• Title/Summary/Keyword: 6-03

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Toxicity and Characteristics of Antifungal Substances Produced by Bacillus amyloliquefaciens IUB158-03 (Bacillus amyloliquefaciens IUB158-03이 생산하는 항진균물질의 생화학적 특성 및 독성)

  • Kim, Hye-Young;Lee, Tae-Soo
    • Journal of Life Science
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    • v.19 no.11
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    • pp.1672-1678
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    • 2009
  • The purified antifungal substances produced by Bacillus amyloliquefaciens IUB158-03 was positive to ninhydrin but negative to aniline, suggesting that the antifungal substance could be a peptide. FAB-MS, UV adsorption spectrum, and amino acid composition analysis revealed that the molecular weight of the antifungal substance was 1042 and that maximal adsorption was at 220 nm and 277 nm. The antifungal substance was composed of $Asn_3$, $Gln_2$, $Ser_1$, $Gly_1$, and $Tyr_1$. The composition and structural characteristics of antifungal substance were analysed by $^1H$-NMR spectrum, $^1H$-COSY, HMQC, which revealed that the compound belongs to the iturin A family. Temperature and pH had little effect on the stability of the antifungal substance in the ranges of $-70{\sim}121^{\circ}C$ and pH 6.0~10.0, respectively. It showed strong antibiotic activity against fungi. An in vitro cytotoxicity test using NIH3T3 cell showed that the antifungal substance does not have cytotoxicity. The number of circulating leukocytes and the hematobiological analysis of the mice administered with the antifungal substances was similar to those of the control group, indicating no cytotoxicity in vivo. Therefore, the antifungal substances extracted from culture broth of Bacillus amyloliquefaciens IUB158-03 have future potential as biocontrol agents against plant diseases caused by fungi.

Effects of doping on the electrical conductivity and particle size in olivine type $LiFePO_4$ powders (올리빈형 $LiFePO_4$ 분말의 전기전도도와 입도 크기에 미치는 도핑의 영향)

  • Bai, Jin-Tao;Ha, Jung-Soo;Kim, Chang-Sam
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.18 no.6
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    • pp.248-252
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    • 2008
  • To get a fine $LiFePO_4$ powder with high electrical conductivity, the influences of doping of aliovalent elements(Cr+B and Cr+Al) on electrical conductivity and of heat treatment conditions on particle size of the doped powders were studied. Two kinds of the doped powders $LiFe_{0.965}Cr_{0.03}B_{0.005}PO_4$ and $LiFe_{0.065}Cr_{0.03}Al_{0.005}PO_4$ were synthesized using mechanochemical milling and subsequent heat treatment at $675{\sim}750^{\circ}C$ for $5{\sim}10\;h$. The doping enhanced grain growth and electrical conductivity. The electrical conductivity at $30^{\circ}C$ was $1{\times}10^{-8}S/cm$ in the doped with Cr and Al, and $5{\times}10^{-10}S/cm$ in the undoped one.

A Study on the strength of mechanically fastened composite joint (기계적으로 체결된 복합재료 조인트의 강도에 관한 연구)

  • 최진호;전영준;권진희
    • Composites Research
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    • v.15 no.4
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    • pp.9-16
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    • 2002
  • With the wide application of fiber-reinforced composite material in aero-structures and mechanical parts, the design of composite joint have become a very important research area because they are often the weakest areas in composite structures. In this paper, the failure area index method to predict the strength of the mechanically fastened composite joint which has the same stacking sequence was suggested and evaluated. By the suggested failure area index method, the strength of the mechanically fastened composite joint could be predicted within 6.03%.

Corrosion and Repassivation Behavior of Stainless steels in Chloride and Thiosulfate Containing Environments

  • Wang, Y.S.;Singh, P.M.
    • Corrosion Science and Technology
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    • v.14 no.4
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    • pp.184-189
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    • 2015
  • In this study, the combined effect of chloride and thiosulfate ions and the effect of the ratio of the two ions on passivation in 304L, 316L, and the duplex stainless steels 2101 and 2205 are investigated using potentiostatic scratch tests. Cyclic polarization and the scratch tests were used to understand the role of anions on localized corrosion in these systems. It was found that the thiosulfate pitting began at a lower potential for 2101 than 304L in 0.6 M NaCl + 0.03 M $Na_2S_2O_3$ solution. The pit morphologies for 304L, 316L, and 2101 in an 0.6 M NaCl + 0.03 M $Na_2S_2O_3$ solution were very different from each other. The results indicate that the pitting switches from predominately thiosulfate pitting to chloride pitting at approximately 0.1 V.

