• 한국식품과학회지
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• 제39권2호
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• pp.128-132
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• 2007

감귤 착즙박을 주 성분으로 하는 버섯배양배지를 만들고 간, 표고, 새송이, 산호침, 참부채 및 영지 버섯 균을 접종하여 균사체 제품을 만들었다. 만들어진 균사체는 착즙박에 비하여 대체적으로 조회분은 증가하였으나, 총산, 환원당, 조단백질 및 조지방은 착즙박과 함량이 비슷하거나 일부 감소하는 경향을 보였다. 대부분의 버섯균사체 중 유기산의 함량은 착즙박의 함량과 비슷하거나 감소하는 경향을 보였으나 새송이 버섯에서는 착즙박의 2.7배 이상 유기산의 함량이 증가하였다. 버섯균사체 중 fructose는 9.20-11.06%로서 착즙박보다 증가하는 경향을 보였으나, 새송이 버섯에서는 2.65%로서 함량이 매우 감소하였으며, glucose의 경우는 모든 버섯 균사체에서 그 함량이 착즙박보다 매우 감소하는 경향을 보였는데, 산호침 버섯 및 참부채 버섯에서 감소하는 현상이 더욱 심하였다. 버섯균사체 중 sucrose는 착즙박의 함량보다 감소하는 경향을 보였는데, 간버섯과 새송이 버섯에서 감소현상이 심하였다. 버섯균사체의 narirutin 함량은 448.67-932.98 mg%로서 대부분 착즙박에 비하여 비슷하거나 약간 감소하는 경향을 보였으나, hesperidin의 함량은 3,019.94-4,980.94 mg%로서 간버섯을 제외하면 착즙박보다 17.81-52.61%증가하여 농축되는 현상을 나타내었다. 버섯균사체 중 TDF 함량은 38.87-51.49%, NDF 함량은 11.94-17.67%, ADF함량은 11.00-14.09%로서 대부분 착즙박의 함량과 비슷하거나 증가하는 현상을 보였다. 버섯균사체의 ${\beta}-glucan$ 함량은 9.43-13.69%로서 착즙박과 비슷하거나 조금 감소하는 경향을 보였다. 총 polyphenol은 모든 버섯 균사체에서 8.01-20.28 mg%로서 그 함량이 비슷하거나 대부분 감소하였다. 버섯균사체의 EDA 및 NSA는 새송이 버섯 균사체를 제외한 모든 버섯 균사체에서 착즙박보다 감소하는 경향을 보였다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권16호
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• pp.6871-6879
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• 2014

Background: The purpose of this study was to evaluate the potential association of five (p.P47S, p.R72P, PIN3 Ins16bp, p.R213R and r.13494g>a) polymorphisms of TP53 with the risk of developing breast cancer in North Indian Punjabi population. Methods: We screened DNA samples of 200 sporadic breast cancer patients (197 females and 3 males) and 200 unrelated healthy, gender and age matched individuals for the polymorphisms. Results: For the p.P47S polymorphism, we observed the PP genotype in 99.5% of the patients and PS genotype in only 1 patient. All the controls had the wild type PP genotype. The frequency of RR, RP and PP genotype of p.R72P was 23.5% vs 33.5%, 51.5% vs 45.5% and 25% vs 21% in patients and controls respectively. Heterozygous (RP) genotype was increased in breast cancer patients as compared to controls (51.5 vs 45.5%) and showed 1.61 fold significantly increased risk for breast cancer (OR=1.61, 95% CI, 1.01-2.58, p=0.04). In breast cancer patients the frequencies of A1A1, A1A2 and A2A2 genotypes of PIN3 Ins16bp polymorphism were 67%, 26% and 7% respectively whereas in controls the genotype frequencies were 68.5%, 27.5% and 4% respectively, with no significant difference. For p.R213R (c.639A>G), all individuals had homozygous wild type genotype. The frequencies of GG, GA and AA genotypes of TP53 r.13494g>a polymorphism were 62 vs 67.5%, 33 vs 28% and 5 vs 4.5% in patients and controls respectively, again without significant difference. We observed that RP-A1A1 genotype combination of p.R72P and PIN3 Ins16bp and RP-GG combination of p.R72P and r.13494g>a polymorphism showed significant risk of breast cancer (OR=1.65, 95%CI: 0.98-2.78, p=0.05; OR=1.72, 95%CI: 1.01-2.92, p=0.04). Conclusion: The results of present study indicated that among the five TP53 polymorphisms investigated, the p.R72P polymorphism, and the RP-A1A1 and RP-GG genotype combination contribute to breast cancer susceptibility in North Indians.

