• Molecules and Cells
• /
• 제23권3호
• /
• pp.391-397
• /
• 2007

FAM92A1 (named FAM92A1-271) belongs to the family of proteins with conserved DUF1208 domains. Its function remains elusive. We identified two novel transcript variants (FAM92A1-251, FAM92A1-289) of FAM92A1. The presence of these transcripts in cancerous and normal cells, as well as their influence on cell prolifera-tion and apoptosis, were investigated. The subcellular location of FAM92A1 was determined by fluorescence microscopy. We found that FAM92A1-271 and FAM92A1-289 were highly expressed in both normal and cancerous cells, but FAM92A1-251 was only expressed at a mo-derate level in both types of cell. Overexpression of FAM92A1-271, FAM92A1-251 and FAM92A1-289 inhibited cell proliferation, caused S-phase arrest and induced apoptosis. Subcellular localization showed that FAM92A1 localizes to the nucleus. Our results show that FAM92A1 has different splicing variants, and that it may take part in regulating cell proliferation and apoptosis.

• International Journal of Oral Biology
• /
• 제33권2호
• /
• pp.45-50
• /
• 2008

Treponema denticola is the best studied oral spirochete and numerous studies have shown that it is strongly associated with periodontitis and expresses several putative virulence factors. In this study, we report on a surface protein of T. denticola, Td92, which is homologous to Tp92 of Treponema pallidum, an agent of syphilis. Immunofluorescence assay and immunogold labeling with anti-Td92 Ab revealed that Td92 had surface-exposed epitopes. And Td92 was capable of binding to fibronectin and KB cells, an oral epithelial cell line. In addition, Td92 could enter the KB cells. These results indicate that Td92 is a fibronectin-binding protein which can bind to and internalize into the host cells, facilitating the virulence of T. denticola.

• 천문학회보
• /
• 제36권2호
• /
• pp.143.2-143.2
• /
• 2011

보현산천문대 1.8 m 망원경과 2K CCD를 이용하여 2002년 4월과 2003년 5월에 중원소 함량이 아주 적은 구상성단 M53(NGC 5024)과 M92(NGC 6341)에 대하여 BVI CCD 측광관측을 수행하였다. 구상성단 M53과 M92의 정밀한 상대 나이 측정을 위하여 M53의 측광관측 자료에 대해 조동환과 이상각이 2007년 출간한 구상성단 M15(NGC 7078)와 M92를 대상으로 수행한 측광연구 논문에서 M92의 측광관측 자료를 분석할 때 적용한 꼭 같은 방식으로 전처리, PSF 측광, 표준화 등의 자료 분석을 수행하였다. 그리고 구상성단 M53의 V 대 BV, V 대 V-I, 그리고 V 대 B-I 색-등급도를 제시하였다. 구상성단 M53과 M92의 상대 나 이는 ${\Delta}$(B-V) 방법을 이용하여 도출하였다. 구상성단 M53과 M92 사이의 상대 나이 비교에서 M92의 절대 나이를 14 Gyr로 취할 경우 M53의 상대 나이가 M92의 상대 나이보다 $1.1-2.6{\pm}0.9$ Gyr 적은 것으로 유도하였다. 구상성단 M53과 M92의 이 상대 나이 차이는 M53과 M92의 약간 다른 수평계열 형태 차이를 유발했을 것으로 추정한다.

• Journal of Acupuncture Research
• /
• 제33권2호
• /
• pp.1-9
• /
• 2016

Objectives : I investigated whether snake venom can synergistically strengthen the cytotoxic effects of NK-92 cells, and enhance the inhibition of the growth of lung cancer cells including NCI-H358 through the induction of death receptor dependent extrinsic apoptosis. Methods : Snake venom toxin inhibited cell growth of NCI-H358 Cells and exerted non influence on NK-92 cell viability. Moreover, when they were co-cultured with NK cells and concomitantly treated with $4{\mu}g/m{\ell}$ of snake venom toxin, more influence was exerted on the inhibition of growth of NCI-H358 cells than BV or NK cell co-culture alone. Results : The expression of Fas, TNFR2 and DR3 and in NCI-H358 lung cancer cells was significantly increased by co-culture of NK-92 cells and treatment of $4{\mu}g/m{\ell}$ of snake venom toxin, compared to co-culture of NK-92 cells alone. Coincidentally, Bax, caspase-3 and caspase-8 - expressions of pro-apoptotic proteins in the extrinsic apoptosis pathway, demonstrated significant increase. However, in anti-apoptotic NF-${\kappa}B$ activities, activity of the signal molecule was significantly decreased by co-culture of NK-92 cells and treatment of $4{\mu}g/m{\ell}$ of snake venom toxin, compared to co-culture of NK-92 cells or snake venom toxin treated by NCIH358 alone. Meanwhile, in terms of NO generation, there is a significant increase, in co-culture of NK-92 cells with NCI-H358 cells as well as the co-culture of NK-92 cells and concomitant treatment of $4{\mu}g/m{\ell}$ of snake venom toxin. However, no synergistic increase of NO generation was shown in co-culture of NK-92 cells and treatment of $4{\mu}g/m{\ell}$ of snake venom toxin, compared to co-culture of NK-92 cells with NCI-H358 cells. Conclusion : Consequently, this data provides that snake venom toxin could be useful candidate compounds to suppress lung cancer growth along with the cytotoxic effect of NK-92 cells through extrinsic apoptosis.

