after exposure to VZV.