• 한국전기전자재료학회논문지
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• 제17권1호
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• pp.7-15
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• 2004

The ferroelectric SBT thin films as a material of capacitors for non-volatile FRAMs have some problems that its remanent polarization value is relatively low and the crystallization temperature is quite high abovc 80$0^{\circ}C$. Therefore, in this paper, SBTN solution with S $r_{0.9}$B $i_{2.1}$T $a_{1.8}$N $b_{0.2}$$O_{9}$ composition was synthesized by sol-gel method. Sr(O $C_2$ $H_{5}$)$_2$, Bi(TMHD)$_3$, Ta(O $C_2$ $H_{5}$)$_{5}$and Nb(O $C_2$ $H_{5}$)$_{5}$ were used as precursors, which were dissolved in 2-methoxyethanol. SBTN thin films with 200 nm thickness were deposited on Pt/Ti $O_2$/ $SiO_2$/Si substrates by spin-coating. UV-irradiation in a power of 200 W for 10 min and rapid thermal annealing in a 5-Torr-oxygen ambient at 76$0^{\circ}C$ for 60 sec were used to promote crystallization. The films were well crystallized and fine-grained after annealing at $650^{\circ}C$ in oxygen ambient. The electrical characteristics of 2Pr=11.94 $\mu$C/$\textrm{cm}^2$, Ps＋/Pr＋=0.54 at the applied voltage of 5 V were obtained for a 200-nm-thick SBTN films. This results show that 2Pr values of the UV irradiated and rapid thermal annealed SBTN thin films at the applied voltage of 5 V were about 57% higher than those of no additional processed SBTN thin films. thin films.lms.s.s.

• Asian Pacific Journal of Cancer Prevention
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• 제16권18호
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• pp.8533-8539
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• 2016

Background: B-cell chronic lymphocytic leukemia B (B-CLL), the most common type of leukemia, may be caused by apoptosis deficiency in the body. Adipose tissue-derived mesenchymal stem cells (AD-MSCs) as providers of pro-apoptotic molecules such as tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), can be considered as an effective anti-cancer therapy candidate. Therefore, in this study we assessed the role of tumor necrosis factor-producing mesenchymal stem cells oin apoptosis of B-CLL cells resistant to fludarabine-based chemotherapy. Materials and Methods: In this study, after isolation and culture of AD-MSCs, a lentiviral LeGO-iG2-TRAIL-GFP vector containing a gene producing the ligand pro-apoptotic with plasmid PsPAX2 and PMDG2 virus were transfected into cell-lines to generate T293HEK. Then, T293HEK cell supernatant containing the virus produced after 48 and 72 hours was collected, and these viruses were transduced to reprogram AD-MSCs. Apoptosis rates were separately studied in four groups: group 1, AD-MSCs-TRAIL; group 2, AD-MSCs-GFP; group 3, AD-MSCs; and group 4, CLL. Results: Observed apoptosis rates were: group 1, $42{\pm}1.04%$; group 2, $21{\pm}0.57%$; group 3, $19{\pm}2.6%$; and group 4, % $0.01{\pm}0.01$. The highest rate of apoptosis thus occurred ingroup 1 (transduced TRAIL encoding vector). In this group, the average medium-soluble TRAIL was 72.7pg/m and flow cytometry analysis showed a pro-apoptosis rate of $63{\pm}1.6%$, which was again higher than in other groups. Conclusions: In this study we have shown that tumor necrosis factor (TNF) secreted by AD-MSCs may play an effective role in inducing B-CLL cell apoptosis.