• Nuclear Engineering and Technology
• /
• 제56권10호
• /
• pp.4115-4126
• /
• 2024

The swelling behavior of ferritic/martensitic steels (FC92-B/-N, HT9, and Gr.92) was investigated following 3.5 MeV Fe⁺⁺ ion irradiation. Tested alloys were helium-pre-implanted up to the peak contents of 120 and 240 appm with He/dpa ratio of 1 appm/dpa at room temperature and then exposed to self-ion beam to the peak damage conditions of 120 and 240 dpa at 475 ℃. Field-emission transmission electron microscopy was used to characterize the cavity evolution. FC92-B exhibited the highest resistance to swelling among the irradiated alloys. The final volumetric swelling of FC92-B reached 1.3 % at 70 dpa and 2.9 % at 140 dpa. On the other hand, HT9 exhibited the highest swelling, reaching 7.4 % at 140 dpa. Comparing the present swelling results at 140 dpa/140 appm He with swelling data at 280 dpa/280 appm He from our previous study, it was observed that Gr.92 and FC92-N swelled more at 140 dpa/140 appm He than at 280 dpa/280 appm He. This negative correlation between swelling and dose in Gr.92 and FC92-N is primarily attributed to the helium-associated swelling suppression at higher helium concentration of 280 appm. A bimodal cavity size distribution appeared only in Gr.92 and FC92-N at 280 dpa/280 appm. This result demonstrates that the excess amount of helium over 200 appm promoted early-stabilization of new-born cavities, resulting in preferentially enhanced cavity nucleation, while impeding the growth of nucleated cavities. An inhibition in cavity growth possibly led to an extended duration of nucleation-dominant stages, finally suppressing swelling in ion-irradiated Gr.92 and FC92-N alloys.

• Parasites, Hosts and Diseases
• /
• 제54권4호
• /
• pp.385-391
• /
• 2016

The discovery and understanding of antigenic proteins are essential for development of a vaccine against malaria. In Plasmodium falciparum, Pf92 have been characterized as a merozoite surface protein, and this protein is expressed at the late schizont stage, but no study of Pv92, the orthologue of Pf92 in P. vivax, has been reported. Thus, the protein structure of Pv92 was analyzed, and the gene sequence was aligned with that of other Plasmodium spp. using bioinformatics tools. The recombinant Pv92 protein was expressed and purified using bacterial expression system and used for immunization of mice to gain the polyclonal antibody and for evaluation of antigenicity by protein array. Also, the antibody against Pv92 was used for subcellular analysis by immunofluorescence assay. The Pv92 protein has a signal peptide and a sexual stage s48/45 domain, and the cysteine residues at the N-terminal of Pv92 were completely conserved. The N-terminal of Pv92 was successfully expressed as soluble form using a bacterial expression system. The antibody raised against Pv92 recognized the parasites and completely merged with PvMSP1-19, indicating that Pv92 was localized on the merozoite surface. Evaluation of the human humoral immune response to Pv92 indicated moderate antigenicity, with 65% sensitivity and 95% specificity by protein array. Taken together, the merozoite surface localization and antigenicity of Pv92 implicate that it might be involved in attachment and invasion of a merozoite to a new host cell or immune evasion during invasion process.

• 천문학회지
• /
• 제49권5호
• /
• pp.175-192
• /
• 2016

CCD photometric observations of the globular cluster (GC), M53 (NGC 5024), are performed using the 1.8 m telescope at the Bohyunsan Optical Astronomy Observatory in Korea on the same nights (2002 April and 2003 May) as the observations of the GC M92 (NGC 6341) reported by Cho and Lee using the same instrumental setup. The data for M53 is reduced using the same method as used for M92 by Cho and Lee, including preprocessing, point-spread function fitting photometry, and standardization etc. Therefore, M53 and M92 are on the same photometric system defined by Landolt, and the photometry of M53 and M92 is tied together as closely as possible. After complete photometric reduction, the V versus B − V , V versus V − I, and V versus B − I color-magnitude diagrams (CMDs) of M53 are produced to derive the relative ages of M53 and M92 and derive the various characteristics of its CMDs in future analysis. From the present analysis, the relative ages of M53 and M92 are derived using the Δ(B − V ) method reported by VandenBerg et al. The relative age of M53 is found to be 1.6 ± 0.85 Gyr younger than that of M92 if the absolute age of M92 is taken to be 14 Gyr. This relative age difference between M53 and M92 causes slight differences in the horizontal-branch morphology of these two GCs.

• 국제 전기통신 표준화 소식
• /
• 통권23호
• /
• pp.107-130
• /
• 1992

• The Korean Journal of Pain
• /
• 제27권1호
• /
• pp.92-92
• /
• 2014

• 컴퓨터교육학회논문지
• /
• 제5권3호
• /
• pp.79-87
• /
• 2002

알고리즘을 컴퓨터로 구현하여 수식 입력에 대한 출력을 과정없이 보여주는 프로그램이 컴퓨터 대수 시스템이다. 이 시스템에 연역적 추론을 자동화하는 과정을 수행하는 자동 연역 시스템을 결합하는 시도로 구현된 것이 Theorema 시스템이다. 오스트리아의 RISC 연구소에서 개발된 이 시스템은 Mathematica의 rewriting 기법을 사용하여 구현되었는데, 계산과 문제 해결, 증명을 할 수 있는 모드를 지원하고 있다. 본 논문에서는 계산 모드의 Theorema 언어를 구사하여 TI-92 그래픽 계산기에 탑재된 Derive 시스템의 일부를 시뮬레이션한 결과를 토대로 TI-92 메뉴얼을 Mathematica notebook의 형태로 생성하는 도구를 개발한 내용을 소대한다. 또한, 이렇게 하여 생성된 매뉴얼이 자바 서브릿을 통하여 웹기반 학습용 인터페이스로 연계되는 일련의 과정을 제시한다.