• The Plant Pathology Journal
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• 제28권4호
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• pp.401-408
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• 2012

Initial subcellular responses in susceptible (PI 274420) and resistant (cv. Hartwig) soybeans infected with the soybean cyst nematode (SCN) were examined 2 and 4 days after inoculation (DAI). Subcellular features common to both soybeans at 2 DAI included hypertrophied initial syncytial cells (ISCs) and syncytium-component cells (SCs) with a dense cytoplasm containing proliferated rough and smooth endoplasmic reticulum (RER and SER), a hypertrophied nucleolus, and reduced vacuoles, suggesting that the nematode-infected cells were dedifferentiated. In the resistant soybean, a striking initial subcellular difference from the susceptible soybean was the dilation of the RER, indicating ER dysfunction and leading to cell death. This disturbed nematode feeding, as evidenced by disrupted feeding tubes. In PI 274420, the ISC cytoplasm was depleted, with the exception of ER membranes, at 4 DAI, while the SC cytoplasm was dense with proliferation of starch-containing plastids around multiple nuclei that might be derived from the congregation of nuclei in the neighboring SCs and in part by nuclear division without cytokinesis. In cv. Hartwig, syncytia were necrotized with secondary cell wall thickening outside the plasma membrane and an extremely dense cytoplasm containing a nucleus with an electron-lucent nucleolus, accompanied by the proliferation of closely stacked parallel RER and ribosomes. These results suggest that syncytia develop continuously in PI 274420 to produce and store nutritional substances in SCs, providing for the nematode through ISC until maturation, but in cv. Hartwig, syncytia degenerate early due to excessive metabolism, blocking nematode feeding and cytoplasmic connections with adjacent intact cells.

• 생명과학회지
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• 제32권6호
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• pp.421-429
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• 2022

본 연구에서는 죽상경화 플락에서 발견되는 펩티도글리칸이 혈관염증에서 어떠한 역할을 하는지 알아보기 위하여 염증성 사이토카인의 한 종류인 인터루킨-1 알파의 발현에 대한 영향을 조사하였다. 실험방법으로는 혈관염증을 주도하는 단핵구/대식세포인 THP-1 세포주에 펩티도글리칸을 처리하고 인터루킨-1 알파의 발현을 RT-PCR, real-time PCR, ELISA 방법으로 분석하였다. 펩티도글리칸의 처리 시간과 농도에 비례하여 단핵구/대식세포에서 인터루킨-1 알파의 전사체와 단백질 분비가 증가함을 관찰하였다. 또한 펩티도글리칸의 작용기전을 규명하기 위하여 신호전달을 차단하는 억제제를 세포에 처리하고 인터루킨-1 알파의 발현을 조사하였다. TLR2/4의 억제제인 OxPAPC 그리고 세포 kinase의 작용을 억제하는 LY294002(PI3 kinase 억제), Akti IV (Akt 억제), rapamycin (mTOR 억제), U0126 (MEK 억제), SB202190 (p38 MAPK 억제), SP6001250 (JNK 억제), DPI (NOX 억제)를 처리하는 경우 인터루킨-1 알파 전사체의 발현 그리고 단백질의 분비가 감소되었다. 반면에 LPS의 작용을 억제하는 polymyxin B는 인터루킨-1 알파의 발현에 영향을 주지 않았다. 이상의 결과는, 펩티도글리칸이 TLR2, PI3K, Akt, mTOR, MAPKs를 통하여 단핵구/대식세포의 인터루킨-1 알파 발현을 증가시키고 혈관염증에 기여한다는 것을 나타낸다.

• 사상체질의학회지
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• 제33권3호
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• pp.127-137
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• 2021

Objectives The purpose of this study was to identify the effects of personality by each Sasang constitution on sleep using Positive Affect and Negative Affect Schedule(PANAS) and NEO-personality inventory(NEO-PI). Methods The subjects of this study were 2,078 people who had information on Sasang constitutional type and personality(PANAS, NEO-PI) among the data established at the Korean Medicine Data Center. Sleep time and sleep quality were investigated through self-written questionnaires. Sleep time was collected by hand-writing, and sleep quality was checked on a 4 point Likert scale. Pearson correlation analysis was conducted to see the correlation between personality and sleep in each constitutional type. Logistic regression was performed using personality as independent variables to find out how much personality affects sleep time. In order to find out how much personality affects sleep quality, regression analysis was performed using personality as independent variables. Results & Conclusions Sleep time was hardly affected by personality. As a result of Pearson correlation analysis, sleep time in all subjects did not show a significant correlation with personality. In logistic regression on sleep time as the dependent variable, no statistically significant results were obtained except for the Negative Affect(NA) in Taeeumin. Sleep quality showed a statistically significant correlation with the negative affect(NA), neuroticism(N), extraversion(E), Physical Component Summary(PCS) and Mental Component Summary(MCS). As a result of regression analysis on sleep quality as the dependent variable, neuroticism(N), negative affect(NA), positive affect(PA), and extraversion(E) were found in the factors affecting sleep quality. Besides, how much personality affected sleep quality might differ in each constitution. In all constitutions, sleep quality was affected by N, but the rank of N was different in each constitution. The sleep quality of Soyangin was not affected by E, and the sleep quality of Taeeumin was specifically affected by O.

