• Journal of the Korean Chemical Society
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• v.19 no.2
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• pp.98-103
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• 1975

The synthesis of two new ligands, 1,10-dibenzyltriethylenetetraamine (1,10-$Be_2trien$) and 1,10-dibenzyl-5R-methyltriethylenetetraamine (1,10-$Be_2$-5R-Metrien), and the preparation of the dichloro cobalt(III) complexes of these ligands are reported. Both tetraamine ligands yield exclusively trans geometry upon coordination to the cobalt(III) ion.

• BMB Reports
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• v.52 no.3
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• pp.202-207
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• 2019

Keloids are the most common pathological form of trauma healing, with features that seriously affect appearance and body function, are difficult to treat and have a high recurrence rate. Emerging evidence suggests that miRNAs are involved in a variety of pathological processes and play an important role in the process of fibrosis. In this study, we investigated the function and regulatory network of miR-152-5p in keloids. The miRNA miR-152-5p is frequently downregulated in keloid tissue and primary cells compared to normal skin tissue and fibroblasts. In addition, the downregulation of miR-152-5p is significantly associated with the proliferation, migration and apoptosis of keloid cells. Overexpression of miR-152-5p significantly inhibits the progression of fibrosis in keloids. Smad3 is a direct target of miR-152-5p, and knockdown of Smad3 also inhibits fibrosis progression, consistent with the overexpression of miR-152-5p. The interaction between miR-152-5p and Smad3 occurs through the Erk1/2 and Akt pathways and regulates collagen3 production. In summary, our study demonstrates that miR-152-5p/Smad3 regulatory pathways involved in fibrotic progression may be a potential therapeutic target of keloids.

• Korean Journal of Clinical Laboratory Science
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• v.54 no.3
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• pp.201-208
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• 2022

Neuroinflammation is defined as a neurological inflammation within the brain and the spinal cord. In neuroinflammation, microglia are the tissue-resident macrophages of the central nervous system, which act as the first line of defense against harmful pathogens. Dexmedetomidine (Dex) has an anti-inflammatory effect in many neurological conditions. Additionally, the microRNA-30a-5p (miR-30a-5p) mimic has been proven to be effective in macrophages in inflammatory conditions. This study aimed to investigate the synergistic anti-inflammatory effects of both miR-30a-5p and Dex in lipopolysaccharide (LPS)-induced BV2 cells. This study showed that miR-30a-5p and Dex decreased nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) translocation in LPS-induced BV2 cells. MiR-30a-5p and Dex alleviated tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), LPS-induced phosphorylation c-Jun N-terminal kinases (JNK), extracellular signal-regulated kinase (ERK) and p38. Also, the expression of the NOD-like receptor pyrin domain containing 3 inflammasome (NLRP3), cleaved caspase-1, and ASC was inhibited. Furthermore, LPS-stimulated nitric oxide (NO) production, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) expression were attenuated by Dex and miR-30a-5p. Our results indicate that a combination of Dex and miR-30a-5p, attenuates NF-κB activation, the mitogen-activated protein kinase (MAPK) signaling pathway, and inflammatory mediators involved in LPS-induced inflammation and inhibits the activation of the NLRP3 inflammasome in LPS-activated BV2 cells.

• Biomolecules & Therapeutics
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• v.31 no.2
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• pp.176-182
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• 2023

Among 14 subtypes of serotonin receptors (5-HTRs), 5-HT_2AR plays important roles in drug addiction and various psychiatric disorders. Agonists for 5-HT_2AR have been classified into three structural groups: phenethylamines, tryptamines, and ergolines. In this study, the structure-activity relationship (SAR) of phenethylamine and tryptamine derivatives for binding 5-HT_2AR was determined. In addition, functional and regulatory evaluation of selected compounds was conducted for extracellular signal-regulated kinases (ERKs) and receptor endocytosis. SAR studies showed that phenethylamines possessed higher affinity to 5-HT_2AR than tryptamines. In phenethylamines, two phenyl groups were attached to the carbon and nitrogen (R³ ) atoms of ethylamine, the backbone of phenethylamines. Alkyl or halogen groups on the phenyl ring attached to the β carbon exerted positive effects on the binding affinity when they were at para positions. Oxygen-containing groups attached to R³ exerted mixed influences depending on the position of their attachment. In tryptamine derivatives, tryptamine group was attached to the β carbon of ethylamine, and ally groups were attached to the nitrogen atom. Oxygen-containing substituents on large ring and alkyl substituents on the small ring of tryptamine groups exerted positive and negative influence on the affinity for 5-HT_2AR, respectively. Ally groups attached to the nitrogen atom of ethylamine exerted negative influences. Functional and regulatory activities of the tested compounds correlated with their affinity for 5-HT_2AR, suggesting their agonistic nature. In conclusion, this study provides information for designing novel ligands for 5-HT_2AR, which can be used to control psychiatric disorders and drug abuse.

