• Title/Summary/Keyword: 4D-Media

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Optimization of Mycelial Growth of Entomogenous fungi of the Genus Cordyceps (동충하초속균의 균사생장최적화)

  • Hong, In-Pyo;Nam, Sung-Hee;Jung, I-Yeon;Sung, Gyoo-Byung;Nam, Hack-Woo;Kang, Seok-Woo;Hur, Hyeon;Lee, Min-Woong;Guo, Shun-Xing
    • Journal of Mushroom
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    • v.2 no.3
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    • pp.149-156
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    • 2004
  • This study was carried out to obtain basic data on physiological characteristics for an artificial cultivation of fruiting body of Cordyceps. Specimens such as Cordyceps longissima, C. militaris and C. pruinosa were collected at Mt. Halla of Cheju island in July, 2003. Among four different culture media which have been used for culture of mushrooms, MCM medium was selected for the favorable culture medium of the Cordyceps tested. The initial pH of solid medium for mycelial growth of Cordyceps was good in the range of pH 5.0~7.0 lower than 8.0. The mycelial growth of C. longissima was most favorable on culture media supplemented with glucose, one of monosaccharides. In C. militaris, nine carbon sources were favorable to the mycelial growth as compared with control among 11 carbon sources. Six nitrogen sources were favorable to the mycelial growth of C. longissima as compared with control among 9 carbon sources; namely, the mycelial growth of C. longissima was most favorable on culture media contained potassium nitrate, and followed in order by ammonium citrate and sodium nitrate in 4 weeks incubation.

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연초(Nicotiana tabacum cv. Xanthi) 배양세포로부터 Ubiquinone-10 생산을 위한 현탁배양

  • 양덕춘;최광태;박지창;강신웅;이정명
    • Journal of the Korean Society of Tobacco Science
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    • v.21 no.1
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    • pp.64-69
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    • 1999
  • The effect of phytohormones, light and phosphate on in vitro production of ubiquinone 10 from the suspension cultures of Nicotiana tabacum cv. Xanthi callus was investigated. The inoculum size and cultured time in the suspension culture had to be at least over 2 % of medium volume at 15 days for the excellent growth of Xanthi callus. The growth of Xanthi callus in the suspension culture was improved by addition of NAA and 2,4-D, especially NAA 1.0mg/1 alone, at the light condition. The optimal concentration of phytohormone was 0.1 mg/l 2.4-D and 1.0 mg/l NAA for productivity of ubiquinone 10 in the suspenseion of Xanthi callus. Addition of 3mM KH$_2$PO$_4$ to the medium was more effective in promoting ubiquinone-l0 formation than other concentration in the light condition. Content and production of ubiquinone-l0 in the suspension cultures of Xanthi callus were the highest at the MS media containing 0.5mg/L kinetin, 0.5mg/L 2,4-D, 1.0mg/1 NAA, 3mM phosphate and 2 % inoculum in the light.

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Somatic Embryogenesis and Plant Regeneration from Immature Zygotic Embryo Culture of Wasabia japonica Matsum. (고추냉이의 미숙배배양으로부터 체세포배 발생과 식물체 재분화)

  • 은종선;고정애;김영선;김명준
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.4
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    • pp.207-211
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    • 1995
  • Immature zygotic embryos from immature seeds of Wasabia japonica (cv Dalma) were isolated and cultured on modified MS medium supplemented with 2,4-D, IAA, and BA. Immature zygotic embryos were classified into torpedo shape and cotyledon stage. The highest rates of callus formation were obtained of 1.0mg/L IAA(torpedo stage, 90.0%)and 1.0mg/L 2,4D plus 0.1mg/L BA(cotyledany stage,84.3%). Somatic embryos after 60 days of culture. These numerous somatic embryo could be seperated and subcultured on the same media for further propagation. After 90 days of culture, most somatic embryos were developed well organized embryos which were able to produce into whole plants.