• 대한전자공학회논문지SD
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• 제45권2호
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• pp.118-124
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• 2008

디지털 비디오 방송표준(DVB-S2)은 순방향 에러 코딩방법으로 BCH와 LDPC을 연결한 시스템을 내부코딩으로 사용한다. DVB-S2에서 LDPC 코드는 11개의 서로 다른 부호화 율을 정의하고 있기 때문에, DVB-S2 LDPC 복호기는 다양한 부호화 율을 지원해야 한다. 11개의 부호화 율 중에서 7가지(3/5, 2/3, 3/4, 4/5, 5/6, 8/9, 9/10)는 균일한 부호화 율이고, 나머지 4가지(1/4, 1/3, 2/5, 1/2)는 비균일 부호화 율이다. 본 논문에서는 균일한 LDPC 코드를 위한 유연한 복호기를 제시한다. 제안된 복호기는 칩의 면적, 메모리의 효율, 처리속도 등에서 많은 장점을 갖는 반 병렬 복호 구조와 변수노드와 체크노드의 내부 연결선을 줄이고 다양한 부호화 율을 지원할 수 있도록 Benes 네트워크를 결합하여 블록크기가 64,800까지 사용가능하도록 설계하였다. 제안하는 복호기는 200MHz에서 193.2MbPs의 처리속도를 갖으며, 면적은 $16.261m^2$이고, 전력은 공급전압이 1.5V에서 198mW의 소모를 보인다.

• Radiation Oncology Journal
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• 제8권1호
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• pp.115-124
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• 1990

고 에너지 23MV광자선의 특성 중 임상적용에 중요한 심부선량 백분율, 조직-최대선량비 (TMR), 산란-최대선량비 (SMR), 표면선량 및 출력선량 보정계수등의 변수가 이온전리 (IC-10)함 및 평행 평판전리 (PS-033)함에 의해 측정 조사되었다. 명목상의 23 MV X-선에 대한 가속에너지는 $18.5\pm0.5$ MV로 측정되었다. Mevatron KD 8067의 23 MV X-선의 중심선속의 반가층이 기하학적인 좁은 선속으로 측정되었으며 반가층의 두께는 $24.5\;g/cm^2$이었다. 조직-최대선량비는 심부선량백분율표에서 구해졌으며, 실측치와 비교한 결과 각 조사면의 크기와 깊이에서 약간의 차이를 보였으나 평균 $0.7\pm0.5$의 오차를 나타내고 있어 계산에 의한 TMR 값과 잘 일치함을 보였다. 조사면 $0\times0\;cm^2$의 TMR 값은 zero 조사면의 유효감약계수에 의한 값과, 각 조사면의 조직-최대 선량비로 부터 비선형최소자승법에 의해 구해진 유효선흡수계수 및 반가층 측정에 의한 유효선흡수 계수에 의한 값들로 비교되었으며, $\mu=0.0283{\pm}0,0002cm^{-1}$을 보였고, 세 방법 모두 오차범위내에서 잘 일치됨을 보였다. 한편, 불규칙 조사면의 선량계산에 이용될 SMR은 조사면의 반경 50cm까지 계산되어 대형 조사 면에서도 선량율 산출이 이루어지도록 하였다. Mevatron KD 8067의 23 MV X-선의 조직 표면선량은 SSD 100 cm, 1$10\times10\;cm^2$의 조사면에서 최대조직선량율의 $9.6\%,\;25\times25\;cm^2$에서는 $25.4\%$를 보였다.

• 수산해양기술연구
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• 제29권3호
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• pp.200-213
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• 1993