• Bulletin of the Korean Chemical Society
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• v.18 no.3
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• pp.311-316
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• 1997

The synthesis and characterization of the mononuclear group 13 heterocyclic carboxaldehyde methyldithiocarbazate complexes Me2M[NC5H4CRNNC(S)SCH3] (M=Al, R=H(1); M=Ga, R=H(2); M=Al, R=CH3(3); M-Ga, R=CH3(4); M=In, R=CH3(5)) are described. Compounds 1-5 were prepared by the reaction of MMe3 (M=Al, Ga, In) with 2-formy or 2-acetylpyridine-S-methyldithiocarbazate in toluene. These compounds 1-5 have been characterized by microanalysis, NMR (1H, 13C) spectroscopy, mass spectra, and single-crystal X-ray diffraction. X-ray single-crystal diffraction analyses reveal that 4-5 are mononuclear metal compounds with coordination number of 5 and N,N,S coordination mode.

• Journal of Oral Medicine and Pain
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• v.16 no.1
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• pp.73-84
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• 1991

저자는 연령을 추정하기 위한 기본자료를 얻기 위하여 상하악의 대구치, 소구치의 발육정도를 평가하였다. Orthopantomograph를 촬영한 722명의 3,464개 치아를 대상으로 crown-root ratio를 측정하여 발육정도를 평가하였으며, 다음과 같은 결론을 얻었다. 1. 완전히 형성된 치아의 crown-root ration에는 남녀간에 유의한 차이가 없었다. 2. 발육중인 치아의 crown-root ratio에는 좌우측간에 유의한 차이가 없었다. 3. 각 치아의 crown-root ratio를 이용한 연령추정의 회귀방정식은 다음과 같다. 남자: 여자 : 하악좌측 제 2대구치 : Y=4.599X+7.832(r=0.8337) 하악 좌측 제 2대구치 : Y=4.857X+7.429(r=0.8975) 제 1대구치 : Y=5.179X+2.324(r=0.7948) 제 1대구치 : Y=5.919X+2.018(r=0.8144) 제 2소구치 : Y=3.863X+7.432(r=0.8638) 제 2소구치 : Y=3.679X+7.275(r=0.8819) 제 1소구치 : Y=3.472X+7.120(r=0.8352) 제 1소구치 : Y=4.001X+6.544(r=0.9024) 하악우측 제 2대구치 : Y=4.447X+7.938(r=0.8045) 하악 우측 제 2대구치 : Y=4.653X+7.365(r=0.8598) 제 1대구치 : Y=5.954X+1.495(r=0.7777) 제 1대구치 : Y=5.449X+2.012(r=0.7553) 제 2소구치 : Y=3.894X+7.253(r=0.8689) 제 2소구치 : Y=3.772X+7.025(r=0.8719) 제 1소구치 : Y=4.189X+6.717(r=0.8370) 제 1소구치 : Y=4.327X+6.193(r=0.8524) 상악좌측 제 2대구치 : Y=4.430X+7.722(r=0.7538) 상악 좌측 제 2대구치 : Y=4.876X+7.606(r=0.8311) 제 1대구치 : Y=4.645X+2.886(r=0.6894) 제 1대구치 : Y=6.754X+1.891(r=0.5378) 제 2소구치 : Y=4.391X+6.686(r=0.7700) 제 2소구치 : Y=1.245X+10.575(r=0.1908) 제 1소구치 : Y=5.564X+6.037(r=0.9032) 제 1소구치 : - 상악우측 제 2대구치 : Y=4.587X+7.966(r=0.7882) 상악 우측 제 2대구치 : Y=4.454X+7.803(r=0.8443) 제 1대구치 : Y=4.047X+4.124(r=0.6352) 제 1대구치 : Y=6.336X+2.911(r=0.4688) 제 2소구치 : Y=2.920X+8.089(r=0.7277) 제 2소구치 : Y=3.105X+8.082(r=0.6381) 제 1소구치 : Y=3.264X+6.970(r=0.7292) 제 1소구치 : - 4. Orthopantomograph상의 crown-root ratio를 이용한 연령의 추정에는 상악치아들 보다 하악치아들이 더 정확하게 사용될 수 있다.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.18
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• pp.7575-7581
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• 2014

