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Migration Mechanism of Hazard Elements from Brass Kitchenwares (황동제 주방기구에서 유해중금속의 용출기작에 관한연구)

  • Lee, Kwang-Ho;Kwon, Ki-Sung;Jeon, Dae-Hoon;Choi, Byung-Hee;Kim, Sung-Wook;Lee, Sun-Hee;Lee, Chul-Won
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.5 no.2
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    • pp.24-30
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    • 1999
  • The migration mechanism of trace elements, Pb, Cd, Cu, Zn and Sn from brass food-contact utensiles was investigated. The migration of metals from brass was affected by the migration temperature (30, 60, 80 and $95^{\circ}C$), the simulant pH (2.5, 4.3, 6.0 and 7.0) and the migration time (30, 90, 180 and 360 min.). The amount of Pb migration was maximum at $95^{\circ}C$ with pH 2.5. This study indicated that the equilibrium of Pb migration was reached at 180 minutes. The mechanism of Pb migration was investigated with Scanning Electron Microscope (SEM) and Electron Probe Micro Analyzer (EPMA). To correlate the relations between the migration and the content of Pb in brass samples, ten brass samples having known content of Pb (the quantities of Pb in brass ; 0, 0.02, 0.09, 0.1, 0.2, 0.5, 0.9, 1.4, 5.4 and 9.2% (w/w)) were tested in the following conditions, 4% acetic acid, $95^{\circ}C$ and 30 minutes. The result represented that content of Pb in brass samples must be below 0.2% (w/w) to satisfy the 1.0 ppm (as Pb) of Korea Food Code.

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Effect of Nonionic Surfactant Solutions on Wetting and Absorbency of Polyethylene Terephthalate(PET) Fabrics (Part II) -Surfactants Characteristics and Fabric Properties- (비이온계 계면활성제 수용액이 PET직물의 습윤특성에 미치는 영향 (제2보) -계면활성제와 직물의 특성-)

  • Kim, Chun-Hee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.29 no.12 s.148
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    • pp.1546-1553
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    • 2005
  • The wetting behavior and liquid transport of nonionic surfactant solutions; Span 20 and Tween 20, 40, 60, 80, 21, 61, 81, 65 & 85: in polyethylene terephthalate(PET) fabrics are reported. Five different PET fabrics are used in this study. PET 1, 2 & 3 have different compactness in structure. PET 4 & 5 have similar physical properties to PET 2, however, PET 4 has heat set finish and PET 5 with rewetting agent. The wetting and water retention properties of PET fabrics are greatly improved by addition of nonionic surfactants. The aqueous liquid retention(W) vs. cosq and W vs. adhesion tension has positive linear relationship. Hydrophilic surfactants which have short hydrophobes and surfactants with unsaturated hydrophobe structures are more efffctive in improving the wetting properties of PET fabrics. PET fabric which has larger thread spacing shows greater value of water retention ratio(W/H) than PET fabric with smaller thread spacing if there are no surfactants present in the system, however, W/H values become very similar among these PET fabrics when the surfactants are added. If there are no surfactants present in the system, PET with heat set finish has smaller value and PET with rewetting agent has greater value of W/H than PET without finish even though the fabrics have the similar physical properties.

Properties of Bac W42, a Bacteriocin Produced by Bacillus subtilis W42 Isolated from Cheonggukjang

  • Kindoli, Salum;Lee, Hwang A;Kim, Jeong Hwan
    • Journal of Microbiology and Biotechnology
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    • v.22 no.8
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    • pp.1092-1100
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    • 2012
  • Ten Bacillus strains with antimicrobial activities were isolated from Cheonggukjang produced at different parts in Korea. They all inhibited Listeria monocytogenes ATCC 19111 and nine inhibited Bacillus cereus ATCC 14579. Four isolates (W42, H27, SKE 12, and K21) showing strong inhibiting activities were identified as B. subtilis. B. subtilis W42 was the most inhibiting strain. The antimicrobial activity of culture supernatant from B. subtilis W42 was destroyed completely by proteinase K treatment, indicating that a bacteriocin was the responsible agent. The bacteriocin, Bac W42, was most stable at pH 7 and stable between pH 3-6 and 8-9. Bac W42 was stable up to $80^{\circ}C$. BHI (brain heart infusion) and TSB (tryptic soy broth) were the best media for the activity (320 AU/ml) followed by LB (160 AU/ml). Bac W42 was partially purified by column chromatographies. The specific activity was increased from 1,151.2 AU/ml to 9,043.5 AU/ml and the final yield was 26.3%. Bac W42 was 5.4 kDa in size as determined by SDS-PAGE. Bac W42 showed bactericidal activity against L. monocytogenes ATCC 19111.

