• Molecules and Cells
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• v.23 no.3
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• pp.391-397
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• 2007

FAM92A1 (named FAM92A1-271) belongs to the family of proteins with conserved DUF1208 domains. Its function remains elusive. We identified two novel transcript variants (FAM92A1-251, FAM92A1-289) of FAM92A1. The presence of these transcripts in cancerous and normal cells, as well as their influence on cell prolifera-tion and apoptosis, were investigated. The subcellular location of FAM92A1 was determined by fluorescence microscopy. We found that FAM92A1-271 and FAM92A1-289 were highly expressed in both normal and cancerous cells, but FAM92A1-251 was only expressed at a mo-derate level in both types of cell. Overexpression of FAM92A1-271, FAM92A1-251 and FAM92A1-289 inhibited cell proliferation, caused S-phase arrest and induced apoptosis. Subcellular localization showed that FAM92A1 localizes to the nucleus. Our results show that FAM92A1 has different splicing variants, and that it may take part in regulating cell proliferation and apoptosis.

• International Journal of Oral Biology
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• v.33 no.2
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• pp.45-50
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• 2008

Treponema denticola is the best studied oral spirochete and numerous studies have shown that it is strongly associated with periodontitis and expresses several putative virulence factors. In this study, we report on a surface protein of T. denticola, Td92, which is homologous to Tp92 of Treponema pallidum, an agent of syphilis. Immunofluorescence assay and immunogold labeling with anti-Td92 Ab revealed that Td92 had surface-exposed epitopes. And Td92 was capable of binding to fibronectin and KB cells, an oral epithelial cell line. In addition, Td92 could enter the KB cells. These results indicate that Td92 is a fibronectin-binding protein which can bind to and internalize into the host cells, facilitating the virulence of T. denticola.

• The Bulletin of The Korean Astronomical Society
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• v.36 no.2
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• pp.143.2-143.2
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• 2011

보현산천문대 1.8 m 망원경과 2K CCD를 이용하여 2002년 4월과 2003년 5월에 중원소 함량이 아주 적은 구상성단 M53(NGC 5024)과 M92(NGC 6341)에 대하여 BVI CCD 측광관측을 수행하였다. 구상성단 M53과 M92의 정밀한 상대 나이 측정을 위하여 M53의 측광관측 자료에 대해 조동환과 이상각이 2007년 출간한 구상성단 M15(NGC 7078)와 M92를 대상으로 수행한 측광연구 논문에서 M92의 측광관측 자료를 분석할 때 적용한 꼭 같은 방식으로 전처리, PSF 측광, 표준화 등의 자료 분석을 수행하였다. 그리고 구상성단 M53의 V 대 BV, V 대 V-I, 그리고 V 대 B-I 색-등급도를 제시하였다. 구상성단 M53과 M92의 상대 나 이는 ${\Delta}$(B-V) 방법을 이용하여 도출하였다. 구상성단 M53과 M92 사이의 상대 나이 비교에서 M92의 절대 나이를 14 Gyr로 취할 경우 M53의 상대 나이가 M92의 상대 나이보다 $1.1-2.6{\pm}0.9$ Gyr 적은 것으로 유도하였다. 구상성단 M53과 M92의 이 상대 나이 차이는 M53과 M92의 약간 다른 수평계열 형태 차이를 유발했을 것으로 추정한다.

• Journal of Acupuncture Research
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• v.33 no.2
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• pp.1-9
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• 2016

Objectives : I investigated whether snake venom can synergistically strengthen the cytotoxic effects of NK-92 cells, and enhance the inhibition of the growth of lung cancer cells including NCI-H358 through the induction of death receptor dependent extrinsic apoptosis. Methods : Snake venom toxin inhibited cell growth of NCI-H358 Cells and exerted non influence on NK-92 cell viability. Moreover, when they were co-cultured with NK cells and concomitantly treated with $4{\mu}g/m{\ell}$ of snake venom toxin, more influence was exerted on the inhibition of growth of NCI-H358 cells than BV or NK cell co-culture alone. Results : The expression of Fas, TNFR2 and DR3 and in NCI-H358 lung cancer cells was significantly increased by co-culture of NK-92 cells and treatment of $4{\mu}g/m{\ell}$ of snake venom toxin, compared to co-culture of NK-92 cells alone. Coincidentally, Bax, caspase-3 and caspase-8 - expressions of pro-apoptotic proteins in the extrinsic apoptosis pathway, demonstrated significant increase. However, in anti-apoptotic NF-${\kappa}B$ activities, activity of the signal molecule was significantly decreased by co-culture of NK-92 cells and treatment of $4{\mu}g/m{\ell}$ of snake venom toxin, compared to co-culture of NK-92 cells or snake venom toxin treated by NCIH358 alone. Meanwhile, in terms of NO generation, there is a significant increase, in co-culture of NK-92 cells with NCI-H358 cells as well as the co-culture of NK-92 cells and concomitant treatment of $4{\mu}g/m{\ell}$ of snake venom toxin. However, no synergistic increase of NO generation was shown in co-culture of NK-92 cells and treatment of $4{\mu}g/m{\ell}$ of snake venom toxin, compared to co-culture of NK-92 cells with NCI-H358 cells. Conclusion : Consequently, this data provides that snake venom toxin could be useful candidate compounds to suppress lung cancer growth along with the cytotoxic effect of NK-92 cells through extrinsic apoptosis.

