• Korean Journal of Microbiology
• /
• v.35 no.3
• /
• pp.197-204
• /
• 1999

A total of 22 Leptospiua inlermgans field isolates from the ~ a t s captured in 5 provinces of Korea in 1996, and 6 antigenically closely related relerence serovars of lai, yeonchon, birkini. gem, mwogolo. and canicola were analysed. When the antigenic characteristics were analysed by reactivity with 7 monoclonal antibodies prepared with sh.ains belongng to serogroup Icterohaemorrhagiae. all 22 isolates showed the same reaction pattern with that of serovar lai. Large restriction fragment patterns obtained after cleavage of geno~nic DNAs with infrequently cuttimg restriction enzymes were analyzed by pulsed-field pel electrophoresis(PFGE). Identification of leptospira strains by PFGE with Nor I, Asc I or Iise I digests correlated with their antigenically typed serovars, silh a few exceptions. PFGE of isolates, except for JR89, digested wjth Nor I showed identical pattern w~th serovar lai, showing 13 Cragments between 940 kb and 63 kb. When PFGE pallerns of JR89 were compared with those of serovar lai, Not I digest showed additional two hands of 1000 kb and 460 kb, while Asc I digest showed 650 kb fragment and Fse I digest did not show the fragment of 280 kb. Whereas serovar yeonchon. which was isolated in Korea and identified as a new serovar previously. could be differentiated from serovar lai in antigenic reactivities with monoclonal antibodres. it showed the similar PFGE pattern with serovar lai includin~ reference and field isolates. It was suggested that Korean leptospiral field isolates are closely related in DNA level.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.30 no.2
• /
• pp.350-356
• /
• 2001

The purpose of this study is to verify the relation among vegetarian diet, physical activity and the risk factors of cardiovascular disease. Two hundred and forty five buddhist nuns (age : 23~79 yrs) were selected as vegetarians. For control subjects, 235 healthy female adults (age : 23~79 yrs) were selected. Study period was from October 1996 to February 1997. The contents are consist of food consumption survey, anthropometric measurement, amount of energy expenditure, physical activity and clinical examination. Results were summarized as follows : The average ages of vegetarians were $44.2{\pm}17.3\;yrs$ and those of non-vegetarians were $40.5{\pm}18.4\;yrs$. Average body mass indices (BMI) of vegetarians and non-vegetarians were $22.5{\pm}2.7\;and\;21.1{\pm}2.5$, WHRs were $0.9{\pm}0.1\;and\;0.8{\pm}0.1$, and the average duration of vegetarian diet of the vegetarians was 13.1{\pm}12.9 years. All the nutrient intakes of both groups were over RDA's except calcium and vitamin A intakes of non-vegetarians that were below RDA's Fiber and vitamin C intakes of the vegetarians were significantly higher than those of non-vegetarians (p<0.01). Average energy intakes of vegetarians and non-vegetarians were $2177.1{\pm}420.6\;kcal/day\;and\;2103.2{\pm}534.9\;kcal/day$, total daily energy expenditure were $1789.9{\pm}325.8\;kcal/day\;and\;1703.8{\pm}344.8\;kcal/day$, those by physical activity were $507.8{\pm}360.9\;kcal/day\;and\;400.0{\pm}247.8\;kcal/day$. Serum lipid level, blood sugar, systolic blood pressure of vegetarians were significantly lower than those of non-vegetarians, but ratio of HDL-cholesterol/total-cholesterol was higher than those of non-vegetarians. Levels of total serum cholesterol of vegetarians and non-vegetarians were 161.4{\pm}33.3 mg/dL and 189.6{\pm}33.6mg/dL, HDL-cholesterol were $48.2{\pm}11.4\;mg/dL\;and\;50.8{\pm}12.2\;mg/dL$, LDL-cholesterol were $86.6{\pm}26.9\;mg/dL\;and\;111.1{\pm}17.0\;mg/dL$, atherogenic indice(AI) were $2.4{\pm}0.6\;and\;2.9{\pm}0.7$, fasting blood sugar were $90.8{\pm}14.3\;mg/dL\;and\;103.6{\pm}24.6\;mg/dL$, systolic blood pressure were $107.5{\pm}14.9\;mmHg\;and\;119.3{\pm}15.3\;mmHg$. Energy expenditure by physical activity was negatively correlated with levels of serum total-cholesterol and LDL-cholesterol and atherogenic indice(AI). Therefore, physical activity and vegetarian diet effectively helped reduce blood lipids related to cardiovascular disease.

