• Proceedings of the Korea Information Processing Society Conference
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• 2011.11a
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• pp.862-865
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• 2011

현재 IPv4 주소가 고갈됨으로써 IPv6 를 사용할 수 밖에 없는 상황에 놓여지게 되었다. 그리고 IPv6 주소체계를 이용하다 보니 이에 따른 보안취약점들이 발견되었다. IPv6 보안취약점에는 확장헤더, ICMPv6, NDP, 다양한주소에 따른 취약점들이 있다. 이것뿐만 아니라 IPv4 와 IPv6 네트워크 간에 통신이 가능한 6to4, ISATAP, Tunnel Broker, Teredo 와 같은 기술이 나오게 되었고, 이러한 것들 또한 보안취약점이 발견되었다. 6to4 보안취약점에는 분산반사 스푸핑 트래픽 공격이 있고, Teredo 에는 로컬 peer 발견 절차에 다른 캐쉬 오버플로우 공격이 있다. 그리고 ISATAP 에는 라우터를 가장한 MITM 공격이 있고, Tunnel Broker 에는 Tunnel Borker 와 Tunnel Server 를 위장하여 공격하는 방법이 있다. 이렇게 IPv6 주소체계로 바뀌면서 생기는 새로운 보안취약점들에 대응하기 위해 현재 존재하는 라우터 같은 네트워크장비들도 보안취약점에 대응할 수 있는 변화가 필요하다.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.9 no.6
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• pp.1760-1767
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• 2008

In this study, we analyzed the physical and chemical properties such as proximate analysis, ultimate analysis, heating values, and thermogravimetric analysis for the organic sludges discharged from an industrial complex in Ulsan. The average water, combustible, and ash content of organic sludges were 72.9, 18.5, and 8.6%, respectively. And according to the ultimate analysis of organic sludges, the C, O, H, N, and S compositions were 33.9, 26.4, 4.4, 4.4, and 0.6%, respectively. According to the results of investigating the lower heating values, 6 sludges were on the range of $1,500{\sim}2,000\;kcal/kg$ and 4 sludges were on the range of over 2,000 kcal/kg. Therefore, these 10 sludges could be directly applied to industries which try to use the energy by direct incineration.

• Nuclear Engineering and Technology
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• v.25 no.4
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• pp.561-569
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• 1993

DOT4.2-QAD-CG coupling method was used to analyze the dose rates outside the side and the bottom shield system of CANDU 6 plant. The average dose rates at the main airlock and the new fuel loading area are approximately 6 $\mu$Sv/h as it is required. The calculated dose rates have a good agreement with the measurements at the operating CANDU 6 plant. The method used in this paper can be applied to the radiation shielding analysis of Wolsong 2, 3, and 4 CANDU 6 type plants which will be constructed in the near future.

• Journal of Bio-Environment Control
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• v.4 no.2
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• pp.181-187
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• 1995

This study was conducted to investigate the effect of NH$_4$H$_2$PO$_4$ on pH of the nutrient solution using municipal tap water during hydroponic culture of crisp lettuce (Lactuca sativa var. capitata) seedlings. The composition of starter solution was different from that of supplementary solution. The pH in the nutrient solution was suddenly declined and recovered as the supplementary solution was supplied. The pH of nutrient solution was increased with high temperature and, on the contrary, the EC of nutrient solution was decreased. It shows that plant absorbed nutrients more than water in given solution when the temperature and light was high. After supplying supplementary solution in 1st and End experiment, pH was slowly increased to 7 in NH$_4$H$_2$PO$_4$ 0.25me/$\ell$, but maintained 6.4-6.5 in NH$_4$H$_2$PO$_4$ 3me/$\ell$ and 6me/$\ell$. In 3rd experiment, pH was slowly increased from 6.7 to 7.4 in NH$_4$H$_2$PO$_4$ 0.25me/$\ell$, but decreased from 6-6.5 to 5-5.5 in NH$_4$H$_2$PO$_4$ 3me/$\ell$ and 6me/$\ell$. So it is suggested that the concentration between 0.25 me/$\ell$ and 3 me/$\ell$ by concentration base or the amount of NH$_4$H$_2$PO$_4$ between 1me/6 $\ell$ and 7me/6 $\ell$ by total quantity in solution is appropriate for stabilizing pH in the nutrient solution. Also this experiment suggests that hand operated measurements must be cautious due to the change of pH and EC within a 24-hour cycle.