A Study on the Cutting Optimal Power Requirements of Fast Growing Trees by Circular Saw (원형톱에 의한 속성수 절단 적정 소요동력 산정에 관한 연구)

  • Choi, Yun Sung;Kim, Dae Hyun;Oh, Jae Heun
    • Journal of Korean Society of Forest Science
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    • v.103 no.3
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    • pp.402-407
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    • 2014
  • In this study, Italy poplar(Populus euramericana) was selected for test specimen to measure cutting power when it harvested. The experiment has been controlled as three levels of feed rate (0.41, 1.25 and 2.5 m/s), sawing speed (800, 1,000 and 1,200 rpm), and the five levels of root collar diameter (50, 70, 90 and 110, 130 mm). The harvested volume after 3 years (root collar diameter 50 mm) was 10.5 tons, which falls short of the target amount of biomass is 20~30 ton/ha. In addition, the biomass amount of diameter 90 and 110 mm which reached the target amount were estimated to be 23.5 and 32.5 ton/ha respectively. As a result of experiment, it was found out that power of 128.2 and 175.8 W are consumed in case of cutting with the feed rate of 0.41m/s and minimum sawing speed (800 rpm) respectively. With the working area of 0.3 ha/h, it is considered to present working capacities of 16.5 and 22.8 ton/h respectively. The power consumed at the feed rate of 1.25 m/s is estimated to be 113.8 and 153.7W respectively and working capacity in a working area of 1 ha/h is estimated to be 23.5 and 32.5 ton/h. The power consumed at the feed rate of 2.5 m/s is estimated to be 119.8 and 166.9 W respectively and working capacity in a working area of 2 ha/h is estimated to be 47.0 and 65.5 ton/ha respectively. Therefore, the power source of harvest machine at the feed rate of 1.25, 2.50 m/s and sawing speed of 800 rpm shall be selected as it can process the target amount of estimated biomass.

Anti-Obesity Effect of Fermented Pollack Skin and Wolfiporia extensa Ginns in High Fat-induced Obesity Animal Model (복령과 황태껍질 발효물 혼합물의 고지방식이 유도 비만 동물모델에서의 항비만효과)

  • Mi Ja Kim;Soon Ah Kang
    • The Korean Journal of Food And Nutrition
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    • v.36 no.5
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    • pp.403-414
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    • 2023
  • This study aimed to determine the anti-obesity effect of adding Wolfiporia extensa Ginns (W) to fermented pollack skin products in an obesity-induced animal model. The experimental groups were the normal diet group (C), high-fat diet group (HF), dried pollack skin (H1), fermented pollack skin (H2), and W of 0.1 (F2-WL), 0.3 (F2-WM), and 0.5 (F2), respectively. It was confirmed that adding W to fermented pollack skin reduced blood triglycerides, total cholesterol, and LDL levels, while increasing HDL levels. Wolfiporia extensa Ginns was effective in controlling weight and improving blood lipids in a dose-dependent manner. In histological analysis, findings of fatty liver induced by a high-fat diet were improved by the addition of H2 and W. Size and density of fat globules in the epididymis were decreased. In addition, the concentration of TNF-α was increased in the high-fat diet group, but decreased by the addition of fermented pollack skin and W. In conclusion, adding fermented dried pollack skin and Wolfiporia extensa Ginns was effective for weight control and blood lipid improvement. Thus, the use of by-products in functional foods is expected to have a high value in the future.