• Korean Journal of Environmental Biology
• /
• v.28 no.3
• /
• pp.133-142
• /
• 2010

This study was performed to investigate the community structure of the macrobenthic invertebrates around Ui Island, Sinan-gun, Jeollanam-do, Korea. Benthic invertebrates were collected during May and September 2008 at seven stations. A total of 63 macrobenthic species was collected. The overall average macrobenthos density and biomass were 268 inds. $m^{-2}$ and 11.54 gWWt $m^{-2}$, respectively. Based on abundance data, there were 5 dominant species accounting for approximately 72.00% of total individuals. The polychaetes Notomastus latericeus, Scolelepis sagittaria, Amaeana occidentalis, Glycera chirori and the amphipoda Mandibulophoxus mai were found in high densities. The conventional multi-variate statistics (cluster analysis and non-metric multidimensional scaling) applied to assess spatial variation in macrobenthic assemblages. As a result, three communities could be distinguished: a sand dominated, a mixed sediment and a third community in the mud dominated station. The community in the sand dominated station was characterized by high abundance of the polychaetes S. sagittaria and the amphipods M. mai, Monoculodes sp. and Grandifoxus malipoensis. However, the most common species within the mixed sediment station were the polychaetes N. latericeus, A. occidentalis and amphipoda Urothoe sp.. Finally the mud dominated station was numerically dominated by the polychaetes Scolelepis sp., Heteromastus filiformis and Sigambra tentaculata. In conclusion, the sediment composition may be important factors controlling of the macrobenthic community structure in the study area.

• Korean Journal of Organic Agriculture
• /
• v.20 no.4
• /
• pp.715-725
• /
• 2012

This study was performed to make the method of food waste manufacturing for feeding to earthworm and different ratio of earthworm cast was investigated for applying vegetable plants that cultivated in a family or roof garden. We made conventional treatment of gardening soil and prepared soils with earthworm cast (the only fertilizer) at 30, 50, 70 and 100% for planting vegetable plants and then we studied its growth feature. The treatment was repeated three times with 12 weeks for each time. The cultivation of radish and chinese cabbage were managed at the same cultivation level for RDA standard cultivation method, and each sample of soils and plants was analyzed by appropriate analysis method. The growth investigation was performed 12 week after planting, and as for growth characteristics, height, leaf diameter, leaf number, leaf length, fresh weight and dry weight were investigated. The chemical characteristics, soils and plants, were surveyed. As for the growth characteristics investigated, radish and chinese cabbage showed the biggest growth in 70% and 50% treatment with average growth of all characteristics, height, leaf diameter, leaf number, leaf length, fresh weight and dry weight, respectively. Applying these results, a new equation for vermicast recommendation for radish and Chinese cabbage in summer (June-August) was presented. The earthworm cast 50% level with the gardening soil showed the high production in chinese cabbage. In case of radish, earthworm cast 70% with gardening soil level showed great growth, significantly.

• The Korean Journal of Nuclear Medicine Technology
• /
• v.15 no.1
• /
• pp.113-116
• /
• 2011

Purpose: Acetylcholine receptor antibodies cause acetylcholine receptor loss, which is responsible for failure of the neuromuscular junction in the acetylcholine receptor autoantibody. The disease characterized by muscle weakness and fatigue, myasthenia gravis(MG) occurs when the body inappropriately produces antibodies against acetylcholine receptors, and thus inhibits proper acetylcholine signal transmission. And this reason, the measurement of acetylcholine receptor antibodies can be of considerable value in disease diagnosis. Methods: From 2010. August to September, we tested orderd AchRAb 19 samples to get the results. 1. Pipette $5{\mu}{\ell}$ undiluted patient sera and kit control and add 125I AChR $50{\mu}{\ell}$ and incubate at R.T for 2 hours. 2. Pipette $50{\mu}{\ell}$ of anti-human IgG into each tube, and incubate at $2{\sim}8^{\circ}C$ for 2 hours. 3. Pipette $25{\mu}{\ell}$ precipitation enhancer into each tube and add 1mL washing solution into all tubes. 4. Centrifuge each tube for 20minutes at $2{\sim}8^{\circ}C$ at 1500g. 5. Aspirate or decant the supernatant. 6. Pipette 1 mL washing solution into all tubes and resuspend the pellet and repeat centrifugation. 7. Aspirate or decant the supernatant and count all tubes on a gamma counter. Results: Cut off value is 0.2 nmol/L and the results taken below 0.2 nmol/L are negative, the results above that identified as being positive values. We assayed the 19 patients samples and got 7 positive results. Of which, 6 patients were diagnosed as MG.(85.7%). Conclusions: Acetylcholine Receptor autoantibody test is intended for use by persons only for the quantitative determination of it in human serum. Even if measurement of the antibodies is not a routine test, it can be of considerable value in disease diagnosis.