• KSBB Journal
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• v.32 no.3
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• pp.193-198
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• 2017

Sialylation is important in producing therapeutic proteins such as antibody, cytokine and fusion protein. Thus, enhancement of sialylation is usually performed in CHO cell cultures. ${\alpha}2,6$-Sialyltransferase (ST), which plays a key role in the attachment of ${\alpha}2,6-sialic$ acid, is present in human cells but not in Chinese hamster ovary (CHO) cells. Overexpression of ${\alpha}2,6-ST$ can be used for enhancing the degree of sialylation and achieving human-like glycosylation. In this study, we constructed CHO cells producing human cytotoxic T-lymphocyte antigen4-immunoglobulin (hCTLA4-Ig) as well as ${\alpha}2,6-ST$. Transfected CHO cells were selected using G418 and stable cell line was established. Profiles of viable cell density and hCTLA4-Ig titer in an overexpressed cell line were similar to those of a wild-type cell line. It was confirmed that the total amount of sialic acid was increased and ${\alpha}2,6-sialic$ acid was attached to the terminal residues of N-glycan of hCTLA4-Ig by ESI-LC-MS. Compared to 100% of ${\alpha}2,3-sialic$ acid in wild type cells, 70.9% of total sialylated N-glycans were composed of ${\alpha}2,6-sialic$ acid in transfected cells. In conclusion, overexpression of ${\alpha}2,6-ST$ in CHO cells led to the increase of both the amount of total sialylated N-glycan and the content of ${\alpha}2,6-sialic$ acid, which is more resemble to human-like structure of glycosylation.

• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.217-221
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• 2002

dTDP-D-glucose 4,6-dehydratase (TDPDH) catalyzes the conversion of dTDP-D-glucose to dTDP-4-keto-6-deoxy-D-glucose, and requires $NAD^＋$ as a coenzyme for its catalytic activity. The dTDP-D-glucose 4,6-dehydratase from Streptomyces antibioticus $Tu{\ddot}99$ tightly binds $NAD^＋$ [19]. In order to determine the role of lysine-148 in the $NAD^＋$ binding, the lysine of the dTDP-D-glucose 4,6-dehydratase from Streptomyces antibioticus $Tu{\ddot}99$ was mutated to various amino acids by site-directed mutagenesis. The catalytic activity of the four mutated enzymes of TDPDH did not recover after addition of $NAD^＋$ . However, the activity of K159A, the mutated enzyme of UDP-D-glucose 4-epimerase (UDPE), recovered after the addition of $NAD^＋$ [15]. Although dTDP-glucose 4,6-dehydratase, and UDP-galactose (glucose) 4-epimerase are members of the short-chain dehydrogenase/reductase SDR family and the lysine-148 of TDPDH was highly conserved as in UDPE (Lys-159), the function of the lysine-148 of TDPDH was different from that of UDPE. The mutated enzymes showed that the lysine-148 of the dTDP-D-glucose 4,6-dehydratase played no role in the $NAD^＋$ binding. Accordingly, it is suggested that the lysine-148 of the dTDP-D-glucose 4,6-dehydratase is involved in the folding of TDPDH.

• Journal of Convergence for Information Technology
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• v.8 no.5
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• pp.211-217
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• 2018

The purpose of this paper is to explore the possibility and strategic directions of Korean agriculture in the future by analyzing domestic and foreign successful cases leading to the 6th industrialization of agriculture by utilizing 4th Industrial Revolution technologies. To do this, we surveyed the key technologies of the 4th Industrial Revolution, and examined successful cases of the US, Korea and China. As results of these case studies, we recognized that 4th Industrial Revolution technologies could play important roles in the 6th industrialization of agriculture, and suggested meaningful strategic implications using ABCD model.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.4
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• pp.398-403
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• 1998

