• 한국축산식품학회지
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• 제37권5호
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• pp.646-653
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• 2017

Alcalase hydrolysis of liquid egg white was used to produce 5-hydroxytryptophan (HTP) under various conditions and investigate the sleep-potentiating activity of liquid egg white hydrolysate (LEH) on pentobarbital-induced sleep. Alcalase hydrolysis yielded the highest content of 5-HTP ($13.50{\mu}g/mL$), while neutrase hydrolysis showed the lowest 5-HTP content ($5.23{\mu}g/mL$). The liquid egg white to water ratio (1:1) was optimal for the production of 5-HTP with high amino-nitrogen (A-N) content and degree of hydrolysis. The 5-HTP, amino-nitrogen, and degree of hydrolysis increased until 24 h of hydrolysis and slightly increased thereafter during hydrolysis with 2% and 5% enzyme addition. 5-HTP administration at doses of 6 and 9 mg/kg significantly increased sleep duration and decreased sleep latency time compared to that in the control (p<0.05). LEH (150 mg/mouse), which was equivalent to 5-HTP at 6 mg/kg, significantly decreased sleep latency time and increased sleep duration time compared to that in the control (p<0.05). Oral administration of LEH showed sleep-potentiating effects because of 5-HTP. The sleep-potentiating activity of LEH may have occurred through 5-HTP in our pentobarbital-induced sleep model. LEH may be a valuable alternative to sleep enhancement and may be used as a sleep-potentiating agent.

• Nutrition Research and Practice
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• 제12권3호
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• pp.208-214
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• 2018

BACKGROUND/OBJECTIVES: The aim of this study was to evaluate the biological and sleep-promoting effects of combined ${\gamma}$-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) using caffeine-induced sleepless fruit flies, ICR mice, and Sprague-Dawley rats. MATERIALS/METHODS: Video-tracking analysis was applied to investigate behavioral changes of Drosophila melanogaster. Pentobarbital-induced sleep test and electroencephalogram (EEG) patterns were used for analysis of sleep latency, duration, and quantity and quality of sleep in vertebrate models. RESULTS: Administration of combined GABA/5-HTP could significantly reverse the caffeine induced total distance of flies (P < 0.001). Also, individually administered and combined GABA/5-HTP significantly increased the total sleeping time in the caffeine-induced sleepless ICR mice (P < 0.001). In the caffeine-induced sleepless SD-rats, combined GABA/5-HTP showed significant differences in sleep quality between individual amino acid administrations (P < 0.05). CONCLUSIONS: Taken together, we identified inhibitory effects of combined GABA/5-HTP in locomotor activity, sleep quantity and quality in caffeine-induced sleepless models, indicating that combined GABA/5-HTP may be effective in patients with insomnia by providing sufficient sleep.

• 생약학회지
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• 제26권4호
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• pp.355-359
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• 1995

To find serotonin(5-hydroxytryptamine, 5-HT)-antagonizing activities in traditional herbal drugs, crude extracts from 66 kinds of traditional herbal drugs were randomly screened for inhibitory effects on 5-hydroxytryptophan(HTP)-induced diarrhea in mice. Intraperitoneal injection of 5-HTP(2.5 mg/kg) induced diarrhea in 92% of mice, when observed from 10 to 15 min after injection. Crude extracts(2 g/kg) from 66 kinds of traditional herbal drugs were orally pretreated for 1 h before 5-HTP injection. Of the 66 herbal drugs screened, Ephedrae Herba(麻黃), Cimicifugae Rhizoma(升麻), Anisi stellati Fructus(八角茴香), Aurantii Fructus(枳實), Polygalae Radix(遠志) showed the most potent inhibiting activities against 5-HTP(2.5 mg/kg)-induced diarrhea in mice. There are at least 3 possible mechanisms that would be responsible for the inhibitory effect of crude extracts on 5-HTP-induced diarrhea; 1) crude extract-induced inhibition of the activity of aromatic aminoacid decarboxylase catalyzing the conversion of 5-HTP to 5-HT, 2) crude extract-induced blockade of 5-HT receptor(s) in the gastrointestinal tract responsible for 5-HTP-induced diarrhea, 3) crude extract-induced inhibition of gastrointestinal activity, irrespective of 5-HT system. The exact mechanisms and molecules, responsible for the inhibitory effect of crude extracts on 5-HTP-induced diarrhea remain to be clarified.

