• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.13 no.1
• /
• pp.1-14
• /
• 2008

Eight comprehensive oceanographic cruises on a squared $30{\times}30\;km$ area have been made to investigate the short and long-term impacts on the water qualities due to the sand mining operations at Exclusive Economic Zone (EEZ) in the central Yellow Sea from 2004 to 2007. The area was categorized to 'Sand Mining Zone', 'Potentially Affected Zone', and 'Reference Zone'. The investigation covered suspended solids, nutrients (nitrate, nitrite, ammonium, phosphate), and chlorophyll-a in seawater and several parameters such as water temperature, salinity, pH, and ORP. Additionally, several intensive water collections were made to trace the suspended solids and other parameters along the turbid water by sand mining activities. The comprehensive investigation showed that suspended solids, nitrate, chlorophyll-a and ORP be sensitively responding parameters of seawater by sand mining operations. The intensive collection of seawater near the sand mining operation revealed that each parameter show different distribution pattern: suspended solids showed an oval-shaped distribution of the north-south direction of 8 km wide and the east-west direction of 5 km wide at the surface and bottom layers. On the other hand, phosphate showed so narrow distribution not to traceable. Also ammonium showed a limited distribution, but its boundary was connected to the high nitrate and chlorophyll-a concentrations with high N/P ratios. From the last 4 years of the comprehensive and intensive investigations, we found that suspended solids, ammonium, nitrate, chlorophyll-a, and ORP revealed the sensitive parameters of water quality for tracing the sand mining operations in seawater. Especially suspended solids and ORP would be useful tracers for monitoring the water qualities of remote area like EEZ in Yellow Sea.

• Economic and Environmental Geology
• /
• v.57 no.2
• /
• pp.243-251
• /
• 2024

In this study, a surface geological survey was first conducted to investigate aggregate resources in the Yeongam area of Jeollanam-do, and a drilling survey was conducted in the lower part of the surface, which was difficult to identify through a surface geological survey, to determine the spatial distribution of aggregates. Drilling sites were selected considering the topographical development and Quaternary alluvium characteristics of the study area, and river aggregate drilling surveys were conducted at a total of 5 points and land aggregate drilling surveys were conducted at a total of 28 points. Borehole core sediments were classified into seven sedimentary units to determine whether they could be used as aggregates, and optically stimulated luminescence dating was performed on representative boreholes to measure the depositional period for each sedimentary unit. As a result of the study, most of the Yeongam area had a very wide river basin, so it was estimated that there would be a large amount of aggregate, but the amount of aggregate was evaluated to be very small compared to other cities and counties. Most of the unconsolidated sedimentary layers in the Yeongam area are composed of blue-grey marine clay with a vertical thickness of more than 10 m. The sand-gravel layer corresponding to the aggregate section is distributed in the lower part of the marine clay, thinly covering the bedrock weathering zone. This is because the amount of aggregates themselves is small and most of the aggregates are distributed at a depth of 10 m below the surface, which is currently difficult to develop, so the possibility of developing aggregates is evaluated to be very low. As a result of dating, it can be seen that the blue-grey marine clay layer is an intertidal sedimentary layer formed as the sea level rose rapidly about 10,000 years ago. The deposition process continued from 10,000 years ago to the present, and as a result, a very thick clay layer was deposited. This clay layer was formed very dominantly for about 6,000 to 8,000 years, and the sand-gravel layer in the section where aggregates deposited in the Pleistocene period can exist was measured to have been deposited at about 13.0 to 19.0 ka, and about 50 ka, showing that it was deposited as paleo-fluvial deposits before the marine transgression process.

