• The Korean Journal of Pain
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• v.35 no.4
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• pp.423-432
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• 2022

Background: The decreased expression of mu-opioid receptors (MOR) in the amygdala may be a key molecular in chronic post-surgical pain (CPSP). It is known that miR-339-5p expression in the amygdala of a stressed rat model was increased. Analyzed by RNAhybrid, miR-339-5p could target opioid receptor mu 1 (oprm1) which codes MOR directly. So, the authors hypothesized that miR-339-5p could regulate the expression of MOR via targeting oprm1 and cause the effects to CPSP. Methods: To simulate perioperative short-term stress, a perioperative stress prolongs incision-induced pain hypersensitivity without changing basal pain perception rat model was built. A pmiR-RB-REPORT^TM dual luciferase assay was taken to verify whether miR-339-5p could act on oprm1 as a target. The serum glucocorticoid level of rats was test. Differential expressions of MOR, GFAP, and pERK1/2 in each group of the rats' amygdala were tested, and the expressions of miR-339-5p in each group of rats' amygdalas were also measured. Results: Perioperative stress prolonged the recovery time of incision pain. The expression of MOR was down-regulated in the amygdala of rats in stress + incision (S + IN) group significantly compared with other groups (P < 0.050). miR-339-5p was up-regulated in the amygdala of rats in group S + IN significantly compared with other groups (P < 0.050). miR-339-5p acts on oprm1 3'UTR and take MOR mRNA as a target. Conclusions: Perioperative stress could increase the expression of miR-339-5p, and miR-339-5p could cause the expression of MOR to decrease via targeting oprm1. This regulatory pathway maybe an important molecular mechanism of CPSP.

• Bulletin of the Korean Chemical Society
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• v.20 no.5
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• pp.619-620
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• 1999

• Proceedings of the KIPE Conference
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• 2003.07b
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• pp.977-980
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• 2003

오늘날 청정에너지 중에서 많은 주목받고 있는 것으로 연료전지를 들 수 있다. 그러나 연료전지의 경우 저전압 대전류의 특성으로 인하여 상용전원에 전원을 공급하기 위해서는 승압 및 상용주파수로 변환해야하는 중간 과정을 거쳐야 한다. 이러한 전력변환장치로 1.5kW를 제작하고 실험으로서 전력 변환장치의 타당성을 검증 하고자 한다.

• International Journal of Air-Conditioning and Refrigeration
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• v.16 no.2
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• pp.44-50
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• 2008

This study presents test results of a mobile air-conditioning system using a potential alternative refrigerant, R152a. A series of performance tests have been carried out and cycle characteristics such as cooling capacity, energy efficiency ratio, suction and discharge pressures, and temperatures are presented, compared to those for the baseline R134a system. Tests were conducted with evaporation temperature of $5^{\circ}C$, condensation temperature of $45^{\circ}C$, subcooling temperature of $5^{\circ}C$, superheating temperature of $5^{\circ}C$, and compressor speed of 500-1500 rpm. The performance of R152a system with readjustment of an expansion valve showed better than those of R134a. The effect of oil on the pressure drop in the evaporator was also addressed.

• Information and Communications Magazine
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• v.31 no.1
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• pp.23-28
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• 2013

2020년 5세대 이동통신 상용화를 목표로 중국, 일본, 유럽 등 주요국은 기술, 표준화, 시장 주도를 위해 경쟁적으로 연구 개발 및 투자를 본격화하고 있다. 우리나라도 국가 성장동력을 창출해온 이동통신분야에서 새로운 기술 진화 및 혁신으로 선제적으로 대응하기 위해 5G를 10대 ICT기술로 선정하고, 도전적 R&D 추진 계획을 수립하였다. 이에 본고에서는 이동통신 시장 및 표준화 현황을 살펴보고, 미래부의 'ICT R&D 중장기 전략' 및 '5G 이동통신 추진계획'에 따른 5G 중장기 목표 및 '14년도 신규과제 기획방향에 대해 알아본다.

• Natural Product Sciences
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• v.29 no.1
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• pp.10-16
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• 2023

We previously reported that dried Lysimachia christinae whole plant extract exerted significant neuroprotective activity. In this study, we tried to isolate neuroprotective compounds of L. christinae. We evaluated the neuroprotective activity of the four fractions (hexane, chloroform, ethyl acetate, and n-butanol fractions) of methanol extract. Among them, ethyl acetate and n-butanol fractions showed most potent neuroprotective activity against glutamate excitotoxicity. Nine compounds were isolated from ethyl acetate and n-butanol fractions of L. christinae extract and identified as cynaroside (1), (3,4,5,6-tetrahydroxytetrahydro-2H-pyran-2-yl)methyl-3-hydroxy-2-octyldopentaconta-23,33-dienoate (2), androst-16-ene-3,6-diol (3), 2-hydroxy-24-propoxytetracos-4-enoic acid (4), 2-hydroxy-24-methoxytetracos-4-enoic acid (5), 12-(stearoyloxy)octadec-9-enoic acid (6), β-sitosterol (7), (E)-4-(3,4-dimethoxyphenyl)but-3-en-1-yl palmitate (8) and (1S,2S,3R,4R)-4-(((2S,3R,4R,5R,6S)-2-(((2R,3R,4S,5R,6R)-2-(3,4-dimethoxyphenethoxy)-3,5-dihydroxy-6-(hydroxymethyl) tetrahydro-2H-pyran-4-yl)oxy)-4,5-dihydroxy-6-methyltetrahydro-2H-pyran-3-yl)oxy)cyclohexane-1,2,3-triol (9) by spectroscopic data such as UV, IR, NMR, Mass spectroscopy. Their neuroprotective activity was evaluated by MTT assay. Cynaroside (1) and androst-16-ene-3,6-diol (3) had significant neuroprotective activity against glutamate-injured HT22 cells. The neuroprotective efficacy of cynaroside (1) and androst-16-ene-3,6-diol (3) was related to their anti-oxidative activity.

