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Enzymatic Saccharification of Salix viminalis cv. Q683 Biomass for Bioethanol Production

  • Kim, Hak-Gon;Song, Hyun-Jin;Jeong, Mi-Jin;Sim, Seon-Jeong;Park, Dong-Jin;Yang, Jae-Kyung;Yoo, Seok-Bong;Yeo, Jin-Ki;Karigar, Chandrakant S.;Choi, Myung-Suk
    • Journal of Forest and Environmental Science
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    • v.27 no.3
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    • pp.143-149
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    • 2011
  • The possibility of employing biomass of Salix viminalis cv. Q683 as a resource of bio-energy was evaluated. The chemical analysis of S. viminalis cv. Q683 leaf biomass showed components such as, extractives (2.57%), lignin (39.06%), hemicellulose (21.61%), and cellulose (37.83%), whereas, its stem was composed of extractives (1.67%), lignin (23.54%), hemicellulose (33.64%), and cellulose (42.03%). The biomass of S. viminalis cv. Q683 was saccharified using two enzymes celluclast and viscozyme. The saccharification of S. viminalis cv. Q683 biomass was influenced by enzymes and their strengths. The optimal enzyme combination was found to be celluclast (59 FPU/g substrate) and viscozyme (24 FBG/g substrate). On saccharification the glucose from leaf and stem biomass was 7.5g/L and 11.7g/L, respectively after 72 hr of enzyme treatment. The biomass and enzyme-treated biomass served as the feedstock for ethanol production by fermentation. The ethanol production from stem and leaf biomass was 5.8 g/L and 2.2 g/L respectively, while the fermentation of the enzymatic hydrolysates yielded 5 g/L to 8 g/L bioethanol in 72 hours.

Synthesis of Copolyimide Containing 5,15-Bis(mesityl)-10,20-bis(4-amino)porphyrin (5,15-Bis(mesityl)-10,20-bis(4-amino)porphyrin을 포함하는 Copolyimide의 합성)

  • Kim, Hooi-Sung;Lee, Min-Ho;Kim, Chun-Ho;Kim, Mi-Ra;Lee, Jin-Kook
    • Applied Chemistry for Engineering
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    • v.10 no.6
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    • pp.828-831
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    • 1999
  • 5,15-Bis(mesityl)-10,20-bis(4-amino)porphyrin was synthesized with meso-(mesityl)dipyrromethane and 4-nitrobenzaldehyde. It was characterized with $^1H$-NMR spectroscopy, UV spectrophotometer, and GC-MASS. In UV spectrum, Soret-band(438 nm) and Q-bands(526, 572, 611 nm) were confirmed. In NMR spectrum, the characteristic peak of porphyrin was detected at -2.65 ppm. Copolyimide containing porphyrin structure was synthesized with mesityl-$TPP(NH_2)$, 3,3',4,4'-benzophenonetetracarboxylic dianhydride(BTDA) and 2,2-bis(4-(4-aminophenoxy)phenyl) hexafluoropropane(BAPHF) and characterized with UV and FT-IR spectrophotometer. The $T_g$ and $T_m$ of the synthesized copolyimide were 220.7 and $369.2^{\circ}C$, respectively.

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An Analysis on Shipment Structure of House Tangerine in Jeju (하우스감귤의 출하구조 분석)

  • Ko, Seong-Bo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.7
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    • pp.555-561
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    • 2019
  • This study analyzed the shipbuilding structure of House Tangerine over a ten day period and according to the month in Jeju. By estimating and analyzing the price flexibility function of House Tangerine, the aim was to determine if the shipment control can stabilize and increase the incomes of House Tangerine farmers and derive policy implications. The greatest decline in the coefficients of the ten-day price flexibility occurred in the equations from early June to late July. Therefore, the shipment control of House Tangerine is required more during early June and late July. The coefficient of DUM_Q, indicating the year in which the quality of House Tangerine is somewhat deteriorated, was statistically significant and had a (-) sign. The coefficients of DUM_SUK, indicating the year in which Chuseok (Korean Thanksgiving Day) was in October, was statistically significant and had a (-) sign in the middle and late October. The greatest decline in the coefficient of monthly price flexibility function occurred in July, June, and September. Therefore, shipment control is required more in July, June, and September. The (-) signs of the coefficients of DUM_Q and DUM_SUK suggest that the need for shipment control is more important when the quality is less than better quality and that the necessity of shipment control is required more when Chuseok is in October, respectively.

