• Title/Summary/Keyword: 4-chlorophenoxyacetic acid

Search Result 8, Processing Time 0.023 seconds

Characteristics of Pseudomonas sp. degrading 2-methyl-4-chlorophenoxyacetic acid (2-메틸-4-클로로페녹시 아세트산을 분해하는 Pseudomonas 균주의 특성)

  • 은성호;박영두;이영록
    • Korean Journal of Microbiology
    • /
    • v.24 no.4
    • /
    • pp.389-393
    • /
    • 1986
  • From the soil and river samples, some bacterial strains degrading chlorinated aromatic hydrocarbons were isolated and identified. Of the isolates, seven strains of Pseudomonas sp. harbouring plasmids were selected for their prominent degradative ability to 2-methyl-4-chlorophenoxyacetic acid. By agarose gel electrophoresis and curing experiment it was found that the genes for 2-methyl-4-chlorophenoxyacetic acid degradaiton were encoded on the plasmids in these selected strains. Antobiotic resistance and degradative ability for other herbicides of the strains were tested.

  • PDF

Isolation and Characterization of 2-Methyl-4-Chlorophenoxyacetic Acid-Degrading Bacteria from Agricultural Soils

  • Cho, Seung-Hee;Ka, Jong-Ok
    • Journal of Applied Biological Chemistry
    • /
    • v.42 no.2
    • /
    • pp.57-61
    • /
    • 1999
  • Seven numerically dominant 2-methyl-4-chlorophenoxyacetic acid (MCPA)-degrading bacteria were isolated from agricultural soils. The isolates utilized the herbicide MCPA as a sole carbon source, producing significant biomass in MCPA mineral medium. They exhibited diverse herbicide degradation capabilities, but most of them grew very slowly in mineral medium containing herbicide. The chromosomal DNA patterns of the isolates obtained by polymerase chain reaction amplification of repetitive extragenic palindromic sequences were distinct from each other. One isolate, SH3, which was identified as Sphingomonas species by fatty acid methyl ester analysis, was able to degrade 5 different phenoxyacetic acid herbicides within 4 days. This strain contains two plasmids, and the smaller one has a crucial role in herbicide degradation. MCPA treated into agricultural soils without indigenous MCPA-degraders persisted for a long time, but the application of the isolate SH3 resulted in rapid decline of MCPA concentration in the soil.

  • PDF

Isolation and characterization of 4-chlorophenoxyacetic acid-degrading bacteria from agricultural soils

  • Chung, Min-Jae;Shin, Se-Young;Park, Yong-Keun;Min, Kyung-Hee;Ka, Jong-Ok
    • Journal of Microbiology
    • /
    • v.35 no.2
    • /
    • pp.117-122
    • /
    • 1997
  • Several dominant 4-CPA-degrading bacteria were isoalted from agricultural soils. Most of the isolates were identified as Burkholderia species by fatty acid methyl ester (FAME) analysis, but they were idstinct in chromosomal patterns obtained by PCR amplification of repetitive extragenic palindromic (REP) sequences. These strains were generally restricted in their substrate utilization capabilities. The 4-CPA degradative enzymes were idnducible by 4-CPA and some isolates appeared to mineralize 4-CPA via formation of 4-chlorophenol and 4-chlorocatechol as intermediates during its biodegradation pathway. Plasmid DNAs were not detected from most of the isoaltes and their 4-CPA genes wer on the chromosomal DAN. The 4-CPA degradation patterns in axenic cultures and natural soils varied depending on the strains and soils. The inoculation of 4-CPA degraders much improved the removal of 4-CPA from the 4-CPA treated soils.

  • PDF

Enhanced Production of Shikonin by Using Polyurethane-entrapped Lithospermum erythrorhizon Cells (Polyurethane Foam 에 포괄시킨 Lithospermum erythrorhizon 세포에 의한 Shikonin 생산)

  • Taek, Seo-Weon;Liu, Jang-Ryol;Park, Young-Hoon
    • Microbiology and Biotechnology Letters
    • /
    • v.17 no.4
    • /
    • pp.343-348
    • /
    • 1989
  • Production of shikonin derivatives by Lithospermum erythrorhizon cells by using polyurethane foam was invesliigated. Shikonin derivatives were effectively adsorbed mostly by phase distribution to polyurethane matrices and their production increased significantly compared to the suspension culture. The enhanced production of shikonin was probably due to more facilitated cell to cell con-tact and lowered intracellular shikonin concentration, both of which are known to be favorable for plant secondary metabolite production. In order to improve the process productivity, tell culture was conducted under various culture conditions: Of them, Schenk and Hildebrandt medium containing indole-3-acetic acid (1.75mg/ι) and kinetin (0.1mg/ι) was considered most appropriate for shikonin production. Production of shikonin increased about 4.5 times in the Schenk and Hildebrandt medium containing indole-3-acetic acid (1.15mg/ι) and kinetin (0.1mg/ι) when compared to the same medium containing p-chlorophenoxyacetic acid (2.0mg/ι) and kinetin (0.1mg/ι). When poly-urethane was used as the support material, a single-stage system was more preferred to the conventional two-stage culture system in terms of shikonin productivity.