Evaluation of in-vitro Antithrombosis Activity of Lees of Korean Traditional Wine (전통주 주박의 항혈전 활성 평가)

  • Kim, Mi-Sun;Lee, Ye-Seul;Kim, Jong Sik;Shin, Woo-Chang;Sohn, Ho-Yong
    • Journal of Life Science
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    • v.24 no.8
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    • pp.865-872
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    • 2014
  • In this study, ethanol and hot water extracts of lees from Korean traditional wine (J-B, J-S, J-Y, J-H, and J-W) were prepared, and their effects on blood coagulation, platelet aggregation, and hemolysis of human red blood cells (hRBCs) were investigated to develop functional food ingredients from lees. The pH and brix of the lees ranged from 3.90 to 4.29 and 5.0 to $27.0^{\circ}$, respectively, and there was a huge difference in the water and ethanol content among the lees. The nuruk and additives used affected the color and physicochemical properties of lees. The J-W takju made from only rice and traditional nuruk, which has $13^{\circ}$ brix and 1.8% of alcohol, has potential as functional food ingredient. With regard to the extraction yields of lees, higher yields were obtained from J-H, which contains different medicinal plants, in ethanol, followed by J-W, J-B, J-S, and J-Y. Higher extraction yields of lees were obtained from J-S in hot water, followed by J-B, J-W, J-H, and J-Y, respectively. The ethanol extract of J-H and the hot water extract of J-Y had the highest contents of total polyphenol and total flavonoids among the lees extracts. The 10 lees extracts did not show hemolysis activity against hRBCs up to 5 mg/ml. In an anticoagulation activity assay, the ethanol extracts of three yakju lees (J-B, J-S, and J-Y) and the hot water extract of J-W inhibited thrombin activity, whereas the hot water extract of J-B, J-S, and J-H inhibited blood coagulation factors. In an antiplatelet aggregation activity assay, only the J-W takju lees showed significant inhibition activity. Our results suggest that lees from traditional wine had high potential as a novel antithrombosis agent.

Development of Optimum Process for Continuous Hydrolysis of Fish Skin Gelatin Using a Three-Step Recycle Membrane Reactor (재순환 3단계 막반응기를 이용한 어피젤라틴의 연속적 가수분해 최적화 공정 개발)

  • Kim, Se-Kwon;Byun, Hee-Guk
    • Applied Chemistry for Engineering
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    • v.5 no.4
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    • pp.681-697
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    • 1994
  • The enzymatic hydrolysate of gelatin extracted from fish skin was fractionated and recycled through the membrane reactor according to the molecular weight for the purpose of using as functional material. In addition, the enzymatic hydrolysis conditions of gelatin, enzyme stability by membrane and mechanical shear, and effect on the long-term operational stability of the recycle membrane reactor were investigated. Using the pH-drop technique, Alcalase, pronase E and collagenase were identified as the most suitable enzymes for the hydrolysis of fish skin gelatin. The optimum hydrolysis conditions in the 1st-step membrane reactor(1st-SMR) by Alcalase were enzyme concentration 0.2mg/ml, substrate-to-enzyme ratio(S/E) 50(w/w), $50^{\circ}C$, pH 8.0, reaction volume 600ml and flow rate 6.14ml/min. In the 2nd-SMR by pronase E were enzyme concentration 0.3mg/ml, S/E 33(w/w), $50^{\circ}C$, pH 8.0, reaction volume 600ml and flow rate 6.14ml/min. In the case of 3rd-SMR, enzyme concentration 0.1mg/ml, S/E 100(w/w), $37^{\circ}C$, pH 7.5, reaction volume 600ml and flow rate 10ml/min. Decreased enzyme activities by mechanical shear and membrane were 30% and 15% in the 1st-SMR, were 14% and 5% in the 2nd-SMR, and 18% and 8% in the 3rd-SMR, respectively. Under the optimum conditions, the degree of hydrolysis in the 1st, 2nd and 3rd-SMR were 3.5%(Kjeldahl method, 87%), 3.1%(77%) and 2.7%(70%), respectively. The productivity of hydrolysate in the continuous three-step membrane reactor was 430mg per enzyme(mg) for 10 times of volume replacements.

