• Title/Summary/Keyword: 4CBP

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Improved Degradation of 4-Chlorobiphencyl, 2,3-Dihydroxybiphenyl, and Catecholic Compounds by Recombinant Bacterial Strains

  • Kim, Ji-Young;Kim, Youngsoo;Lee, Kyoung;Kim, Chi-Kyung
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.1
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    • pp.56-60
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    • 2001
  • The pcbC gene encoding (4-chloro-)2,3-dihydroxybiphenyl dioxygenase was cloned from the genomic DNA of Pseudomonas sp. P20 using pKT230 to construct pKK1. A recombinant strain, E. coli KK1, was selected by transforming the pKK1 into E. coli XL1-Blue. Another recombinant strain, Pseudomonas sp. DJP-120, was obtained by transferring the pKK1 of E. coli KK1 into Pseudomonas sp. DJ-12 by conjugation. Both recombinant strains showed a 23.7 to 26.5 fold increase in the degradation activity to 2,3-dihydroxybiphenyl compared with that of the natural isolate, Pseudomonas sp. DJ-12. The DJP-120 strain showed 24.5, 3.5, and 4.8 fold higher degradation activities to 4-chlorobiphenyl, catechol, and 3-methylcatechol than DJ-12 strain, respectively. The pKK1 plasmid of both strains and their ability to degrade 2,3-dihydroxybiphenyl were stable even after about 1,200 generations.

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Inhibitory Mechanism on NF-${\kappa}B$ Transactivation by Dexamethasone in Pulmonary Epithelial Cells (폐상피세포에서 Dexamethasone에 의한 NF-${\kappa}B$ Transactivation 억제기전에 관한 연구)

  • Lee, Kye-Young;Kim, Yoon-Seop;Ko, Mi-Hye;Park, Jae-Seok;Jee, Young-Koo;Kim, Keun-Youl;Kwak, Sahng-June
    • Tuberculosis and Respiratory Diseases
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    • v.48 no.5
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    • pp.682-698
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    • 2000
  • Glucocorticoid receptor (GR) functions as a suppressor of inflammation by inhibiting the expression of many cytokine genes activated by NF-${\kappa}B$. The goal of this study is to investigate the mechanism by which GR repress NF-${\kappa}B$ activation in lung epithelial cells. We used A549 and BEAS-2B lung epithelia! cell lines. Using Ig$G{\kappa}$-NF-${\kappa}B$ luciferase reporter gene construct, we found that dexamethasone significantly suppressed TNF-$\alpha$-induced NF-${\kappa}B$ activation and the overexpression of GR showed dose-dependent reduction of TNF-$\alpha$-induced NF-${\kappa}B$ activity in both cell lines. However, DNA binding of NF-${\kappa}B$ induced by TNF-$\alpha$ in electromobility shift assay was not inhibited by dexamethasone. Super shift assay with anti-p65 antibody demonstrated the existence of p65 in NF-${\kappa}B$ complex induced by $\alpha$ Western blot showed that $I{\kappa}B{\alpha}$ degradation induced by TNF-$\alpha$ was not affected by dexamethasone and $I{\kappa}B{\kappa}$ was not induced by dexamethasone, neither. To evaluate p65 specific transactivation, we adopted co-transfection study of Gal4-p65TA1 or TA2 fusion protein expression system together with 5xGal4-luciferase vector. Co-transfection of GR with Gal4-p65TA1 or TA2 repressed luciferase activity profoundly to the level of 10-20% of p65TA1- or TA2-induced transcriptional activity. And this transrepressional effect was abolished by co-transfection of CBP of SRC-1 expression vectors. These results suggest that GR-mediated transrepression of NF-${\kappa}B$ in lung epithelial cells is through competing for binding to limiting amounts of transcriptional coactivators, CBP or SRC-1.