Importance of FISH combined with Morphology, Immunophenotype and Cytogenetic Analysis of Childhood/Adult Acute Lymphoblastic Leukemia in Omani Patients

  • Goud, Tadakal Mallana;Al Salmani, Kamla Khalfan;Al Harasi, Salma Mohammed;Al Musalhi, Muhanna;Wasifuddin, Shah Mohammed;Rajab, Anna
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.16
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    • pp.7343-7350
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    • 2015
  • Genetic changes associated with acute lymphoblastic leukemia (ALL) provide very important diagnostic and prognostic information with a direct impact on patient management. Detection of chromosome abnormalities by conventional cytogenetics combined with fluorescence in situ hybridization (FISH) play a very significant role in assessing risk stratification. Identification of specific chromosome abnormalities has led to the recognition of genetic subgroups based on reciprocal translocations, deletions and modal number in B or T-cell ALL. In the last twelve years 102 newly diagnosed childhood/adult ALL bone marrow samples were analysed for chromosomal abnormalities with conventional G-banding, and FISH (selected cases) using specific probes in our hospital. G-banded karyotype analysis found clonal numerical and/or structural chromosomal aberrations in 74.2% of cases. Patients with pseudodiploidy represented the most frequent group (38.7%) followed by high hyperdiploidy group (12.9%), low hyperdiploidy group (9.7%), hypodiploidy (<46) group (9.7%) and high hypertriploidy group (3.2%). The highest observed numerical chromosomal alteration was high hyperdiploidy (12.9%) with abnormal karyotypes while abnormal 12p (7.5%) was the highest observed structural abnormality followed by t(12;21)(p13.3;q22) resulting in ETV6/RUNX1 fusion (5.4%) and t(9;22)(q34.1;q11.2) resulting in BCR/ABL1 fusion (4.3%). Interestingly, we identified 16 cases with rare and complex structural aberrations. Application of the FISH technique produced major improvements in the sensitivity and accuracy of cytogenetic analysis with ALL patients. In conclusion it confirmed heterogeneity of ALL by identifying various recurrent chromosomal aberrations along with non-specific rearrangements and their association with specific immunophenotypes. This study pool is representative of paediatric/adult ALL patients in Oman.

Factors Affecting the Survival of Frozen Thawed Bovine In Vitro Produced Blastocysts

  • Gustafsson, H.;Larsson, B.;Shamsuddin, M.;Jaakma, U.;Emanuelson, U.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.1
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    • pp.7-12
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    • 2001
  • Factors Affecting the Survival of Frozen Thawed Bovine In Vitro Produced Blastocysts. The effect of some factors on the post-thaw survival of a total of 240 in vitro produced bovine blastocysts was investigated using logistic regression analysis. The explanatory variables tested were: type of culture medium before freezing (TCM 199 supplemented with BSA, BSAITS (BSA+insulin+transferrin+selenium), ECS (estrous cow serum) with or without BOEC (bovine oviductal epithelial cells), age of the blastocyst (Day 7, Day 8+9), morphological appearance before freezing (distinct=Q1 or indistinct=Q2 inner cell mass) and type of cryoprotectant (glycerol, 1.0 M or ethylene glycol, 1.6 M). The survival after thawing based on the post-thaw quality and the development after co-culture with BOEC for 24 and 48 hours. Day 7 blastocysts had an almost three times better chance of survival than Day 8+9 blastocysts. Q1, Day 8+9 blastocysts had higher odds to survive after 48 hours in culture than Q2 blastocysts (p<0.05). Blastocysts produced in BSAITS medium had the best chances of survival; however, the odds were not always significant. Blastocysts frozen in glycerol had a better post-thaw quality rating than those frozen in ethylene glycol; however, the difference in post-thaw development at culture was not significant. The relationship between post-thaw quality and post-thaw development at culture was significant (p<0.05). The developmental stage and/or age of the embryo and culture medium where development up to blastocyst takes place affect the post-thaw survival of the bovine embryos.