A model experiment on a midwater rope trawl net which is used in the North Pacific to catch alaska pollack is carried out in the circulating tank to examine the basic efficiency of the net. The prototype is the net used by M/S Hanil(1, 179GT, 2, 700PS), a Korean trawler. The model net was made according to the Tauti's Similarity Law of Fishing Gear in 1/100 scale by considering the condition of the tank. To measure the basic efficiency of the standard model net, the vertical opening and width between some points marked on the net were measured, and the hydrodynamic resistance were determined. Then the constructive conditions of the net were varied as follows and the factors were measured again to compare the efficiency of those nets with that of the standard net(A-1 type) front weight multiplied 1.5 times: A-2 type. buoyancy and depressing force multiplied 1.7 times: A-3 type. front weight multiplied 1.5 times on A-3 type: A-4 type. depressors rigged at ground rope: B type. cod-end stuffed with cashmylon wad: C type. The results obtained can be summarized as follows: 1. The vertical opening at the center of head rope was steeply decreased with the flow velocity increasing and the vertical opening H(m) can be expressed in H=1.2v super(-1.2)(v : flow velocity in m/sec). The width of the net varied a little when the flow velocity was over 0.4m/sec, and the width of net mouth showed about 37% of the distance between the fore tips of net pendant. The shape of net mouth was almost a circle at 0.2m/sec and then steeply flatted elliptically with the flow velocity increasing and the area of mouth S(m super(2)) can be expressed in S=(1.65-2.3v)$\times$10 super(-2). The hydrodynamic resistance of the net increased almost linearly with the flow velocity increasing and the resistance R(kg) can be expressed in R=3.2$\times$d/l$\times$abv. where d/l denotes the mean of d(diameter of netting twine) and l(length of a leg in a mesh) from wing tip to the end of bag-net except cod-end on the side pannel, and a denotes the strectched circumference of the net at the fore end of a meshed part and b the stretched length of the whole net from wing tip to the end of cod-end. 2. In the condition-varied nets, the vertical opening of head rope showed some increase in every type net except the C type, and the increase showed the greatest in the B type by 30~54%, whereas it showed decrease in the C type by 5~10%. Variation of the area of net mouth showed almost the same tendency as the vertical opening and the increase showed the greatest in the B type by 20%, whereas it showed decrease in the C type by 12%. Hydrodynamic resistance showed some increase in every type compared with the standard net, and the rate of increase indicated 5~10% in the A-2, A-3 and A-4 type, 22% in the B type and 3% in the C type.

• Journal of Chest Surgery
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• 제24권4호
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• pp.404-420
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• 1991

Surgical treatment of congenital and acquired heart disease preceded the development of accurate techniques for diagnosis, heart lung machine and cardiopulmonary bypass, intraoperative myocardial protection, operative techniques and cardiac anesthesia. For 5 years from Sep. 1985 to Sep. 1990, six hundred cases of open heart surgeries [OHS] were performed in the department of Thoracic & Cardiovascular Surgery, Pusan Paik Hospital, College of Medicine, Inje University. The results were summarized as follows. 1. The annual number of OHS[cases per year] was above 100 since 1987, and the increasing rate of cases was 23.5% per year since 1986. 2. Among the total 600 cases, there were 470 cases of congenital heart diseases and 130 cases of acquired. Age range of the congenital patients was 8 months to 44 years with the mean age of 10 years, and acquired patients was 16 to 56 years with the mean age of 36 years. 3. Among the 470 congenital anomalies, there were 429 cases of acyanotic and 41 cyanotic patients. Totally, VSD was 286 cases[60.6%], ASD 103 cases[21.9%], TOF 35 cases [7.4%], PS 20 cases [4.1%], ECD 12 cases [2.0%], Ebstein`s anomaly 3 cases [0.6%], Valsalva sinus rupture 3 cases [0.6%] and others. The appropriate one stage radical operations were applied to the all congenital cases with the result of 2.6% immediate postoperative hospital mortality rate. 4. Among the 130 acquired cases, there were 122 cases of valvular heart diseases, 6 of heart tumors [5 myxoma, one malignant histiocytoma], one of LA thrombus and one of annuloaortic ectasia. Cardiac tumors and LA thrombus were removed through the atrial septal approach. Bentall procedure was adopted to the annuloaortic ectasia case. AVR, MVR and TVA [DeVega procedure] were applied to 120 valve diseases, and there were also one of OMC and one of MVA[Jerome-Kay procedure]. 5. Among the 120 valve replacement cases, there were 87 of single valve replacement cases [AVR: 8, MVR: 79], 11 of double valve replacement [AVR+MVR: 11], 12 of MVR+TVR and 10 of MVR+AVR+TVA. The total number of implanted prosthetic valves were 141. In MVR, 45 of St. Jude Medical valves, 63 of Carpentier-Edward valves and 4 of Ionescu-Shiley valves were used. In AVR, 18 of St. Jude Medical valves and 11 of Carpentier-Edward valves were used. in MVR, 29mm and 31mm sized valves were used mostly and In AVR, 23mm sized valves were used mostly. 6. Postoperatively many kinds of complications were occurred. Among them, wound problems [30 cases], low output syndrome [29 cases], arrhythmia [20 cases], pleural effusion and pneumothorax [13 cases] were occurred frequently. The postoperative immediate hospital mortality was 3.0% in total [congenital 2.6%, acquired 4.6%].