• Research in Plant Disease
• /
• v.29 no.4
• /
• pp.384-389
• /
• 2023

Milling can affect the distribution of mycotoxins in small grains. To investigate the effects on barley, seven hulled barley and three naked barley samples naturally contaminated with trichothecenes and zearalenone were obtained and milled at commonly used rates. Both barleys were simultaneously contaminated with deoxynivalenol and its acetyl derivatives (98.1-2,197.8 ㎍/kg), nivalenol and its acetyl derivative (468.5-3,965.1 ㎍/kg), and zearalenone (4.1-274.2 ㎍/kg). Milling hulled barleys at a rate of 67% reduced the mycotoxins in the grain by 90.9% for deoxynivalenol, 87.7% for nivalenol, and 93.2% for zearalenone. The reduction in naked barleys (milled at a rate of 70%) was slightly lower than in hulled barleys, with 88.6% for deoxynivalenol, 80.2% for nivalenol, and 70.1% for zearalenone. In both barleys, the acetyl derivatives of deoxynivalenol and nivalenol were reduced by 100%. However, barley bran had significantly higher mycotoxin concentrations than the pre-milled grains: bran from hulled barley had a 357% increase in deoxynivalenol, 252% increase in nivalenol, and 169% increase in zearalenone. Similarly, bran from naked barley had a 337% increase in deoxynivalenol, 239% increase in nivalenol, and 554% increase in zearalenone. These results show that mycotoxins present in the outer layers of barley grain can be effectively removed through the milling process. As milling redistributes mycotoxins from the grain into the bran, however, it shows that advance monitoring of barley bran is recommended when using barley bran for human or animal consumption.

• Journal of Sericultural and Entomological Science
• /
• v.23 no.1
• /
• pp.1-31
• /
• 1981

Rootability of the hardwood cuttings of mulberry was related not only histological characteristics but dependent on biochemical properties. In this connection, the characteristics of the hardwood cuttings were histologically observed and the growth substances produced by the cuttings were also identified by means of mung bean bioassay. Amino acid, carbohydrate, nucleic acid contents, and the C/N ratio were also analysed. The results are summarized as follows. 1. There were differences in rootability of cuttings between mulberry species and varieties Among the three mulberry species tested, Morus Lhou Koidz. showed the highest rootability while M. bombycis showed the lowest one. In varietal differences in rootability, it was shown that the varieties could be grouped according to rootability: high varieties(above 80%), medium(41~79%), and low(below 40%). The higher varieties were Kemmochi, Nakamaki, Kosen, and Wusuba roso. 2. The histological characteristic of the hardwood cuttings most closely related to rootability was cell layer arrangement in the sclerenchyma tissue. The lower rootability varieties developed two or three overlapping cell layers in the bark tissue and in the higher rootability varieties they were scattered over the primary cortex. 3. In the higher rootability varieties, there was a positive correlation between the development of root primodia and rootability of the hardwood cuttings. It was also shown that there was a close relationship between the size of primodia and the surface area of the lenticel with rootability of the cuttings. 4. Effect of growth substances extracted from the hardwood cuttings were determined by mung bean bioassay. The higher rootability varieties usually showed higher activities of the growth substances, in contrast the lower rootability varieties showed higher activities of the inhibitory substances. 5. It was evident that the substance separated by paper chromatography was identified as indole acetic acid with $R_f$ value ranging from 0.3 to 0.5. The other substances detected at a $R_f$ value ranging from 0.8 to 1.0 and origin to 0.1 were also responsible for rooting. 6. There exists a quantitatively different distribution of growth substances in a synergistic system in the tissues of cuttings, and the balance between growth and inhibitory substances gives rise to the development of rooting. Particularly, no descent of the substances from winter buds resulted in no rooting of cuttings but these substances were produced a week after planting in a warm environment. 7. It was shown that there were positive correlations between carbohydrate ($r=0.72^*$) and total sugar ($r=0.67^*$) and rootability, respectively, but there were negative correlations between reducing sugars ($r=-0.75^*$) and rootability. 8. High C/N ratio gave rise to high rootability($r=0.67^*$). The latter therefore depended on high amount of carbohydrate rather than nitrogen in the cuttings. 9. The content of RNA and DNA in the cuttings was not changed for upto two weeks after the cuttings were planted. Then an increase in RNA content took place in only the high rootability varieties. 10. There were quantitative and qualitative differences in the compositions of the amino acids between the high rootability varieties and the low rootability varieties. More aspartic acid and cystine were found in the higher rootability varieties than in the low rootability varieties.