Study on the Age Determination in Korean Adult Teeth by Gustafson에s Method (Gustafson 방법에 의한 한국인 영구치에서의 연령감정에 관한 연구)

  • 김동원;김종열
    • Journal of Oral Medicine and Pain
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    • v.9 no.1
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    • pp.103-116
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    • 1984
  • Randumly sampled out 157 extracted Korean adult teeth, aged 12 to 79 years, was applicated to estimat the age by Gustafson's method, were evaluated and analyzed in terms of phyological aging change. The results are as follows : 1. It was reconfirmed that there existed comparatively close correlationship between age and score, especially in old age. 2. Korean score had comparatively high level than those of European. 3. In the probability of age edetermination, the estimation error was the most high level in the age of 70~ with ±4.47, and next order was in the age of 10-19 with ±5.20,60-69 with ±5.8,30-39 with ±5.98,50-59 ±6.20,40-49 with ±6.72,20-29 with ±9.28.Mean value was ±8.03. 4. The regression equation is as follows. y=3.55x+8.52(r=8.75) standard deviation σ=±8.03 (y= estimated age x=score) 5. In age determination with those data, needs to estimate slightly lower than caculated age in range of 10-49, and higher in 50-70 for the real age determination.

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Comparison of the Sulfur Dioxide Primary Standard Gases of NPL and KRISS

  • O, Sang Hyeop;Kim, Byeong Mun;Mun, Dong Min;Kim, Jin Seok
    • Bulletin of the Korean Chemical Society
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    • v.22 no.12
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    • pp.1341-1344
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    • 2001
  • Comparison of sulfur dioxide primary standard gases of the Korea Research Institute of Standards and Science (KRISS, Korea) and the National Physical Laboratory (NPL, UK) was performed. 100 ${\times}$10-6 mol/mol and 1,000 ${\times}$10-6 mol /mol primary standard gases (designated NPL S115 and S114, respectively) prepared gravimetrically and validated in NPL were used as transfer standards. Transfer standards were analyzed by NDIR sulfur dioxide analyzer and compared with KRISS PSM 112-03-624 and PSM 112-03-625 prepared gravimetrically. Adsorption corrected relative deviations of the primary standard gases were agreed to within 0.1%, and this agreement is within the expanded uncertainties (k = 2) of the primary standards at the two laboratories.

Studies on Wax Gourd Wine (다이어트와인 동아주 제조에 관한 연구)

  • 안용근;신상철;김승겸;신철승
    • The Korean Journal of Food And Nutrition
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    • v.13 no.6
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    • pp.578-584
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    • 2000
  • Three kinds of the mashes of 15%, 20% and 25% of boiled wax gourd containing 20% of sugar were fermented by yeast at 5~1$0^{\circ}C$ for 100 days. After fermentation, total sugar content of the 15% mash was 8.0%, 20% mash 8.6%, and 25% mash 8.3%, respectively. Also, it revealed that in reducing sugar content, 15% mash was 7.4%, 20% mash 7.6%, and 25% mash 7.4%, respectively, In protein content, 15% mash was 10.3mg/ml, 20% mash 9.8mg/ml, and 25% mash 11.3mg/ml, and in amino acid content, 15% mash was 0.13 $\mu$mo1/ml, 20% mash 0.03 $\mu$mo1/ml, and 25% mash 0.03 $\mu$mo1/ml, each. In case of pH, 15% mash was 3.88, 20% mash 3.99, and 25% mash 3.97, respectively. In acidity, 15% mash was 0.37, 20% mash 0.44. and 25% mash 0.43, respectively. In the number of yeast cell, 15% mash was 8.33 logCFU/ml, 20% mash 8.56 logCFU/ml, and 25% mash 8.57 logCFU/ml. respectively. In ethanol content, 15% mash was 13.4%, 20% mash 14.9%, and 25% mash 15.5%, respectively. In organic acid content, acetic acid, pyruvic acid, citric acid, and lactic acid were produced, and succinic acid content ranges from 1,407~1,800 mg/l. Five-grade scoring test of sensory evaluation showed that in its taste degree, 15% mash was 3.93, 20% mash 3.66 and 25% mash 3.40, respectively.