• 한국식품과학회지
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• 제11권2호
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• pp.118-121
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• 1979

Under the experimental conditions of varying the thickness(2L cm) of ginseng disc and the bulk, concentration $(C_b.{\;}%)$, the experimental results were well coincided with those of the model which was set up by using the inter disc diffusion with constant diffusivity. It was therefore convinced that the extraction rate is controlled by the diffusion rate of ginseng components through the disc. The relationship between the diffusivity$(D{\;}cm^2/min)$ and the mean concentration (C %) of soluble ginseng components in the disc is as follows, $ln{\frac{C-C_{b0}}{C_i-C_{b0}}}=ln{\frac{8}{{\pi}^2}-{\frac{{\pi}^2D}{4L^2}{\theta}$ where $C_i(%)$ is the initial concentration of soluble ginseng components and $\theta$ (min.) is the extraction time.

• 대한방사선기술학회지:방사선기술과학
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• 제41권1호
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• pp.13-24
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• 2018

Liver size is an important component in the diagnosis and follow-up of diffuse liver disease when testing for liver disease using ultrasonography. However, difficulties lies in determining the presence of hepatomegaly and liver atrophy because the method used for measuring liver size differs from one examiner to another and there is no relevant standard based on body build. The present study aims to propose a more objective method for liver size measurement and a reference range based on body build. A total of 260 normal adults (130 males, 130 females) participated in the study. Ultrasonography was performed in all participants to measure the size of the right lobe, left lobe, quadrate lobe, and caudate lobe of liver. Based on Physique Index(PI), a value derived from multiplying weight(kg) by height($m^2$), size of physique was divided into three groups including Group I with PI<150, Group II with $150{\leq}PI{\leq}250$, and Group III with PI>250. Thus, mean liver size by PI and a reference range with 95% reliability were suggested. The superoinferior diameter of right lobe was $12.34{\pm}1.18cm$ in males and $11.07{\pm}0.93cm$ in females, and its reference range was 10.64~11.0cm for Group I, 11.78~12.12cm for Group II, and 13.02~13.84cm for Group III. The anteroposterior diameter(T) of left lobe was $5.93{\pm}1.09cm$ in males and $5.18{\pm}0.99cm$ in females, and its reference range was 4.77~5.17cm for Group I, 5.49~5.79cm for Group II, and 6.68~7.44cm for Group III. The transverse diameter was $3.51{\pm}0.60cm$ in male participants and $3.42{\pm}0.49cm$ in female participants and its reference range was 3.29~3.51cm for Group I, 3.36~3.55cm for Group II, and 3.52~4.0cm for Group III. The caudate lobe index was $11.65{\pm}2.88cm^2$ in males and $9.62{\pm}2.18cm^2$ in females and its reference range was $8.83{\sim}9.75cm^2$ for Group I, $10.62{\sim}11.47cm^2$ for Group II, and $11.89{\sim}14.26cm^2$ for Group III. As a basic measurement method of liver size, the present study suggests measuring the superoinferior diameter for right liver lobe, the anteroposterior diameter for left liver lobe, the transverse diameter for quadrate lobe, and the caudate lobe index for caudate lobe. It is expected that this method along with its relevant reference range can be used as useful indicators in determining hepatomegaly and liver atrophy upon the diagnosis and follow-up testing of diffuse liver disease.

• Journal of Ginseng Research
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• 제47권3호
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• pp.420-428
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• 2023

Background: Ginsenoside F2 (GF2), a minor component of Panax ginseng, has been reported to possess a wide variety of pharmacological activities. However, its effects on glucose metabolism have not yet been reported. Here, we investigated the underlying signaling pathways involved in its effects on hepatic glucose. Methods: HepG2 cells were used to establish insulin-resistant (IR) model and treated with GF2. Cell viability and glucose uptake-related genes were also examined by real-time PCR and immunoblots. Results: Cell viability assays showed that GF2 up to 50 μM did not affect normal and IR-HepG2 cell viability. GF2 reduced oxidative stress by inhibiting phosphorylation of the mitogen-activated protein kinases (MAPK) signaling components such as c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase 1/2 (ERK1/2), and p38 MAPK, and reducing the nuclear translocation of NF-κB. Furthermore, GF2 activated PI3K/AKT signaling, upregulated the levels of glucose transporter 2 (GLUT-2) and GLUT-4 in IR-HepG2 cells, and promoted glucose absorption. At the same time, GF2 reduced phosphoenolpyruvate carboxykinase and glucose-6-phosphatase expression as well as inhibiting gluconeogenesis. Conclusion: Overall, GF2 improved glucose metabolism disorders by reducing cellular oxidative stress in IR-HepG2 cells via MAPK signaling, participating in the PI3K/AKT/GSK-3β signaling pathway, promoting glycogen synthesis, and inhibiting gluconeogenesis.