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Effects of Proline and Gelatin on hCTLA4Ig Production in Transgenic Rice Suspension Cells (형질전환 벼 현탁세포를 이용한 hCTLA4Ig 생산에서 proline과 gelatin이 미치는 영향)

  • Song, Mi-Na;Cheon, Su-Hwan;Kwon, Jun-Young;Choi, Sung-Hun;Kim, Dong-Il
    • KSBB Journal
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    • v.24 no.3
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    • pp.246-252
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    • 2009
  • Rice cells were transformed to express human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) using RAmy3D promoter. hCTLA4Ig was produced and secreted into culture media inducibly when sugar was depleted. The obstacles of this system are the cell death and release of proteases by sugar starvation. These problems resulted in the losses of stability and productivity of hCTLA4Ig. Therefore, the effects of proline as an inhibitor of cell death were investigated. When 4 mM proline was added in sugar-free media, the cell death and release of proteases were reduced. As a consequence, the production of hCTLA4Ig was enhanced. In addition, the effects of protein stabilizers such as gelling agents were studied. It was found that the application of 0.01 g/L gelatin led to an increase in hCTLA4Ig production. This increase might be originated from the stabilization of hCTLA4Ig. In conclusion, the production of hCTLA4Ig could be enhanced by the additions of proline and gelatin in transgenic rice cell cultures.

Korean Traditional Music Genre Classification Using Sample and MIDI Phrases

  • Lee, JongSeol;Lee, MyeongChun;Jang, Dalwon;Yoon, Kyoungro
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.12 no.4
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    • pp.1869-1886
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    • 2018
  • This paper proposes a MIDI- and audio-based music genre classification method for Korean traditional music. There are many traditional instruments in Korea, and most of the traditional songs played using the instruments have similar patterns and rhythms. Although music information processing such as music genre classification and audio melody extraction have been studied, most studies have focused on pop, jazz, rock, and other universal genres. There are few studies on Korean traditional music because of the lack of datasets. This paper analyzes raw audio and MIDI phrases in Korean traditional music, performed using Korean traditional musical instruments. The classified samples and MIDI, based on our classification system, will be used to construct a database or to implement our Kontakt-based instrument library. Thus, we can construct a management system for a Korean traditional music library using this classification system. Appropriate feature sets for raw audio and MIDI phrases are proposed and the classification results-based on machine learning algorithms such as support vector machine, multi-layer perception, decision tree, and random forest-are outlined in this paper.

The study on the $CO_2$ fixation and algae reproduction by microalgae Chlorella ellipsoidea (Chlorella ellipsoidea를 이용한 $CO_2$ 고정 및 미세조류 증식효과 검토)

  • 강창민;홍순강
    • Journal of environmental and Sanitary engineering
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    • v.15 no.1
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    • pp.39-45
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    • 2000
  • The purpose of this study was conducted to indentified the fixing quality of $CO_2$, the most important greenhouse effect gas, by microalgae Chlorella ellipsoidea in batch test apparatus. The glass flask of $1.4{\ell}$ culture media which was saturated with 99.99% pure $CO_2$ gas was setted water bath of $25^{\circ}C$, 5000Lux, and seeded 100$m\ell$ algae liquid. We checked the change of inorganic carbon concentration and algae population with time in culture media. The result were next: the growth of algae population relied on aquatic IC(inorganic carbon) concentration. And the pH was increased with decrease of IC concentration. The growth of algae population had positive correalation with $CO_2$ concentration, and the coefficient of correlation was 0.982. The specific growth rate($\mu$) of Chlorella ellipsoidea was 1.104/d, the maximum specific growth $rate({\mu}_{max}$) of 9.21/d, and helf velocity constant($K_s$) of $259mg/{\ell}$ by Monod equation.

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Study on Engineering Barrier Role in Nuclear Waste Disposal

  • Hua, Zhang;Jianwen, Yang;Baojun, Li;Shanggeng, Luo
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2004.02a
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    • pp.73-82
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    • 2004
  • This paper studies the leaching behaviors of pyrochlore-rich synroc incorporated 46.8wt% simulated actinides waste under the five simulated geological disposal media, which included the bentonite, granite, granite + ferroferric oxide, granite + cement, bentonite + ferroferric oxide, respectively. The mass loss rates reached to equilibrium after 182 day and was 10-7 g/$\textrm{mm}^2{\cdot}d$. That suggests the mass loss rate of pyrochlore-rich synroc, loaded 46.8wt% actinides waste, was very low. The surfaces of the leached specimens were analyzed by XRD, SEM/EDS. The experimental results show that the pyrochlore-rich synroc samples in the systems, which contained bentonite and cement, have two new phases formed on the leached specimens surface at $90^{\circ}C$ for 728d; The bentonite and cement can retard the elements leaching; $Fe_3O_4$ can speed the elements leaching; Expect for Ti ion depleted on the sample surface, other ion, such as U, Zr, AI, Ca, were in equable states and Ba ion was enriched during test time, which indicated the simulated disposal media have good ability to retard the leaching behavior of the pyrochlore-rich synroc.