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Recovery of IGF-I Using Liquid Emulsion Membranes (액막법을 이용한 IGF-I 회수)

  • 최광수;문용일
    • Korean Journal of Animal Reproduction
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    • v.22 no.1
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    • pp.89-94
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    • 1998
  • A study was made to investigate the effects of concerning factors with IGF-I recovery on the final IGF-I concentration in the effluent and to establish recovery conditions of IGF-I using liquid emulsion membranes(LEM). D2EHPA was best carrier among Amberlite LA2, Aliquit 336 and D2EHPA for recovery rate of IGF-I. Recovery rate of IGF-I by D2EHPA volume in the oil phase was increased as increasing D2EHPA volume, and optimal volume of D2EHPA was 5% in this experiment. The recovery rate of IGF-I by D2EHPA was increased by the decreasing from pH 7 to pH 4 of external phase. Therefore, optimal pH value was 4.0. Optimal concentrations of sulfuric acid in internal phase, paraffin oil in oil phase and Span 80 for recovery rate of IGF-I were 0.1M, 2.0% and 5%, respectively, and optimal W/O rate was 2. These results suggested that optimal conditions for recovery of IGF-I were D2EHPA(5%) as carrier, pH 4.0, 0.1M sulfuric acid, 2% paraffin oil, 2.0 W/O rate and 5.0% Span 80.

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Purification and Characterization of Collagenase Produced by Staphylococcus aureus JJ-11 Isolated from the Human Skin (피부에서 분리한 Staphylococcus aureus JJ-11이 생산하는 collagenase의 정제 및 특성)

  • Lee Jin-Kyoung;Kim Hae-Nam;Kang Ho-Young;Jun Hong-Ki
    • Journal of Life Science
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    • v.16 no.2 s.75
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    • pp.245-252
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    • 2006
  • A bacterial strain, identified as Staphylococcus aureus JJ-11, producing collagenase was isolated out of 40 persons having skin troubles. S. aureus JJ-11 produced collagenase optimally in the media containing 1.5%(w/v) gelatin, 1%(w/v) yeast extract, 0.4%(w/v) $K_2HPO_4$, 0.005%(w/v) $NiSO_4{\cdot}6H_2O$ at $37^{\circ}C$ for 18 hrs. The collagenase produced by Staphylococcus aureus JJ-11 was purified at 6.66-folds purity through application of chromatography with Amberlite IRA-900 and Sephacryl S-300 HR columns. The molecular weight of the partially purified enzyme was estimated to be 62 kDa by SDS-PAGE. The protein exhibited optimum enzymatic activity at pH 7.0, and showed a stable activity at pH 4-8. The optimum temperature for collagenase was at $37^{\circ}C$, and activity was maintained upto $40^{\circ}C$. The enzyme activity was slightly elevated in the presence of divalents such as, $Fe^{2+},\;Co^{2+}\;and\;Ba^{2+}$ However, the activity was inhibited in the presence of $Sr^{2+}\;or\;Hg^{2+}$. The inhibition of activity by O-phenanthroline and EDTA suggested that the enzyme may contain metal which is required for activity. The enzyme showed the highest activity when insoluble collagen (type I) was, used as a substrate.

Development of a Rice Circulating Concurrent-flow Dryer (I) - Performance Test of Pilot Scale Dryer - (순환식 병류형 곡물건조기 개발 (I) -시작기의 성능시험 -)

  • Han J.W.;Keum D.H.;Han J.G.;Kim H.;Hong S.J.
    • Journal of Biosystems Engineering
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    • v.31 no.4 s.117
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    • pp.349-354
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    • 2006
  • This study was performed to evaluate the performances for a concurrent flow rice dryer of pilot scale with devices for circulating rice. The pilot scale dryer with the capacity of 700 kg was developed to obtain design informations for the development of actual scale dryer of holding capacity of 10 tons. Three drying tests were conducted at two temperature levels of $100^{\circ}C\;and\;120^{\circ}C$, and two air flow rates levels of $28.5cmm/m^2\;and\;57.1cmm/m^2$. Drying conditions for Test-1, Test-2 and Test-3 were $100^{\circ}C\;-28.5cmm/m^2,\;120^{\circ}C-28.5cmm/m^2\;and\;120^{\circ}-57.1cmm/m^2}$ respectively. Drying rates were 0.73%(w.b./h) for Test-1, 0.90%(w.b./h) for Test-2 and 1.46%(w.b./h) for Test-3. The crack ratios of brown rice after drying ranged from 2.4% to 8.4%, and increased with the increase of drying rate and airflow rate. The energy consumptions were from 6,225 kJ/kg to 6,993 kJ/kg which was higher than that of conventional cross-flow rice circulating type dryer used in Korea. This results were due to the lower ambient air temperatures of $4.5^{\circ}C\;to\;13.4^{\circ}C$ during drying tests.