• Reproductive and Developmental Biology
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• v.41 no.1
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• pp.7-16
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• 2017

The knock-in efficiency in the fibroblast is very important to produce transgenic domestic animal using nuclear transfer. In this research, we constructed three kinds of different knock-in vectors to study the efficiency of knock-in depending on structure of knock-in vector with different size of homologous arm on the ${\beta}-casein$ gene locus in the somatic cells; DT-A_cEndo Knock-in vector, DT-A_tEndo Knock-in vector I, and DT-A_tEndo Knock-in vector II. The knock-in vector consists of 4.8 kb or 1.06 kb of 5' arm region and 1.8 kb or 0.64 kb of 3' arm region, and neomycin resistance gene(neor) as a positive selection marker gene. The cEndo Knock-in vector had 4.8 kb and 1.8 kb homologous arm. The tEndo Knock-in vector I had 1.06 kb and 0.64 kb homologous arm and tEndo Knock-in vector II had 1.06 kb and 1.8 kb homologous arm. To express endostatin gene as transgene, the F2A sequence was fused to the 5' terminal of endostatin gene and inserted into exon 7 of the ${\beta}-casein$ gene. The knock-in vector and TALEN were introduced into the bovine fibroblast by electroporation. The knock-in efficiencies of cEndo, tEndo I, and tEndo II vector were 4.6%, 2.2% and 4.8%, respectively. These results indicated that size of 3' arm in the knock-in vector is important for TALEN-mediated homologous recombination in the fibroblast. In conclusion, our knock-in system may help to create transgenic dairy cattle expressing human endostatin protein via the endogenous expression system of the bovine ${\beta}-casein$ gene in the mammary gland.

• Journal of dental hygiene science
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• v.6 no.2
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• pp.113-117
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• 2006

The purpose of this study was to evaluate caries preventive effects of a school-based weekly mouthrinsing program with a 0.2% sodium fluoride solution for five years at elementary school in Yangsan city, which were conducted for 330 children of elementary school from 2000 to 2005. The surveyed data was analyzed with SPSS statistical package. The obtained results were as follows; 1. DMF rate was 58.4% before the mouthrinsing program in 2000 and 48.3% after the program in 2005. DMF rate in 2005 decreased by 10.1% compared to DMF rate in 2000. 2. DT index was 2.46 before the mouthrinsing program and 1.70 after the program. DT index in 2004 decreased by 0.76 compared to DT index in 2000. 3. FT index was 0.55 before the mouthrinsing program and 0.37 after the program. Filled teeth due to decay after the program decreased by 0.18 compared to filled teeth before the program. 4. DMFT score was 2.61 before the mouthrinsing program and 1.64 after the program. DMFT score in 2004 decreased by 0.97 compared to DMFT score in 2000. 5. PHP index was 12.12 before the mouthrinsing program and 5.95 after the program. PHP index in 2005 decreased by 6.17 compared to PHP index in 2000.

• Journal of Power Electronics
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• v.4 no.3
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• pp.161-168
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• 2004

This paper presents a new circuit topology of high-frequency soft switching commutation boost type PWM chopper-fed DC-DC power converter with a loadside auxiliary passive resonant snubber. In the proposed boost type chopper-fed DC-DC power converter circuit operating under a principle of ZCS turn-on and ZVS turn-off commutation, the capacitor and inductor in the auxiliary passive resonant circuit works as the lossless resonant snubber. In addition to this, the voltage and current peak stresses of the power semiconductor devices as well as their di/dt or dv/dt dynamic stress can be effectively reduced by the single passive resonant snubber treated here. Moreover, it is proved that chopper-fed DC-DC power converter circuit topology with an auxiliary passive resonant snubber could solve some problems on the conventional boost type hard switching PWM chopper-fed DC-DC power converter. The simulation results of this converter are illustrated and discussed as compared with the experimental ones. The feasible effectiveness of this soft witching DC-DC power converter with a single passive resonant snubber is verified by the 5kW, 20kHz experimental breadboard set up to be built and tested for new energy utilization such as solar photovoltaic generators and fuel sell generators.

• Journal of the Korea Computer Industry Society
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• v.3 no.9
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• pp.1177-1188
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• 2002

To classify real time Lead pin images in this paper, The image acquisition system was composed to C.C.D, image frame grabber(DT3153), P.C(PentiumIII). I proposed image processing algorithms. This algorithms were composed to real time monitoring, Lead Pin image acquisition, image noise deletion, object area detection, point detection and pattern classification algorithm. The raw images were acquired from Lead pin images using the system. The result images were obtained from raw images by image processing algorithms. In implemental result, The right recognition was 97 of 100 acceptable products, 95 of 100 defective products. The recognition rate was 96% for total 200 Lead Pins.

• Korean Journal of Veterinary Service
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• v.24 no.3
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• pp.243-253
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• 2001

Forty-five Salmonella typhimurium isolates were encountered 8 phage types in which DT197 and U302 were the predominant types. The DT104 type which was first found from pig in Korea, and was resistant to chloramphenicol, streptomycin, sulfamethoxazole/trimethoprim, tetracycline, gentamicin and nalidixic acid. Twenty-two S enteritidis isolates were encountered 5 phage types in which PT4 were the representative (predominant). S enteritidis isolates were susceptible to all antimicrobial agents. As a result of PFGE analysis for S typhimurium and S enteritidis, PFGE patterns was better than phage typing in discriminating of strains. PFGE patterns were not in accord with phage type even though some strain had the same phage types.