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Study on Electroluminescence of the Phosphorescent Iridium(III) Complex Prepared by Ultrasonic Wave (초음파 합성법을 이용한 이리듐계 인광 물질 합성과 합성된 인광 물질의 전계 발광 특성 분석)

  • Yu, Hong-Jeong;Chung, Won-Keun;Chun, Byung-Hee;Kim, Sung-Hyun
    • Korean Chemical Engineering Research
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    • v.49 no.3
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    • pp.325-329
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    • 2011
  • $Ir(pmb)_{3}$(Iridium(III)Tri(1-phenyl-3-methylbenzimidazolin-2-ylidene-$C,C^{2'}$ ) was synthesized to develop a deep blue-emitting Ir(III) complex. We suggested the ultrasonic reactor to enhance the poor reaction yield of $Ir(pmb)_{3}$. The ultrasonic wave enhanced the reaction yield of $Ir(pmb)_{3}$ because the ultrasound helped non-soluble reactants disperse efficiently and produced free radial during the reaction. The maximum yield of $Ir(pmb)_{3}$ was 42.5%, which was 4 times higher than conventional method. Organic light emitting devices were fabricated with the synthesized mer-$Ir(pmb)_{3}$ which emitted at 405 nm. A range of host materials with large bandgaps (UGH2, mCP and CBP) were tested for developing a deep blue emitting device. In case of the device with mCP as the host material, it emitted deep blue and performed quite well relative to the other host materials tested.

A Study on the Recovery of Zn from Electric Arc Furnace Dust by Carbon Reduction

  • Joo, Sung-Min;Kim, Hyung-Seok;Ahn, Ji-Whan;Kim, Hwan;Lee, Kyung-Hoon;Sung, Ghee-Woong;Kim, Jang-Su;Lee, Park-Seok
    • Proceedings of the IEEK Conference
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    • 2001.10a
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    • pp.398-403
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    • 2001
  • There is a potential usability of electric arc furnace(EAF) dust produced during the iron manufacturing process as a recycled resource, because valuable materials such as Zn, Pb and Fe are contained in it. In this study, metallic Zn was recycled from the fine electric arc furnace dust by a solid state reduction method using carbon at relatively low temperature. It was possible to recover metallic zinc by using of high vapour pressure of zinc with this reduction method. The feasibility of recycled zinc for cold bonded pellet(CBP) was investigated. The main composition of EAF dust were franklinite(ZnFe$_2$O$_4$), magnetite(Fe$_3$O$_4$) and zincite(ZnO), and Pb and Cl were completely removed by a heat treatment in oxidation environment. The reduction ratio increased as the solid carbon content increased, and it increased with decreasing of dust particle size and increasing of compaction pressure due to a increase of contact area.

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Pollen analysis of the Lake Hane, Ohda-city, Shimane Prefecture, Southwest Japan (남서 일본 도근현 大田市 파근호 퇴적물의 화분분석)

  • ;Masami Watanabe;Tadashi Nakamura
    • The Korean Journal of Quaternary Research
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    • v.17 no.1
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    • pp.39-47
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    • 2003
  • The pollen of the sediments from the Lake Hane were analysed for reconstruction of vegetation history around Ohda-City in Southwest Japan, and the correlation with the pollen zone reported for the Lake Shinji. From the pollen assemblages of 29 samples from core OH94 (19.7 m), two pollen zone were established, and they were subdivided into five subzones in ascending order as following; Cyclobalanopsis-Catanopsis pollen zone (Cyclobalanopsis subzone, Catanopsis subzone and, Podocarpus subzone), and Gramineae pollen zone (Cryptomeria subzone and Cyclobalanopsis-Quercus subzone). The K-Ah volcanic ash is identified in the Cyclobalanopsis-Catanopsis pollen zone. We estimated the beginning of the Cyclobalanopsis-Catanopsis pollen zone was before c.a. 6,300 BP.

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Free fatty acid-induced histone acetyltransferase activity accelerates lipid accumulation in HepG2 cells

