• 원자력산업
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• 제5권6호통권28호
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• pp.31-33
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• 1985

• 한국주조공학회지
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• 제25권5호
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• pp.181-189
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• 2005

• Journal of Welding and Joining
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• 제18권5호
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• pp.3-12
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• 2000

• Bulletin of the Korean Chemical Society
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• 제28권12호
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• pp.2459-2465
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• 2007

V2O5/TiO2-ZrO2 catalyst modified with V2O5 was prepared by adding Ti(OH)4-Zr(OH)4 powder into an aqueous solution of ammonium metavanadate followed by drying and calcining at high temperatures. The characterization of prepared catalysts was performed using XRD, DSC, solid-state 51V NMR, and FTIR. In the case of calcination temperature of 500 oC, for the catalysts containing low loading V2O5 below 25 wt % vanadium oxide was in a highly dispersed state, while for catalysts containing high loading V2O5 equal to or above 25 wt % vanadium oxide was well crystallized due to the V2O5 loading exceeding the formation of monolayer on the surface of TiO2-ZrO2. The strong acid sites were formed through the bonding between dispersed V2O5 and TiO2-ZrO2. The larger the dispersed V2O5 amount, the higher both the acidity and catalytic activities for acid catalysis.

• BMB Reports
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• 제53권5호
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• pp.266-271
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• 2020

Breast cancer encompasses a major portion of human cancers and must be carefully monitored for appropriate diagnoses and treatments. Among the many types of breast cancers, triple negative breast cancer (TNBC) has the worst prognosis and the least cases reported. To gain a better understanding and a more decisive precursor for TNBC, two major histone modifications, an activating modification H3K4me3 and a repressive modification H3K27me3, were analyzed using data from normal breast cell lines against TNBC cell lines. The combination of these two histone markers on the gene promoter regions showed a great correlation with gene expression. A list of signature genes was defined as active (highly enriched H3K4me3), including NOVA1, NAT8L, and MMP16, and repressive genes (highly enriched H3K27me3), IRX2 and ADRB2, according to the distribution of these histone modifications on the promoter regions. To further enhance the investigation, potential candidates were also compared with other types of breast cancer to identify signs specific to TNBC. RNA-seq data was implemented to confirm and verify gene regulation governed by the histone modifications. Combinations of the biomarkers based on H3K4me3 and H3K27me3 showed the diagnostic value AUC 93.28% with P-value of 1.16e-226. The results of this study suggest that histone modification analysis of opposing histone modifications may be valuable toward developing biomarkers and targets for TNBC.

• 한국멀티미디어학회논문지
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• 제9권7호
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• pp.864-871
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• 2006

디지털 방송의 발달과 인터넷의 보급으로 디지털 데이터에 대한 요구가 급격히 증가하고 있다. 이러한 디지털데이터의 저작권 보호를 위하여 최근 디지털 워터마킹에 관한 많은 연구가 이루어지고 있다. 본 논문에서 움직임 벡터의 변경을 최소화한 블라인드 비디오 워터마킹 기법을 제안한다. 기존의 움직임벡터 기반의 워터마킹 기법은 움직임벡터의 변경을 이용해 워터마크 삽입을 수행한다. 그러나 움직임벡터의 변경은 영상의 화질저하를 초래한다. 따라서 본 논문에서는 워터마크 정보의 삽입에 따른 움직임벡터의 변경을 최소화할 수 있는 삽입조건을 설정하여 영상의 비가시성을 향상시킬 수 있다. 그리고 워터마크 정보를 고려한 임계값을 적응적으로 설정하여 워터마크의 정보량을 안정 적으로 삽입할 수 있도록 보장할 수 있어 비디오 워터마킹을 효과적으로 수행할 수 있다. 제안한 방법은 비디오 비트스트림의 변화가 없기 때문에 기존의 동영상 압축표준과의 호환성을 유지할 수 있으며, 화질적인 측면에서 기존의 방법보다 약 $0.5{\sim}1.0\;dB$가 향상됨을 실험을 통해 확인할 수 있었다.

• BMB Reports
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• 제36권4호
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• pp.409-416
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• 2003

The isoform 1 of cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) from Paenibacillus sp. A11 was purified by a preparative gel electrophoresis. The importance of histidine, tryptophan, tyrosine, and carboxylic amino acids for isoform 1 activity is suggested by the modification of the isoform 1 with various group-specific reagents. Activity loss, when incubated with diethylpyrocarbonate (DEP), a histidine modifying reagent, could be protected by adding 25 mM methyl-$\beta$-cyclodextrin substrate prior to the modification. Inactivation kinetics of isoform 1 with DEP resulted in second-order rate constants ($k_{inactivation}$) of $29.5\;M^{-1}s^{-1}$. The specificity of the DEP-modified reaction for the histidine residue was shown by the correlation between the loss of isoform activity and the increase in the absorbance at 246 nm of N-carbethoxyhistidine. The number of histidines that were modified by DEP in the absence and presence of a protective substrate was estimated from the increase in the absorbance using a specific extinction coefficient of N-carbethoxyhistidine of $3,200\;M^{-1}cm^{-1}$. It was discovered that methyl-$\beta$-CD protected per mole of isoform 1, two histidine residues from the modification by DEP. To localize essential histidines, the native, the DEP-modified, and the protected forms of isoform 1 were digested by trypsin. The resulting peptides were separated by HPLC. The peptides of interest were those with $R_t$ 11.34 and 40.93 min. The molecular masses of the two peptides were 5,732 and 2,540 daltons, respectively. When the data from the peptide analysis were checked with the sequence of CGTase, then His-140 and His-327 were identified as essential histidines in the active site of isoform 1.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2017년도 춘계학술대회 논문집
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• pp.149-149
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• 2017