• Journal of Chest Surgery
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• v.28 no.6
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• pp.549-556
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• 1995

We investigated the effects of aprotinin, a protease inhibitor, on isolated rat heart subjected to cardioplegia and global ischemia for 4 hours and then reperfused for 40 minutes. Before ischemia, hearts were perfused with either aprotinin 1x105KIU/L[Aprotinin group,n=8 or no aprotinin[control group,n=8 added to Krebs-Henseleite solution for 30 minutes. Hemodynamic and biochemical parameters such as heart rate, LVP, dP/dt, coronary flow and creatine kinase were measured before cardioplegia and after reperfusion 10,20,30,40 minutes. After completion of experiment, wet and dry heart weight were measured for tissue water and water content evaluation. On reperfusion, recovery of LVP of aprotinin group at each time point was significantly better than that of control group[p<0.05 , and of dP/dt at reperfusion 40 minutes[p=0.034 . No statistically significant differences in heart rate, coronary flow and CK were observed between the two groups, but aprotinin group seemed to have better recovery. No significant differences in tissue water and water content were observed between the two group.These results suggest that pretreatment of aprotinin is effective in myocardial preservation in prolonged hypothermic ischemia and reperfusion.

• Biomolecules & Therapeutics
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• v.5 no.4
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• pp.390-401
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• 1997

In order to investigate the pharmacological properties of New Woohwangchungsimwon Liquid (NCL), effects of Woohwangchungsimwon Liquid (CL) and NCL were compared. In isolated rat aorta, NCL and CL showed the relaxation of blood vessels in maximum contractile response to phenylephrine (10$^{-6}$ M) without regard to intact endothelium or denuded rings of the rat aorta. Furthermore, the presences of the inhibitor of NO synthase and guanylate cyclase did not affect the relaxation of NCL and CL. NCL and CL inhibited the vascular contractions induced by acetylcholine, prostaglandin endoperoxide or peroxide in a dose-dependent manner. In conscious spontaneously hypertensive rats (SHRs), NCL and CL significantly decreased heart rate. NCL and CL, at high doses, had a negative inotropic effect that was a decrease of LVDP and (-dp/dt)/(+dp/dt) in the isolated perfused rat hearts, and also decreased the contractile force and heart rate in the isolated rat right atria. In excised guinea-pig papillary muscle, NCL and CL had no effects on parameters of action potential at low doses, whereas inhibited the cardiac contractility at high doses. These results suggested that NCL and CL have weak cardiovascular effects with relaxation of blood vessels and decrease of heart rate, and that this effect is no significant differences between two preparations.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.4 no.5
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• pp.691-708
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• 2010

An important issue of supporting multi-users with diverse quality-of-service (QoS) requirements over wireless networks is how to optimize the systematic scheduling by intelligently utilizing the available network resource while, at the same time, to meet each communication service QoS requirement. In this work, we study the problem of a variety of communication services over multi-media heterogeneous cognitive OFDM system. We first divide the communication services into two parts. Multimedia applications such as broadband voice transmission and real-time video streaming are very delay-sensitive (DS) and need guaranteed throughput. On the other side, services like file transmission and email service are relatively delay tolerant (DT) so varying-rate transmission is acceptable. Then, we formulate the scheduling as a convex optimization problem, and propose low complexity distributed solutions by jointly considering channel assignment, bit allocation, and power allocation. Unlike prior works that do not care computational complexity. Furthermore, we propose the FAASA (Fairness Assured Adaptive Sub-carrier Allocation) algorithm for both DS and DT users, which is a dynamic sub-carrier allocation algorithm in order to maximize throughput while taking into account fairness. We provide extensive simulation results which demonstrate the effectiveness of our proposed schemes.

• Bulletin of the Korean Chemical Society
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• v.33 no.11
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• pp.3544-3550
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• 2012

Cetrizine dihydrochloride (CTZH) is widely used as an anti-allergic drug. Sodium N-bromo-p-toluenesulfonamide or bromamine-T (BAT) is the bromine analogue of chloramine-T (CAT) and is found to be a better oxidizing agent than CAT. In the present research, the kinetics of oxidation of CTZH with BAT in acid and alkaline media was studied at 313 K. The experimental rate laws obtained are: -d[BAT]/dt=$k[BAT][CTZH]^{0.80}[H^+]^{-0.48}$ in acid medium and -d[BAT]/dt=$k[BAT][CTZH]^{0.48}[OH^-]^{0.52}[PTS]^{-0.40}$ in alkaline medium where PTS is p-toluenesulfonamide. Activation parameters and reaction constants were evaluated. The solvent isotope effect was studied using $D_2O$. The dielectric effect is positive. The stoichiometry of the reaction was found to be 1:1 and the oxidation products were identified as 4-chlorobenzophenone and (2-piperazin-1-yl-ethoxy)-acetic acid in both media. The rate of oxidation of CTZH is faster in acid medium. Suitable mechanisms and related rate laws have been worked out.

• Journal of the Korean Chemical Society
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• v.17 no.4
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• pp.298-305
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• 1973

Following results were obtained for the mechanical degradation of polystyrene (for polystyrene itself and when blended with rubber) by roll mastication. 1) The rate of mechanical degradation for polystyrene itself can be represented by the second-order rate equation proposed by Goto. $-\frac{dP_t}{dt} = k_s(P_t-P_{\infty})^2$ Where Pt is the degree of polymerization of the degraded polymer at t minutes and $P{\infty}$ is the final degree of polymerization. 2) The mechanical degradation of polystyrene component in the polystyrene-rubber (SBR, BR) blend system occurred similarly as that of polystyrene itself. 3) Under the experimental conditions the mechanical degradation rate of the polystyrene component of the polystyrene-rubber, (SBR, BR) blend system followed approximately the same second-order equation as that for polystyrene itself.