  • Chung, Sangwon;Hwang, Jin-Taek;Park, Jae Ho;Choi, Hyo-Kyoung
    • Nutrition Research and Practice
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    • v.13 no.3
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    • pp.196-204
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    • 2019
  • BACKGROUND/OBJECTIVES: Non-alcoholic fatty liver disease (NAFLD) is a common metabolic disease triggered by epigenetic alterations, including lysine acetylation at histone or non-histone proteins, affecting the stability or transcription of lipogenic genes. Although various natural dietary compounds have anti-lipogenic effects, their effects on the acetylation status and lipid metabolism in the liver have not been thoroughly investigated. MATERIALS/METHODS: Following oleic-palmitic acid (OPA)-induced lipid accumulation in HepG2 cells, the acetylation status of histone and non-histone proteins, HAT activity, and mRNA expression of representative lipogenic genes, including $PPAR{\gamma}$, SREBP-1c, ACLY, and FASN, were evaluated. Furthermore, correlations between lipid accumulation and HAT activity for 22 representative natural food extracts (NExs) were evaluated. RESULTS: Non-histone protein acetylation increased following OPA treatment and the acetylation of histones H3K9, H4K8, and H4K16 was accelerated, accompanied by an increase in HAT activity. OPA-induced increases in the mRNA expression of lipogenic genes were down-regulated by C-646, a p300/CBP-specific inhibitor. Finally, we detected a positive correlation between HAT activity and lipid accumulation (Pearson's correlation coefficient = 0.604) using 22 NExs. CONCLUSIONS: Our results suggest that NExs have novel applications as nutraceutical agents with HAT inhibitor activity for the prevention and treatment of NAFLD.

Effects of Hwadamtongrak-Tang on the changes of Cerebral Flow in the rats (화담통락탕(化痰通絡湯)이 백서의 뇌혈류변화 및 허혈성 뇌손상에 미치는 영향)

  • Park, Hyoung-Bae;Yang, Seung-Jung;Wei, Tung-Sheun;Park, Hye-Sun;Jeon, Sang-Yoon;Hong, Seok
    • Herbal Formula Science
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    • v.14 no.1
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    • pp.105-119
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    • 2006
  • Objectives & Methods : This present study was performed to investigate the effect of Hwadamtongrak-Tang extract (HTT) on the regulation of cerebral hemodynamics in terms of regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP)] in normal and cerebral ischemic rats. Also the effects of HTT on changes in local blood flow, inhibition of LD H activity in neuronal cells, and levels of cytokine production in the serum were determined in the ischemic rat model. The major findings are summarized below. Results : 1. HTT significantly increased rCBF in a dose-dependent manner, but MABP was not changed by HTT treatment. These results suggest that HTT may increase rCBF by dilating cerebral arterial diameter. 2. HTT-induced increase in rCBF was blocked by pretreatment with cyclooxygenase inhibitor indomethacin (IDN, 1 mg/kg, i.p.) and MABP was significantly increased by ID N. 3. Pretreatment of methylene blue $(MTB,\;10\;{\mu}g/kg,\;i.p.)$, an inhibitor of guanylate cyclase, significantly decreased both rCBP and MABP in HTT-treated rats. 4. HTT treatment significantly increased rCBP to a stable level during the period of cerebral reperfusion. 5. HTT significantly inhibited LD H activity in neuronal cells, suggesting a neuroprotection by HTT. 6. Serum interleukin $(IL)-1{\beta}$ and tumor necrosis factor $(TNF)-{\alpha}$ levels were significantly decreased in the femoral artery 1 hr after middle cerebral arterial occlusion in HTT-treated rats. IL-10 levels in the serum were significantly increased by HTT treatment whereas transforming growth factor $(TGF)-{\beta}$ levels were similar between HTT-treated and control groups. 7. Serum interleukin $(IL)-1{\beta}$ and tumor necrosis factor $(TNF)-{\alpha}$ levels were significantly decreased in the femoral artery 1 hr after reperfusion in HTT-treated rats. Serum IL-10 levels were significantly decreased in HTT-treated rats compared with the control group, and no significant changes in $(TGF)-{\beta}$ in the serum were observed by HTT treatment. Conclusions: The present data suggest that HTT may have an anti-ischemic effect via the improvement of cerebral hemodynamics and thus protect the brain from ischemic damage.

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Potent HAT Inhibitory Effect of Aqueous Extract from Bellflower (Platycodon grandiflorum) Roots on Androgen Receptor-mediated Transcriptional Regulation