Carbon Stocks in Tree Biomass and Soils of Quercus acutissima, Q. mongolica, Q. serrata, and Q. variabilis stands (상수리나무, 신갈나무, 졸참나무, 굴참나무 임분의 임목 바이오매스와 토양 탄소 저장량)

  • Lee, Sang Tae;Chung, Sang Hoon;Kim, Choonsig
    • Journal of Korean Society of Forest Science
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    • v.111 no.3
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    • pp.365-373
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    • 2022
  • We compared carbon stocks in tree biomass and soils of Quercus acutissima, Q. mongolica, Q. serrata, and Q. variabilis stands. A total of 531 plots (Q. acutissima: 110 plots, Q. mongolica: 177 plots, Q. serrata: 96 plots, Q. variabilis: 148 plots) were examined between 2016 and 2021 to determine the tree biomass and soil carbon stocks throughout the country. The carbon stocks of tree biomass were significantly higher in Q. mongolica (mean stand age, 57 years, 144.9 Mg C ha-1) than in Q. variabilis (mean stand age, 43 years, 123.7 Mg C ha-1), Q. serrata (mean stand age, 43 years, 120.1 Mg C ha-1), and Q. acutissima (mean stand age, 36 years, 113.2 Mg C ha-1) stands. The soil carbon concentration was significantly higher in Q. mongolica (A: 43.1 mg C g-1) than in Q. serrata (31.0 mg C g-1), Q. variabilis (25.31 mg C g-1), and Q. acutissima (24.4 mg C g-1) stands. The soil carbon stocks were significantly higher in Q. mongolica (116.8 Mg C ha-1) than in Q. acutissima (49.3 Mg C ha-1) stands. Total carbon stocks of tree biomass and soil were highest in Q. mongolica (262 Mg C ha-1), followed by Q. serrata (218 Mg C ha-1), Q. variabilis (211 Mg C ha-1), and Q. acutissima (163 Mg C ha-1) stands. Multiple linear regressions were performed to estimate the total carbon stocks of the four Quercus spp., and results showed that total carbon stocks increased with increasing elevation, mean diameter at breast height, and basal areas. Basal area and elevation of Quercus spp. stands were important explanatory variables based on multiple linear regressions for estimating carbon stocks.

2DEG Transport Analysis in AlGaAs/GaAs Interface by MONTE-CARLO Method (MONTE-CARLO 방법에 의한 AlGaAs/GaAs 계면의 전자 전달특성 분석)

  • Nam, Seung-Hun;Jung, Hak-Ki;Kim, Bong-Ryul
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.26 no.2
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    • pp.94-101
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    • 1989
  • Transport properties of 2DEG at AlGaAs/GaAs interface such as average electron energy, flight distance, each valley occupancy ratio, average electron velocity for various fields are investigated by MONTE-CARLO method. As the electric field increases, more electrons transit drastically from (000) valley to (000) upper valley. This phenomenon shows the nonstationary effect such as velocity overshoot. The duration of the transient decreases from about 1.4 psec for electric field E = 7KV/cm to about 0.7 psec for 12KV/cm. The average electron velocity during transient transport in 2DEG is about 8 times the steady-state velocity for E = 12KV/cm at room temperature. In comparison with bulk GaAs the peak velocity in the 2DEG is higher than that in even pure bulk GaAs at electric field E = 7 KV/cm. On the basis of the fact that the electrons in the 2DEG have larger peak velocity and shorter transient time of velocity than those in the bulk GaAs, it is suggested that the device with 2DEG may obtain higher mobility than that with bulk GaAs.

