• Archives of Pharmacal Research
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• v.29 no.2
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• pp.135-139
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• 2006

Activity-guided fractionation of the ethanol extract of the whole plant from Solanum lyratum resulted in the isolation of a new pregnane derivative glycoside, 16-dehydropregnenolone 3-O-${\alpha}$-L-rhamnopyranosyl-($1{\to}2$)-${\beta}$-D-glucopyranosid uronic acid (2), as well as other six known compounds: 16-dehydropregnenolone (1), allopregenolone (3), protocatechuic acid (4), vanillic acid (5), caffeic acid (6), and scopoletin (7). The structures of the isolated compounds were elucidated on the basis of their spectral data and chemical evidences. Compounds 1, 3, 4 were isolated for the first time from this plant. Cytotoxic activities of the isolated compounds were evaluated. Compound 1 exhibited significant cytotoxic activity against A375-S2, HeLa, SGC-7901, and Bel-7402 with $IC_{50}$ values of $13.1{\pm}0.9,\;21.5{\pm}1.0,\;40.2{\pm}0.7$, and $49.8{\pm}1.2\;{\mu}g/mL$, respectively.

• Ocean Systems Engineering
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• v.5 no.2
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• pp.77-89
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• 2015

As the exploitation of oil and gas resources advances into deeper waters and harsher environments, the design and analysis of the flexible risers has become the research focus in the offshore engineering filed. Due to the complexity of the components and the sliding between the adjacent layers, the bending response of the flexible risers is highly non-linear. This paper presents the finite element analysis of the flexible risers under bending loads. The detailed finite element model of the flexible riser is established in ABAQUS software. This finite element model incorporates all the fine details of the riser to accurately predict its nonlinear structural behavior. Based on the finite element model, the bending moment-curvature relationships of a flexible riser under various axisymmetric loads have been investigated. The results have been compared with the analytical ones obtained from the literature and good agreements have been found. Moreover, the stress of the tendon armors has been studied. The non-linear relationship between the armor tendons' stress and the bending loads has been obtained.

• Archives of Pharmacal Research
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• v.24 no.4
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• pp.323-326
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• 2001

Previous studies have demonstrated that CW252053, a quinazoline antifolate, exhibits potent inhibitory activity against thymidylate synthase (TS) as well as cytotoxic activity against tumor cell lines in vitro. In this studys, we evaluated the in vivo antitumor efficacy of CW252053 in the mouse tumor model. Female B6D2F$_1$ mice were injected with LY3.7. 2C TK-/- (thymidine kinase deficient mouse Iymphoma) cells into the gastrocnemius muscle. Then, CW252053 was administered twice daily by intraperitoneal injection for 10 days, and tumor growth was monitored daily by leg diameter measurement. All animals in the vehicle, 5-FU, and low dose (30mgmg/kg CW252053 treated groups died between days 12 and 23 because of the tumor burden. In contrast, dosing with 60 mg/kg of CW252053 produced a cure rat against tumor growth of 37.5% and a survival rate of 50%. Even more significantly, a higher dose of CW252053 (120 mg/kg) elicited both a 100% cure rate and a 100% survival rate at the termination of the study, confirming that this compound has very potent in vivo antitumor activity against tumor growth. During the experimental period of this study no signs of toxicity were observed even at the high CW252053 dosage rate of 120 mg/kg.

• Parasites, Hosts and Diseases
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• v.57 no.5
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• pp.537-541
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• 2019