• Clinical and Experimental Reproductive Medicine
• /
• v.33 no.1
• /
• pp.25-33
• /
• 2006

Objective: The aim of this study was to investigate whether embryonic stem (ES) cells can be established from isolated blastomeres of mouse embryos. Methods: Blastomeres were separated from mouse (C57Bl/6J) 2- or 4-cell embryos. Isolated blastomeres or whole 4-cell embryos were co-cultured with mitosis-arrested STO feeder cells in DMEM supplemented with recombinant murine leukemia inhibitory factor and ES-qualified fetal bovine serum. After the tentative ES cell lines were maintained from isolated blastomeres or whole embryos, some of them were frozen and the others were sub-cultured continually. Characteristics of tentative ES cell lines as were evaluated for specific genes expressions with immunocytochemistry and RT-PCR. Results: One ES cell line (3.0%) was established from isolated blastomere of 2-cell embryo and one cell line (4.0%) from isolated two blastomeres of 4-cell embryo. And five cell lines (16.7%) were established from whole 4-cell embryos. Both cell lines from isolated blastomere and whole embryo expressed mouse ES cell specific markers such as SSEA-1, Oct-4 and alkaline phosphatase. Marker genes of three germ layers were expressed from embryoid bodies of both cell lines. Conclusion: This study suggests that mouse ES cells could be established from isolated blastomeres, although the efficiency is lower than whole embryos. This animal model could be applied to establishment of autologous human ES cells from biopsied blastomeres of preimplantation embryos in human IVF-ET program.

• The Korean Journal of Petroleum Geology
• /
• v.5 no.1_2 s.6
• /
• pp.9-15
• /
• 1997

The study area is located in the Central Bransfield Basin, Antarctica. To analyze the morphology of seafloor, structure of basement, and seismic stratigraphy of the sedimentary layers, we have acquired, processed, and interpreted the multi-channel seismic data. The northwest-southeastern back-arc extension dramatically changes seafloor morphology, volcanic and fault distribution, and basin structure along the spreading ridges. The northern continental shelf shows a narrow, steep topography. In contrast, the continental shelf or slope in the south, which is connected to the Antarctic Peninsula, has a gentle gradient. Volcanic activities resulted in the formation of large volcanos and basement highs near the spreading center, and small-scale volcanic diapirs on the shelf. A very long, continuous normal fault characterizes the northern shelf, whereas several basinward synthetic faults probably detach into the master fault in the south. Four transfer faults, the northwest-southeastern deep-parallel structures, controlled the complex distributions of the volcanos, normal faults, depocenters, and possibly hydrocarbon provinces in the study area. They have also deformed the basement structure and depositional pattern. Even though the Bransfield Basin was believed to be formed in the Late Cenozoic (about 4 Ma), the hydrocarbon potential may be very high due to thick sediment accumulation, high organic contents, high heat flow resulted from the active tectonics, and adequate traps.

• Korean Journal of Environmental Biology
• /
• v.35 no.2
• /
• pp.198-206
• /
• 2017