  • Lee, Yoo-Hyun;Kim, Yong-Jun;Kim, Ha-Il;Cho, Hong-Yon;Yoon, Ho-Geun
    • Food Science and Biotechnology
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    • v.16 no.3
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    • pp.457-462
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    • 2007
  • Histone acetyltransferase (HAT) is a family of enzymes that regulate histone acetylation. Dysfunction of HAT plays a critical role in the development of cancer. Here we have screened the various plant extracts to find out the potent HAT inhibitors. The bellflower (Platycodon grandiflorum) root have exhibited approximately 30% of the inhibitory effects on HAT activity, especially p300 and CBP (CREB-binding protein) at the concentration of $100\;{\mu}g/mL$. The cell viability was decreased approximately 52% in LNCaP cell for 48 hr incubation. Furthermore, mRNA level of 3 androgen receptor target genes, PSA, NKX3.1, and TSC22 were decreased with bellflower root extract treatment ($100\;{\mu}g/mL$) in the presence of androgen. In ChIP assay, the acetylation of histone H3 and H4 in PSA promoter region was dramatically repressed by bellflower root treatment, but not TR target gene, Dl. Therefore, the potent HAT inhibitory effect of bellflower root led to the decreased transcription of AR target genes and prostate cancer cell growth with the repression of histone hyperacetylation.

Economic Dispatch Improvement Using The Massage Exchange (급전지시시스템(MX)을 활용한 경계급전 제고 방안)

  • Kim, Cheol-Sik;Sa, Gwan-Joo;An, Jae-Seung;Park, Bong-Yong
    • Proceedings of the KIEE Conference
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    • 2007.11b
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    • pp.9-11
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    • 2007
  • 우리나라는 정부의 전력시장 구조개편 계획에 의거 2001년부터 전력시장을 도입하도록 하는 결정에 따라 초기단계 전력시장을 운영하기 위한 "발전경쟁 (CBP Cost Based Pool)시스템"을 국내기술로 개발하여 현재까지 운영중에 있다. 또한 단계적인 시장도입 정책에 따라 2004년 4일부터 운영할 예정이었던 전력시장운영시스템(MOS : Market Operating System)은 정부의 도매전력시장 개설중단이란 정책변경으로 우수한 성능과 기능을 보유하고 있음에도 불구하고 활용이 불투명하기 도 하였으나, 2007년 세계최초로 발전경쟁시장시스템에 접목하여 MOS 상업운전을 개시하여 경제급전 효과를 극대화 할 수 있게 되었다. 경제급전 및 계통안정운영을 위한 MOS 시스템의 기능중 하나인 급전지시시스템(MX : Massage Exchange)은 미국 ABB사에서 개발하였던 초기의 기능은 매우 초보적인 단계로 단순히 5분 에너지 급전지시값만 전달하는 것으로서 국내 급전운영체계의 반영이 미흡하였다. 이에 따라, 2005년 국내기술로 초기 시스템을 개발한 이후 2007년에는 에너지 급전지시값 외에 보조서비스 데이터 제공 등의 성능 개선을 통해 중앙급전소 급전원, 현장 발전운전원 등에게 정확하고 신속한 계통운영관련 DATA를 실시간으로 제공하여, 계통운전에 활용함으로서 전력계통의 안정운영과 효율적인 시장운영에 많은 도움을 주고 있다. 본 논문에서는 이와 같이 완벽한 계통운전의 실현과 효율적 시장운영에 중요한 요소로서 역할을 수행하고 있는 급전지시 시스템의 개요와 주요 내용에 대해 살펴보도록 하겠다.

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Development of an Integrated Power Market Simulator for the Korean Electricity Market

  • Hur Jin;Kang Dong-Joo;Moon Young-Hwan
    • KIEE International Transactions on Power Engineering
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    • v.5A no.4
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    • pp.416-424
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    • 2005
  • At present, the Korean electricity industry is undergoing restructuring and the Cost Based-generation Pool (CBP) market is being operated in preparation of a Two Way Bidding Pool (TWBP) market. In deregulated electricity industries, an integrated power market simulator is one of the tools that can be used by market participants and market operators analyzing market behaviors and studying market structures and market codes. In this regard, it is very important to develop an electricity market simulator that reflects market code providing a market operation mechanism. This paper presents the development of an integrated market simulator, called the Power Exchange Simulator (PEXSIM), which is designed to imitate the Korean electricity market considering the various features of the market operating mechanism such as uniform price and constrained on/off payment. The PEXSIM is developed in VB.NET and composed of five modules whose titles are M-SIM, P-SIM, O-SIM, T-SIM and G-SIM interfacing the Access database program. To verify the features and the performance of the PEXSIM, a small Two Way bidding market with a 12-bus system and a One Way bidding market for generator competition will be presented for the electricity market simulations using PEXSIM.