Toxoplasma gondii infection induces parasite infiltration and apoptosis in the spleen. However, dose-dependent parasite infiltration, apoptosis, body weight alternations and survival in mice remain largely unknown. In this study, mice were intraperitoneally infected with 10, 30 or 100 tachyzoites of T. gondii, respectively. Parasite infiltration and apoptosis in the spleen were analyzed on days 3, 7, and 9 post-infection by immunohistochemistry and flow cytometry. Significantly higher levels of T. gondii infiltration and apoptosis in the spleen were found in 30 and 100 tachyzoites infected mice compared to 10 tachyzoites infected mice on days 7 and 9 post-infection. Although 30 and 100 tachyzoites infected mice showed significant body weight loss compared to 10 tachyzoites infected mice, all of the 100, 30, and 10 tachyzoites infected mice died by days 12, 15, and 17, each respectively. Interestingly, T. gondii infiltration in 10 tachyzoites infected mice were limited to capsule area of the spleen on day 9 post-infection. Several areas of parasite infiltrations were found in the 30 tachyzoites infected mice, where noticeable levels of splenic capsule de-adhesion occurred. These results indicated that parasite infiltration and apoptosis in the spleen, as well as body weight loss (survival) are closely correlated with infection dosage. The level of T. gondii infiltration and apoptosis in the spleen and splenic de-adhesion were dependent on the parasite dose.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.5 no.1
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• pp.45-59
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• 1992

I have been studied the tinnitus. The results are summarized as follows. 1. The etiologies of tinnitus is classified the Zang fa endogenous factors of the Jong-Maek-Hae and exogenous factors of the Oun-Gi, in the Nei Ching. 2. In the endogenous factors the etiologies of the Zang fa is mostly hased deficiency of the kidney, which is concerned with Sim-Hae Gan-Darn-Hae and Bi-Wae-Hae, the etiologies of the phlegm fire is fire is divided into Sin-Hae, Om-Ju-Hu-Mi and No-Gi-Oaek-Sang. 3. The etiologies of the Jong-Maek-Hae is divided into deficiency of the stomach xu of both gi am blood and xu of the kidney. 4. In Nei ching,the etiologies of Oun-Gi divided into Gul-Eum-Pung-Mok and So-Yang-Sang-Hwa of the exgeous factors is regarded to wind and fire as following generations is regrded to wind the endogenous factors caused Sin-Hae Gi-Hae. 5. In the Nei ching, Since the O-Mi-Bo-Sa-Bub is uttered main treated of tinnitus is friquently used Bo-Sin Young-Sim-Sun-Gi and Choung-Gan-Sul- You1 as Zang-Fu Choung-Dam-Gang-Hwa as the Phlegm fire Bo-bi-Sin as the Jong Maek Hae and Gye-Pung-San-Hwa as the Oun-Gi.

• Earthquakes and Structures
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• v.16 no.5
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• pp.513-522
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• 2019

A long-span transmission tower-line system is indispensable for long-distance electricity transmission across a large river or valley; hence, the failure of this system, especially the collapse of the supporting towers, has serious impacts on power grids. To ensure the safety and reliability of transmission systems, this study experimentally and numerically investigates the collapse failure of a 220 kV long-span transmission tower-line system subjected to severe earthquakes. A 1:20 scale model of a transmission tower-line system is constructed in this research, and shaking table tests are carried out. Furthermore, numerical studies are conducted in ABAQUS by using the Tian-Ma-Qu material model, the results of which are compared with the experimental findings. Good agreement is found between the experimental and numerical results, showing that the numerical simulation based on the Tian-Ma-Qu material model is able to predict the weak points and collapse process of the long-span transmission tower-line system. The failure of diagonal members at weak points constitutes the collapse-inducing factor, and the ultimate capacity and weakest segment vary with different seismic wave excitations. This research can further enrich the database for the seismic performance of long-span transmission tower-line systems.

• Journal of Broadcast Engineering
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• v.27 no.5
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• pp.676-684
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• 2022

In this paper, we consider the potential of a metaverse space that can be expanded infinitely through the reinvention of the metaverse space. In particular, this study attempted to establish a new concept of metaverse space, and to present research models and strategies between technologies and humanities. To perform this, this study revealed problems that can be overlooked through characteristic analysis of the metaverse space and proposed ways to overcome them through in-depth interviews with experts. In this way, interdisciplinary research will contribute to the revitalization of the metaverse content industry in the mid to long term. Therefore, through this study, it is expected that reinterpreted metaverse space can reach a practical realization as a meaningful place of life and a creative space through Yi-Fu Tuan's concept.