• Journal of the Korean Ceramic Society
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• v.45 no.5
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• pp.290-296
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• 2008

$AB'_{0.5}B"_{0.5}O_3$ type complex perovskite structures which have been reported as proton conductors over $600^{\circ}C$ were studied. The $AB'_{0.5}B"_{0.5}O_3$ type complex perovskite structure is known to be more easily synthesized and has better stability than normal $ABO_3$ perovskite structure. And it is stable at about $800^{\circ}C$ in the $CO_2$ atmosphere, whereas the $BaCeO_3$ perovskite is easily decomposed into carbonate. In addition, this $AB'_{0.5}B"_{0.5}O_3$ type complex perovskite structure could simply produce oxygen vacancies within their structure not by introducing additional doping oxides but by just controling the molar ratio of $B'^{+3}$ and $B"^{+5}$ metal ions in the B site. Hence it is easy to design the structure which shows highly sensitive electrical conductivity to humidity. In this study, the single phase boundary of $BaR_{0.5+x}Ta_{0.5-x}O_{3-{\delta}}$(R = rare earth) complex perovskite structures and it's phase stability were investigated with changes in composition, x. And the humidity dependance of electrical conductivity at different $P_{H2O}$ conditions was investigated.

• Journal of Microbiology and Biotechnology
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• v.29 no.5
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• pp.785-793
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• 2019

Black rot caused by Xanthomonas campestris pv. campestris (Xcc) is the most damaging disease in Brassica crops around the world. In this study, we developed a molecular marker specific to Xcc race 5. To do this, the available whole genome sequences of Xcc races/strains and Xc subspecies were aligned and identified a highly variable genomic region (XccR5-89.2). Subsequently, a primer set covering the 'XccR5-89.2' region was designed and tested against the genomic DNA of Xcc races/strains, Xc subspecies and other plant-infecting bacterial strains (Pseudomonas syringae pv. maculicola and Erwinia carotovora subsp. carotovora). The results showed that the 'XccR5-89.2' primer pair amplified a 2,172-bp fragment specific to Xcc race 5. Moreover, they also amplified a 1,515-bp fragment for Xcc race 1 and an over 3,000-bp fragment for Xcc race 3. However, they did not amplify any fragments from the remaining Xcc races/strains, subspecies or other bacterial strains. The 'XccR5-89.2' primer pair was further PCR amplified from race-unknown Xcc strains and ICMP8 was identified as race 5 among nine race-unknown Xcc strains. Further cloning and sequencing of the bands amplified from race 5 and ICMP8 with 'XccR5-89.2' primers revealed both carrying identical sequences. The results showed that the 'XccR5-89.2' marker can effectively and proficiently detect, and identify Xcc race 5 from Xcc races/strains, subspecies and other plant-infecting bacteria. To our knowledge, this is the first report for an Xcc race 5-specific molecular marker.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.13 no.4
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• pp.7-13
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• 1999

The purpose of this study was to suggest the c1ata which were useful to apply the color for clothes resign 29 female university students were se10cted in this experirrental study for the perception scope of primroy color from July to Dec. in 1998. The results of this study are as the following : 1) The perception scope of red perceived by more than 30% of the subjects was wider in Chroma-axis than in Value-axis, including 5R 4/12, 5R 4/10 and 5R 5/14 in standard color 5R standard color 5R 4/14. The perception scope of yellow was wider in Chroma-axis in standard color 5Y 8/14. And the perception scope of blue was wider in Value-axis in standard color 5B 4/10. 2) In chromaticity diagram, the perception scope of red perceived by one subjoct and over was large, covering three-quarters and being extended in X -axis. The perception scope of yellow was awuximately a half, without being linked together. A few of it were separated. And the perception scope of blue was about two-thirds, including all color chips in the line. In conclusion, the perception scope of red was comprehensively broad and there aweared relatively strong corrnron feature between the subjects. The perception scq;e of yellow was awuximate1y a half and there was little cormnon feature between the subjects. And the perception sccpe of blue was sorrewhat wide and the sUbiects expressed strong cornrmn feature. feature.

• Journal of the Korean Chemical Society
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• v.37 no.2
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• pp.237-243
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• 1993

The primary and secondary structure of the 5S rRNA isolated from Xanthomonas celebensis were determined by enzymatic and chemical degradation methods. It consists of 119 nucleotides and contains no modified nucleosides. As with the 5S rRNAs of X. maltophilia and X. citri, it contains an additional uridine residue on the 5'-terminus. Its secondary structure was almost identical to the models previously proposed by us for the 5S rRNA of two Xanthomonas species. Its secondary structure consists of five helices, five loops and two bulges. The tertiary interactions in the 5S rRNA molecule were analyzed by Fe(II)-EDTA treatment and hybridization method using deoxyhexamer. From the fact that some adenine residues in loop M, region $I_1-C$, loop $H_1$, and loop $H_2$ become susceptible to diethylpyrocarbonate when the 5S rRNA was hybridized with deoxyhexamer complementary to the sequence $U_{35}CCCAU_{40}$ and that some nucleotide residues in loop M, loop $H_1$ and region $D-I_2$ become resistant Fe(II)-EDTA cleavage in the presence of $Mg^{2+}$, it is presumed that loops $H_1$ and $H_2$ interact with loop M in some way. In the tertiary interaction, the regions $I_1-C$ and $D-I_2$ seem to act as hinges in folding the stems $B-I_1-C$ and $D-I_2-E.$ It was found that loop $H_1$ changes into a smaller loop of three bases by forming noncanonical A : C base-pairs ih acidic environment.