Aluminum alloys have poor corrosion resistance compared to the pure aluminum due to the additive elements. Thus, anodizing technology artificially generating thick oxide films are widely applied nowadays in order to improve corrosion resistance. Anodizing is one of the surface modification techniques, which is commercially applicable to a large surface at a low price. However, most studies up to now have focused on its commercialization with hardly any research on the assessment and improvement of the physical characteristics of the anodized films. Therefore, this study aims to select the optimum temperature of sulfuric electrolyte to perform excellent corrosion resistance in the harsh marine environment through electrochemical experiment in the seawater upon generating porous films by variating the temperatures of sulfuric electrolyte. To fabricate uniform porous film of 5083 aluminum alloy, we conducted electro-polishing under the 25 V at $5^{\circ}C$ condition for three minutes using mixed solution of ethanol (95 %) and perchloric (70 %) acid with volume ratio of 4:1. Afterward, the first step surface modification was performed using sulfuric acid as an electrolyte where the electrolyte concentration was maintained at 10 vol.% by using a jacketed beaker. For anode, 5083 aluminum alloy with thickness of 5 mm and size of $2cm{\times}2cm$ was used, while platinum electrode was used for cathode. The distance between the two was maintained at 3 cm. Anodic polarization test was performed at scan rate of 2 mV/s up to +3.0 V vs open circuit potential in natural seawater. Surface morphology was compared using 3D analysis microscope to observe the damage behavior. As a result, the case of surface modification showed a significantly lower corrosion current density than that without modification, indicating excellent corrosion resistance.

• 미생물학회지
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• 제25권1호
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• pp.40-45
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• 1987

B Brevibacterium divaricatum FERM 5948 균주로부터 Bdi I RIM 체계에 속하는 BdiI methylase 유천자를 클로닝하여 발현을 조사하였다. Bdi I methylase 유전자의 클로닝을 위해 pBR 322의 EcoRI, BamHl, Sal I 3 군데의 클로닝 site를 이 용했고 1 차 형질전환후 나온 플라스미드를 BdiI으로 자른 뒤 ligation 시키지 않고 형질전환시키는 방법을 이용하였다. 유전 자을 가지는 행질전환체의 선별은 Bdi I methylase에 의해 수정된 채조합 플라스미드는 BdiI 제한효소에 방호된다는 것에 기 초하여 선별하였는데 5.6kb의 EcoRI insert DNA를 가지는 pBDIM 116이 Bdil methylase 유전자플 가지는 것으로 판명 되었다. pBDIM 11&을 가지는 숙주셰포에서 추출한 추출용액에는 S-adenosylmethionine이 있으면 BdiI의 인지부위인 A ATCGAT에만 특정한 methylase 활성이 측정되였다. 11개의 제한효소를 이용하이 제한효소지도를 작성하였고, BdiI r restriction -modification 체계에 관해서 도 논의하였다.

• 생태와환경
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• 제52권1호
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• pp.65-70
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• 2019

본 조사는 월령이 망(보름)인 2016년 4월 22~23일과 5월 25~26일에 낙동강 하굿둑 갑문과 어도 등 총 4개 지점에서 실뱀장어 이동 가능성에 대한 현장 조사를 실시하였다. 실뱀장어는 총 23개체가 채집되었으며, 지점별로 보면 동편 하굿둑에서 22개체, 서편 하굿둑에서 1개체가 채집되었다. 소상지원 시설물 형태 변화, 즉 갑문의 패들 및 어도의 수문 개방 여부, 인조잔디 및 솔의 설치에 따른 실뱀장어의 채집 개체수의 변화를 보면, 동편 하굿둑 갑문의 경우 5개체에서 15개체로 3배 상승하였고, 2번 어도의 경우 0개체에서 2개체로 상승하였다. 본 조사는 2회 조사 결과로 표본수가 작아 모집단에 대한 대표성은 결여되어 있으나, 낙동강 하굿둑 어도와 갑문 모두에서의 실뱀장어를 채집하여 실뱀장어의 하굿둑 어도와 갑문 이용 가능성을 확인하였다. 뿐만 아니라 하굿둑 어도 및 갑문의 소상지원 시설물 형태변화에 따른 실뱀장어 채집 개체수가 현저한 차이를 보여주고 있어, 향후 실뱀장어 회유시기에는 하굿둑 어도 및 갑문을 능동적으로 운영해야 할 것으로 사료된다.