• 제목/요약/키워드: 4D-Var

검색결과 314건 처리시간 0.027초

차풀 종자로부터 Daucosterol과 Naphthalene glucoside의 분리 (Isolation of Daucosterol and Naphthalene glucoside from Seeds of Cassia mimosoides var. nomame Makino)

  • 박준홍;권순진
    • 한국자원식물학회지
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    • 제22권1호
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    • pp.26-30
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    • 2009
  • 본 연구는 속리산국립공원 내 쌍곡계곡에 분포하는 식물상을 조사하기 위해 수행되었고 조사기간은 2006년 3월부터 2007년 6월까지 조사하였다. 식물상을 조사한 결과, 88과 242속 342종 52변종 7품종 등 총 401분류군이 확인되었다. 한국특산식물은 개비자나무, 키버들, 개족도리, 할미밀망, 민산초나무, 청괴불나무, 병꽃나무, 분취 등 총 8분류군이 조사되었다. 환경부 지정 멸종위기식물은 망개나무 등 1분류군이며, 산림청 지정 희귀식물은 개족도리, 고란초, 망개나무, 백작약, 태백제비꽃, 꼬리진달래, 말나리 등 7분류군이 조사되었다. 이들의 보전을 위해서는 향후 희귀식물에 대한 구체적인 보전방안과 번식방법 등이 논의되어야 할 것으로 판단된다. 자원식물은 관상용 식물 220분류군, 식용 식물 239분류군, 약용 식물 281분류군, 기타용 식물 206분류군으로 나타났다. 귀화식물은 오리새, 소리쟁이, 취명아주, 아까시나무, 붉은토끼풀, 토끼풀, 큰달맞이꽃, 비짜루국화, 붉은서나물, 개망초, 망초, 뚱딴지, 서양민들레 등 13분류군이 관찰되었으며, 귀화율은 약 3.24%, 자연파괴도는 약 4.53%로 나타났다.

한국에서의 오이모자익 바이러스의 기주범위에 관한 연구 (Studios on the Host Range of Cucumber Mosaic Virus in Korea)

  • 정봉조;박해철;이순형
    • 한국응용곤충학회지
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    • 제14권4호
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    • pp.185-192
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    • 1975
  • 가. CMV의 기주조사결과, 43과 145종을 공시하여 27과 71종이 기주로 판명되었으며 그중 24두 57종이 Systemic mosaic을 나타내었다. 나. CM V의 새로운 기주로 확인된 식물은 쇠별꽃 쇠무릎, 아게라탐, 중대가리풀, 천인국, 지칭개, 씀바귀, 그늘취, 유채, 다닥냉이, 수염가래꽃, 좀명아주, 실사초, 깨풀, 새콩, 벌노랑이, 팥, 큰기린초, 들깨풀, 들깨, 개곽량, 아마, 주름잎, 버어베나의 24종이 판명되었다. 다. 다른 연구자의 보고에는 감수성으로 나타났으나 본 시험에서 음성으로 나타난 식물은 양파, 맨드라미, 당근, 쑥, 과꽃, 망초, 해바라기, 만수국, 봉선화, 양배추, 배추, 스위트알리슘, 무, 고구마, 대두, 강남콩, 나팔백합, 양귀비, 수수, 밀, 옥수수, 소루쟁이, 채송화 등 23종이었다.

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배지(培地) 및 생장(生長)호르몬에 따른 기내배양(器內培養)된 포푸라속(屬) 8개(個) 수종(樹種)의 기관형성반응(器官形成反應) (Morphogenetic Responses according to Media and Growth Regulators in vitro Culture of Eight Populus spp.)

  • 김지문;권기원;문흥규;이재순
    • 농업과학연구
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    • 제14권2호
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    • pp.205-212
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    • 1987
  • 기내배양(器內培養)된 포푸라속(屬) 8개(個) 수종(樹種)의 줄기조직편(組織片)(stem segment)과 양버들의 엽(葉), 엽병(葉柄), 절간조직(節間組織)을 재료(材料)로 배지(培地), 생장(生長)호르몬, 치상부위(置床部位)에 따른 기관형성능력(器官形成能力)을 시험(試驗)하여 다음 결과(結果)를 얻었다. 1. 줄기조직편(組織片)에서 shoot 유도(誘導)는 절(節)(section) 및 수종(樹種)에 따라 많은 차이(差異)를 보였다. 배양(培養) 4주후(週後)에 Leuce 및 Tacamahaca절(節)에 속(屬)하는 수종(樹種)은 부정아유도(不定芽誘導)가 안되었으며 액아(腋芽)나 잠아(潛芽)로부터 shoot가 신장(伸長)되었고, Aigeiros절(節)의 양버들은 조직편당(組織片當) 5.4개(個)의 가장 좋은 부정아발생(不定芽發生)을 보였다. 2. 양버들은 배지(培地) 및 생장(生長)호르몬에 따라 기관형성(器官形成)에 큰 차이(差異)를 보여 엽(葉)의 abaxial side 배양(培養)이 adaxial side배양(培養)보다 부정아발생(不定芽發生)이 양호(良好)하였으며 WPM배지(培地)에 $0.2mg/{\ell}$ BAP가 첨가(添加)된 경우 배양(培養) 5주후(週後)에 조직편당(組織片當) 평균(平均) 103개(個)의 부정아(不定芽)가 유도(誘導)되었다. 3. 2,4-D는 양버들의 부정아유도(不定芽誘導)에는 부적당(不適當)하였으며 callus의 형성(形成)을 촉진(促進)하였고, NAA 혹은 NAA와 BAP의 결합첨가(結合添加)는 모든 조직편(組織片)에서 뿌리의 발생(發生)을 촉진(促進)하였다.