  • PDF

Genetic and Phenotypic Diversity of Dichlorprop-Degrading Bacteria Isolated from Soils

  • Park, Hae-Dong;Ka, Jong-Ok
    • Journal of Microbiology
    • /
    • v.41 no.1
    • /
    • pp.7-15
    • /
    • 2003
  • Nine dichlorprop-degrading bacteria and three pairs of bacteria showing syntrophic metabolism of the herbicide were isolated from soils, and their genetic and phenotypic characteristics were investigated. Analysis of 16S rDNA sequences indicated that the isolates were related to members of the genera, Sphingomonas, Herbaspirillum, and Bradyrhizobium. Twelve different chromosomal DNA patterns were obtained by polymerase-chain-reaction (PCR) amplification of repetitive extragenic palindromic (REP) sequences from the 15 isolates. The isolates were able to utilize the herbicide dichlorprop as a sole source of carbon and energy and their dichlorprop derogative pathways were induced by the presence of dichlorprop. Most of the isolates and syntrophic pairs were able to degrade both (R)- and (S)-dichlorprop, but strain DP522 exhibited enantioselective degradation of (S)-dichlorprop. The isolates degraded 2,4-dichlorophenoxyacetic acid, 2-methyl-4-chlorophenoxyacetic acid , and mecoprop, in addition to dichlorprop. Oxygen uptake experiments indicated that most of the isolates degraded dichlorprop through 2,4-dichlorophenol.

Initiation of embryogenic callus from mature zygotic embryos in Japanese larch (Larix kaempferi)

  • Kim, Yong-Wook
    • Journal of Plant Biotechnology
    • /
    • v.42 no.3
    • /
    • pp.223-227
    • /
    • 2015
  • Embryogenic callus (EC) was created from mature embryos of Larix kaempferi. With the mature embryos, keeping the culture in dark conditions throughout the experiment (38.2%) seemed to give better results than exposing them to 16 h light ($25{\mu}Em^{-2}s^{-1}$) for the first week (21.9%). EC was obtained most frequently from Quoirin and Lepoivre (LP) mediums with 1.0 mg/L 4-amino-3,5,6-trichloropicolinic acid (Picloram), plus 1.0 mg/L benzyladenine (BA) (62.8%) or Litvay's medium (LM) containing 1.0 mg/L p-chlorophenoxyacetic acid (pCPA) plus 1.0 mg/L BA (62.8%) treatment. In both cases, best results were obtained when zygotic embryos were cultured in darkness. As for the effective sucrose concentration on initiation of EC, 29.2 mM sucrose (38.6%) gave the best results.

A Case of Bentazone Poisoning Mimicking Organophosphate Intoxication (유기인산 화합물 중독으로 의심했던 벤타존 중독 1례)

  • Jung, Hyun-Min;Kim, Ji-Hye;Han, Seung-Baik;Paik, Jin-Hui;Kim, Ji-Yoon;Kim, Jun-Sig
    • Journal of The Korean Society of Clinical Toxicology
    • /
    • v.8 no.2
    • /
    • pp.122-124
    • /
    • 2010
  • $Basagran^{(R)}$ is a herbicide that is widely used in the field and it acts by interfering with photosynthesis in plants. It consists of bentazone, 2-methyl-4-chlorophenoxyacetic acid (MCPA) and surfactants. Bentazone is commonly used, but poisoning due to Bentazone has not been previously reported in Korea. The patients with toxic effects of bentazone show mild to severe symptoms and various complications. We report here on a case of a woman who intentionally ingested 500 ml of $Basagran^{(R)}$ and she was discharged without complication. As soon as the patient visited the emergency department, we started to treat her as if she had organophosphate intoxication because of the cholinergic symptoms. We could not detect the bentazone in her serum and urine, and we could confirm $Basagran^{(R)}$ ingestion only after getting information from her husband. Bentazone poisoning may induce harmful complications like muscle rigidity, rhabdomyolysis, respiratory failure and cardiac arrest. A detailed history taking, an accurate analysis method and early conservative management will be helpful for patients with acute bentazone poisoning.

  • PDF