From June 2007 to May 2008, seasonal variations of bacterial abundance and heterotrophic nanoflagellate (HNF), together with environmental factors, were investigated at weekly and monthly intervals in Kyeonggi Bay. During the study period, the water temperature and salinity varied from $1.9^{\circ}C{\sim}29.0^{\circ}C$ and 31~35.1 psu, respectively. The concentration of ammonia, nitrate+nitrite, phosphate, and silicate ranged from 0.01 to $3.22{\mu}M$, 2.03 to $15.34{\mu}M$, 0.06 to $1.82{\mu}M$, and 0.03 to $18.3{\mu}M$, respectively. The annual average concentration of Chl. a varied from $0.86{\mu}g\;L^{-1}$ to $37.70{\mu}g\;L^{-1}$; the concentration was twice as much at the surface than at the deeper layers. The abundance of bacteria and HNF ranged from $0.29{\times}10^6$ to $7.62{\times}10^6cells\;mL^{-1}$ and $1.00{\times}10^2$ to $1.26{\times}10^3cells\;mL^{-1}$, respectively. In particular, there were significant correlations between bacteria and HNF abundance (p<0.05), and then the high abundance of HNF was frequently observed with an increase of bacterial abundance in summer (p<0.001). Our results therefore indicate that bacterial abundance in the bay was mainly controlled by resources supplied as organic and inorganic substances from Lake Shihwa due to the daily water exchange after dike construction. Also, the bacterial abundance was significantly controlled by HNF grazing pressure (top-down) in the warm seasons, i.e. excluding winter, in the Kyeonggi Bay.

• Journal of Periodontal and Implant Science
• /
• v.33 no.3
• /
• pp.457-474
• /
• 2003

The ultimate objective of periodontal treatment is to get rid of an on-going periodontal disease and further regenerate the supporting tissue, which is already destroyed, functionally. Currently, the bone grafting operation using various kinds of bone grafting materials and the operation for induced regeneration of periodontal tissue using the blocking membrane are performed for regeneration of the destroyed periodontal tissue. However, there are respective limitations Galenical preparations, which are used for regeneration of periodontal of tissue, has less risk of rejective reaction or toxicity that may be incidental to degradation and their effect is sustainable. Thus, in case they are applicable to a clinic, they can he used economically. Chitosan has such compatibility, biological actions including antibacterial activity, acceleration of wound treatment, etc., and excellent mechanical characteristics, which has recently aroused more interest in it. Also, it has been reported that it promotes osteogenesis directly or indirectly by functioning as a matrix to promote migration and differentiation of a specific precussor cell (for example, osteoblast) and further inhibiting the function of such a cell as fibroblast to prevent osteogenesis. In this study, the pure chitosan solution, which was obtained by purifying chitosan, was used. However, since this chitosan is of a liquiform, it is difficult to sustain it in a defective region. It is, therefore, essential to use a carrier for delivering chitosan to, and sustaining it gradually in the defective region. In the calvarial defect model of the Sprague-Dawley rat, it is relatively easy to maintain a space. Therefore, in this study, the chitosan solution with which ACS was wetted was grafted onto the defective region, For an experimental model, a calvarial defect of rat m s selected, and a critical size of the defective region was a circular defect with a diameter of 8 mm. A group in which no treatment was conducted for the calvarial defect was set as a negative control group. Another group in which treatment was conducted with ACS only was set as a positive control group (ACS group). And another group in which treatment was conducted was conducted with by grafting the pure chitosan solution onto the defective region through ACS which was wetted with the chitosan solution was set an experimental group (Chitosan/ACS group). Chitosan was applied to the Sprague-Dawley rat's calvarial bone by applying ACS which was wetted with the chitosan solution, and each Sprague-Dawley rat was sacrificed respectively 2 weeks and 8 weeks after the operation for such application. Then, the treatment results were compared and observed histologically and his tometrically. Thereby, the following conclusions were obtained. 1. In the experimental group, a pattern was shown that from 2 weeks after the operation, vascular proliferation proceeded and osteogenesis proceeded through osteoblast infiltration, and at 8 week after the operation, ACS was almost absorbed, the amount of osteogensis was increased and many osteoid tissue layers were observed. 2. At 2 weeks after the operation, each amount of osteogenesis appeared to be 8.70.8 %, 13.62.3 % and 4.80.7 % respectively in the experimental group, the positive control group and the negative control group. Accordingly, it appeared to be higher in the Experimental group and the positive control group than in the negative control group, but there was no significant difference statistically (p<0.01). 3. At 8 weeks after the operation, each amount of osteogenesis appeared to be 62.26.1%, 17.42.5 % and 8.21.4 % respectively in the experimental group, the positive control group and the negative control group. Accordingly, it appeared to be substantially higher in the experimental group than in the positive control group and the negative control group, and there was a significant difference statistically (p<0.01). As a result of conducting the experiment, when ACS was used as a carrier for chitosan, chitosan showed effective osteogenesis in the perforated defective region of the Sprague-Dawley rat's calvarial bone.