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헛개나무(Hovenia dulcis var. koreana) 과병 열수추출분획물의 간보호 및 혈중 알콜 저하 작용 (Hepatoprotective and Blood Alcohol Lowering Effects of Fruit Peduncle Extract of Hovenia dulcis var. Koreana in the In Vitro and in Vivo Animal Models)

  • 나천수;정남철;양규환;김세현;정하숙;동미숙
    • 약학회지
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    • 제48권1호
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    • pp.34-40
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    • 2004
  • Hovenia dulcis which is distributed in Korea, China and Japan is known to show hepatoprotctive effect and reduce the acute alcohol toxicity. In this study, the hepatoprotective effect against the chemically induced experimental liver injury models and lowering effect of blood alcohol level in animal models acutely administered alcohol by the peduncle extracts of Hovenia dulcis var. koreana were investigated. HdfHW-1, and HdfM-1 which are the extracts of fruit peduncles and young branches with hot water or 70% methanol and followed with 100% methanol, were significantly reduced the $CCl_4$ or D-galactosamine/LPS induced damage in sliced liver. The hot water or methanol extracts of fruit peduncle protected dose-dependently against $CCl_4$ induced toxicity in primary hepatocyte culture and particularly, the amount of LDH release was reduced to the control level at 500 $\mu\textrm{g}$/ml of hot water extracts. HdfHW-1 also decreased the hepatotoxicity induced by $CCl_4$ in rats. The active components of HdfHW-1 seemed to be high molecular weights because 0.2 M NaCl HdfHW-1 fraction was the most effective among NaCl fractions of HdfHW-1 eluted with various concentrations of NaCl on DEAE 650C column chromatography. HdfM and HdfHW were significantly reduced the levels of blood alcohol in rats and mice administered 40% alcohol. These results indicated that the hot water or methanol extracts of fruit peduncle of Hovenia dulcis var. koreana have hepatoprotective effect and may be reduce alcohol toxicity.

Enhancement of enzymatic activity of ${\beta}-cyclodextrin$ glucanotransferase from Bacillus firmus var. alkalophilus by site-directed mutagenesis

  • 이광우;신현동;이용현
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2001년도 추계학술발표대회
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    • pp.656-659
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    • 2001
  • Cyclodextrin glucanotransferase (CGTase) (EC 2.4.1.19) use starch to produce cyclic maltooligosaccharides (cyclodextrins, CDs) which are of interest in various applications. To obtain a novel CGTase having high CD-forming activity, ${\beta}-cyclodextrin$ glucanotransferase $({\beta}-CGTase)$ from Bacillus firmus var. alkalophilus was modified through site-directed mutagenesis and constructed five mutants, H59T, H59Q, Y96M, 9O-PPI-93, and ${\Delta}(148-154)D$, respectively. Y96M and ${\Delta}(148-154)D$ showed much higher level of conversion yields of starch into CDs from 28.6% to about 39% compared to wild-type ${\beta}-CGTase$, respectively, but 90-PPI-93 maintained similar convesion yields of starch to CDs. And their ${\beta}-CD$ ratios to total CDs were not changed and maintained, and convesion yields to linear maltooligosaccharides of all mutants were not changed significantly. These results indicates that five mutations of ${\beta}-CGTase$ from Bacillus firmus var. alkalophilus appears to be important roles for increase of overall CD production rather than change of its product specificity, especially.