• Biomolecules & Therapeutics
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• 제31권4호
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• pp.402-410
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• 2023

Long-term administration of levodopa (L-DOPA) to patients with Parkinson's disease (PD) commonly results in involuntary dyskinetic movements, as is known for L-DOPA-induced dyskinesia (LID). 5-Hydroxytryptophan (5-HTP) has recently been shown to alleviate LID; however, no biochemical alterations to aberrant excitatory conditions have been revealed yet. In the present study, we aimed to confirm its anti-dyskinetic effect and to discover the unknown molecular mechanisms of action of 5-HTP in LID. We made an LID-induced mouse model through chronic L-DOPA treatment to 6-hydroxydopamine-induced hemi-parkinsonian mice and then administered 5-HTP 60 mg/kg for 15 days orally to LID-induced mice. In addition, we performed behavioral tests and analyzed the histological alterations in the lesioned part of the striatum (ST). Our results showed that 5-HTP significantly suppressed all types of dyskinetic movements (axial, limb, orolingual and locomotive) and its effects were similar to those of amantadine, the only approved drug by Food and Drug Administration. Moreover, 5-HTP did not affect the efficacy of L-DOPA on PD motor manifestations. From a molecular perspective, 5-HTP treatment significantly decreased phosphorylated CREB and ΔFosB expression, commonly known as downstream factors, increased in LID conditions. Furthermore, we found that the effects of 5-HTP were not mediated by dopamine1 receptor (D1)/DARPP32/ERK signaling, but regulated by AKT/mTOR/S6K signaling, which showed different mechanisms with amantadine in the denervated ST. Taken together, 5-HTP alleviates LID by regulating the hyperactivated striatal AKT/mTOR/S6K and CREB/ΔFosB signaling.

• 한국식품과학회지
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• 제47권1호
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• pp.75-80
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• 2015

기능성 소재로써 어성초의 활용 가능성을 검토하고 제빵시 최적 첨가 비율을 결정하기 위하여, 어성초 분말을 밀가루 대신 0.3, 0.5, 1.0%의 비율로 첨가하여 식빵을 제조하면서 어성초 분말이 제빵 특성 및 품질에 미치는 영향을 검토하였다. 어성초 분말의 조회분 9.98%, 조지방 7.50%, 조단백질 17.79%를 나타내었고 어성초 에탄올 추출물의 총 폴리페놀 함량은 15.44 mg/mL이었으며, DPPH 라디칼의 소거능은 77.61%, 아질산염 소거능은 15.38%를 나타내었다. 어성초 분말을 첨가한 반죽의 pH는 5.08-5.14로 대조구(pH 5.16)에 비해 다소 낮았다. 식빵의 pH는 5.18-5.21로 모든 처리구가 반죽의 pH보다 높았으나, 시료간의 유의적인 차이는 나타나지 않았다. 어성초 분말을 첨가한 반죽의 발효능은 대조구와 동일하였고, 어성초 분말 첨가량이 증가할수록 굽기 손실률은 다소 증가하였으나, 유의적 차이는 나타나지 않았다. 식빵의 무게가 다소 감소하였으나, 1% 범위 내의 첨가에 의한 식빵 무게의 유의적 차이는 없었다. 식빵의 부피는 어성초 분말 첨가량이 증가할수록 유의적으로 감소하였으며(p<0.05), 비용적도 감소하였다. 빵반죽, 빵의 표면과 내부의 색도는 어성초 분말 첨가구가 대조구에 비해 $L^*$값과 $a^*$값은 유의적으로 낮았다. 빵반죽과 빵 내부의 $b^*$값은 어성초 분말 첨가구가 대조구보다 높았다. 어성초 분말 첨가 농도에 의존적으로 식빵의 DPPH 라디칼 소거능은 유의적으로 증가하였고(p<0.05). 총 폴리페놀 함량도 어성초 분말 첨가 식빵이 $806.18-1092.10{\mu}g/mL$으로 대조구의 $731.47{\mu}g/mL$보다 높았다. 맛, 풍미와 씹힘성에 대한 기호도는 대조구에 비해 어성초 분말 첨가 식빵이 낮게 평가되었으나 각 시료별 유의적 차이는 나타내지 않았고. 색은 어성초 분말 첨가 식빵이 대조구보다 높았다. 종합적 기호도는 HTP-0.5가 3.78가 가장 높았으며, HTP-1.0가 3.00으로 가장 낮았다.