The objective of the present study was to examine the developmental rates, and the stage of development in relation to time since fertilization, of in vitro produced buffalo embryos. Buffalo cumulus-oocyte complexes obtained from slaughterhouse ovaries were matured and fertilized in vitro. The fertilized oocytes (n = 248) were then co-cultured with buffalo oviductal epithelial cells and evaluated for the developmental stages on Days 2, 4, 6, 7, 8, 9 and 10 post-insemination. The peak of 4-cell stage embryos was observed on Day 2 (63.7 %), whereas Day 4 was marked by peaks of 6-8-cell stage embryos (20.9%) and 16-cell stage embryos to early morulae (50%). On Days 6, 7, 8, 9, and 10 post-insemination, 49.5, 48.3, 38.3, 33.8 and 33.4% embryos were found to be at morula/compact morula stages, 8.8, 12.5, 25.4, 6.0 and 1.2% at early blastocyst/blastocyst stages, 0, 6.8, 7.2, 15.3 and 2.0% at expanded blastocyst stage and 0, 1.6, 4.8, 19.3 and 38.5% hatching/hatched blastocyst stages, respectively. The peaks of early blastocyst/blastocyst, expanded blastocyst and hatching/hatched blastocyst stages were observed on Days 8, 9 and 10, respectively. The percentages of oocytes which initially became arrested and subsequently degenerated were 3.6, 4.8, 10.4, 14.5, 21.3 and 24.5% on Days 4, 6, 7, 8, 9 and 10 post-insemination, respectively.

• Journal of Plant Biotechnology
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• v.43 no.4
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• pp.492-499
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• 2016

This study assessed the whitening and anti-aging effects of the Cistanche deserticola extract, to develop a cosmetic substance. The cell viability of the Cistanche deserticola extract was evaluated in B16F10 melanoma cells by the MTT (3-(4,5-dimethylthaiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. The cell viability of the extract was determined to be 90% at 4mg/ml concentration. Furthermore, the tyrosinase, collagenase, and elastase mRNA expression level were measured by RT-PCR, using the Cistanche deserticola extract treated B16F10 melanoma cells. At 4 mg/ml concentration, mRNA expression level of tyrosinase, collagenase, and elastase was dramatically decreased to 80.9%, 37.6%, and 70.9%, respectively. The antioxidant activity of the Cistanche deserticola extract was determined by DPPH free radical scavenging. The DPPH free radical scavenging capacities ranged from 70.6% to 82.6%, when evaluated from 2 mg/ml to 10mg/ml concentrations. The effects of whitening and anti-aging of the Cistanche deserticola extracts were examined at 2, 4, 6, 8, and 10 mg/ml concentration. Tyrosinase activities were inhibited from 66.8% to 78.5%, elastase activities were inhibited from 67.6% to 79.3%, collagenase activities were inhibited from 72.3% to 83.6%, and hyaluronidase activities were inhibited from 65.8% to 69.2%, respectively. These data suggest that the Cistanche deserticola extract is effective in whitening and anti-aging; therefore, it is considered to be a functional cosmetic material in cosmetic products.

• The Journal of Industrial Distribution & Business
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• v.9 no.3
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• pp.31-43
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• 2018

Purpose - With the technology of the 4th industrial revolution, business models of agricultural sector are changing rapidly toward convergence and high value-added business models due to the 6th industrialization of agricultural. Our goals is to examine the 6th industrialization of agriculture, and then to apply the technology of the 4th industrial revolution to the 6th industrialization of agriculture, suggesting the possibility of future agriculture, and then linking the export competitiveness of agricultural products. Research design, data, and methodology - As the methodology, case studies and empirical analyzes were conducted as well as literature studies. The case analyses included tomatoes, pig breeding farms, and an empirical analysis was conducted using the AHP analysis by experts of the 6th industrialization. In addition, using 124 survey data, this study examined and analyzed the items of the 4th industrial revolution technology for the 6th industrialization of agriculture and the strengthening of export competitiveness. Results - Results showed that the technology of the 4th Industrial Revolution helped "6th industrialization of agriculture" and "the strengthening of export competitiveness" using two successful cases. The AHP analysis was also carried out, and it was found that the improvement of the technology in the 4th industrial revolution could contribute to the future industrialization as well as the 6th industrialization of agriculture. First of all, we looked many conditions were important and urgent. Among the technologies of the 4th industrial revolution, the mobile, big data were important. Moreover, it was recognized that linkage and convergence related efforts would greatly contribute to strengthening export competitiveness of agriculture such as price and quality competitiveness. Conclusions - The 4th industrial revolution such as hyper-connectivity, hyper-intelligence and hyper-predictability contribute greatly to the 6th industrialization of agriculture, and therefore it is essential to improve the competitiveness of the agricultural sector by using the technology of the 4th industrial revolution. In particular, based on analyses of the diamond model, the "demand conditions" was the most important factor for the activation of the 6th Industrialization, and then "related and supporting fields", "factor conditions" and "business context" were followed in order. The results of this study can be useful for policy, practical and academic sectors.