• Structural Engineering and Mechanics
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• v.81 no.5
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• pp.591-606
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• 2022

When the friction pendulum system and shear keys work together to resist the ground motion, which inclined inputs (non 45°) to the bridge structure, the shear keys in XY direction will be sheared asynchronously, endowed the friction pendulum system with a violent curvilinear motion on the sliding surface during earthquakes. In view of this situation, firstly, this paper abandons the equivalent linearization model of friction and constructs a Spring-Coulomb friction plane isolation system with XY shear keys, and then makes a detailed mechanical analysis of the movement process of friction pendulum system, next, this paper establishes the mathematical model of structural time history response calculation by using the step-by-step integration method, finally, it compiles the corresponding computer program to realize the numerical calculation. The results show that the calculation method in this paper takes advantage of the characteristic that the friction force is always µmg, and creatively uses the "circle making method" to express the change process of the friction force and resultant force of the friction pendulum system in any calculation time step, which can effectively solve the temporal nonlinear action of the plane friction; Compared with the response obtained by the calculation method in this paper, the peak values of acceleration response and displacement response calculated by the unidirectional calculation model, which used in the traditional research of the friction pendulum system, are smaller, so the unidirectional calculation model is not safe.

• BMB Reports
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• v.56 no.8
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• pp.451-456
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• 2023

Deubiquitinases (DUBs) are an essential component of the ubiquitin-proteasome system (UPS). They trim ubiquitin from substrate proteins, thereby preventing them from degradation, and modulate different cellular processes. Ubiquitin-specific protease 14 (USP14) is a DUB that has mainly been studied for its role in tumorigenesis in several cancers. In the present study, we found that the protein levels of USP14 were remarkably higher in gastric cancer tissues than in the adjacent normal tissues. We also demonstrated that the inhibition of USP14 activity using IU1 (an USP14 inhibitor) or the inhibition of USP14 expression using USP14-specific siRNA markedly reduced the viability of gastric cancer cells and suppressed their migratory and invasive abilities. The reduction in gastric cancer cell proliferation due to the inhibition of USP14 activity was a result of the increase in the degree of apoptosis, as evidenced by the increased expression levels of cleaved caspase-3 and cleaved PARP. Furthermore, an experiment using the USP14 inhibitor IU1 revealed that the inhibition of USP14 activity suppressed 5-fluorouracil (5-FU) resistance in GC cells. Collectively, these findings indicate that USP14 plays critical roles in gastric cancer progression and suggest its potential to serve as a novel therapeutic target for gastric cancer treatment.

• 대한상한금궤의학회지
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• v.15 no.1
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• pp.53-84
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• 2023

Objective : The purpose of this study is to explore the therapeutic potential of Shanghanlun for neuropsychiatric disorders through a comparative review of five neuropsychiatric disorder cases diagnosed with Greater yin disease pattern/syndrome (太陰病). Methods : We collected and compared five neuropsychiatric cases diagnosed and treated with Greater yin disease pattern/syndrome (太陰病) from four korean medicine clinics. Clinical symptoms common to the five cases were analyzed by connecting them with Shanghanlun provision 273 and 279. Results : In all five cases, the improvement of chief complaints and decrease in general anxiety were observed after administration of Gyejigajakyak-tang (桂枝加芍薬湯) and Gyejigadaehwang-tang (圭支加大黃湯). In addition, neuropsychiatric symptoms and physical symptoms that are mentioned in Shanghanlun provision 273 and 279 were confirmed in all five cases. Conclusions : We not only reconfirmed that the core features of Greater yin disease pattern/syndrome(太陰病) proposed in previous studies; zi-tong (自痛), yin-er (因爾), jie-ying (結硬), but also that the physical symptoms associated with fu-man (腹満), tu (吐) and zi-li (自利) were common in the presenting symptoms and past histories of the five patients.