• 반도체디스플레이기술학회지
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• 제19권4호
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• pp.130-134
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• 2020

This paper proposes a new method for the digit recognition on a 7-segment display. The proposed method uses morphological processing that dilates segments of digits and connects them into strokes. The digits are extracted by connected component analysis and finally, template matching method recognizes the extracted digits. The proposed method is implemented using C language in Raspberry Pi 4 system with a camera module for a real-time image processing. Experiments were conducted by using various 7-segment LED displays and 7-segment mono LCD displays. The results show that the proposed method is successful for the digit recognition on the 7-segment displays.

• 천문학회지
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• 제38권2호
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• pp.219-222
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• 2005

We present the high-resolution (2"-4") images of the molecular envelopes surrounding the evolved stars, V Hya, VY CMa, and ${\pi}^1$ Gru observed with the Submillimeter Array. The CO J=2-1 and 3-2 images of the carbon star V Hya show that the circumstellar structure of this star consists of three kinematic components; there is a flattened disk-like envelope that is expanding with a velocity of ${\~}16 km\;s^{-1}$, the second component is the medium-velocity wind having a deprojected velocity of 40-120 km $s^{-l}$ moving along the disk plane, and the third one is the bipolar molecular jet having an extreme velocity of 70-185 km $s^{-l}$. The axis of this high velocity jet is perpendicular to the plane of the disk-like envelope. We found that the circumstellar structure of the S-star ${\pi}^1$ Gru traced by the CO J =2-1 resembles that of V Hya quite closely; the star is surrounded by the expanding disk-like envelope and is driving the medium-velocity wind along the disk plane. We also obtained the excellent images of VY CMa with the CO and $^{13}CO$ J=2-1 and $SO\;6_5-5_4$ lines. The maps of three molecular lines show that the envelope has a significant velocity gradient in the east-west direction, suggesting that the envelope surrounding VY CMa is also flattened and expanding along its radial direction. The high-resolution images obtained with the SMA show that some AGB stars are associated with the asymmetric mass loss including the equatorial wind and bipolar jet.

• 전기학회논문지P
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• 제63권4호
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• pp.219-225
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• 2014

The purpose of this paper is to control of the low-speed, high-precision PMSM 2-axes pitch/turning. In this paper, apply the PAM-PWM inverter for it. However, The PAM-PWM inverter, control algorithms and hardware is complex. But it is possible to improve the performance in the low-speed operation can reduce the effect of the PWM ripple and Dead Time of inverter by applying suitable DC-bus voltage control. The direct driver PMSM(Permanent Magnet Synchronous Motor) configured to vector control part, PAM control part and the other controller. The vector control part includes PI current, speed control, additional space vector modulation. PAM control part has to have PI voltage controller and P current controller for DC-bus voltage control. Besides, the motor position estimator, the speed estimator and the counter electromotive force and Dead Time Compensation are added. With this arrangement, PMSM was driven with a low pole pitch/turning by performing the current control to the current command or torque command is the paper. As a result, it was possible to minimize the disturbance component that appears in the drive in proportion to the DC voltage magnitude. The use of a hydraulic drive method for a two-axis bubble column is a typical tank. When using the PWM PAM inverter driver is in the turret can be driven by high-precision, low vibration, low noise compared to the hydraulic drive may contribute to the computerization of the turret.

• Bulletin of the Korean Chemical Society
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• 제25권5호
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• pp.704-710
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• 2004

A poly(1-hexyl-3,4-dimethyl-2,5-pyrrolylene) (PHDP) was prepared and its luminescence in tetrahydrofuran (THF) was studied. When PHDP is excited by UV light, it produces very strong blue luminescence. The quantum yield of PHDP (Q = 36.9%) is much greater than that of the monomer, 1-hexyl-3,4-dimethylpyrrole (HDP) with Q = 0.61%. The principal luminescence of PHDP has a single decay component with ca. 1 ns, whereas the decay of HDP is complicated. The molecular structure and conformational behavior of HDP and the oligomers up to trimer have been also determined by ab initio Hartree-Fock (HF/6-31$G^{**}$), density functional theory (DFT-B3LYP/6-31$G^{**}$), and semiempirical (ZINDO) methods. According to the results of calculations, it is proposed that the enhanced quantum yield of the polymer PHDP results mostly from the ${\pi}$-conjugation between neighboring pyrrole rings.