• Molecules and Cells
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• 제23권3호
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• pp.357-362
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• 2007

There is an increasing demand for high throughput (HTP) methods for gene analysis on a genome-wide scale. However, the current repertoire of HTP detection methodologies allows only a limited range of cellular phenotypes to be studied. We have constructed two HTP-optimized expression vectors generated from the red fluorescent reporter protein (RFP) gene. These vectors produce RFP-tagged target proteins in a multiple expression system using gateway cloning technology (GCT). The RFP tag was fused with the cloned genes, thereby allowing us localize the expressed proteins in mammalian cells. The effectiveness of the vectors was evaluated using an HTP-screening system. Sixty representative human C2 domains were tagged with RFP and overexpressed in HiB5 neuronal progenitor cells, and we studied in detail two C2 domains that promoted the neuronal differentiation of HiB5 cells. Our results show that the two vectors developed in this study are useful for functional gene analysis using an HTP-screening system on a genome-wide scale.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1993년도 제2회 신약개발 연구발표회 초록집
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• pp.78-78
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• 1993

1) 측좌핵파괴 후 muricide를 비롯하여 startle, struggle등의 정동행동의 변화가 뇌손상 초기에는 현저히 발현되나 muricide를 제외하고 기간경과와 함께 점차 감소한다. 2) 봉선핵파괴 동물은 muricide, moose-eating behavior 등의 극심한 정동행동의 변화를 유발한다. 3) 측자핵파괴로 유발된 muricide는 1-DOPA, nomifensine, desipramine 등에 의하여 현저히 억제되었으나 HPD 에는 감수성이 낮았다. 봉선핵파괴로 유발된 muricide는 L-5-HTP, 4) 봉선핵파괴로 유발된 muricide는 L-5-HTP, clomipramine, nomifensine 등에 의하여 현저하게 억제되었다.

• 한국조리학회지
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• 제23권1호
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• pp.28-36
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• 2017

This study made macaroon with the addition of Helianthus tuberosus L. powder so as to increase the application of Helianthus tuberosus L. powder (HTP) to food products and look into the quality characteristics depending on an addition ratio. Helianthus tuberosus L. powder 0%, 0.5%, 1.0%, 1.5%, and 2.0% were added, respectively, to make macaroon. As Helianthus tuberosus L. powder was added more, the moisture content tended to decrease more significantly (p<0.001). As Helianthus tuberosus L. powder was added more, lightness and yellowness tended to reduce more significantly (p<0.001). As Helianthus tuberosus L. powder was added more, the content of total phenol compounds and DPPH radical scavenging activity were significantly increased (p<0.001). In terms of texture analysis, hardness, gumminess, cohesiveness and chewiness were significantly increased, as the Helianthus tuberosus L. powder was increased (p<0.05). In terms of sensory test, regarding taste, 0.5% HTP group had the highest points. And as color, flavor, and texture were improved, 1.5% HTP showed the most desirable result in terms of overall acceptability. Results, if Helianthus tuberosus L. powder is added for cooking macaroon, it seems to increase anti-oxidation function and develop functional macaroon with excellent sensory factors.

• 한국동물학회지
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• 제8권2호
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• pp.85-88
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• 1965

There are few papers on the biosynthesis of serotonin in the enterochromaffin cell in the pancrease. The present studies were performed to clarify this problem by use of autoradiography in the auinea pig injected of 5-HTP-H3 in dose of 1 vc/g. body weight, The appearance of the above mentioned cells in the pancreas of the rabbit was also studied morphologically. The results are as follows ; 1. Enterochromaffin cells were found in the epithelia of all the pancreating ducts and a few acini of normal male rabbit and most numerous in the intramural pancreatic ducts. 2. The redioautographic silver grains overling the enterochormaffin cells in the guinea pig pancreas are considered to be due to the newly synthesized radioactive serotonin in the cells from injected 5-HTP-H3.

• Archives of Pharmacal Research
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• 제9권3호
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• pp.169-174
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• 1986

Bilateral lesion of nucleus accumbens septi (N, AB), one of the mesolimbic nuclei, resulted in hyperirritability and muricide including mouse eating behavior in rats. The effects of various drugs on hyperirritability and muricide induced by NAB lesion were investigated in rats. Hyperirritability in NAB rats were significantly reduced by L-DOPA L-5-HTP major and minor tranquilizers but not reduced by MA, ATP and imipriamine-like antidepressants. On the other hand, muricide in NAB rats was significantly suppressed by L-DOPA, L-5-HTP, major and minor tranquilizers, furthermore, selectively suppressed by MA, ATP and antidepressants. These results suggested that the neural mechanism for inducing muricide is distinct from for hyperirritability in NAB rats, and that muricide in NAB rats is resulted from the increasing of cholinergic activity and reduction of dopaminergic and serotonergic activity.