• Parasites, Hosts and Diseases
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• v.54 no.4
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• pp.385-391
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• 2016

The discovery and understanding of antigenic proteins are essential for development of a vaccine against malaria. In Plasmodium falciparum, Pf92 have been characterized as a merozoite surface protein, and this protein is expressed at the late schizont stage, but no study of Pv92, the orthologue of Pf92 in P. vivax, has been reported. Thus, the protein structure of Pv92 was analyzed, and the gene sequence was aligned with that of other Plasmodium spp. using bioinformatics tools. The recombinant Pv92 protein was expressed and purified using bacterial expression system and used for immunization of mice to gain the polyclonal antibody and for evaluation of antigenicity by protein array. Also, the antibody against Pv92 was used for subcellular analysis by immunofluorescence assay. The Pv92 protein has a signal peptide and a sexual stage s48/45 domain, and the cysteine residues at the N-terminal of Pv92 were completely conserved. The N-terminal of Pv92 was successfully expressed as soluble form using a bacterial expression system. The antibody raised against Pv92 recognized the parasites and completely merged with PvMSP1-19, indicating that Pv92 was localized on the merozoite surface. Evaluation of the human humoral immune response to Pv92 indicated moderate antigenicity, with 65% sensitivity and 95% specificity by protein array. Taken together, the merozoite surface localization and antigenicity of Pv92 implicate that it might be involved in attachment and invasion of a merozoite to a new host cell or immune evasion during invasion process.

• Journal of The Korean Astronomical Society
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• v.49 no.5
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• pp.175-192
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• 2016

CCD photometric observations of the globular cluster (GC), M53 (NGC 5024), are performed using the 1.8 m telescope at the Bohyunsan Optical Astronomy Observatory in Korea on the same nights (2002 April and 2003 May) as the observations of the GC M92 (NGC 6341) reported by Cho and Lee using the same instrumental setup. The data for M53 is reduced using the same method as used for M92 by Cho and Lee, including preprocessing, point-spread function fitting photometry, and standardization etc. Therefore, M53 and M92 are on the same photometric system defined by Landolt, and the photometry of M53 and M92 is tied together as closely as possible. After complete photometric reduction, the V versus B − V , V versus V − I, and V versus B − I color-magnitude diagrams (CMDs) of M53 are produced to derive the relative ages of M53 and M92 and derive the various characteristics of its CMDs in future analysis. From the present analysis, the relative ages of M53 and M92 are derived using the Δ(B − V ) method reported by VandenBerg et al. The relative age of M53 is found to be 1.6 ± 0.85 Gyr younger than that of M92 if the absolute age of M92 is taken to be 14 Gyr. This relative age difference between M53 and M92 causes slight differences in the horizontal-branch morphology of these two GCs.

• Standardization Trend in Telecommunications
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• s.23
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• pp.107-130
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• 1992

• The Korean Journal of Pain
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• v.27 no.1
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• pp.92-92
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• 2014

• The Journal of Korean Association of Computer Education
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• v.5 no.3
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• pp.79-87
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• 2002

Theorema system is built on top of Mathematica to automate deductive reasoning processes as the combination of computer algebra system and deductive system. Theorema system that was developed at RISC in Austria provides three modes based on rewriting techniques; computation, problem solving and proving. This paper shows how the computation mode of Theorema can simulate Derive part for TI-92 graphic calculators to produce TI-92 manuals. Moreover, it also describes how such manuals can be converted to web-based learning materials via Java servlet.

• Annual Conference on Human and Language Technology
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• 1992.10a
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• pp.567-575
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• 1992

한글 문자인식 시스템을 개발하기 앞서 생각해야 할 것이 인식실험에 사용될 문자 데이타를 수집하는 것이다. 이 논문에서는 연구 개발자들에게 문자인식 실험에 필요한 충분한 데이타를 제공하며 필기체 문자 데이타를 표준화하여 문자인식 시스템 상호간의 성능을 객관적으로 평가하기 위하여 한글 필기체 문자 데이터베이스 PE92를 개발하였다. 여기서는 PE92 데이타베이스의 소개로서 먼저 PE92를 수집하는데 있어 고려사항들, 즉 필기자, 수집문자의 수, 수집용지의 규격, 데이타베이스의 저장, 데이타의 압축에 대하여 알아본다. 다음 PE92 데이타베이스의 규격을 알아본다.