MicroRNAs (miRNAs) play an essential role in the development and progression of nasopharyngeal carcinomas (NPC). Despite advances in the field of cancer molecular biology and biomarker discovery, the development of clinically validated biomarkers for primary NPC has remained elusive. In this study, we investigated the expression and clinical significance of miRNAs as novel primary NPC diagnostic biomarkers. We used an array containing 2, 500 miRNAs to identify 22 significant miRNAs, and these candidate miRNAs were validated using 67 fresh NPC and 25 normal control tissues via quantitative real-time PCR (qRT-PCR). Expression and correlation analyses were performed with various statistical approaches, in addition to logistic regression and receiver operating characteristic curve analyses to evaluate diagnostic efficacy. qRT-PCR revealed five differentially expressed miRNAs (miR-93-5p, miR-135b-5p, miR-205-5p and miR-183-5p) in NPC tissue samples relative to control samples (p<0.05), with miR-135b-5p and miR-205-5p being of significant diagnostic value (p<0.01). Moreover, comparison of NPC patient clinicopathologic data revealed a negative correlation between miR-93-5p and miR-183-5p expression levels and lymph node status (p<0.05). These findings display an altered expression of many miRNAs in NPC tissues, thus providing information pertinent to pathophysiological and diagnostic research. Ultimately, miR-135b-5p and miR-205-5p may be implicated as novel NPC candidate biomarkers, while miR-93-5p, miR-650 and miR-183-5p may find application as relevant clinical pathology and diagnostic candidate biomarkers.

• Mycobiology
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• v.33 no.2
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• pp.109-112
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• 2005

The internal transcribed spacer (ITS) regions including the 3'-end of 18S rRNA gene, 5.8S rRNA gene and the 5'-end of the 28S rRNA gene of Rhizopus spp. were amplified by PCR and analyzed by DNASIS program. Length polymorphism of these region ranged from 564 bp in R. oryzae to 789bp in R. stolonifer. The length and sequence of 5.8S was very conserved with $154{\sim}155\;bp$. The sequence of ITS2 was more variable than that of ITS1. The base substitution rates were ranged from 0 to 0.6069 per site, and higher rate was found in R. stolonifer. In general, transition was usually more frequent than transversion. On the basis of sequencing results, four groups were clustered with value of 61.9% similarity; R. oryzae, R. micros pores, R. homothallicus, and R. stolonifer groups.

• Horticultural Science & Technology
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• v.33 no.6
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• pp.869-876
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• 2015

Wild relative species of domesticated crops are useful genetic resources for improving agronomic traits. Cytogenetic investigations based on chromosome composition provide insight into basic genetic and genomic characteristics of a species that can be exploited in a breeding program. Here, we used FISH analysis to characterize the ploidy level, chromosome constitution, and genomic distribution o f 5S and 4 5S r ibosomal DNA (rDNA) in four wild Cucurbitaceae species, namely, Citrullus lanatus (Thunb.) Mansf. var. citroides L. H. Bailey (2n = 22), Melothria japonica Maxim. (2n = 22), Sicyos angulatus L. (2n = 24), and Trichosanthes kirilowii Maxim. (2n = 66, 88, 110 cytotypes), collected in different areas of Korea. All species were diploids, except for T. kirilowii, which included hexa-, octa-, and decaploid cytotypes (2n = 6x = 66, 8x = 88, and 10x = 110). All species have small metaphase chromosomes in the range of $2-5{\mu}m$. The 45S rDNA signals were localized distally compared to the 5S rDNA. C. lanatus var. citroides and M. japonica showed one and two loci of 45S and 5S rDNA, respectively, with co-localization of rDNA signals in one M. japonica chromosome. S. angulatus showed two co-localized signals of 5S and 45S rDNA loci. The hexaploid T. kirilowii cytotype showed five signals each for 45S and 5S rDNA, with three being co-localized. This is the first report of hexaploid and decaploid cytotypes in T. kirilowii. These results will be useful in future Cucurbitaceae breeding programs.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.24 no.3 s.174
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• pp.794-802
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• 2000

Since the examination of CTOD problem revealed that the discrepancy among CTOD's was a matte., of definition, the relationships among parameters based on different definitions have been studied Particularly, the relationship between $\sigma$BS R-curve based on BS 7448 and R-curve based on the recently introduced $\sigma$5 parameter was investigated in this research. For the comparison, compact tension specimens of used 1Cr-0.5Mo steel, heat treated 1Cr-0.5Mo steel to mimic the new one, A12024-T6, and A12024-T351 were prepared and tested. Consequently, the relationship between $\sigma$5 and $\sigma$BS R-curves for tested materials were established by shifting the rotational center which could determined by rotation factor and ligament size.