• Korean Journal of Ecology and Environment
• /
• v.51 no.1
• /
• pp.71-85
• /
• 2018

A recent topic of interest within ecology is ecosystem services that are importantly connected with biodiversity. Biodiversity is a fundamental component of ecological studies and has been the subject of many studies worldwide. However, studies on the diversity and distribution of freshwater fish were not undertaken in earnest until 2000, and the number of internationally available studies is therefore still limited. In this study, we used data from "The Survey and Evaluation of Aquatic Ecosystem Health (hereafter SEAEH)" within the framework of which whole water systems on the Korean peninsula are annually monitored to investigate and determine the current status of the diversity and distribution of freshwater fish, as well as to elucidate major factors impacting freshwater fish. A total of 130 species from 28 families were identified at around 953 sites in the SEAEH investigation of 2014. The species Zacco platypus (relative abundance: 28.2%) and Z. koreanus (19.3%) were identified as the most abundant species on the Korean peninsula, and besides these 20 endangered species, 51 endemic species and 4 exotic species were also collected. Highest (96 species) and lowest (72 species) diversity were identified in the Han River Watershed and the Yeongsan/Seomjin River Watershed, respectively. The mean endemic rate was 32.7%, which is higher than the previous rate determined in 1995. The exotic species Micropterus salmoides, Lepomis macrochirus and Carassius cuvieri were found in the whole river system except north Yeongdong, whereas Oreochromis niloticus occurred only at three sites due to the cold water temperatures in winter. Totally, 28 species were found to be translocated to different water systems not connected with known original habitats, most translocations occurring in the Nakdong River and the Yeongdong region. Among the translocated species, Opsariichthys uncirostris amurensis, and Erythroculter erythropterus were dominantly distributed in the mid- to lower reaches of Nakdong River where they may be harmful to the native fish assemblages due to their strong predation. The construction of weirs in connection with the "4 River Project" generated changes in fish assemblages before, during and after the project, resulting in altered habitat condition. Especially, a decrease in the number of endemic species and an increase in the abundance of lentic species were seen. Human-induced global warming will allow exotic species requiring high water temperatures to adapt to the environmental conditions of the Korean peninsula, which may enhance the diversity of native fishes and create changes in distribution. For the establishment of realistic and efficient management and protection strategies for Korean freshwater fish, SEAEH, which produces fundamental and quantitative data for Korean streams, is an important and necessary tool.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.22 no.3
• /
• pp.183-193
• /
• 2020

This study was conducted to evaluate the effect of environment factors on the growth of autumn season cultivation of Kimchi cabbage using the big data in terms of public open data(weather, soil information, and growth of crop, etc.). The growth data and the environment data such as temperature, daylength, and rainfall from 2010 to 2019 were collected. As a result of composing the correlation matrix, the height and leaf number showed high correlation in growing degree days(GDDs) and daylength, and the yield showed negative correlation in growing degree days and the concentration of clay. GDDs and daylength explained about 89% and 84% of variation in height, respectively. These two environmental factors also explained about 85% and 79% of variation in leaf numbers, respectively. In contrast, the coefficient of determination was low for yield when GDDs and concentration of clay was used. The outcome of regional statistical analysis indicated that relationship between yield and sum of sand and silt were high in Haenam and Jindo areas. Hierarchical cluster analysis, which was performed to verify the association of yield, GDDs, and concentration of clay, showed that Haenam and Jindo were clustered together. Although GDDs and yield vary by year and region, and there are regions with similar concentration of clays, observation data are grouped as the result. These suggests that GDDs and soil texture are expected to be related to yield. The cluster analysis results can be used for further data analysis and agricultural policy establishment.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.27 no.2
• /
• pp.85-92
• /
• 2007