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Cluster Analysis of 12 Chinese Native Chicken Populations Using Microsatellite Markers

  • Chen, G.H.;Wu, X.S.;Wang, D.Q.;Qin, J.;Wu, S.L.;Zhou, Q.L.;Xie, F.;Cheng, R.;Xu, Q.;Liu, B.;Zhang, X.Y.;Olowofeso, O.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.8
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    • pp.1047-1052
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    • 2004
  • The genomes of Chinese native chicken populations were screened using microsatellites as molecular markers. A total of, 528 individuals comprisede12 Chinese native chicken populations were typed for 7 microsatellite markers covering 5 linkage groups and genetic variations and genetic distances were also determined. In the 7 microsatellite loci, the number of alleles ranged from 2 to 7 per locus and the mean number of alleles was 4.6 per locus. By using fuzzy cluster, 12 Chinese native chicken populations were divided into three clusters. The first cluster comprised Taihe Silkies, Henan Game Chicken, Langshan Chicken, Dagu Chicken, Xiaoshan Chicken, Beijing Fatty Chicken and Luyuan Chicken. The second cluster included Chahua Chicken, Tibetan Chicken, Xianju Chicken and Baier Chicken. Gushi Chicken formed a separate cluster and demonstrated a long distance when comparing with other chicken populations.

Development of kNN QSAR Models for 3-Arylisoquinoline Antitumor Agents

  • Tropsha, Alexander;Golbraikh, Alexander;Cho, Won-Jea
    • Bulletin of the Korean Chemical Society
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    • v.32 no.7
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    • pp.2397-2404
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    • 2011
  • Variable selection k nearest neighbor QSAR modeling approach was applied to a data set of 80 3-arylisoquinolines exhibiting cytotoxicity against human lung tumor cell line (A-549). All compounds were characterized with molecular topology descriptors calculated with the MolconnZ program. Seven compounds were randomly selected from the original dataset and used as an external validation set. The remaining subset of 73 compounds was divided into multiple training (56 to 61 compounds) and test (17 to 12 compounds) sets using a chemical diversity sampling method developed in this group. Highly predictive models characterized by the leave-one out cross-validated $R^2$ ($q^2$) values greater than 0.8 for the training sets and $R^2$ values greater than 0.7 for the test sets have been obtained. The robustness of models was confirmed by the Y-randomization test: all models built using training sets with randomly shuffled activities were characterized by low $q^2{\leq}0.26$ and $R^2{\leq}0.22$ for training and test sets, respectively. Twelve best models (with the highest values of both $q^2$ and $R^2$) predicted the activities of the external validation set of seven compounds with $R^2$ ranging from 0.71 to 0.93.

Clonal Variation of Physical Characteristics and Mineral Composition in Acorn of Quercus acutissima and Q. serrata Seed Orchard

  • Kim, Chang-Soo;Kim, Du-Hyun;Han, Sang-Urk;Shim, Tae-Heum
    • Journal of agriculture & life science
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    • v.45 no.6
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    • pp.47-55
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    • 2011
  • This study investigated mineral element concentrations of acorns in Quercus acutissima and Quercus serrata seed orchard, so that to estimate the variation of these species based on the chemical composition in different clones from plus trees. The acorns were collected from ten clones of each species grown in the same clonal seed orchard. The nutritional concentration of acorns was significantly different between the clones and species. The concentration of nutrient for the whole acorn followed in this general sequence: P > K > Na > Mg > Ca > Mn > Fe > Zn > Cu. The mineral concentrations of acorns in clones of Q. acutissima and Q. serrata contained P (494 to 684 and 541 to 672 mg/100 g), K (114 to 569 and 140 to 251 mg/100 g), Na (57 to 121 and 49 to 85 mg/100 g), Mg (29 to 37 and 26 to 42 mg/100 g), Ca (10 to 53 and 26 to 68 mg/100 g), Mn (0.5 to 3.4 and 1.8 to 4.5 mg/100 g), Fe (0.7 to 1.1 and 0.0 to 2.2 mg/100 g), Zn (0.34 to 0.81 and 0.38 to 0.84 mg/100 g), and Cu (0.13 to 0.40 and 0.09 to 0.34 mg/100 g) respectively. Even though acorns of Q. serrata are smaller in size than Q. acutissima, acorns of Q. serrata contained significantly higher concentration of phosphorus, calcium, iron and manganese than Q. acutissima. Based on the mineral composition of the acorns, this study has shown that the clones of Q. acutissima and Q. serrata have different ability to accumulate mineral nutrients which could indicate the variation of Quercus species in terms of mineral acquisition and accumulation.