• Korean Journal of Ecology and Environment
• /
• v.44 no.3
• /
• pp.303-313
• /
• 2011

Field observations and culture experiments have been carried out during the rainy season (on the 6th, 8th and 27th July 2009) to examine changes in the primary productivity and associated plant pigments in the estuary of the Seom-jin River. Primary productivity was determined at four sampling stations along the salinity gradient. On 6th July (before heavy rain) primary productivity ranged from 689~1,169 mgC $m^{-2}$ $d^{-1}$. On the 8th, just after more than 216.5 mm of precipitation, euphotic layers at all stations were reduced to very shallow water because of the high concentration of suspended solids in the water. This resulted in dramatically decreased primary productivity down to as low as 12~32 mg C $m^{-2}$ $d^{-1}$. However, after the rain, primary productivity on the 27th ranged from 266~999 mgC $m^{-2}$ $d^{-1}$, demonstrating a fast recovery in the upper stream water to similar productivity levels to those before the rainy season. Concentration of fucoxanthin in the water was highest on the 6th July. Before the rain, concentration of the zeaxanthin, increased as the salinity decreased. Immediately after the heavy rain, the Chl b (Chlorophytes) concentration was higher at all sites than before the rainy season. The concentration of fucoxanthin decreased after the heavy rain. At the downstream site, peridinin (Dinoflagellates) were found. During the rainy season, the diatoms contributed to the primary productivity at all sites. However, after the rainy season, Chl b (Chlorophytes) and Peridinin (Dinoflagellates) increased, demonstrating the enhanced contribution of those species in addition to diatoms.

• Korean Journal of Poultry Science
• /
• v.37 no.3
• /
• pp.237-245
• /
• 2010

A survey of transovarially transmitted diseases, including salmonellosis [(pullorum disease; PD)/(fowl typhoid; FT)], mycoplasmosis, avian infectious anemia (CIA), and fowl adenovirus infection was conducted in the breeder chickens from August to December in 2009. The numbers of flocks sampled out were: Grand Parents Stock (GPS), 45; Parents Stock (PS) 1,018, Baeksemi breeder (BSB) 54. The seroprevalence of salmonellosis (PD/FT) were 0% (GPS), 3.2% (PS), and 3% (BSB), respectively. A total of 983 chicken farms were affected with FT outbreaks between 2000 and 2008. The incidence of FT in commercial broilers, Baeksemi, commercial layers, native chickens, and broiler breeders was 44.3%, 26.2%, 15.7%, 12.6% and 1.08%, respectively. Of the affected broilers, over 90% birds were under 2 weeks of age, indicating it was possible that they were infected with S. gallinarum via vertical transmission. The sero-positive flocks against Mycoplasma gallisepticum (MG) were 71.1% (GPS), 88.7% (PS), 88.7% (BSB), while the rates of positive flocks against Mycoplasma synoviae (MS) were 86.0% (GPS), 77.0% (PS), and 98.0% (BSB). In GP and parent farms, the detection rates on specific genes of CIA virus were 19/45 (42.2%), and 169/1039 (18.0%), respectively, whereas the seroprevalence of CIA were 86.0% in GPS and 93.7% in PS flocks. In addition, positive flocks of fowl adenoviruses were 4.4% (GPS), 2.7% (PS) and 9.35% (BSB), respectively. As the results, avian mycoplasmosis and CIA have been more prevailing in chicken breeder than avian salmonellosis and fowl adenovirus infection in Korea.