This study was carried out to select the promising forage crops fer forage production in paddy field of YARI, RDA, Milyang from 1999 to 2001. The species of forage crop used in this experiment were barley(5 varieties), wheat(2), rye(3) and Italian ryegrass(IRG, 3). Stages of heading, milk and yellow ripe of barley were $20{\sim}24$ April, $6{\sim}10$ May and $21{\sim}24$ May, respectively. In milk to yellow ripe stage, dry matter(DM) yield of barley was $7.89{\sim}9.66$ MT per ha, and averaged ADF and NDF contents were $26.9{\sim}33.3%\;and\;53.1{\sim}59.2%$, respectively. Albori and Naehanssalbori were the most promising varieties among the barley. The growth of wheat was a little late compared to that of barley, but it seemed to be crop having higher forage yield and nutritive value. In milk to yellow stage, DM yield of wheat was $9.13{\sim}10.38$ MT per ha, and nutritive value of wheat was very good(ADF $25.9{\sim}31.7%$ and NDF $53.3{\sim}55.8%$). Heading stage of rye was $20{\sim}24$ April, and it seemed to be suitable far harvest at heading to flowering stage because of heavy lodging after flowering. In flowering stage, DM yield of rye was 13.64 MT per ha, and nutritive value of rye was the lowest among 4 species(ADF $33.1{\sim}38.0%$ and NDF $56.4{\sim}65.0%$). Heading stage of early maturing IRG was 2 May and it seemed to be suitable for harvest at flowering stage of middle May in cropping system. In flowering stage, DM yield of early type IRG was 5.51 MT per ha, and averaged ADF and NDF contents were $30.1{\sim}34.7%\;and\;59.7{\sim}60.5%$, respectively. The results demonstrated that the promising forage crops far forage production in paddy field were rye, barley, early maturing IRG and wheat in Yeongnam region. In Southern region, rye harvesting at early May was recommended when considered middle May of rice transplanting period, and barley(Albori and Naehanssalbori) and early maturing IRG harvesting at middle May were recommended when considered late May of rice planting period. And also, wheat with high nutritive value and possibility for harvesting at late May were recommended in case of transplanting period at early June.

• Journal of Korean Home Economics Education Association
• /
• v.18 no.4 s.42
• /
• pp.85-102
• /
• 2006

The objectives of this study were to classify of life style of secondary school girl students, to investigate the relationships between life styles, attitude toward appearance and clothing attitude. The method of this study was survey research by using questionnaires. Subjects were 658(middle school students 327, high school students 331) girl students located in Jeollabukdo province. In this statistical analysis, SPSS 11.5 for Windows program was utilized to calculate frequency, mean, cronbach's ${\alpha}$, factor analysis, t-test, Pearson's correlation, multiple regression analysis. The results of this research were as follows: The results of analysing the factors to the response lifestyles, attitude toward appearance and clothing attitude emerged five dimensions(digital orientation, material orientation, positive activity, achievement orientation, frugality), three dimensions(needs conformity value toward appearance), five dimensions(fashion pursuit, gender attractiveness, self-expression, aesthetic, and modesty). High school girls' students showed higher digital orientation and positive activity life styles, attitude toward appearance, clothing attitude except of modesty. Clothing attitude variables except of modesty had positive correlations with lifestyles and attitude toward appearance. However, modesty of clothing had negative correlations with life styles and attitude toward appearance. As a conclusion, secondary school girl students' life styles and attitude toward appearance constituted important characteristics which could affect clothing attitude directly.

• Korean Journal of Environmental Agriculture
• /
• v.31 no.4
• /
• pp.318-327
• /
• 2012

BACKGROUND: For Non-Point Source(NPS) loads reduction, pollutant loads need to be quantified for major farming methods. The objective of this study was to evaluate impacts of farming methods on NPS pollutant loads from a paddy rice field during the growing season. METHODS AND RESULTS: The height of drainage outlet, amount of fertilizer, irrigation water quality were considered as farming factors for scenarios development. The control was derived from conventional farming methods and four different scenarios were developed based combination of farming factors. A field scale model, CREAMS-PADDY(Chemicals, Runoff, and Erosion from Agricultural Management Systems for PADDY), was used to calculate pollutant nutrient loads. The data collected from an experimental plot located downstream of the Idong reservoir were used for model calibration and validation. The simulation results agreed well with observed values during the calibration and validation periods. The calibrated model was used to evaluate farming scenarios in terms of NPS loads. Pollutant loads for T-N, T-P were reduced by 5~62%, 8~37% with increasing the height of drainage outlet from 100 mm of 100 mm, respectively. When amount of fertilizer was changed from standard to conventional, T-N, T-P pollutant loads were reduced by 0~22%, 0~24%. Irrigation water quality below water criteria IV of reservoir increased T-N of 9~65%, T-P of 9~47% in comparison with conventional. CONCLUSION(S): The results indicated that applying increased the height of drainage after midsummer drainage, standard fertilization level during non-rainy seasons, irrigation water quality below water criteria IV of reservoir were effective farming methods to reduce NPS pollutant loads from paddy in Korea.