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전기안전사고사례 - 발전기 교체 작업중 메탄가스 폭발로 1명 사망, 6명 부상한 사고 발생

  • Ryu, Bo-Hyeok
    • Electric Engineers Magazine
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    • s.358
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    • pp.44-45
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    • 2012
  • 2012년 03월 16일(금) 11:05경, 경기도 고양시 소재 ${\bigcirc}{\bigcirc}$물재생센터의 발전기(3기)를 교체하기 위하여 발전기 및 관련 설비를 해체하는 과정에서 가스 압축기실 내에 있던 누출된 메탄가스가 배관 등의 용단작업 중에 발생된 불꽃에 의해 점화 폭발로 1명 사망, 6명이 부상당한 재해임.

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Water Quality of Some Spring Waters in Pusan Area (부산시내에 산재하는 몇몇 약수터 약수의 수질)

  • KIM Yong-Gwan;CHO Hyeon-Seo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.18 no.6
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    • pp.538-544
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    • 1985
  • This study was carried cut to evaluate the water quality of spring waters in Pusan area(see Fig. 1). In this experiment, twenty-five water samples were collected from 5 stations from December 1983 to August 1984. Range and mean values of constituents of the samples are as follows: pH $5.80{\sim}7.25$, 6.60; water temperature $6.0{\sim}23.0^{\circ}C,\;12.9^{\circ}C$; total residue $33.0{\sim}325mg/l$, 121.2mg/l; alkalinity $4.75{\sim}51.6mg/l$, 24.1mg/l; hardness $9.47{\sim}85.0mg/l$, 30.3mg/l; electrical conductivity $0.495{\sim}2.750{\times}^2{mu}{\mho}/cm,\;1.239{\times}10^2{\mu}{\mho}/cm$;turbidity $0.54{\sim}7.80$NTU, 2.04NTU; $KMnO_4$ consumed $0.51{\sim}8.47mg/l$, 1.96mg/l; chloride ion $4.91{\sim}36.0mg/l$, 12.55mg/l; fluoride ion ND-0.30ppm, 0.08ppm; nitrate-nitrogen ND-8.94mg/l, 1.94m:g/l; nitrite-nirogen ND-0.10mg/l, 0.03mg/l; ammonia-nitrogen ND-0.16mg/l, 0.03mg/l: phosphate-phosphorus ND-0.09mg/l, 0.03mg/l; silicate-silicious $0.42{\sim}22.7ng/l$, 7.96mg/l; copper ND-10.5ppb, 2.46ppb; lead ND-22.7ppb, 3.54ppb; zinc ND-103ppb, 21.33ppb; iron $20.3{\sim}2,800ppb$, 801.72ppb, respectively. Arsenic, cyan, cadmium, manganese, mercury, chrome and phenol were not detected. Total residue, electrical conductivity, turbidity and chloride ion of station 1 (Milrakdong) were higher than others as 178.1mg/l, $2.127{\times}10^2{\mu}{\mho}/cm$, 3.16NTU and 16.32mg/l. The concentration of silicious had a great influence on precipitation. The concentration of fluoride ion of spring waters was lower as 0.08ppm than the criterion for drinking water as 1ppm, while iron was exceed 2.7 times as 801.72ppb.

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초점/경유승용차 허용 논란 - 경유차 대기오염 저감대책

  • Korea LPGas Industry Association
    • LP가스
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    • s.83
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    • pp.40-42
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    • 2003
  • 민관 합동으로 구성된 경유차 환경위원회는 '03.2.6 환경정책평가연구원에서 "경유차 대기오염 저감대책 공개토론회"를 개최하고 에너지가격체계 조정 등 경유차 대기오염 저감대책을 발표했다.

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