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아왜나무와 종가시나무의 생육에 대한 지중매입형 컨테이너의 효과 (Effects of Underground Container Types on Plant Growth of Viburnum odoratissimum var. awabuki and Quercus glauca)

  • 최수민;신현철;허근영;정현종
    • 한국환경복원기술학회지
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    • 제15권3호
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    • pp.67-74
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    • 2012
  • This study was carried out to cultivate evergreen-leaved tree container seedlings having low cost of equipment and easy to control. Two species of V. odoratissimum var. awabuki and Q. glauca planting in respective underground container were showed survival rate above 90%. The Relative growth rates of height was good in non-treatment and root collar diameter was no significant difference between treatments. The T/R ratio was respectively 1.1, 1.0 in container type of F, G. and root's biomass products was more effective than others. A container type of F showed better Quailty index than others with 16.2 values. Q. glauca was higher T/R ratio in root development than shoot and the quality index was high in container type of E, D with 4.0. 3.5, respectively. On the basis of the results obtained in this study, we can concluded that the underground container type was suitable to F in V. odoratissimum var. awabuki and to D or E in Q. glauca.

자외선이 조사된 사람피부 섬유아세포에서 흰털오가피 잎추출물의 항산화작용 (Antioxidative Activity of Extracts of Acanthopanax divaricatus var. albeofructus Leaves in Human Dermal Fibroblast Irradiated by UVA)

  • 신애향;유수연;노빛나;김자인;김옥경;박원봉
    • 약학회지
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    • 제51권4호
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    • pp.229-234
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    • 2007
  • We investigated antioxidative activity of the water and ethanol extracts of leaves of Acanthopanax divaricatus var. albeofructus in human dermal fibroblast (HDFs) irradiated by UVA. The irradiation of UVA did not affect the cell viability of HDFs. The antioxidative activity of the extract was investigated by xylenol orange, TBARS (thiobarbituric acid reactive substances) and antioxidant enzyme assay. Both extracts showed H202 scavenging activity and inhibited lipid peroxidation in HDF cells irradiated by UVA. The extracts also recovered enzyme activity in the same cells.

좁은잎산사나무 열매의 화학성분 (Chemical Components from the Fruits of Crataegus pinnatifida var. psilosa)

  • 박시우;육창수;이형규
    • 생약학회지
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    • 제25권4호통권99호
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    • pp.328-335
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    • 1994
  • The ether soluble fraction of the methanol extract from the fruits of Crataegus pinnatifida var. psilosa was applied to silica gel column chromatography, eluted step-gradiently with a mixture of dichloromethane and methanol, repeatedly, and five compounds were isolated. These compounds are identified as ursolic acid(I), corosolic acid(II) and euscapic acid (III), ${\beta}-sitosteryl-3-O-{\beta}-_D-glucopyranoside(IV)$ and hyperin(V).

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산조인 (酸棗仁)의 Flavonoid성분 (Flavonoids from the Seeds of Zizyphus jujuba var. spinosa)

  • 이소영;이주영;김주선;이제현;강삼식
    • 생약학회지
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    • 제43권2호
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    • pp.127-136
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    • 2012
  • Eight flavonoids were isolated from the BuOH fraction of the 70% EtOH extract of Zizyphus jujuba var. spinosa seeds along with three known compounds, daucosterol (1), butyl ${\beta}$-D-fructofuranoside (2), and magnoflorine (4). On the basis of spectroscopic methods and comparison with literature values their structures were elucidated as 6"'-feruloylspinosin (3), nicotiflorin (5), 6"'-p-coumaroylspinosin (6), isospinosin (7), 6"'-vanilloylspinosin (8), spinosin (9), hovetrichoside C (10), and camelliaside B (11). Compounds 1, 2, 10, and 11 were isolated from this plant for the first time.

가치-부가적 성과 관점에 따른 효과적인 목표설정과 실사례 (The Effective Goal-Setting and The Practice based on Value-Added Results(VAR))

  • 신택현
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2004년도 추계학술대회 논문집
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    • pp.1731-1736
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    • 2004
  • The purpose of this article is to introduce a useful methodology of effective goal-setting for the team-level units. As a way to overcome some common symptoms in terms of Strategic Performance Evaluation System such as lack of knowledge on goal-setting, disconnection of process, problem of judging the degree of difficulty about objectives, limits of staff departments evaluation, fairness and authority of evaluators, weakness in coaching technique, and quantity or figure-oriented evaluation, to name a few, and to seek a more plausible goal-setting methodology, the author suggests a persuasive goal-setting concept: VAR(Value-Added Results). VAR, as the end-results, is the team contributions that add value to the organization, and it results from the team's activities. In addition to these goal-setting technique based on the concept of value-added results, several aspects should be improved for Strategic Performance Evaluation System to be implemented more effectively. They are: 1) shift from MBO to MP & D(Managing Performance & Development), 2) impartial exercise of evaluation authority as a organizational public assets, 3) reinforcement of maternal leadership and servantship instead of paternal leadership, 4) utilization of IT-based evaluation system.

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