• KSBB Journal
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• 제31권1호
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• pp.27-32
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• 2016

Several CoA transferases from Clostridium beijerinckii, C. perfringens and Klebsiella pneumoniae were examined for biosynthesis of lactate-containing polyhydroxyalkanoates (PHAs) in recombinant Escherichia coli XL1-Blue strain. The CB3819 gene and the CB4543 gene from C. beijerinckii, the pct gene from C. perfringens and the pct gene from K. pneumoniae, which encodes putative CoA transferase gene, respectively, was co-expressed with the Pseudomonas sp. MBEL 6-19 phaC1437 gene encoding engineered Pseudomonas sp. MBEL 6-19 PHA synthase 1 ($PhaC1_{Ps6-19}$) to examine its activity for the construction of key metabolic pathway to produce poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)]. The recombinant E. coli XL1-Blue expressing the phaC1437 gene and CB3819 gene synthesized poly(3-hydroxybutyrate) [P(3HB)] homopolymer to the P(3HB) content of 60.5 wt% when it was cultured in a chemically defined medium containing 20 g/L of glucose and 2 g/L of sodium 3-hydroxybutyrate. Expression of the phaC1437 gene and CB4543 gene in recombinant E. coli XL1-Blue also produced P(3HB) homopolymer to the P(3HB) content of 51.2 wt% in the same culture condition. Expression of the phaC1437 gene and the K. pneumoniae pct gene in recombinant E. coli XL1-Blue could not result in the production of PHAs in the same culture condition. However, the recombinant E. coli XL1-Blue expressing the phaC1437 gene and the C. perfringens gene could produce poly(3-hydroxybutyrate-co-lactate [P(86.4mol%3HB-co-13.7 mol%LA) up to the PHA content of 10.6 wt% in the same culture condition. Newly examined CoA transfereases in this study may be useful for the construction of engineered E. coli strains to produce PHA containing novel monomer such lactate.

• 한국환경농학회지
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• 제37권2호
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• pp.73-78
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• 2018

2016년 한국에서 재배 및 유통되고 있는 뚱딴지 12점을 수집하여, 새만금간척지에 재배하였다. 간척지 적응성을 검토한 결과 다음과 같은 결론을 얻을 수 있었다. 1. 뚱딴지를 간척지에 재배하였을 때, 간척지 특성상 0.3% 이상일 때 밭작물 재배가 어려운데, 개화 전 8월 가뭄으로 인하여 토양 염농도가 전체적으로 0.3%이상 올라갔음에도 초장 및 수량으로 보았을 때 높은 염농도에 대한 적응성이 있는 것으로 판단된다. 2. 개화기 무렵 꽃이 50%이상 피었을 때 뚱딴지 초장을 조사한 결과 WY3와 WI1가 207 cm, 202 cm로 가장 컸으며 WG1, WH1, PS1 144 cm, 139 cm, 131 cm로 가장 작았다. 3. 11월 뚱딴지를 수확하여 수량을 확인한 결과 흰색 뚱딴지는 WG1이 549 kg/10 a, WH1이 477 kg/10 a로 가장 많은 수량을 나타내었으며, 자색 뚱딴지에서는 PY4와 PY1이 각각 10 a당 615 kg, 584 kg로 우수한 수량을 보였다. 4. 수집한 뚱딴지 12점의 이눌린 함량을 분석한 결과 PY1이 33.9 g/100 g으로 가장 많은 이눌린 함량을 보였으며, 다음으로 D2가 33.7 g/100 g으로 많았다 결론적으로 내염성이 우수한 뚱딴지가 간척지에서도 적응할 수 있는 것으로 판단되며, 수집 뚱딴지 중 흰색은 수량 및 이눌린함량을 고려하여 G1이 간척지에 적합할 것으로 보이며, 자색은 Y1이 이눌린함량이 두 번째로 많지만, 수량과의 관계를 고려할 때 간척지에서 재배하는데 더 적합한 것으로 판단된다. 각각 색깔별로 우수한 뚱딴지 2점을 가지고 내염성 및 간척지 재배법에 대한 연구가 더욱 필요할 것으로 보인다.