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Heuristic Method for Computing Progressive Mesh Representation between Two Polygonal Models (두 다면체 모델 사이의 점진적 표현을 계산하는 휴리스틱 방법)

  • Yoon, Won-Young;Choi, Jung-Ju;Lee, In-Kwon
    • Journal of the Korea Computer Graphics Society
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    • v.9 no.4
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    • pp.25-30
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    • 2003
  • 본 논문에서는 서로 다른 개수의 정점을 가지는 두 다면체 사이의 점진적 다면체 모델 표현(Progressive Mesh Representation)을 계산하는 휴리스틱 방법을 제시한다. 정점의 개수가 각각 n, k개 인 두 다면체 모델 $M^n$, $M^k$ (n > k)에 대하여 $M^n$에서 서로 다른 k개의 정점을 선택한다. 선택된 k개의 정점을 기준으로 $M^n$의 모든 정점에 대한 클러스터링을 수행하여 k개의 정점군(Vertex Set)을 생성한다. $M^n$을 간략화하여 k개의 정점만을 가지는 모델 $M^{k'}$의 위상정보(Topology)를 $M^k$와 동일하게 유지하기 위하여 $M^n$ 정점군들의 위상정보를 수정한다. 수정 생성된 정점군 내에서 선분병합(Edge Collapse)을 수행하면, 위상정보를 유지하면서 $M^n$에서 $M^k$로 변화하는 점진적 다면체 모델 표현을 얻을 수 있다. $M^{k'}$$M^k$의 정점간의 기하학적 위치차이를 선형보간하여 선분병합이 일어날때 마다 반영하면 $M^n$에서 $M^k$로 기하정보를 부드럽게 유지하면서 변화하는 점진적 다면체 모델 표현을 얻을 수 있다. 본 논문의 연구결과는 기존의 DLoD(Discrete Level of Detail)를 지원하는 게임을 CLoD(Continuous Level of Detail)를 지원하는 게임으로 확장하는 등의 다양한 컴퓨터 그래픽스 응용문제에 사용할 수 있다.

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Android Platform based Gesture Recognition using Smart Phone Sensor Data (안드로이드 플랫폼기반 스마트폰 센서 정보를 활용한 모션 제스처 인식)

  • Lee, Yong Cheol;Lee, Chil Woo
    • Smart Media Journal
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    • v.1 no.4
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    • pp.18-26
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    • 2012
  • The increase of the number of smartphone applications has enforced the importance of new user interface emergence and has raised the interest of research in the convergence of multiple sensors. In this paper, we propose a method for the convergence of acceleration, magnetic and gyro sensors to recognize the gesture from motion of user smartphone. The proposed method first obtain the 3D orientation of smartphone and recognize the gesture of hand motion by using HMM(Hidden Markov Model). The proposed method for the representation for 3D orientation of smartphone in spherical coordinate was used for quantization of smartphone orientation to be more sensitive in rotation axis. The experimental result shows that the success rate of our method is 93%.

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Cache Optimization on Hot-Point Proxy Caching Using Weighted-Rank Cache Replacement Policy

  • Ponnusamy, S.P.;Karthikeyan, E.
    • ETRI Journal
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    • v.35 no.4
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    • pp.687-696
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    • 2013
  • The development of proxy caching is essential in the area of video-on-demand (VoD) to meet users' expectations. VoD requires high bandwidth and creates high traffic due to the nature of media. Many researchers have developed proxy caching models to reduce bandwidth consumption and traffic. Proxy caching keeps part of a media object to meet the viewing expectations of users without delay and provides interactive playback. If the caching is done continuously, the entire cache space will be exhausted at one stage. Hence, the proxy server must apply cache replacement policies to replace existing objects and allocate the cache space for the incoming objects. Researchers have developed many cache replacement policies by considering several parameters, such as recency, access frequency, cost of retrieval, and size of the object. In this paper, the Weighted-Rank Cache replacement Policy (WRCP) is proposed. This policy uses such parameters as access frequency, aging, and mean access gap ratio and such functions as size and cost of retrieval. The WRCP applies our previously developed proxy caching model, Hot-Point Proxy, at four levels of replacement, depending on the cache requirement. Simulation results show that the WRCP outperforms our earlier model, the Dual Cache Replacement Policy.